FastQCFastQC Report
Thu 26 May 2016
SRR936536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265914
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77590.6129168332129986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.4396823164922735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.3955244985046378No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40810.32237576960204245No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40440.3194529802182455No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28910.22837254347451721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.2278195833208259No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26970.2130476477865005No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21230.16770491518381186No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21050.16628301764574843No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18140.14329567411372335No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17050.13468529457767273No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16570.13089356780950365No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16360.1292346873484297No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.11525269489080618No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14210.11225091119933897No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14020.11075001935360537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13050.028.7532712
GTACCTG14200.026.7665521
TAAGGTG4950.023.9838945
GTACATG73200.021.1591051
GAGTACT59050.021.02987512-13
AGTACTT60500.020.83982312-13
TACATGG74050.020.4614452
ACATGGG71050.020.4555363
GTACTTT63600.019.5253814-15
ACGATCG757.703728E-419.00763182-83
ATGGGAG18750.018.7419745
ACCTGGG18900.018.5961673
TGGACCG1559.641454E-418.3824945
AACGCGA650.00756184918.2707976-77
TAACGCA1300.007574467518.2646564
CATGGGA49950.018.1586174
GTAAGGT4550.017.7428114
TACTTTT72750.017.26543814-15
CATTCCG1952.2798973E-417.0470149
CATGGGG23700.017.03164
Produced by FastQC (version 0.11.5)