FastQCFastQC Report
Thu 26 May 2016
SRR936535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092618
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.2065680777728355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.1720637953978426No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17360.15888444085673126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13530.12383101870919205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG41850.029.8561231
TACATGG42300.029.0905862
ACATGGG41650.028.519243
TATACCG908.9329184E-426.396145
CATGGGA21600.023.9764944
GGTATCA41350.023.8979661
GTATCAA56100.023.3732661
GAGTACT28600.022.92220512-13
GTATAGG4000.022.5666311
ATACGAC1100.00287269221.5968423
TCAACGC62850.020.864924
CATGGGG21650.020.8486844
CAACGCA63050.020.7987355
GTACTTT30500.020.79669614-15
ATCAACG63400.020.6839143
GCGTCGC1150.003714281820.6606867
GTACCCG1150.003719213220.6559561
TAGGGTG3252.1827873E-1120.467165
TATCAAC64200.020.4252362
AACGCAG64700.020.3426886
Produced by FastQC (version 0.11.5)