##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936536_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265914 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03943000867358 31.0 31.0 34.0 31.0 34.0 2 32.20032008493468 33.0 31.0 34.0 30.0 34.0 3 32.281693701151895 34.0 31.0 34.0 30.0 34.0 4 35.95155832070741 37.0 35.0 37.0 35.0 37.0 5 35.81328431473228 37.0 35.0 37.0 35.0 37.0 6 35.91413081773327 37.0 35.0 37.0 35.0 37.0 7 35.86642141567279 37.0 35.0 37.0 35.0 37.0 8 35.847420914848875 37.0 35.0 37.0 35.0 37.0 9 37.50080574193824 39.0 37.0 39.0 35.0 39.0 10-11 37.393105297832236 39.0 37.0 39.0 34.0 39.0 12-13 37.29961197996072 39.0 37.0 39.0 34.0 39.0 14-15 38.433262449107914 40.0 38.0 41.0 34.0 41.0 16-17 38.35250419854745 40.0 38.0 41.0 33.5 41.0 18-19 38.5269026963917 40.0 38.0 41.0 34.0 41.0 20-21 38.553942447907204 40.0 38.0 41.0 34.0 41.0 22-23 38.47750676586246 40.0 38.0 41.0 34.0 41.0 24-25 38.346929175283634 40.0 38.0 41.0 34.0 41.0 26-27 38.15181836996826 40.0 37.5 41.0 33.5 41.0 28-29 38.076218447698665 40.0 37.5 41.0 33.5 41.0 30-31 38.06624265155453 40.0 37.0 41.0 34.0 41.0 32-33 37.90652722064848 40.0 37.0 41.0 33.0 41.0 34-35 37.733844479166834 40.0 37.0 41.0 33.0 41.0 36-37 37.52818635389134 40.0 36.5 41.0 33.0 41.0 38-39 37.362318846303936 40.0 36.0 41.0 32.5 41.0 40-41 37.18047355507562 39.0 36.0 41.0 32.0 41.0 42-43 36.91329782275889 39.0 36.0 41.0 31.0 41.0 44-45 36.61609279935288 39.0 36.0 41.0 30.5 41.0 46-47 36.20785851171564 39.0 35.0 40.5 30.0 41.0 48-49 35.810763211402985 39.0 35.0 40.0 28.5 41.0 50-51 35.524176602834004 39.0 35.0 40.0 28.0 41.0 52-53 35.16448313234548 38.0 35.0 40.0 27.0 41.0 54-55 35.010458451363995 38.0 34.0 40.0 26.5 41.0 56-57 34.7309738260261 38.0 34.0 40.0 26.0 41.0 58-59 34.39080300873519 38.0 34.0 40.0 25.5 41.0 60-61 33.968254557576586 37.5 33.0 40.0 24.0 41.0 62-63 33.664351211851674 37.0 33.0 39.5 23.0 41.0 64-65 33.24736474989612 36.0 32.0 39.0 22.5 40.0 66-67 32.83021555966677 36.0 32.0 39.0 22.0 40.0 68-69 32.481230557526025 35.0 31.5 38.5 21.0 40.0 70-71 32.11062046868903 35.0 31.0 38.0 20.0 40.0 72-73 31.646656091962015 35.0 31.0 37.0 18.5 39.5 74-75 31.147786500504772 34.5 30.5 37.0 18.0 39.0 76-77 29.6461987149206 33.0 28.5 35.0 14.0 37.5 78-79 30.392270722971702 34.0 30.0 36.0 14.0 38.0 80-81 30.142700057033892 34.0 30.0 35.5 9.0 37.0 82-83 29.844724049185015 34.0 29.5 35.0 7.5 37.0 84-85 29.483769039603004 34.0 29.0 35.0 6.0 36.0 86-87 29.118797564447505 34.0 29.0 35.0 2.0 36.0 88-89 28.64334228075525 34.0 29.0 35.0 2.0 35.5 90-91 28.162979080727442 33.0 28.0 35.0 2.0 35.0 92-93 27.568511368070816 33.0 26.5 35.0 2.0 35.0 94-95 27.080448987845937 33.0 26.0 34.5 2.0 35.0 96-97 26.40066347319012 32.0 25.0 34.0 2.0 35.0 98-99 25.582424240509226 32.0 23.5 34.0 2.0 35.0 100-101 22.40634948345622 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10386546598716251 1101 2 0.11768243445928306 1101 3 0.021700614309736466 1101 4 0.03749134886235339 1101 5 0.034430544117178385 1101 6 0.06308069374604486 1101 7 0.1314141703246321 1101 8 0.07561207002959236 1101 9 0.04611659055760242 1101 10-11 0.019781370286004574 1101 12-13 0.0689193179050207 1101 14-15 0.26117571311072396 1101 16-17 0.2801913495391517 1101 18-19 0.1608556185120733 1101 20-21 0.17972134806966267 1101 22-23 0.04475561990734178 1101 24-25 -0.0019428677780553016 1101 26-27 0.07511130665318433 1101 28-29 -0.07385278997684708 1101 30-31 0.008089113349086574 1101 32-33 -0.1025716269508834 1101 34-35 -0.13362842350184678 1101 36-37 -0.07746744648515147 1101 38-39 -0.17826011143828424 1101 40-41 -0.09335323257133155 1101 42-43 -0.033796818434410625 1101 44-45 0.03526410946357572 1101 46-47 -0.08420737961967717 1101 48-49 -0.11900855384922693 1101 50-51 -0.08739826781576454 1101 52-53 -0.07723323371744328 1101 54-55 -0.15629067352114134 1101 56-57 -0.16650699183748685 1101 58-59 -0.24986594904587633 1101 60-61 -0.13482140627633044 1101 62-63 -0.3657974499061538 1101 64-65 -0.4008513815809067 1101 66-67 -0.5901383333424093 1101 68-69 -0.65827957613417 1101 70-71 -0.4751897288554474 1101 72-73 -0.2694067256879471 1101 74-75 -0.29364126074592534 1101 76-77 -0.49344627329450574 1101 78-79 -0.3396588040163202 1101 80-81 -0.09719101298477639 1101 82-83 -0.22885979446136062 1101 84-85 -0.2332251065725508 1101 86-87 -0.33772463980811196 1101 88-89 -0.5025740818035054 1101 90-91 -0.4897044201091241 1101 92-93 -0.4227068677599064 1101 94-95 -0.4724529472604395 1101 96-97 -0.2509629452665294 1101 98-99 -0.4750853064582934 1101 100-101 0.15161984371225046 1102 1 0.22713422339871414 1102 2 0.1410520950376295 1102 3 0.20970301577803951 1102 4 0.20255711183598635 1102 5 0.18117090272261294 1102 6 0.248766878131363 1102 7 0.20015417078471387 1102 8 0.19697192175561185 1102 9 0.24598379711861185 1102 10-11 0.25120325693615797 1102 12-13 0.2955389445407519 1102 14-15 0.4198929663794786 1102 16-17 0.38449148436237834 1102 18-19 0.24601482404285235 1102 20-21 0.21155072654313756 1102 22-23 0.16837778648822876 1102 24-25 0.23105706862182984 1102 26-27 0.16441196245610712 1102 28-29 0.21725397387320555 1102 30-31 0.3415585178580187 1102 32-33 0.3282634696694231 1102 34-35 0.2619433393085018 1102 36-37 0.3074296453655947 1102 38-39 0.25432872462091183 1102 40-41 0.32559585891676335 1102 42-43 0.3893692766581438 1102 44-45 0.4822358191385163 1102 46-47 0.5492195797037525 1102 48-49 0.7127528942848755 1102 50-51 0.684933831254277 1102 52-53 0.7636000270696499 1102 54-55 0.6552664603598188 1102 56-57 0.38201899647902593 1102 58-59 0.5575208664240066 1102 60-61 0.6388692995812377 1102 62-63 0.6283150284822128 1102 64-65 0.4239544238018311 1102 66-67 0.32395369377379524 1102 68-69 0.3935915077787442 1102 70-71 0.4117324178334911 1102 72-73 0.6082313824205237 1102 74-75 0.4992483362142579 1102 76-77 0.3835799655884422 1102 78-79 0.44752392273275987 1102 80-81 0.6277215594054617 1102 82-83 0.35889551378044615 1102 84-85 0.4034880844077797 1102 86-87 0.41776064488361087 1102 88-89 0.458160513097571 1102 90-91 0.4901670445023747 1102 92-93 0.7864937689629805 1102 94-95 0.46736307248176345 1102 96-97 0.5614421901949562 1102 98-99 0.6892634892215916 1102 100-101 1.349633625542193 1103 1 0.13315436075180997 1103 2 0.18823431543770397 1103 3 0.08055527688295427 1103 4 0.08377677648208248 1103 5 0.13752391042600465 1103 6 0.09287080884421783 1103 7 0.04352968069023433 1103 8 0.05191850349971361 1103 9 0.18692389359604533 1103 10-11 0.20441941219710458 1103 12-13 0.13808616596359258 1103 14-15 0.22710608443664881 1103 16-17 0.18125419482365146 1103 18-19 0.20217313358095623 1103 20-21 0.09354283261662744 1103 22-23 0.13226664252591291 1103 24-25 0.2378732564711683 1103 26-27 0.13953636998455465 1103 28-29 0.05725216048646331 1103 30-31 0.08127707498035619 1103 32-33 0.18802866674820962 1103 34-35 0.2163371009077153 1103 36-37 0.15933245309273047 1103 38-39 0.12110152259341334 1103 40-41 0.3303557800913808 1103 42-43 0.33979461221134244 1103 44-45 0.3056947360735691 1103 46-47 0.35558141343195615 1103 48-49 0.3113238228921915 1103 50-51 0.4004907621804463 1103 52-53 0.39581059368291704 1103 54-55 0.32958702070507684 1103 56-57 0.28924718229072965 1103 58-59 0.30610202270772646 1103 60-61 0.2509891618164133 1103 62-63 0.4178198118372478 1103 64-65 0.3534625185587359 1103 66-67 0.1810180834574595 1103 68-69 0.3436318271625751 1103 70-71 0.31967895008177294 1103 72-73 0.35290127376186575 1103 74-75 0.20607385380695575 1103 76-77 0.24439946746774766 1103 78-79 0.0884777291147465 1103 80-81 0.2967966577072296 1103 82-83 0.3459186292051406 1103 84-85 0.7540937073959206 1103 86-87 0.5803465343695144 1103 88-89 0.48758468396590615 1103 90-91 0.3960240208508168 1103 92-93 0.31925146151158046 1103 94-95 0.2794429876467994 1103 96-97 0.5236503783807009 1103 98-99 0.44536866740435954 1103 100-101 0.9795187645750794 1104 1 0.20924374396788892 1104 2 0.1608107586023806 1104 3 0.17384634981203817 1104 4 0.15554255469582756 1104 5 0.09751232573115942 1104 6 0.08819166445918825 1104 7 0.11680287556976054 1104 8 0.13892149548008348 1104 9 0.21971877593843914 1104 10-11 0.22290416399633273 1104 12-13 0.19284604283885187 1104 14-15 0.1743596855364089 1104 16-17 0.25717262028958743 1104 18-19 0.12321790077957218 1104 20-21 0.1268231207046071 1104 22-23 0.21824828280619357 1104 24-25 0.20551104226506567 1104 26-27 0.2166071122136728 1104 28-29 0.28073371287073456 1104 30-31 0.3014328618782329 1104 32-33 0.17490403371407126 1104 34-35 0.07199799983784771 1104 36-37 0.08253924253255462 1104 38-39 0.1470657858314368 1104 40-41 0.32856380007542185 1104 42-43 0.30471069788534777 1104 44-45 0.3280778959001722 1104 46-47 0.21675361227384116 1104 48-49 0.3741121218230248 1104 50-51 0.28438869556932644 1104 52-53 0.3585879495598263 1104 54-55 0.19777288210360666 1104 56-57 0.09978402478458293 1104 58-59 0.14273277506246274 1104 60-61 0.35702867283330164 1104 62-63 0.1943516635953486 1104 64-65 0.3224701637541614 1104 66-67 0.2702112244039938 1104 68-69 0.15879683435246505 1104 70-71 0.2767079065451128 1104 72-73 0.3893739451327285 1104 74-75 0.17044124860421306 1104 76-77 -0.07369049937787508 1104 78-79 0.011379079900709854 1104 80-81 0.3116849242923294 1104 82-83 0.3724713562669173 1104 84-85 0.3665109532973254 1104 86-87 0.36724374349523004 1104 88-89 0.3968713814313354 1104 90-91 0.5845339460688841 1104 92-93 0.8073285867493389 1104 94-95 0.8741689630288825 1104 96-97 1.0196609979772937 1104 98-99 0.8326398301280129 1104 100-101 1.2909679677098005 1105 1 -0.03338546574488177 1105 2 0.04020788703744671 1105 3 0.04776380124460644 1105 4 0.09935266157630451 1105 5 0.13638322668287373 1105 6 -0.025286734546973832 1105 7 -0.04271373481270757 1105 8 0.026140104248995044 1105 9 -0.10633628499842729 1105 10-11 0.08491402741494625 1105 12-13 0.1478238920924042 1105 14-15 0.24566688301182893 1105 16-17 0.1599378045281057 1105 18-19 0.10601314292060948 1105 20-21 -0.008577109905900215 1105 22-23 0.16695172340077846 1105 24-25 0.07062308113328442 1105 26-27 -0.007850810292630683 1105 28-29 0.03459670666638459 1105 30-31 0.001226883140084567 1105 32-33 0.13743875023449448 1105 34-35 0.16241400906226744 1105 36-37 0.11780239197244669 1105 38-39 0.09800081149845852 1105 40-41 0.20607918600889263 1105 42-43 0.25072472371657994 1105 44-45 0.39193398208995944 1105 46-47 0.15214250869902202 1105 48-49 0.08287041273496953 1105 50-51 0.03271132538591104 1105 52-53 0.34855407194761057 1105 54-55 0.10843986767439162 1105 56-57 -0.03242299991144648 1105 58-59 0.013920205004701813 1105 60-61 0.010177612953100379 1105 62-63 -0.16589426145267083 1105 64-65 -0.07577339450176623 1105 66-67 -0.11505781524245151 1105 68-69 0.008777289576194391 1105 70-71 0.18009156247137525 1105 72-73 -0.06923619815993476 1105 74-75 0.07726085835125929 1105 76-77 -0.16050946655692755 1105 78-79 -0.07489504317247864 1105 80-81 -0.005973022488763746 1105 82-83 0.3051476340847543 1105 84-85 0.39982274870368073 1105 86-87 0.3740063253334043 1105 88-89 0.23265108413654545 1105 90-91 0.2591281465358186 1105 92-93 0.19904472520132188 1105 94-95 0.45070684241173353 1105 96-97 0.5128421914402317 1105 98-99 0.24873919255777466 1105 100-101 0.5401821606087118 1106 1 0.016093821794150642 1106 2 0.10328858632019688 1106 3 -0.020673733737275768 1106 4 0.04000108806269509 1106 5 0.0897994153206696 1106 6 -0.0959499137188331 1106 7 -0.10926372694036246 1106 8 0.04159675037475097 1106 9 0.10502346483388436 1106 10-11 0.05932799268378375 1106 12-13 0.11287067567023712 1106 14-15 0.171385154954514 1106 16-17 0.0027673259167713127 1106 18-19 0.04172815671313401 1106 20-21 -0.0853252030234728 1106 22-23 -0.11374724081689891 1106 24-25 -0.001100134460692459 1106 26-27 -0.15510349803899004 1106 28-29 -0.18835837181590165 1106 30-31 -0.08658754427642634 1106 32-33 -0.043716673538895634 1106 34-35 -0.03735860300035654 1106 36-37 -0.4031123382336119 1106 38-39 0.009704032243590177 1106 40-41 0.033225219393102634 1106 42-43 -0.004846667540839178 1106 44-45 -0.01668559975650652 1106 46-47 0.13796546009161403 1106 48-49 -0.002124304147251621 1106 50-51 0.05905410644459863 1106 52-53 -0.11622741811913073 1106 54-55 0.06665659472604091 1106 56-57 -0.1613483655204888 1106 58-59 -0.055673609242518296 1106 60-61 -0.21491960824526046 1106 62-63 -0.03236835142311634 1106 64-65 -0.15810357252599516 1106 66-67 -0.03556327446415253 1106 68-69 -0.2697481335510332 1106 70-71 -0.26889290488932716 1106 72-73 -0.10723755966625603 1106 74-75 -0.1588216984411197 1106 76-77 -0.17164906372943634 1106 78-79 -0.36543739775932593 1106 80-81 -0.2494273715276556 1106 82-83 -0.07838851454513929 1106 84-85 0.12430542635705066 1106 86-87 0.16690970115920223 1106 88-89 -0.060164012201855854 1106 90-91 -0.006373094382542632 1106 92-93 0.08802644406665294 1106 94-95 -0.10724158780606174 1106 96-97 0.2884826045154263 1106 98-99 0.3380288885214 1106 100-101 0.7712464380866138 1107 1 0.012697800030103679 1107 2 0.050368840898883604 1107 3 -0.08696582712651235 1107 4 5.083329546593518E-4 1107 5 -0.05605193291089705 1107 6 -0.10285397405491636 1107 7 -0.08625520136843079 1107 8 -0.04208936760080206 1107 9 -0.06399213965389094 1107 10-11 0.0013913969292289607 1107 12-13 -0.1347902904337559 1107 14-15 0.02032434212230072 1107 16-17 0.01563696430739725 1107 18-19 -0.1414022486101203 1107 20-21 -0.0887178096409329 1107 22-23 -0.060423142039930156 1107 24-25 -0.05402875926312589 1107 26-27 -0.11897671140504684 1107 28-29 -0.001401636258897554 1107 30-31 -0.12766151225327604 1107 32-33 -0.12273223704491443 1107 34-35 -0.1406128278781935 1107 36-37 -0.1817145323969882 1107 38-39 -0.14718916792634928 1107 40-41 -0.23622643101063545 1107 42-43 -0.2054332531182652 1107 44-45 -0.2249760730001995 1107 46-47 -0.329267574484696 1107 48-49 -0.4135565756432058 1107 50-51 -0.5547939031396751 1107 52-53 -0.30007295513726717 1107 54-55 -0.36145252057340116 1107 56-57 -0.5373389287874488 1107 58-59 -0.4447626257212818 1107 60-61 -0.43875719480215736 1107 62-63 -0.5687239528782371 1107 64-65 -0.3635915764821007 1107 66-67 -0.27024736657475046 1107 68-69 -0.23039130127708063 1107 70-71 -0.25860648337554437 1107 72-73 -0.11530140378257059 1107 74-75 -0.20350137818233094 1107 76-77 -0.19662129858770427 1107 78-79 -0.20530049136302964 1107 80-81 -0.07236704774443581 1107 82-83 -0.3110125902357126 1107 84-85 -0.12099703951539098 1107 86-87 -0.20819702217157499 1107 88-89 -0.32323616878496253 1107 90-91 0.10174908043744679 1107 92-93 -0.011545569763324437 1107 94-95 0.1173732861135548 1107 96-97 0.10091865589562232 1107 98-99 0.0234604196135777 1107 100-101 0.5651238998950276 1108 1 0.10478424675034859 1108 2 0.16090001451849645 1108 3 0.06385201562236631 1108 4 0.09253466912967667 1108 5 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35.0 6 68.0 7 173.0 8 353.0 9 710.0 10 1221.0 11 2249.0 12 3268.0 13 4714.0 14 5845.0 15 8285.0 16 11053.0 17 14683.0 18 12549.0 19 7711.0 20 6787.0 21 6774.0 22 7277.0 23 7938.0 24 9721.0 25 11418.0 26 13713.0 27 17401.0 28 21653.0 29 27225.0 30 33974.0 31 42761.0 32 55024.0 33 71429.0 34 96099.0 35 132302.0 36 192270.0 37 258024.0 38 170265.0 39 10922.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55536759634051 19.46815882228847 14.717522583418205 21.258950997952816 2 15.452608393028274 21.730512008380863 39.6988009395287 23.11807865906216 3 20.698985468547313 25.74020031505618 30.056185545020327 23.50462867137618 4 12.733566419203832 17.070985864758587 38.21349633545407 31.981951380583517 5 13.019157530020783 38.79149014158608 34.01984243285069 14.169509895542443 6 30.408068794562666 37.01531067671264 17.74472831487763 14.83189221384707 7 28.02014986800051 33.87102125420842 20.572487546547396 17.536341331243673 8 26.27137388479786 34.17001470874009 19.655126651573486 19.903484754888563 9 26.69170259591094 17.56920296323447 20.68118371390158 35.05791072695302 10-11 24.76720377529595 26.945432312147588 27.191934049232415 21.09542986332405 12-13 27.087117688763552 24.353613331200467 27.624125870673293 20.935143109362688 14-15 23.353449273507664 25.725060559130714 27.212170835007026 23.709319332354593 16-17 21.31369955372148 29.10064550224008 27.89906261367779 21.686592330360643 18-19 21.120925450561916 28.055366514428766 30.306546809106464 20.517161225902854 20-21 22.50322691746833 26.414630061757748 29.982921430681703 21.099221590092217 22-23 21.820417358854836 26.503282611048313 29.861056802481688 21.81524322761517 24-25 22.212488368088195 26.570841305175545 30.201498680005116 21.015171646731137 26-27 22.16886076649327 26.163662863033665 30.80962481090454 20.857851559568527 28-29 21.2747477237071 27.128726871710775 30.71502377013826 20.881501634443868 30-31 21.506497569114064 26.81317153495566 30.322463171357217 21.357867724573058 32-33 21.503860021678058 26.93878002458532 30.184958713726385 21.372401240010237 34-35 22.249655774962537 26.364715018007136 30.154310771814547 21.231318435215783 36-37 22.347762822269953 26.13699469864595 30.524179182765188 20.991063296318913 38-39 22.08861514447022 26.387332026754066 30.632253233072724 20.89179959570298 40-41 21.80813730512544 26.825000661579157 30.358544304672407 21.008317728622995 42-43 21.585880984864584 27.29588833967037 29.547833492252963 21.570397183212084 44-45 21.985168508260013 26.90476049411974 29.20381945473038 21.90625154288987 46-47 22.400794371777998 27.382562370349774 28.587154180484177 21.629489077388055 48-49 22.67247014493555 27.30179732721388 28.439637177281913 21.586095350568662 50-51 22.24014515999711 27.60966685322496 28.83261771045178 21.317570276326148 52-53 22.473788670227997 28.030057369683924 28.143690815913423 21.352463144174656 54-55 21.73845210150483 27.537176398748546 28.549072767352314 22.17529873239431 56-57 22.477167145972253 27.814129507315993 27.930505957083717 21.778197389628037 58-59 22.909597321402114 27.372702401058795 27.82984187185793 21.887858405681158 60-61 22.3338953642719 27.343147541812574 28.87196914149906 21.45098795241647 62-63 22.57430177837594 27.303693866046842 28.63805848545527 21.48394587012195 64-65 22.15225477771422 28.037407914239754 28.353762598090494 21.456574709955532 66-67 22.140706459121407 27.674263642622325 28.06237417315196 22.122655725104305 68-69 21.98074816801096 27.8911814507481 28.14199006165942 21.986080319581518 70-71 22.435428027044512 27.881773865976573 27.757239241559255 21.92555886541966 72-73 22.469149748383284 27.28199833558928 28.123130055884545 22.125721860142892 74-75 22.056239918224062 28.45774304090851 27.629319265849222 21.85669777501821 76-77 22.418426634330924 27.663887319938222 27.761803007382184 22.155883038348666 78-79 22.35484371735018 27.588097483053204 27.97232105084573 22.08473774875089 80-81 22.513430267491348 28.112248949103048 27.554891366823774 21.81942941658183 82-83 22.358420203495033 27.967767575109704 27.741604921299345 21.932207300095914 84-85 22.1942675989674 27.82102228055467 27.668004959136116 22.31670516134181 86-87 22.245050082816284 27.948609267128237 27.49350485698835 22.312835793067133 88-89 22.384320642714243 27.62636781655522 27.380948147340312 22.608363393390224 90-91 22.19686180663456 27.440619482594837 27.736356351071752 22.626162359698856 92-93 22.31424778551279 27.893293718837164 27.64185756165401 22.150600933996035 94-95 21.975915819550785 27.85385764502246 27.57290583037243 22.597320705054326 96-97 21.577590243844607 27.655230872889007 27.974574245061756 22.79260463820463 98-99 21.89827491704166 27.944028165329442 27.162874181926245 22.994822735702652 100-101 21.728647021231946 28.045944122632537 27.160038114193192 23.065370741942328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 247.0 2 232.5 3 271.5 4 241.5 5 241.0 6 342.5 7 625.5 8 984.5 9 1210.5 10 1270.5 11 1230.0 12 1227.5 13 1319.0 14 1459.5 15 1664.0 16 1850.0 17 1929.0 18 2056.5 19 2317.0 20 2514.0 21 2765.5 22 3184.0 23 3712.0 24 4420.5 25 5356.5 26 6568.5 27 8344.0 28 10330.0 29 12631.0 30 15541.5 31 18681.5 32 21852.5 33 24593.0 34 27451.5 35 29961.0 36 32853.5 37 35821.5 38 37113.0 39 38031.0 40 39166.0 41 40978.0 42 42069.0 43 42804.0 44 44481.0 45 45952.5 46 47474.5 47 53613.0 48 71808.5 49 69553.0 50 49999.5 51 42957.5 52 38879.0 53 36529.5 54 34250.0 55 31371.5 56 28166.5 57 25039.5 58 22792.5 59 20390.0 60 17151.0 61 13865.0 62 11130.5 63 8453.5 64 5985.5 65 4460.5 66 3348.0 67 2592.5 68 1917.5 69 1301.0 70 1002.0 71 803.0 72 639.0 73 581.5 74 499.0 75 398.0 76 295.0 77 185.5 78 123.5 79 86.5 80 59.5 81 46.5 82 44.5 83 33.0 84 13.5 85 6.0 86 4.5 87 2.5 88 2.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021565445993961672 2 0.013666015226942746 3 0.008531385228380444 4 0.0 5 0.0032387666144777605 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.084345382071768E-4 14-15 1.9748576917547322E-4 16-17 3.949715383509464E-5 18-19 0.0017773719225792587 20-21 0.0 22-23 7.899430767018928E-5 24-25 0.0 26-27 0.0015008918457335963 28-29 0.0031597723068075715 30-31 0.0013824003842283126 32-33 0.009321328305082336 34-35 0.002132846307095111 36-37 8.689373843720821E-4 38-39 0.0010269259997124607 40-41 2.764800768456625E-4 42-43 0.005924573075264197 44-45 0.002093349153260016 46-47 6.319544613615142E-4 48-49 5.134629998562304E-4 50-51 3.949715383509464E-5 52-53 1.1849146150528393E-4 54-55 0.0018563662302494483 56-57 0.01532489568801672 58-59 0.025791641454316803 60-61 0.02101248584027035 62-63 0.014258472534469166 64-65 0.019432599686866565 66-67 0.0029622865376320983 68-69 6.714516151966089E-4 70-71 7.899430767018928E-5 72-73 3.949715383509464E-5 74-75 0.0012639089227230284 76-77 0.0022908349224354894 78-79 0.009044848228236674 80-81 0.016549307456904656 82-83 0.018326679379483915 84-85 0.029306888145640222 86-87 0.012678586381065381 88-89 0.04190648021903542 90-91 0.04739658460211357 92-93 0.030333814145352685 94-95 0.031163254375889675 96-97 0.052254734523830206 98-99 0.052965683292861915 100-101 0.06070712544454047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.82612489963451 #Duplication Level Percentage of deduplicated Percentage of total 1 80.26649882764067 48.823000829464384 2 12.159580864018007 14.792403687239297 3 3.4727539970567114 6.337025051120294 4 1.417483461444932 3.4488010427606275 5 0.7170170282136028 2.1806683656642676 6 0.4338568062310621 1.583389697062026 7 0.30166965107173466 1.2844577112162945 8 0.2003682871919856 0.975010117013244 9 0.14890123862725815 0.8151376804606697 >10 0.797080109370388 8.991003086749384 >50 0.05114449685587742 2.1329340813183775 >100 0.0285789782271803 3.2552894673313006 >500 0.0019485592502336243 0.780752539732886 >1k 0.002727982950327074 3.151613784008288 >5k 3.897118500467249E-4 1.448512858858746 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7759 0.6129168332129986 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5566 0.4396823164922735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5007 0.3955244985046378 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4081 0.32237576960204245 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4044 0.3194529802182455 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2891 0.22837254347451721 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2884 0.2278195833208259 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2697 0.2130476477865005 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2123 0.16770491518381186 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2105 0.16628301764574843 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1814 0.14329567411372335 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1705 0.13468529457767273 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1657 0.13089356780950365 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1636 0.1292346873484297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1459 0.11525269489080618 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1421 0.11225091119933897 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1402 0.11075001935360537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.899430767018929E-5 2 0.0 0.0 0.0 0.0 7.899430767018929E-5 3 0.0 0.0 0.0 0.0 7.899430767018929E-5 4 0.0 0.0 0.0 0.0 7.899430767018929E-5 5 0.0 0.0 0.0 0.0 7.899430767018929E-5 6 0.0 0.0 0.0 0.0 7.899430767018929E-5 7 0.0 0.0 0.0 0.0 7.899430767018929E-5 8 0.0 0.0 0.0 0.0 1.5798861534037858E-4 9 0.0 0.0 0.0 0.0 1.5798861534037858E-4 10-11 0.0 0.0 0.0 3.9497153835094645E-5 1.5798861534037858E-4 12-13 0.0 0.0 0.0 7.899430767018929E-5 1.5798861534037858E-4 14-15 0.0 0.0 0.0 7.899430767018929E-5 1.5798861534037858E-4 16-17 0.0 0.0 0.0 7.899430767018929E-5 2.3698292301056785E-4 18-19 0.0 0.0 0.0 7.899430767018929E-5 2.3698292301056785E-4 20-21 0.0 0.0 0.0 1.5798861534037858E-4 2.764800768456625E-4 22-23 0.0 0.0 0.0 2.3698292301056785E-4 3.554743845158518E-4 24-25 0.0 0.0 0.0 6.319544613615143E-4 4.739658460211357E-4 26-27 0.0 0.0 0.0 0.0010269259997124607 4.739658460211357E-4 28-29 0.0 0.0 0.0 0.001224411768887934 4.739658460211357E-4 30-31 0.0 0.0 0.0 0.002527817845446057 4.739658460211357E-4 32-33 0.0 0.0 0.0 0.0052136243062324925 4.739658460211357E-4 34-35 0.0 0.0 0.0 0.007385967767162698 5.52960153691325E-4 36-37 0.0 0.0 0.0 0.009755796997268376 6.319544613615143E-4 38-39 0.0 0.0 0.0 0.012204620535044244 6.319544613615143E-4 40-41 0.0 0.0 0.0 0.016114838764718614 6.319544613615143E-4 42-43 0.0 0.0 0.0 0.02290834922435489 6.319544613615143E-4 44-45 0.0 0.0 0.0 0.03124224868355986 6.319544613615143E-4 46-47 0.0 0.0 0.0 0.05478255236927627 7.109487690317035E-4 48-49 0.0 0.0 0.0 0.09894037035691208 7.109487690317035E-4 50-51 0.0 0.0 0.0 0.12650938373380816 7.109487690317035E-4 52-53 0.0 0.0 0.0 0.15095812195773173 7.109487690317035E-4 54-55 0.0 0.0 0.0 0.19077125302350711 7.109487690317035E-4 56-57 0.0 0.0 0.0 0.22134205009187038 7.109487690317035E-4 58-59 0.0 0.0 0.0 0.26000976369642803 7.109487690317035E-4 60-61 0.0 0.0 0.0 0.327194422369924 7.109487690317035E-4 62-63 0.0 0.0 0.0 0.37395905251067607 7.109487690317035E-4 64-65 0.0 0.0 0.0 0.4275171931110644 7.109487690317035E-4 66-67 0.0 0.0 0.0 0.531236719082023 7.109487690317035E-4 68-69 0.0 0.0 0.0 0.6527694614326092 7.109487690317035E-4 70-71 0.0 0.0 0.0 0.7473256477138257 7.109487690317035E-4 72-73 0.0 0.0 0.0 0.8508476879156088 8.689373843720822E-4 74-75 0.0 0.0 0.0 0.9374254491221363 8.689373843720822E-4 76-77 0.0 0.0 0.0 1.0009763696428036 8.689373843720822E-4 78-79 0.0 0.0 0.0 1.0884230682337033 8.689373843720822E-4 80-81 0.0 0.0 0.0 1.2382357727302171 8.689373843720822E-4 82-83 0.0 0.0 0.0 1.4107988378357454 8.689373843720822E-4 84-85 0.0 0.0 0.0 1.613735214240462 8.689373843720822E-4 86-87 0.0 0.0 0.0 1.9737122742935145 8.689373843720822E-4 88-89 0.0 0.0 0.0 2.4209780443221263 8.689373843720822E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1305 0.0 28.753271 2 GTACCTG 1420 0.0 26.766552 1 TAAGGTG 495 0.0 23.983894 5 GTACATG 7320 0.0 21.159105 1 GAGTACT 5905 0.0 21.029875 12-13 AGTACTT 6050 0.0 20.839823 12-13 TACATGG 7405 0.0 20.461445 2 ACATGGG 7105 0.0 20.455536 3 GTACTTT 6360 0.0 19.52538 14-15 ACGATCG 75 7.703728E-4 19.007631 82-83 ATGGGAG 1875 0.0 18.741974 5 ACCTGGG 1890 0.0 18.596167 3 TGGACCG 155 9.641454E-4 18.382494 5 AACGCGA 65 0.007561849 18.27079 76-77 TAACGCA 130 0.0075744675 18.264656 4 CATGGGA 4995 0.0 18.158617 4 GTAAGGT 455 0.0 17.742811 4 TACTTTT 7275 0.0 17.265438 14-15 CATTCCG 195 2.2798973E-4 17.047014 9 CATGGGG 2370 0.0 17.0316 4 >>END_MODULE