##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936535_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1092618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.582719669637513 31.0 31.0 34.0 30.0 34.0 2 31.683274483854376 33.0 31.0 34.0 30.0 34.0 3 31.70410152496115 33.0 31.0 34.0 30.0 34.0 4 35.32295825256403 37.0 35.0 37.0 33.0 37.0 5 35.324090395728426 37.0 35.0 37.0 33.0 37.0 6 35.304046794030484 37.0 35.0 37.0 33.0 37.0 7 35.23614383068922 37.0 35.0 37.0 32.0 37.0 8 35.20309293824557 37.0 35.0 37.0 32.0 37.0 9 36.7366462935811 39.0 37.0 39.0 32.0 39.0 10-11 36.62411107999319 39.0 37.0 39.0 32.0 39.0 12-13 36.53926395135353 39.0 37.0 39.0 32.0 39.0 14-15 37.69226024099914 40.0 37.0 41.0 32.0 41.0 16-17 37.6007520469185 40.0 37.0 41.0 32.0 41.0 18-19 37.54155340658858 40.0 37.0 41.0 32.0 41.0 20-21 37.38852782948844 40.0 37.0 41.0 32.0 41.0 22-23 37.50237594474922 40.0 37.0 41.0 32.0 41.0 24-25 37.53753965246774 40.0 37.0 41.0 32.0 41.0 26-27 37.45847405040005 40.0 37.0 41.0 32.0 41.0 28-29 37.32383413050124 40.0 37.0 41.0 31.5 41.0 30-31 37.14675119758232 39.5 36.5 41.0 31.0 41.0 32-33 36.96651391428661 39.0 36.0 41.0 30.5 41.0 34-35 36.77883121090811 39.0 36.0 41.0 30.0 41.0 36-37 36.591830813697015 39.0 36.0 41.0 30.0 41.0 38-39 36.40999004226546 39.0 35.0 40.0 30.0 41.0 40-41 36.20331305177107 39.0 35.0 40.0 30.0 41.0 42-43 35.99334259549083 39.0 35.0 40.0 29.0 41.0 44-45 35.769004812294874 38.0 35.0 40.0 28.5 41.0 46-47 35.65781590638265 39.0 35.0 40.0 27.5 41.0 48-49 35.54085462622801 39.0 35.0 40.0 27.0 41.0 50-51 34.614577098308835 38.0 34.0 39.5 25.5 40.5 52-53 34.75363301721187 38.0 34.0 39.5 26.0 40.5 54-55 35.194807791927275 38.0 35.0 40.0 26.5 41.0 56-57 35.087905837172734 38.0 34.0 40.0 26.0 41.0 58-59 34.86680569055241 38.0 34.0 40.0 26.0 41.0 60-61 34.58420463510578 38.0 34.0 40.0 26.0 41.0 62-63 34.23522310633726 37.5 34.0 40.0 25.0 41.0 64-65 33.86383438676646 37.0 33.0 40.0 23.5 41.0 66-67 33.39536095872482 36.0 33.0 39.0 22.0 41.0 68-69 33.00547446591581 36.0 32.0 39.0 22.0 40.5 70-71 32.60703786684825 35.5 32.0 39.0 21.0 40.0 72-73 32.212674054427076 35.0 31.5 38.0 20.0 40.0 74-75 31.761563510760396 35.0 31.0 37.0 18.5 39.0 76-77 31.274533276954983 35.0 31.0 37.0 17.5 39.0 78-79 30.76956676532878 34.5 30.5 36.0 12.5 39.0 80-81 30.298582395677172 34.0 30.0 36.0 8.0 37.5 82-83 29.869626438517397 34.0 29.5 35.0 7.0 37.0 84-85 29.491829715417467 34.0 29.0 35.0 4.0 36.5 86-87 29.12830101645772 34.0 29.0 35.0 2.0 36.0 88-89 28.706191001795688 34.0 29.0 35.0 2.0 36.0 90-91 28.296351515351205 34.0 28.5 35.0 2.0 35.5 92-93 27.840332577350914 34.0 27.0 35.0 2.0 35.0 94-95 27.31018343098869 33.0 26.0 35.0 2.0 35.0 96-97 26.71758565207602 33.0 25.0 35.0 2.0 35.0 98-99 25.946711934088583 33.0 24.5 35.0 2.0 35.0 100-101 23.07569571433017 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2969426361169205 1101 2 0.2317163647853171 1101 3 0.2558317802293466 1101 4 0.30692425524760125 1101 5 0.20792459340400882 1101 6 0.31412069992556724 1101 7 0.2743191457715284 1101 8 0.2221356653642701 1101 9 0.2211655602010154 1101 10-11 0.4373824325208844 1101 12-13 0.4229269261877491 1101 14-15 0.527021967378225 1101 16-17 0.420650206307279 1101 18-19 0.35625864644772065 1101 20-21 0.4271450468623428 1101 22-23 0.46756660841899844 1101 24-25 0.40606491268984257 1101 26-27 0.2212610560260444 1101 28-29 0.19577012335863486 1101 30-31 0.414216835495381 1101 32-33 0.19062511382069403 1101 34-35 0.10789456293588273 1101 36-37 0.09851697343516008 1101 38-39 0.009306799360835782 1101 40-41 -0.1728068859609735 1101 42-43 0.10159880023302748 1101 44-45 -0.0471624761104934 1101 46-47 0.17777185534837514 1101 48-49 0.3785380407441963 1101 50-51 0.023642550987446498 1101 52-53 0.2087562515774053 1101 54-55 0.10248361059743871 1101 56-57 0.029001732287753157 1101 58-59 0.10393023470923879 1101 60-61 0.09204084652183298 1101 62-63 0.05802914585005681 1101 64-65 0.16321127038530392 1101 66-67 0.14862808175099929 1101 68-69 -0.030258745396103848 1101 70-71 -0.07119401191992125 1101 72-73 -0.17554824792624402 1101 74-75 -0.18242654944904046 1101 76-77 -0.09115389762069981 1101 78-79 -0.10456879322738999 1101 80-81 -0.10128107467559389 1101 82-83 -0.2578069550332529 1101 84-85 -0.28917216289495684 1101 86-87 -0.17030580198305856 1101 88-89 -0.2915460204259084 1101 90-91 -0.3805809141062504 1101 92-93 -0.36055407916343185 1101 94-95 -0.41876919854563965 1101 96-97 -0.40414103789919054 1101 98-99 -0.6652058385172666 1101 100-101 -0.40724708432663803 1102 1 0.07941509854228457 1102 2 0.21783441453764496 1102 3 0.10437511973933056 1102 4 0.14270743355907456 1102 5 0.1939801953853859 1102 6 0.22051224820640414 1102 7 0.22035670223944948 1102 8 0.2847003839632549 1102 9 0.16196015320807788 1102 10-11 0.20418958774863682 1102 12-13 0.3581021659871908 1102 14-15 0.2812854470134383 1102 16-17 0.07418921610513962 1102 18-19 0.1353744465664093 1102 20-21 0.11002277639764202 1102 22-23 0.009878757954531636 1102 24-25 0.046352316255344306 1102 26-27 -0.26345835289255604 1102 28-29 -0.3836384621879958 1102 30-31 0.012371484940082667 1102 32-33 0.056349159770142876 1102 34-35 -0.08345959994608165 1102 36-37 -0.3499838637434536 1102 38-39 -0.2353007409993495 1102 40-41 -0.2581065671367142 1102 42-43 -0.24435238907530277 1102 44-45 -0.40467804642138816 1102 46-47 -0.11758433916772049 1102 48-49 0.014020561423862432 1102 50-51 0.08437811760445157 1102 52-53 0.09377628028067164 1102 54-55 0.14840297046615802 1102 56-57 0.1292421372823611 1102 58-59 -0.1300969501301097 1102 60-61 -0.03236476424007151 1102 62-63 -0.022828227982735427 1102 64-65 0.09129830970998398 1102 66-67 0.15855235720547256 1102 68-69 0.08396863564278334 1102 70-71 -0.2050519443764074 1102 72-73 -0.37795982945199924 1102 74-75 -0.3663522661223695 1102 76-77 -0.1889382128600765 1102 78-79 -0.25642114550245765 1102 80-81 0.17020947413812948 1102 82-83 0.012497275367984173 1102 84-85 -0.32748084260526156 1102 86-87 -0.12244564108732803 1102 88-89 -0.17203782879224505 1102 90-91 -0.12222691623780335 1102 92-93 0.04705028052362081 1102 94-95 0.030501638572410172 1102 96-97 -0.10426457094427732 1102 98-99 -0.41184028213412205 1102 100-101 -0.302375325555186 1103 1 0.12040818584905821 1103 2 -0.07649495176145393 1103 3 0.03288045394355521 1103 4 0.17589692588114758 1103 5 0.23272681748294843 1103 6 0.24868691617940186 1103 7 0.2790857784551761 1103 8 0.22629108784768448 1103 9 -0.07739024893841417 1103 10-11 0.18057308693537522 1103 12-13 -0.2216305394725211 1103 14-15 0.03562349019936306 1103 16-17 0.0724786527158372 1103 18-19 0.36683520526570845 1103 20-21 0.3724966016560245 1103 22-23 0.11728264782531284 1103 24-25 0.05950832571986808 1103 26-27 -0.04143991210671061 1103 28-29 0.016794261522001364 1103 30-31 -0.08091345313703613 1103 32-33 -0.13607561386236 1103 34-35 0.18245716437559167 1103 36-37 0.13302916919671048 1103 38-39 0.0024011326959154644 1103 40-41 -0.06591852683241939 1103 42-43 -0.14195111667869043 1103 44-45 0.07656038706435453 1103 46-47 0.17324365515868578 1103 48-49 0.12182007410940798 1103 50-51 0.034867032961585664 1103 52-53 0.24734156825085307 1103 54-55 0.23177292442422726 1103 56-57 0.3519115446926122 1103 58-59 0.2495890890471486 1103 60-61 0.15088304092378735 1103 62-63 0.08171465056646099 1103 64-65 0.2467590652074847 1103 66-67 0.31446816899066476 1103 68-69 -0.08609854619403023 1103 70-71 -0.14079955743208217 1103 72-73 -0.24601291695087468 1103 74-75 -0.20806711992803173 1103 76-77 -0.04521146595621772 1103 78-79 -0.1744111574771985 1103 80-81 -0.19188986020697385 1103 82-83 -0.4524013555409425 1103 84-85 -0.2722531383622311 1103 86-87 -0.26747632236702046 1103 88-89 -0.2717393807062827 1103 90-91 -0.1662296986048304 1103 92-93 -0.20351783944154533 1103 94-95 -0.03166577411441551 1103 96-97 -0.14410781065688383 1103 98-99 -0.07305127616596252 1103 100-101 0.14589754172366654 1104 1 0.23532634515475337 1104 2 0.18104480031797365 1104 3 0.1839821658097911 1104 4 0.33200581551348307 1104 5 0.3280667568392346 1104 6 0.33949937557767385 1104 7 0.3141116839069156 1104 8 0.3907252125938996 1104 9 0.24410286801757763 1104 10-11 0.5137902572847466 1104 12-13 0.44070070336962175 1104 14-15 0.17909650232987673 1104 16-17 0.26696978361669466 1104 18-19 0.21690441627787038 1104 20-21 0.0751660491538928 1104 22-23 0.1107998682685789 1104 24-25 0.0866614785381401 1104 26-27 0.06318709187071647 1104 28-29 0.222560724803877 1104 30-31 0.18855811812995427 1104 32-33 -0.0485199220089072 1104 34-35 0.16120752503399416 1104 36-37 -0.10847013426791818 1104 38-39 -0.03256680189525696 1104 40-41 0.08078381625241349 1104 42-43 -0.048119526011383584 1104 44-45 -0.15074003829129623 1104 46-47 -0.18893925308450576 1104 48-49 -0.0406233489858181 1104 50-51 -0.016550465210784182 1104 52-53 0.20883785369052532 1104 54-55 0.0063831035763186605 1104 56-57 0.02729729430451755 1104 58-59 -0.053416633694595816 1104 60-61 0.014689575788622733 1104 62-63 0.08313183897087129 1104 64-65 0.18894397366662474 1104 66-67 0.09228621660288638 1104 68-69 -0.0015391955384700395 1104 70-71 9.72386087482846E-4 1104 72-73 -0.23503828075121902 1104 74-75 -0.15780970275555717 1104 76-77 -0.19181512582037996 1104 78-79 -0.06422094225042585 1104 80-81 0.2155899738990108 1104 82-83 -0.06810610419522334 1104 84-85 -0.10372675763793993 1104 86-87 -0.10335838220003168 1104 88-89 -0.28301692571556814 1104 90-91 -0.479138481676987 1104 92-93 -0.7096824430536159 1104 94-95 -0.6960795426805788 1104 96-97 -0.40982012649983446 1104 98-99 -0.4141343400011692 1104 100-101 -0.34841091458755 1105 1 0.28099354949656785 1105 2 0.22033026973725 1105 3 0.31614831103982155 1105 4 0.28230153684619097 1105 5 0.1811704776234464 1105 6 0.33071253671062095 1105 7 0.2953580076883924 1105 8 0.5330873386119634 1105 9 0.5038613111412928 1105 10-11 0.5070472154868142 1105 12-13 0.42635307653782917 1105 14-15 0.43805925840216986 1105 16-17 0.45581600130656597 1105 18-19 0.3584870937557767 1105 20-21 0.3375258115664366 1105 22-23 0.075102658766518 1105 24-25 0.39471911803597237 1105 26-27 0.037212239911809775 1105 28-29 -0.0559919908164872 1105 30-31 3.0505717057849324E-4 1105 32-33 -0.15242068305380485 1105 34-35 -0.015534235725823464 1105 36-37 -0.0449049330366762 1105 38-39 -0.049417865878595535 1105 40-41 -0.04905074940349863 1105 42-43 0.026253306598441384 1105 44-45 0.042843313986956844 1105 46-47 0.14461059373220309 1105 48-49 0.19480484251313612 1105 50-51 0.29988489288447084 1105 52-53 0.3368027352035199 1105 54-55 0.2852641979797994 1105 56-57 0.08670408344564606 1105 58-59 0.26630792506897194 1105 60-61 0.3442176846515963 1105 62-63 0.3493960612003093 1105 64-65 0.27556650766826607 1105 66-67 0.4184231830324663 1105 68-69 0.4176295550704694 1105 70-71 0.1951876870953484 1105 72-73 0.2745325173535704 1105 74-75 0.30192826180118715 1105 76-77 0.03717900666422125 1105 78-79 0.12273303780868261 1105 80-81 0.28743185410209904 1105 82-83 -0.033258710115422474 1105 84-85 -0.012089681147337217 1105 86-87 -0.22088505846545914 1105 88-89 -0.12765542036948574 1105 90-91 -0.1620080250861342 1105 92-93 -0.33551601996461855 1105 94-95 -0.3261527242379607 1105 96-97 -0.3209756228750358 1105 98-99 -0.057305234093597335 1105 100-101 0.3784677007365538 1106 1 0.32505039638765965 1106 2 0.13678943951341438 1106 3 0.2424296191056179 1106 4 0.25501219386623575 1106 5 0.24186471040812307 1106 6 0.26871408597573065 1106 7 0.47088417558619966 1106 8 0.32637031239089964 1106 9 0.47775872548869813 1106 10-11 0.524132926300787 1106 12-13 0.5337417041218373 1106 14-15 0.5552915418468061 1106 16-17 0.5571227507357221 1106 18-19 0.45303175663872963 1106 20-21 0.21101388576914815 1106 22-23 0.5191172299593063 1106 24-25 0.36897752128032124 1106 26-27 0.38410814043480457 1106 28-29 0.4145079256447062 1106 30-31 0.47483280977420605 1106 32-33 0.31338384626831584 1106 34-35 0.3730105689199519 1106 36-37 0.2106739402526756 1106 38-39 0.20451883702197904 1106 40-41 0.5028620360840392 1106 42-43 0.273583102611866 1106 44-45 0.03022768868634529 1106 46-47 0.33325463235244257 1106 48-49 0.5073083232966766 1106 50-51 0.044694601612512486 1106 52-53 0.18364851244350433 1106 54-55 0.33834905821684913 1106 56-57 0.26272085196214334 1106 58-59 0.32913872607953465 1106 60-61 0.46952589221261576 1106 62-63 0.6122617966202597 1106 64-65 0.45081193455155955 1106 66-67 0.5976944208750368 1106 68-69 0.6580476541323108 1106 70-71 0.2741214787951449 1106 72-73 -0.04796397654809681 1106 74-75 0.2876932494222544 1106 76-77 0.22051833116438146 1106 78-79 0.08573268846459214 1106 80-81 0.5479109111768246 1106 82-83 0.4922370800350997 1106 84-85 0.5570991823705072 1106 86-87 0.4652153501152405 1106 88-89 0.44165900450381557 1106 90-91 0.3033821011334048 1106 92-93 0.428803290663037 1106 94-95 0.30224034356010776 1106 96-97 0.5398418929340494 1106 98-99 0.8286668285048115 1106 100-101 0.7856563414842519 1107 1 0.1884887900023422 1107 2 0.1235697270698708 1107 3 0.257048548239208 1107 4 0.2844734468764969 1107 5 0.24740272893674842 1107 6 0.26224935063565624 1107 7 0.36841912029457546 1107 8 0.36584376355401105 1107 9 0.30256696881185263 1107 10-11 0.3936829658662262 1107 12-13 0.2790891229412722 1107 14-15 -0.06613549920125905 1107 16-17 0.028851603223778 1107 18-19 0.10538354336816269 1107 20-21 0.1317073236199846 1107 22-23 0.15609534470193154 1107 24-25 0.17446783274744604 1107 26-27 -0.022576561596025613 1107 28-29 0.06456777372485334 1107 30-31 0.02854639028915784 1107 32-33 -0.05231849023500956 1107 34-35 -0.0686922589375456 1107 36-37 0.15726802743375856 1107 38-39 -0.05452850723732183 1107 40-41 -0.026316973951587386 1107 42-43 0.23386454570762538 1107 44-45 0.3570182032655751 1107 46-47 0.2599331638890945 1107 48-49 0.1765526948632754 1107 50-51 0.181292202547219 1107 52-53 0.33789144137140426 1107 54-55 0.3362388063407096 1107 56-57 0.20327242558099812 1107 58-59 0.07665295963845153 1107 60-61 0.07719530227338112 1107 62-63 0.07448637404139191 1107 64-65 0.0713336841152028 1107 66-67 0.2857390883968165 1107 68-69 0.32397481373384807 1107 70-71 0.15898977016815508 1107 72-73 -0.011606624947056332 1107 74-75 0.07235757168202639 1107 76-77 0.04334258920530587 1107 78-79 -0.028510583268545275 1107 80-81 0.052773032055657865 1107 82-83 -0.056472686771641634 1107 84-85 -0.08925351279279425 1107 86-87 0.0447990131605529 1107 88-89 0.1680990007329548 1107 90-91 0.09774581177150665 1107 92-93 0.030212220167996406 1107 94-95 0.18976794652308726 1107 96-97 0.2642553388979465 1107 98-99 0.2243297709735259 1107 100-101 0.2976650944445183 1108 1 0.14766191290660657 1108 2 0.13405549680200224 1108 3 0.19621554835213217 1108 4 0.29395006571184723 1108 5 0.23769112024186256 1108 6 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-2.7840225326117043 2316 72-73 -2.7128617902363104 2316 74-75 -2.527418442592957 2316 76-77 -2.673288703056155 2316 78-79 -3.1447544788357895 2316 80-81 -3.0907464082148124 2316 82-83 -2.981056646200642 2316 84-85 -2.6751156465268267 2316 86-87 -2.708793680303014 2316 88-89 -2.9713638091843997 2316 90-91 -3.261736720159373 2316 92-93 -4.035866386143045 2316 94-95 -4.023432184957002 2316 96-97 -4.611764570944278 2316 98-99 -6.1439971448792186 2316 100-101 -6.712240521633618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2517.0 3 984.0 4 1000.0 5 1044.0 6 1160.0 7 1343.0 8 1629.0 9 1965.0 10 2542.0 11 3179.0 12 3782.0 13 4580.0 14 5182.0 15 6198.0 16 7211.0 17 9082.0 18 7683.0 19 6210.0 20 6291.0 21 6924.0 22 7637.0 23 8797.0 24 10186.0 25 11465.0 26 13388.0 27 15732.0 28 18874.0 29 22923.0 30 27970.0 31 34323.0 32 44149.0 33 57635.0 34 77647.0 35 108339.0 36 156572.0 37 210195.0 38 163900.0 39 22379.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.370830617819145 18.178323240163735 14.207478709148294 23.243367432868826 2 15.437416189547779 19.36979116609229 40.8467773307887 24.34601531357123 3 19.795782931260955 23.618147949819903 29.339338102364838 27.246731016554303 4 12.6142661244995 15.78186132262181 37.40175356048942 34.20211899238927 5 13.802328189587692 36.90093521260866 34.569815932946675 14.726920664856971 6 32.60814823700266 35.14045100719813 17.836000545743065 14.41540021005614 7 29.343855515862444 31.730293103558743 20.51981192605549 18.40603945452332 8 26.49228521364658 34.28479618572614 19.898692505296076 19.324226095331213 9 26.01948840867246 16.7762733247943 20.466857528434712 36.73738073809853 10-11 25.035296961439784 25.945601476704006 27.757695286962925 21.261406274893286 12-13 25.96529342750406 23.24531950160743 28.111646234475586 22.677740836412923 14-15 22.710223647144883 24.94870919279576 26.302502698432022 26.038564461627335 16-17 22.302796489602656 28.268018434529772 26.93312630802217 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22.227840918191532 26.34048229767546 28.554273933547353 22.877402850585653 44-45 22.339689437469225 26.45035044911303 28.437952476574186 22.77200763684356 46-47 22.50714688747159 26.68590808143997 28.114507620982266 22.69243741010618 48-49 22.594669001139017 26.687805661583575 27.993212579608727 22.72431275766868 50-51 22.314363877826906 26.848947583748938 28.131552717830534 22.705135820593622 52-53 22.614466961868448 26.887814780712915 27.7748387769933 22.72287948042534 54-55 22.26880969019334 26.62611085712246 28.025473917653677 23.079605535030527 56-57 22.413181629517904 26.90585812065862 27.840408049887817 22.840552199935658 58-59 22.721637538767663 26.616869545240498 27.846680270720363 22.81481264527148 60-61 22.526143143291105 26.589422111944046 28.37952554044722 22.504909204317627 62-63 22.290346435880256 26.791852917595634 28.262914923138315 22.654885723385796 64-65 22.249155691417798 26.83166912255974 28.017499382213234 22.901675803809226 66-67 22.206615379193654 27.024415784605427 27.64731646970465 23.121652366496274 68-69 22.140338299930463 26.73919024644375 28.13326173449558 22.98720971913021 70-71 22.264734725944674 26.772392928466974 27.883649529300055 23.079222816288297 72-73 22.217935269144373 26.585959053953363 28.133335530206082 23.06277014669618 74-75 22.07672895574886 27.10112931480789 27.85728639443669 22.96485533500656 76-77 22.279201001174567 26.830169264050635 27.736453795006394 23.1541759397684 78-79 22.234813313041073 26.78582875017628 27.837894513214696 23.141463423567956 80-81 22.33800361892558 26.95943844826773 27.717892339940857 22.984665592865834 82-83 22.235082063131543 26.884998074839082 27.895879239989508 22.984040622039863 84-85 22.228990988945803 26.77401696758437 27.80368827098338 23.19330377248644 86-87 22.255440876948075 26.815753941918206 27.865436986520812 23.063368194612902 88-89 22.216943371510407 26.75983685605078 27.520494832752735 23.50272493968608 90-91 21.98945353682816 26.62659212087003 28.02933855941301 23.354615782888803 92-93 22.201431650563382 26.632234062371406 28.012162484420422 23.154171802644786 94-95 22.03580191371311 26.602049367335983 27.97799692169264 23.384151797258266 96-97 21.86277659844218 26.457608453868144 28.164181823341323 23.515433124348352 98-99 21.847970363465123 26.660015448286472 27.81071590949154 23.681298278756866 100-101 21.897828603667964 26.43318752335524 27.900446222495766 23.768537650481026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 49.0 1 38.5 2 39.5 3 60.0 4 68.0 5 78.5 6 125.5 7 225.0 8 369.0 9 492.0 10 498.0 11 473.5 12 530.5 13 625.5 14 672.0 15 742.0 16 802.0 17 872.5 18 978.0 19 1042.5 20 1204.5 21 1417.0 22 1685.5 23 2067.0 24 2517.0 25 3122.5 26 4123.0 27 5515.0 28 6951.5 29 8721.0 30 10983.0 31 13580.0 32 16341.5 33 18923.0 34 21458.5 35 23780.5 36 26570.5 37 29366.5 38 31060.5 39 32140.5 40 33252.0 41 35549.5 42 37724.0 43 39435.5 44 41296.5 45 42949.0 46 43471.5 47 44571.5 48 48341.0 49 47794.0 50 43526.5 51 40654.0 52 38504.0 53 37433.0 54 35998.5 55 33290.5 56 30036.0 57 26658.5 58 23769.0 59 20848.0 60 17303.0 61 13843.0 62 11167.0 63 8787.5 64 6549.5 65 4922.5 66 3670.5 67 2899.0 68 2173.0 69 1477.0 70 1092.0 71 827.0 72 639.0 73 511.0 74 409.0 75 317.0 76 217.5 77 147.0 78 81.0 79 51.5 80 37.5 81 24.5 82 19.0 83 13.5 84 12.0 85 7.5 86 6.5 87 6.5 88 4.0 89 3.5 90 3.5 91 2.0 92 1.5 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011898028405169968 2 0.011257365337199277 3 0.014003064199930807 4 0.019402938629969488 5 0.03230772328480768 6 0.04841582327949933 7 0.006315107384282521 8 0.027456988627315312 9 0.034687328965841674 10-11 0.04173462271351927 12-13 0.031712821864549184 14-15 0.029012884649529846 16-17 0.029287454535803 18-19 0.02228592243583759 20-21 0.05235132498274785 22-23 0.010616702269228586 24-25 0.0023796056810339934 26-27 0.0011898028405169967 28-29 4.576164771219218E-5 30-31 0.0020592741470486483 32-33 0.0015101343745023421 34-35 0.0 36-37 3.203315339853453E-4 38-39 4.576164771219218E-5 40-41 0.0012813261359413811 42-43 0.003249076987565645 44-45 0.0018304659084876875 46-47 8.694713065316515E-4 48-49 7.779480111072671E-4 50-51 0.0033863619307022215 52-53 0.0029287454535802996 54-55 0.004530403123507026 56-57 5.949014202584984E-4 58-59 0.004713449714355795 60-61 0.00251689062417057 62-63 5.491397725463063E-4 64-65 7.321863633950749E-4 66-67 0.013591209370521079 68-69 0.038577069021378016 70-71 0.047683636916104256 72-73 0.014277634086203961 74-75 0.029150169592666423 76-77 0.027548511922739695 78-79 0.0527174181644454 80-81 0.0784812258264096 82-83 0.04589893265532876 84-85 0.016657239767237954 86-87 0.017663996016906183 88-89 0.019082607095984142 90-91 0.03692964970373909 92-93 0.02219439914041321 94-95 0.012309883234579697 96-97 0.01784704260775495 98-99 0.05376993606182581 100-101 0.07193731020356611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1092618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.09842273379402 #Duplication Level Percentage of deduplicated Percentage of total 1 84.71895188387809 57.692269989522856 2 9.434739413243323 12.849817458924631 3 2.524475540557419 5.15738407626007 4 1.0527242560498218 2.8675544564239837 5 0.569017688211161 1.937460353740492 6 0.35895013327231823 1.466636274955802 7 0.2397361364964342 1.1427956937390504 8 0.17899297676455625 0.9751311518474338 9 0.12842476293319446 0.7870971416140762 >10 0.7231054385455258 9.089939194791926 >50 0.04700061474737583 2.188295509178833 >100 0.022666860539434924 2.8059101766110937 >500 5.396865606162784E-4 0.27692483973866094 >1k 6.746082007703481E-4 0.7627836826510827 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2257 0.2065680777728355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.1720637953978426 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1736 0.15888444085673126 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1353 0.12383101870919205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3728494313657654E-4 0.0 18-19 1.8304659084876875E-4 0.0 0.0 1.8304659084876872E-4 0.0 20-21 2.745698862731531E-4 0.0 0.0 1.8304659084876872E-4 9.152329542438436E-5 22-23 2.745698862731531E-4 0.0 0.0 3.203315339853453E-4 9.152329542438436E-5 24-25 3.203315339853453E-4 0.0 0.0 5.03378124834114E-4 9.152329542438436E-5 26-27 3.6609318169753745E-4 0.0 0.0 9.609946019560359E-4 9.152329542438436E-5 28-29 6.406630679706906E-4 0.0 0.0 0.0020592741470486483 9.152329542438436E-5 30-31 6.864247156828827E-4 0.0 0.0 0.004713449714355795 9.152329542438436E-5 32-33 7.321863633950749E-4 0.0 0.0 0.007184578690814174 9.152329542438436E-5 34-35 7.321863633950749E-4 0.0 0.0 0.00947266107642378 9.152329542438436E-5 36-37 7.321863633950749E-4 0.0 0.0 0.014323395733916153 9.152329542438436E-5 38-39 7.321863633950749E-4 0.0 0.0 0.020226648288788944 9.152329542438436E-5 40-41 8.237096588194593E-4 0.0 0.0 0.03523646873838798 9.152329542438436E-5 42-43 8.694713065316515E-4 0.0 0.0 0.04731754373440672 1.3728494313657654E-4 44-45 0.0010982795450926125 0.0 0.0 0.0620527942977326 1.8304659084876872E-4 46-47 0.0012813261359413811 0.0 0.0 0.09239276673091602 1.8304659084876872E-4 48-49 0.0012813261359413811 0.0 0.0 0.11051437922494413 1.8304659084876872E-4 50-51 0.0012813261359413811 0.0 0.0 0.13188506870653788 1.8304659084876872E-4 52-53 0.0013270877836535733 0.0 0.0 0.15673364341425822 1.8304659084876872E-4 54-55 0.0013728494313657656 0.0 0.0 0.1890413666990659 1.8304659084876872E-4 56-57 0.0013728494313657656 0.0 0.0 0.2800612839986162 1.8304659084876872E-4 58-59 0.0013728494313657656 0.0 0.0 0.3607848305629232 1.8304659084876872E-4 60-61 0.0013728494313657656 0.0 0.0 0.4172089421920562 1.8304659084876872E-4 62-63 0.0013728494313657656 0.0 0.0 0.48557684387407124 1.8304659084876872E-4 64-65 0.0013728494313657656 0.0 0.0 0.5622276037919931 1.8304659084876872E-4 66-67 0.0015558960222145342 0.0 0.0 0.6857840526149122 1.8304659084876872E-4 68-69 0.0015558960222145342 0.0 0.0 0.836980536655995 1.8304659084876872E-4 70-71 0.0015558960222145342 0.0 0.0 0.9208158752647311 2.288082385609609E-4 72-73 0.0016016576699267263 0.0 0.0 1.0126137405753886 2.745698862731531E-4 74-75 0.0016474193176389186 0.0 0.0 1.1751133516013832 2.745698862731531E-4 76-77 0.0016474193176389186 0.0 0.0 1.3735816177291607 2.745698862731531E-4 78-79 0.0016474193176389186 0.0 0.0 1.5600145705086317 2.745698862731531E-4 80-81 0.001738942613063303 0.0 0.0 1.695835140918418 2.745698862731531E-4 82-83 0.001738942613063303 0.0 0.0 1.9045997777814387 2.745698862731531E-4 84-85 0.001738942613063303 0.0 0.0 2.2451579600555727 2.745698862731531E-4 86-87 0.001738942613063303 0.0 0.0 2.5857619039774193 2.745698862731531E-4 88-89 0.0018304659084876873 0.0 0.0 2.9963354072512076 2.745698862731531E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4185 0.0 29.856123 1 TACATGG 4230 0.0 29.090586 2 ACATGGG 4165 0.0 28.51924 3 TATACCG 90 8.9329184E-4 26.39614 5 CATGGGA 2160 0.0 23.976494 4 GGTATCA 4135 0.0 23.897966 1 GTATCAA 5610 0.0 23.373266 1 GAGTACT 2860 0.0 22.922205 12-13 GTATAGG 400 0.0 22.566631 1 ATACGAC 110 0.002872692 21.596842 3 TCAACGC 6285 0.0 20.86492 4 CATGGGG 2165 0.0 20.848684 4 CAACGCA 6305 0.0 20.798735 5 GTACTTT 3050 0.0 20.796696 14-15 ATCAACG 6340 0.0 20.683914 3 GCGTCGC 115 0.0037142818 20.660686 7 GTACCCG 115 0.0037192132 20.655956 1 TAGGGTG 325 2.1827873E-11 20.46716 5 TATCAAC 6420 0.0 20.425236 2 AACGCAG 6470 0.0 20.342688 6 >>END_MODULE