##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265914 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477699117001627 31.0 31.0 34.0 30.0 34.0 2 31.5490617846078 33.0 31.0 34.0 30.0 34.0 3 31.502454353139314 33.0 31.0 34.0 28.0 34.0 4 35.1564079392439 37.0 35.0 37.0 33.0 37.0 5 35.205939739982334 37.0 35.0 37.0 33.0 37.0 6 35.26792341343883 37.0 35.0 37.0 33.0 37.0 7 35.14871942327836 37.0 35.0 37.0 32.0 37.0 8 35.1385157285566 37.0 35.0 37.0 32.0 37.0 9 36.64802901302932 39.0 37.0 39.0 32.0 39.0 10-11 36.53076828283754 39.0 37.0 39.0 32.0 39.0 12-13 36.448219626293735 39.0 37.0 39.0 32.0 39.0 14-15 37.431318004224615 40.0 37.0 41.0 32.0 41.0 16-17 37.362689724578445 40.0 37.0 41.0 32.0 41.0 18-19 37.27556690264899 40.0 37.0 41.0 32.0 41.0 20-21 37.2653679475857 39.5 37.0 41.0 31.5 41.0 22-23 37.255361343661576 39.0 36.5 41.0 31.5 41.0 24-25 37.370488437603186 40.0 37.0 41.0 32.0 41.0 26-27 37.28543131681931 39.5 37.0 41.0 31.5 41.0 28-29 37.17572007261157 39.0 36.5 41.0 31.0 41.0 30-31 37.00862973314143 39.0 36.0 41.0 31.0 41.0 32-33 36.846734454315225 39.0 36.0 41.0 30.0 41.0 34-35 36.65466966950362 39.0 35.5 41.0 30.0 41.0 36-37 36.435195044845074 39.0 35.0 40.0 30.0 41.0 38-39 36.239562877099075 39.0 35.0 40.0 30.0 41.0 40-41 36.035953074221474 38.5 35.0 40.0 29.5 41.0 42-43 35.78569594775 38.0 35.0 40.0 28.5 41.0 44-45 35.628110993321826 38.0 35.0 40.0 28.0 41.0 46-47 35.468429134996526 38.5 35.0 40.0 27.0 41.0 48-49 35.235464257445614 38.5 35.0 40.0 26.0 41.0 50-51 34.27258012787598 37.5 33.5 39.5 25.0 40.5 52-53 34.384871721143774 38.0 33.5 39.5 24.5 40.5 54-55 34.84559693628477 38.0 34.0 40.0 26.0 41.0 56-57 34.702994042249315 38.0 34.0 40.0 24.5 41.0 58-59 34.504749137777125 38.0 34.0 40.0 24.0 41.0 60-61 34.20041724793312 38.0 34.0 40.0 23.0 41.0 62-63 33.87568468316173 37.5 33.0 40.0 22.5 41.0 64-65 33.51964548934603 37.0 33.0 40.0 22.0 41.0 66-67 33.152291940842744 36.0 32.0 39.0 21.5 41.0 68-69 32.73010923332865 36.0 32.0 39.0 20.0 40.5 70-71 32.311745505618866 35.0 31.5 39.0 18.5 40.0 72-73 31.878692391426274 35.0 31.0 38.0 18.0 40.0 74-75 31.45267727507556 35.0 31.0 37.0 14.5 39.5 76-77 30.97369015588737 35.0 30.5 37.0 11.0 39.0 78-79 30.493900849504783 34.5 30.0 36.0 7.5 39.0 80-81 30.022652012695964 34.0 29.5 36.0 7.0 37.5 82-83 29.596236000233823 34.0 29.0 35.0 2.0 37.0 84-85 29.210419112198775 34.0 29.0 35.0 2.0 37.0 86-87 28.855976393341095 34.0 29.0 35.0 2.0 36.0 88-89 28.475480166899175 34.0 29.0 35.0 2.0 36.0 90-91 28.083968579224184 34.0 27.5 35.0 2.0 35.5 92-93 27.658140284411104 34.0 27.0 35.0 2.0 35.0 94-95 27.193910881781857 33.0 26.0 35.0 2.0 35.0 96-97 26.611888722298673 33.0 25.0 35.0 2.0 35.0 98-99 25.88127945500247 33.0 24.0 35.0 2.0 35.0 100-101 23.350938926340966 29.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4226717766927166 1101 2 0.3314109244793073 1101 3 0.32003814162287014 1101 4 0.31303501227855435 1101 5 0.3098608023883287 1101 6 0.2627655002006293 1101 7 0.1439744836022001 1101 8 0.31044402281926153 1101 9 0.5460922402053257 1101 10-11 0.46521484137880265 1101 12-13 0.3691341603712246 1101 14-15 0.5393875498615017 1101 16-17 0.5298694209338493 1101 18-19 0.7426129358696301 1101 20-21 0.5572358402842923 1101 22-23 0.5782440942001799 1101 24-25 0.45871211709853554 1101 26-27 0.43722115332530365 1101 28-29 0.34082076736932265 1101 30-31 0.20823415119985356 1101 32-33 0.2696976400950106 1101 34-35 0.4704805007896695 1101 36-37 0.4593301646796064 1101 38-39 0.583971719344099 1101 40-41 0.4514918390463194 1101 42-43 0.5623893243469666 1101 44-45 0.5467825831049282 1101 46-47 0.5724153694410035 1101 48-49 0.6271436982528016 1101 50-51 0.5437705572473206 1101 52-53 0.6311780401099085 1101 54-55 0.7080454190216869 1101 56-57 0.7228594324437196 1101 58-59 0.7494132751913938 1101 60-61 0.6037546920002299 1101 62-63 0.6797425682676135 1101 64-65 0.4782434723945954 1101 66-67 0.6324374086248383 1101 68-69 0.7001073975346017 1101 70-71 0.6184964003860358 1101 72-73 0.7791592915026762 1101 74-75 0.43777862741157847 1101 76-77 0.550794341203332 1101 78-79 0.5994292818035909 1101 80-81 0.6760087086689488 1101 82-83 0.7398313809558807 1101 84-85 0.6498962769664516 1101 86-87 0.5803619712758561 1101 88-89 0.5211795795701093 1101 90-91 0.3736183614264412 1101 92-93 0.36274189611919994 1101 94-95 0.3803146451551278 1101 96-97 0.4584145965893569 1101 98-99 0.4649137850465159 1101 100-101 0.9799416942750234 1102 1 0.13265749147460326 1102 2 0.3585776474538811 1102 3 0.5393092481792294 1102 4 0.4091104338999756 1102 5 0.4118731372028108 1102 6 0.3263484782829522 1102 7 0.5312847714400988 1102 8 0.27331824298482843 1102 9 0.39552685319409164 1102 10-11 0.38502862384023473 1102 12-13 0.4427648295211455 1102 14-15 0.6355682950834876 1102 16-17 0.5245423471753128 1102 18-19 0.5991921877067057 1102 20-21 0.6827369167501303 1102 22-23 0.41350828503301074 1102 24-25 0.5516066306636347 1102 26-27 0.4461274252442351 1102 28-29 0.2677444566994325 1102 30-31 0.4909632909248032 1102 32-33 0.5041867665045316 1102 34-35 0.25381542036709703 1102 36-37 0.29110717454484103 1102 38-39 0.27162973293648207 1102 40-41 0.4784754297646714 1102 42-43 0.26671959029018666 1102 44-45 0.5438017222299649 1102 46-47 0.5529886906797898 1102 48-49 0.6789302075399988 1102 50-51 0.5425594486070437 1102 52-53 0.23166306995008767 1102 54-55 0.4538131919845938 1102 56-57 0.39966640879034543 1102 58-59 0.29017373414068004 1102 60-61 0.3496171885439452 1102 62-63 0.2786569909251284 1102 64-65 0.4024093523292933 1102 66-67 0.1784956221290841 1102 68-69 0.19982119700117806 1102 70-71 0.0021250983833596138 1102 72-73 0.3107108141391315 1102 74-75 0.304033441963103 1102 76-77 0.520848834326177 1102 78-79 0.19225365481242562 1102 80-81 0.4304175275245363 1102 82-83 0.3458879616060173 1102 84-85 0.3981593396865435 1102 86-87 0.3053160416157823 1102 88-89 0.46318190977019924 1102 90-91 0.5535320456910959 1102 92-93 0.47631569503547055 1102 94-95 0.6713372854441353 1102 96-97 0.5286573189991977 1102 98-99 0.5853399329046134 1102 100-101 1.0210436091798663 1103 1 0.30548726395588943 1103 2 0.3595228862405584 1103 3 0.23932208205154382 1103 4 0.34780394569860107 1103 5 0.46166067748386297 1103 6 0.4144002307635404 1103 7 0.45724208852127646 1103 8 0.2203734825117536 1103 9 0.2642617443755597 1103 10-11 0.3431509976598406 1103 12-13 0.24380170128010548 1103 14-15 0.4700844909485298 1103 16-17 0.689308899913982 1103 18-19 0.785948463711172 1103 20-21 0.6124789121150442 1103 22-23 0.36945021460790173 1103 24-25 0.3839566178078613 1103 26-27 0.40851760912777735 1103 28-29 0.3782892129675588 1103 30-31 0.46356406857862 1103 32-33 0.5281515044548186 1103 34-35 0.4322537702552509 1103 36-37 0.30339878290060796 1103 38-39 0.1170553772910452 1103 40-41 0.40585586121930817 1103 42-43 0.35892636368385666 1103 44-45 0.5342122353176677 1103 46-47 0.6677195670054985 1103 48-49 0.5915911072864262 1103 50-51 0.5507838582817683 1103 52-53 0.6051105765652309 1103 54-55 0.678171618823427 1103 56-57 0.851421780291524 1103 58-59 0.6350144079671551 1103 60-61 0.6174447731347144 1103 62-63 0.7104434265295367 1103 64-65 0.6316954605166458 1103 66-67 0.7324228527590222 1103 68-69 0.8654344348995622 1103 70-71 0.7138386568657396 1103 72-73 0.615445417982027 1103 74-75 0.5101798155619441 1103 76-77 0.4566753116121909 1103 78-79 0.5752262599702398 1103 80-81 0.6314156786584952 1103 82-83 0.42678077862346697 1103 84-85 0.5535311036782034 1103 86-87 0.5298706996763087 1103 88-89 0.3028912247524076 1103 90-91 0.4173456067577739 1103 92-93 0.5916173063653716 1103 94-95 0.6295558038020452 1103 96-97 0.6682850315782751 1103 98-99 0.7301292274402336 1103 100-101 1.1144621541691642 1104 1 0.18598735712566494 1104 2 0.16816359194008612 1104 3 0.47769168128087003 1104 4 0.4988910436182792 1104 5 0.3333760033847142 1104 6 0.2752243613840548 1104 7 0.18496440391327695 1104 8 0.34459648173475443 1104 9 0.44744099599372333 1104 10-11 0.5197538693984498 1104 12-13 0.5419665203725188 1104 14-15 0.4836132259139134 1104 16-17 0.3549049931118873 1104 18-19 0.40575029335463597 1104 20-21 0.5330648621127594 1104 22-23 0.3078118152998215 1104 24-25 0.4220103187574864 1104 26-27 0.04225117696767455 1104 28-29 0.1427212992322211 1104 30-31 0.046223019733041326 1104 32-33 0.11173182559169703 1104 34-35 0.2760572769546812 1104 36-37 0.20877169053596134 1104 38-39 0.202881002143819 1104 40-41 0.38797649322681593 1104 42-43 0.5073201079486438 1104 44-45 0.5808314029775588 1104 46-47 0.6860926908650526 1104 48-49 0.5943451091920124 1104 50-51 0.5094696546372504 1104 52-53 0.4306079801169247 1104 54-55 0.6058437455905761 1104 56-57 0.6991116093161978 1104 58-59 0.6177133957566454 1104 60-61 0.5325440341050083 1104 62-63 0.4940234400303609 1104 64-65 0.6537890029721822 1104 66-67 0.5085385168837391 1104 68-69 0.4201758536115605 1104 70-71 0.45207223701108035 1104 72-73 0.6288924096530017 1104 74-75 0.5996469723367923 1104 76-77 0.6812946779170801 1104 78-79 0.5451946086150592 1104 80-81 0.6208055308877114 1104 82-83 0.5007947171736049 1104 84-85 0.41698947788318463 1104 86-87 0.360037359311292 1104 88-89 0.3292918917073706 1104 90-91 0.30831807069403894 1104 92-93 0.2501502954979067 1104 94-95 0.47674330709814683 1104 96-97 0.29413630448837225 1104 98-99 0.15966738661640179 1104 100-101 0.8252565759364323 1105 1 0.24725621471117876 1105 2 0.19534957253453555 1105 3 0.3650048903367953 1105 4 0.38533678589139697 1105 5 0.4629685795825651 1105 6 0.2652743745923942 1105 7 0.36021857525046386 1105 8 0.4208344960568837 1105 9 0.24919717000113195 1105 10-11 0.33524850395576067 1105 12-13 0.29513027525425883 1105 14-15 0.5980550306732013 1105 16-17 0.5814405521103367 1105 18-19 0.37233408065035434 1105 20-21 0.355603648230435 1105 22-23 0.25745144175363066 1105 24-25 0.44144264563896485 1105 26-27 0.2975831525786532 1105 28-29 0.18375194258557315 1105 30-31 0.054582204528465184 1105 32-33 0.1562778513087011 1105 34-35 0.015206802525320029 1105 36-37 0.15325381643645386 1105 38-39 -0.0362533516826673 1105 40-41 0.35549021919299406 1105 42-43 0.10080705667885326 1105 44-45 0.25524220383145746 1105 46-47 0.6896870555437999 1105 48-49 0.8247088562201341 1105 50-51 0.7822781408184696 1105 52-53 0.9473325968965227 1105 54-55 0.6998496572160562 1105 56-57 0.7464501284111762 1105 58-59 0.7288250026763805 1105 60-61 0.5894670794488945 1105 62-63 0.8588391836600167 1105 64-65 0.8893508572806041 1105 66-67 0.7591007638725955 1105 68-69 0.7615637469167211 1105 70-71 0.7719332102873935 1105 72-73 0.6894156206366269 1105 74-75 0.5583343130334804 1105 76-77 0.7629824719811786 1105 78-79 0.5783015854754687 1105 80-81 0.8987701789389426 1105 82-83 1.0338347598686823 1105 84-85 0.7366786534684557 1105 86-87 0.8081316990012937 1105 88-89 0.7212061552887619 1105 90-91 0.720485274023968 1105 92-93 0.6412649189173685 1105 94-95 0.6758983301948973 1105 96-97 0.9489469968196822 1105 98-99 1.1834816701887938 1105 100-101 1.5632075337197158 1106 1 0.09374410176440051 1106 2 0.05670709262721729 1106 3 0.16219732960824373 1106 4 0.3482516653534944 1106 5 0.30857685710414984 1106 6 0.18737409764963076 1106 7 0.22164071228753102 1106 8 0.45130002018675697 1106 9 0.33193598194125684 1106 10-11 0.5312678726573168 1106 12-13 0.46359575023885213 1106 14-15 0.35526255026533704 1106 16-17 0.11439451117232124 1106 18-19 0.32315558043693215 1106 20-21 0.13523597781689745 1106 22-23 -0.045884385247696 1106 24-25 -0.23602619943782344 1106 26-27 -0.18955921311138724 1106 28-29 0.058722250644578367 1106 30-31 0.054525778464345365 1106 32-33 0.19909772566059303 1106 34-35 0.30498239970874863 1106 36-37 -0.012152819814744475 1106 38-39 -0.07592792800938497 1106 40-41 0.07143549683198103 1106 42-43 0.024443479885256636 1106 44-45 -0.02900295649592266 1106 46-47 0.02738567301422279 1106 48-49 0.0807248302568695 1106 50-51 -0.14538592023817642 1106 52-53 -0.033962845424667876 1106 54-55 0.12039561579022262 1106 56-57 0.21172921445455017 1106 58-59 0.09371529493609643 1106 60-61 0.2478300137134397 1106 62-63 0.15717083786289265 1106 64-65 -0.07977543972253898 1106 66-67 -0.1889478954168169 1106 68-69 -0.11120548959312515 1106 70-71 -0.2091071771230446 1106 72-73 -0.02916803054836592 1106 74-75 0.2147473627439318 1106 76-77 0.1239732226571526 1106 78-79 0.3019317290069985 1106 80-81 0.2830958374174628 1106 82-83 0.5069891497709627 1106 84-85 0.6341206415589156 1106 86-87 0.6117025587615892 1106 88-89 0.4022164184617445 1106 90-91 0.5535915379541514 1106 92-93 0.5922425004780614 1106 94-95 0.8403450407828821 1106 96-97 1.1421983030375564 1106 98-99 1.2653115219429587 1106 100-101 1.5523985295760419 1107 1 0.23300831526297472 1107 2 0.21583379866369512 1107 3 0.28002808313984673 1107 4 0.3595776574877263 1107 5 0.3056972055037477 1107 6 0.20878979885436166 1107 7 0.19415971074798222 1107 8 0.285546563716899 1107 9 0.42340316989589866 1107 10-11 0.34176821895318454 1107 12-13 0.22908719666271793 1107 14-15 0.22390508414017063 1107 16-17 0.23595050659875483 1107 18-19 0.37400096582256737 1107 20-21 0.36494966558780106 1107 22-23 0.08338301116749136 1107 24-25 0.08721059453184665 1107 26-27 0.045419947664150584 1107 28-29 0.11081211544575353 1107 30-31 0.12238269201879604 1107 32-33 0.15188878835859043 1107 34-35 -0.06621193169434747 1107 36-37 0.05373970142404971 1107 38-39 0.11746909097745828 1107 40-41 0.2969062064857795 1107 42-43 0.06728785985173857 1107 44-45 0.11148183039210124 1107 46-47 -0.06845495165707405 1107 48-49 -0.13859246828021554 1107 50-51 -0.1947163465987387 1107 52-53 -0.21416373800737887 1107 54-55 0.08056681025823309 1107 56-57 0.11975726783465745 1107 58-59 0.22524351839050638 1107 60-61 0.10828575819490283 1107 62-63 0.15217106326257124 1107 64-65 -0.08840483211331929 1107 66-67 -0.03560234951930141 1107 68-69 -0.05380575488171502 1107 70-71 -0.00662791730825063 1107 72-73 0.2301495138792795 1107 74-75 0.07496882816966988 1107 76-77 0.002559980357968783 1107 78-79 0.12672241467280898 1107 80-81 0.20259946635222192 1107 82-83 0.12671121148129671 1107 84-85 0.029512652574812392 1107 86-87 0.27511595679145984 1107 88-89 0.41866649653199417 1107 90-91 0.4120009266019302 1107 92-93 0.5188175319315569 1107 94-95 0.5892463881265151 1107 96-97 0.907832502588672 1107 98-99 0.8878483474685872 1107 100-101 1.1422073223490443 1108 1 0.24327025181144535 1108 2 0.1647155970936467 1108 3 0.38588032948061723 1108 4 0.49029337048120425 1108 5 0.3539485218762124 1108 6 0.37565958099241215 1108 7 0.5171021607180677 1108 8 0.6057951886881767 1108 9 0.1616542244966297 1108 10-11 0.3226952399036449 1108 12-13 0.36263489195654586 1108 14-15 0.7988559049884429 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-9.018098218765477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2699.0 3 997.0 4 1002.0 5 1023.0 6 1202.0 7 1381.0 8 1832.0 9 2196.0 10 2805.0 11 3613.0 12 4894.0 13 5923.0 14 6724.0 15 8252.0 16 10199.0 17 12864.0 18 10390.0 19 7881.0 20 7830.0 21 8418.0 22 9402.0 23 10788.0 24 12323.0 25 13934.0 26 16258.0 27 19576.0 28 23122.0 29 27997.0 30 34350.0 31 41961.0 32 52813.0 33 68523.0 34 90418.0 35 123960.0 36 172374.0 37 228324.0 38 188159.0 39 29505.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.056222943894056 18.579659183976776 14.232341789963984 22.13177608216518 2 14.895782465156781 20.56117377280277 40.854711795727624 23.688331966312823 3 20.27522522629287 25.475082699507873 29.791100292559136 24.458591781640116 4 12.112303035522542 16.272388746625243 38.85822824215599 32.75707997569622 5 13.003348678149203 38.32182077065426 34.363695840780544 14.311134710415985 6 30.265035677879716 36.25615374281899 18.647480422603103 14.831330156698197 7 27.96684001397741 33.25430192790249 21.016951564473974 17.76190649364613 8 25.81707095376979 33.583493388344834 20.427287601071775 20.1721480568136 9 26.355212147659074 17.309686885359945 21.22259219205545 35.11250877492553 10-11 24.481517212541682 26.42158643184641 27.821583823376557 21.27531253223535 12-13 26.597509766801924 24.002999881063726 27.8567327612538 21.54275759088055 14-15 23.05499029954837 25.296090974826242 27.40502052702494 24.243898198600448 16-17 21.34961426382126 28.71657855447531 27.93297414014541 22.000833041558025 18-19 21.11801634943711 27.979492667174316 29.941353991354642 20.96113699203393 20-21 22.5324548602689 26.397686304843006 29.335274844135288 21.734583990752803 22-23 21.68808293307451 26.550402984490933 29.356263729578703 22.40525035285585 24-25 22.01986689559213 26.52235302297762 29.76182480880957 21.69595527262068 26-27 21.99707648546144 26.133454487989887 30.321270543615675 21.548198482932996 28-29 21.18682830951245 27.08735570800641 30.171088513835915 21.554727468645225 30-31 21.41111917737773 26.771363998663407 29.90260707178986 21.914909752169002 32-33 21.30696126154275 26.79806617798383 29.79917181729735 22.095800743176063 34-35 22.203103409863886 26.38775321563413 29.546386425959238 21.862756948542746 36-37 22.1865153707331 26.08097160554831 30.088315209341292 21.6441978143773 38-39 22.018206567035893 26.396678166576738 30.08373314415231 21.501382122235064 40-41 21.769874452197236 26.781240310383716 29.970118951457614 21.478766285961438 42-43 21.4198056773742 27.077786322527846 29.198714416678616 22.30369358341934 44-45 21.707747209018034 26.9542205887871 28.93106328858941 22.406968913605457 46-47 22.040437473586092 27.116252799380682 28.553366958816024 22.289942768217205 48-49 22.114244501338575 27.018822196715536 28.52123273657693 22.345700565368958 50-51 21.87115391603431 27.37384844592534 28.934953685052033 21.820043952988314 52-53 21.999071815785058 27.68421988091594 28.263051356235124 22.053656947063878 54-55 21.440118744665394 27.11840775347685 28.7240912561285 22.717382245729254 56-57 22.121110382582728 27.516925118693074 28.068640539707868 22.293323959016327 58-59 22.486307911432153 27.116102117346312 28.022943777318016 22.374646193903516 60-61 22.02803832082911 26.987634288404767 29.00013350828976 21.984193882476365 62-63 22.14506057027297 27.132598932762452 28.690204876430325 22.032135620534252 64-65 21.81390245405267 27.68787729894637 28.526367676588944 21.971852570412022 66-67 21.765119674921213 27.379861972646523 28.163212428070434 22.691805924361834 68-69 21.505748393108167 27.69075631845312 28.359739958629422 22.44375532980929 70-71 21.933948290803208 27.46133738635439 27.975364309768562 22.629350013073836 72-73 21.91065677098168 26.962528527392028 28.392562197319283 22.73425250430701 74-75 21.53451852928675 28.04433977021883 28.023956211691946 22.397185488802474 76-77 21.860075007931023 27.29635244008867 28.091983285391624 22.751589266588677 78-79 21.84626166322911 27.23069441520009 28.36949252040312 22.553551401167677 80-81 21.91665293994728 27.808279404114224 27.904030678329043 22.37103697760945 82-83 21.849113571262677 27.587161674300436 28.17502010481362 22.38870464962326 84-85 21.725065596841397 27.469622826555355 28.012645783376183 22.792665793227066 86-87 21.912332658450634 27.546865903203965 27.99816573044137 22.542635707904026 88-89 22.0111723458569 27.11357630006 27.846418359838328 23.028832994244777 90-91 21.740643453873957 27.022100211324627 28.300357474374422 22.936898860426993 92-93 21.86694665257257 27.430244482049776 28.292559990772475 22.410248874605184 94-95 21.47682396770576 27.434304059336206 28.205041096626353 22.883830876331686 96-97 20.99029902453014 27.30608517170466 28.49391941056244 23.209696393202762 98-99 21.3084132826167 27.560813341403325 27.805441555506743 23.325331820473235 100-101 21.335117936286828 27.27467741642377 28.168414265972736 23.221790381316662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 95.0 2 153.5 3 221.5 4 194.0 5 193.5 6 292.0 7 535.5 8 893.5 9 1106.5 10 1081.0 11 1025.5 12 1035.0 13 1114.5 14 1241.0 15 1338.0 16 1401.5 17 1511.5 18 1655.0 19 1826.5 20 2054.0 21 2378.0 22 2782.0 23 3314.0 24 3994.0 25 4996.0 26 6455.0 27 8259.0 28 10112.0 29 12291.0 30 15171.5 31 18360.5 32 21634.5 33 24522.5 34 27469.0 35 30069.0 36 32885.5 37 35798.5 38 37325.5 39 38464.5 40 39408.5 41 40849.5 42 42154.5 43 43361.5 44 45424.0 45 47278.0 46 48671.0 47 54156.0 48 70173.0 49 68629.0 50 51209.5 51 43630.5 52 39491.0 53 37237.0 54 34737.0 55 31757.5 56 28470.5 57 25168.5 58 22794.0 59 20241.5 60 17093.5 61 14115.0 62 11443.0 63 8519.5 64 6048.5 65 4641.5 66 3502.5 67 2571.5 68 1868.5 69 1341.0 70 1027.5 71 820.5 72 650.0 73 577.0 74 443.5 75 335.0 76 261.0 77 157.5 78 93.5 79 71.0 80 54.5 81 39.5 82 28.5 83 16.0 84 8.0 85 8.0 86 7.5 87 5.0 88 4.5 89 3.0 90 2.5 91 2.0 92 1.5 93 2.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07504459228667983 2 0.03064979137603344 3 0.014771935534325396 4 0.021091480147940538 5 0.026858064607864357 6 0.03349358645216026 7 0.08025821659291231 8 0.057191878753217046 9 0.07472861505599906 10-11 0.06355092052066727 12-13 0.04190648021903542 14-15 0.04020810260412635 16-17 0.053005180446697005 18-19 0.04822602483265056 20-21 0.018405673687154105 22-23 0.013468529457767272 24-25 0.022987343532025084 26-27 0.024804212608439437 28-29 0.006161555998274764 30-31 8.689373843720821E-4 32-33 0.010308757150959703 34-35 0.011454174612177447 36-37 0.003989212537344559 38-39 0.008452390920710253 40-41 0.004858149921716641 42-43 0.002330332076270584 44-45 5.134629998562304E-4 46-47 7.109487690317035E-4 48-49 0.0011849146150528392 50-51 0.0012639089227230284 52-53 1.1849146150528393E-4 54-55 3.5547438451585177E-4 56-57 0.0034757495374883284 58-59 0.0029227893837970036 60-61 0.005924573075264197 62-63 0.0022908349224354894 64-65 6.319544613615142E-4 66-67 4.3446869218604104E-4 68-69 0.0030412808453022877 70-71 0.002132846307095111 72-73 0.01931410822536128 74-75 0.014850929841995587 76-77 0.02484370976227453 78-79 0.018761148071669956 80-81 0.010624734381640459 82-83 0.0037917267681690857 84-85 0.0029622865376320983 86-87 0.0011849146150528392 88-89 0.0016193833072388802 90-91 0.007030493382646846 92-93 0.010743225843145743 94-95 0.014021489611458599 96-97 0.013034060765581234 98-99 0.02527817845446057 100-101 0.021565445993961672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.535493302942704 #Duplication Level Percentage of deduplicated Percentage of total 1 81.79447055558207 51.96852036201928 2 11.301484509587457 14.360907867444093 3 3.165549222736632 6.033741943239562 4 1.3008364253505817 3.305971359643433 5 0.6548697918496448 2.080373763718129 6 0.3790423659199436 1.4449586220862878 7 0.2563833817015404 1.1402611241758815 8 0.18812756668748867 0.9562222202697473 9 0.13691479269567927 0.7829054004951106 >10 0.748163904365118 8.606747742534692 >50 0.04797601451534822 2.0987631521484325 >100 0.021443891792702927 2.443054421136122 >500 0.001994769354045768 0.8078797135505908 >1k 0.0026181347771850705 3.503927750653043 >5k 1.246730846278605E-4 0.4657645568857216 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5880 0.464486529100713 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4335 0.34244032375027056 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4049 0.3198479517565964 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3695 0.29188396684134943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.27505817930759907 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3209 0.2534927333136374 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2682 0.21186273317144766 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2644 0.2088609494799805 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2452 0.19369404240730412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2093 0.1653350859537062 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1746 0.13792406119215048 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1718 0.1357122205773852 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1419 0.1120929225839986 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1396 0.11027605350758425 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1363 0.10766924135446801 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1297 0.1024556170482355 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1282 0.10127070243318266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5798861534037858E-4 0.0 16-17 0.0 0.0 0.0 1.5798861534037858E-4 0.0 18-19 0.0 0.0 0.0 1.5798861534037858E-4 0.0 20-21 0.0 0.0 0.0 1.9748576917547322E-4 0.0 22-23 0.0 0.0 0.0 2.3698292301056785E-4 3.9497153835094645E-5 24-25 0.0 0.0 0.0 6.319544613615143E-4 7.899430767018929E-5 26-27 0.0 0.0 0.0 0.0010269259997124607 7.899430767018929E-5 28-29 0.0 0.0 0.0 0.001224411768887934 7.899430767018929E-5 30-31 0.0 0.0 0.0 0.002409326383940773 7.899430767018929E-5 32-33 0.0 0.0 0.0 0.00501613853705702 7.899430767018929E-5 34-35 0.0 0.0 0.0 0.007109487690317036 7.899430767018929E-5 36-37 0.0 0.0 0.0 0.009360825458917432 7.899430767018929E-5 38-39 0.0 0.0 0.0 0.011770151842858203 7.899430767018929E-5 40-41 0.0 0.0 0.0 0.015601375764862386 7.899430767018929E-5 42-43 0.0 0.0 0.0 0.02235538907066357 7.899430767018929E-5 44-45 0.0 0.0 0.0 0.030570797068363256 7.899430767018929E-5 46-47 0.0 0.0 0.0 0.05399260929257438 7.899430767018929E-5 48-49 0.0 0.0 0.0 0.09767646143418905 7.899430767018929E-5 50-51 0.0 0.0 0.0 0.12516648050341492 7.899430767018929E-5 52-53 0.0 0.0 0.0 0.14997069311185435 1.1849146150528394E-4 54-55 0.0 0.0 0.0 0.18954684125461918 1.5798861534037858E-4 56-57 0.0 0.0 0.0 0.22011763832298245 1.5798861534037858E-4 58-59 0.0 0.0 0.0 0.2589828376967156 1.5798861534037858E-4 60-61 0.0 0.0 0.0 0.3267994508315731 1.5798861534037858E-4 62-63 0.0 0.0 0.0 0.37376156674150063 1.5798861534037858E-4 64-65 0.0 0.0 0.0 0.42779367318791006 1.5798861534037858E-4 66-67 0.0 0.0 0.0 0.5315526963127037 1.5798861534037858E-4 68-69 0.0 0.0 0.0 0.651821529740567 1.5798861534037858E-4 70-71 0.0 0.0 0.0 0.7469701733293099 1.5798861534037858E-4 72-73 0.0 0.0 0.0 0.8506502021464333 1.5798861534037858E-4 74-75 0.0 0.0 0.0 0.9371094718914554 1.5798861534037858E-4 76-77 0.0 0.0 0.0 1.0008973753351333 1.5798861534037858E-4 78-79 0.0 0.0 0.0 1.0885020625413733 1.5798861534037858E-4 80-81 0.0 0.0 0.0 1.2389072243454136 1.9748576917547322E-4 82-83 0.0 0.0 0.0 1.411904758143128 2.3698292301056785E-4 84-85 0.0 0.0 0.0 1.6162630320859077 2.3698292301056785E-4 86-87 0.0 0.0 0.0 1.9790443900612522 2.3698292301056785E-4 88-89 0.0 0.0 0.0 2.4291144580121555 2.3698292301056785E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1135 0.0 30.147367 2 GTACCTG 1315 0.0 27.105999 1 GTACATG 7540 0.0 26.410255 1 TACATGG 7590 0.0 25.922169 2 ACATGGG 7480 0.0 24.973095 3 CATGGGA 5175 0.0 21.677053 4 CATGGGG 2260 0.0 21.663437 4 GTATAGA 395 0.0 21.65735 1 CATACCG 110 0.0028691108 21.601809 2 GAGTACT 4795 0.0 21.264465 12-13 GTGTAAG 555 0.0 19.6954 1 GTACTTT 5230 0.0 19.535467 14-15 AGTACTT 5005 0.0 19.185057 12-13 ACACCGC 175 9.857393E-5 19.020117 8 GTGTACG 125 0.006012546 19.010342 1 ATGGGAG 1725 0.0 18.739273 5 ATGGGAT 2025 0.0 18.54535 5 ACCTGGG 1840 0.0 17.82431 3 GTGTAGC 830 0.0 17.75062 1 ATGGGGG 1355 0.0 17.541376 5 >>END_MODULE