FastQCFastQC Report
Thu 26 May 2016
SRR936535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092618
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.26651583627580727No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27570.2523297254850277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.24271977946546736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.13344096472875241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG908.9396385E-426.3927421
GTACATG41900.025.6242661
TACCTGG9500.025.5002212
TACATGG42450.024.5055122
GTGTAGG5650.024.384091
ACATGGG42100.023.5788063
GTAAGGT4250.023.4686434
GTATCAA71900.023.1257691
GTACCTG10800.022.433831
GAGTACT39200.022.23501612-13
GGTATCA55200.021.4297561
GTACTTT41550.020.69211814-15
TCAACGC82000.020.1568684
ATCAACG82250.020.0955983
CAACGCA82700.020.0436845
ATACGTC1908.256544E-619.9983445
TATCAAC83250.019.9130862
AACGCAG84900.019.5242966
TTGCGTA1950.019.48645860-61
CATGGGA20000.018.7609464
Produced by FastQC (version 0.11.5)