##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936535_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1092618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.159621203384894 31.0 31.0 34.0 31.0 34.0 2 32.29770606012348 34.0 31.0 34.0 31.0 34.0 3 32.415617352084624 34.0 31.0 34.0 31.0 34.0 4 36.08100909924603 37.0 35.0 37.0 35.0 37.0 5 35.968698117731904 37.0 35.0 37.0 35.0 37.0 6 36.028323714234986 37.0 35.0 37.0 35.0 37.0 7 35.96845924193085 37.0 35.0 37.0 35.0 37.0 8 35.93596481112338 37.0 35.0 37.0 35.0 37.0 9 37.603509186193165 39.0 37.0 39.0 35.0 39.0 10-11 37.505262589486904 39.0 37.0 39.0 35.0 39.0 12-13 37.4008221537628 39.0 37.0 39.0 34.0 39.0 14-15 38.57831190772987 40.0 38.0 41.0 34.0 41.0 16-17 38.554050912578774 40.0 38.0 41.0 34.0 41.0 18-19 38.67008506175077 40.0 38.0 41.0 34.0 41.0 20-21 38.668874666168776 40.0 38.0 41.0 34.0 41.0 22-23 38.575467363708086 40.0 38.0 41.0 34.0 41.0 24-25 38.47137746220545 40.0 38.0 41.0 34.0 41.0 26-27 38.29354403826406 40.0 38.0 41.0 34.0 41.0 28-29 38.33368432517129 40.0 38.0 41.0 34.0 41.0 30-31 38.244906728609635 40.0 38.0 41.0 34.0 41.0 32-33 38.09033623828273 40.0 37.5 41.0 33.5 41.0 34-35 37.9276792071886 40.0 37.0 41.0 33.0 41.0 36-37 37.76970908405316 40.0 37.0 41.0 33.0 41.0 38-39 37.58868744611566 40.0 37.0 41.0 33.0 41.0 40-41 37.3849442348561 40.0 37.0 41.0 32.5 41.0 42-43 37.1557406156589 39.5 36.0 41.0 31.5 41.0 44-45 36.892241844816766 39.0 36.0 41.0 31.0 41.0 46-47 36.550518570991876 39.0 36.0 40.5 30.5 41.0 48-49 36.17853861093264 39.0 35.0 40.0 30.0 41.0 50-51 35.9125435422078 39.0 35.0 40.0 29.0 41.0 52-53 35.6717846493468 38.5 35.0 40.0 28.5 41.0 54-55 35.35642374553595 38.0 34.5 40.0 28.0 41.0 56-57 35.02878133071211 38.0 34.0 40.0 27.0 41.0 58-59 34.665977038635646 38.0 34.0 40.0 26.0 41.0 60-61 34.293854302235545 37.0 33.5 40.0 26.0 41.0 62-63 33.98515034531739 37.0 33.0 39.0 26.0 40.5 64-65 33.66953317627936 36.0 33.0 39.0 25.5 40.0 66-67 33.23094164657731 36.0 32.0 39.0 24.0 40.0 68-69 32.7689563049483 35.0 32.0 38.5 22.5 40.0 70-71 32.27296548290437 35.0 31.0 38.0 22.0 40.0 72-73 31.70752907237479 35.0 31.0 37.0 20.5 39.0 74-75 31.180544801568345 34.0 30.0 36.5 19.0 39.0 76-77 29.812125555317593 33.0 28.5 35.0 18.5 37.0 78-79 30.580310776501943 34.0 30.0 36.0 18.5 37.5 80-81 30.415012840718347 34.0 30.0 35.0 18.0 37.0 82-83 30.014474866787843 34.0 30.0 35.0 14.5 37.0 84-85 29.580377130891126 34.0 29.0 35.0 9.0 36.0 86-87 29.15810557761267 34.0 29.0 35.0 7.0 36.0 88-89 28.67888868753764 33.0 29.0 35.0 2.0 35.0 90-91 28.142880677418823 33.0 27.5 35.0 2.0 35.0 92-93 27.56761329211124 33.0 26.5 34.0 2.0 35.0 94-95 26.832050176731485 32.0 25.5 34.0 2.0 35.0 96-97 26.030305193580922 32.0 24.5 34.0 2.0 35.0 98-99 24.863301263570616 31.5 20.5 34.0 2.0 35.0 100-101 20.997743035534835 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11360235238535665 1101 2 0.2794026974519852 1101 3 0.15533922978065817 1101 4 0.07826054667294358 1101 5 0.09692815677479416 1101 6 0.10453149353935487 1101 7 0.13682257307199563 1101 8 0.12852935313665625 1101 9 0.1410857260324505 1101 10-11 0.11691020807916885 1101 12-13 0.18284478766293688 1101 14-15 0.2113313500390106 1101 16-17 0.14824806221049158 1101 18-19 0.08063013421428877 1101 20-21 0.15266063143425157 1101 22-23 0.026383520120461412 1101 24-25 0.041235863869026446 1101 26-27 -0.035404626054358346 1101 28-29 0.0030633039433283216 1101 30-31 0.17013224620475143 1101 32-33 0.1292802313968835 1101 34-35 0.06273128377694093 1101 36-37 0.1427835268551121 1101 38-39 0.0777207149902921 1101 40-41 0.201003242065255 1101 42-43 0.19763571832331905 1101 44-45 0.37211933604203296 1101 46-47 0.31483293009463154 1101 48-49 0.4881652351009862 1101 50-51 0.4905793961548568 1101 52-53 0.3465595768230756 1101 54-55 0.4436072987426911 1101 56-57 0.5334817800635179 1101 58-59 0.37925062994461456 1101 60-61 0.3212819324961558 1101 62-63 0.2765276775212371 1101 64-65 0.24077204860241608 1101 66-67 0.1251198079099538 1101 68-69 0.056835025917102655 1101 70-71 -0.04913382482619255 1101 72-73 0.09136016851093487 1101 74-75 0.01534077322553884 1101 76-77 0.028570983289476715 1101 78-79 0.19103099411891833 1101 80-81 0.254051639038547 1101 82-83 0.1129731880833198 1101 84-85 -0.1397972785477286 1101 86-87 0.09398895902558024 1101 88-89 -0.06234906895645409 1101 90-91 -0.1292439177261997 1101 92-93 -0.04122955433560094 1101 94-95 -0.003735921649425933 1101 96-97 -0.12882116654425246 1101 98-99 -0.8303090161601183 1101 100-101 -0.46772655682939757 1102 1 0.11618920398826305 1102 2 0.230138218652435 1102 3 0.142088948197987 1102 4 0.07600182972105785 1102 5 -0.05351912065378173 1102 6 -0.019378130532636817 1102 7 0.11722721948893167 1102 8 -0.049373370645874104 1102 9 -0.03298384002745536 1102 10-11 0.033986759206108275 1102 12-13 0.06441031921836782 1102 14-15 0.10336937278362512 1102 16-17 0.06380524014652167 1102 18-19 0.027308253423676376 1102 20-21 0.005456096439282021 1102 22-23 -0.09754710911084885 1102 24-25 -0.011631283607627552 1102 26-27 -0.028511338319248125 1102 28-29 0.04960430298655183 1102 30-31 0.17283263920939618 1102 32-33 0.20504319176942687 1102 34-35 0.2529490864631896 1102 36-37 0.11207209157068121 1102 38-39 0.04976228022664486 1102 40-41 0.10110119044782095 1102 42-43 0.11801518882678153 1102 44-45 0.2104522757651779 1102 46-47 -0.011486383146419143 1102 48-49 0.286680529884741 1102 50-51 0.2164505236361265 1102 52-53 0.15917501058149952 1102 54-55 0.05340845747054601 1102 56-57 0.1459979029425611 1102 58-59 0.2920375869139349 1102 60-61 0.12586298743202207 1102 62-63 -0.24522439498781523 1102 64-65 -0.2665481548032247 1102 66-67 -0.2842671517843627 1102 68-69 -0.4711279505026127 1102 70-71 -0.5285941852520182 1102 72-73 -0.5117649450304675 1102 74-75 -0.29093185936054056 1102 76-77 -0.12842403754158482 1102 78-79 -0.10686852779608813 1102 80-81 0.03620372547534245 1102 82-83 0.06433015922260665 1102 84-85 -0.27700827783317195 1102 86-87 -0.14299162690860712 1102 88-89 -0.5732172519306964 1102 90-91 -0.29454997992872123 1102 92-93 -0.1655530368169238 1102 94-95 0.004064183414218547 1102 96-97 -0.024646362743794725 1102 98-99 -0.003600255911113237 1102 100-101 -0.305194311071304 1103 1 0.15854465858028988 1103 2 0.16661404183604844 1103 3 0.16111486359353222 1103 4 0.09094144371165669 1103 5 0.032648208786156374 1103 6 0.07400366209297005 1103 7 0.10261699100825439 1103 8 0.09148355997744773 1103 9 -0.09439762827299347 1103 10-11 0.012812033600646089 1103 12-13 0.0472664637989908 1103 14-15 0.22291590138853934 1103 16-17 0.09354979227828153 1103 18-19 0.16623909927606206 1103 20-21 0.15416424887073532 1103 22-23 0.048662636217713384 1103 24-25 -8.900341359279196E-4 1103 26-27 0.03847939085942187 1103 28-29 0.013512376013281369 1103 30-31 0.23942734534789167 1103 32-33 0.24394770279003097 1103 34-35 0.05173696379569748 1103 36-37 -0.06474028916214536 1103 38-39 -0.08058313030856823 1103 40-41 0.049972394710053436 1103 42-43 0.18639530050567288 1103 44-45 0.07417312334654014 1103 46-47 -0.0045322665879723445 1103 48-49 -0.040854137640003785 1103 50-51 0.15371018827924843 1103 52-53 0.15613209681246332 1103 54-55 0.0396881301822134 1103 56-57 -0.024480110274474498 1103 58-59 -0.0589652979107953 1103 60-61 -0.3158940101089911 1103 62-63 0.03487795865775212 1103 64-65 0.027979923410420326 1103 66-67 -0.0057640988649581 1103 68-69 -0.19514051437859337 1103 70-71 -0.42313879277497435 1103 72-73 -0.1798091875478498 1103 74-75 -0.1315893176410441 1103 76-77 -0.04048911259185317 1103 78-79 -0.26474764531507233 1103 80-81 -0.14048958819891766 1103 82-83 -0.23052190145796558 1103 84-85 -0.1656127398661873 1103 86-87 -0.21414530065766613 1103 88-89 -0.2621508593383872 1103 90-91 -0.03176638020535805 1103 92-93 -0.1466990182336545 1103 94-95 -0.22695452632310165 1103 96-97 -0.11348019395753184 1103 98-99 -0.25243660054040973 1103 100-101 -0.2933172218946787 1104 1 0.08146200009857552 1104 2 0.12181185965354757 1104 3 0.09088737000573133 1104 4 0.033239196212370814 1104 5 -0.0014258239855209354 1104 6 -0.055749284685354894 1104 7 -0.10790927819957119 1104 8 -0.099309605302345 1104 9 -0.0520735897484812 1104 10-11 0.09972982472499581 1104 12-13 -0.01455658258300474 1104 14-15 -0.004702921174605024 1104 16-17 0.03470981132458206 1104 18-19 0.11534179000904032 1104 20-21 0.04941931180673009 1104 22-23 -0.030321997521497224 1104 24-25 -0.22633118795960883 1104 26-27 -0.017618922409795346 1104 28-29 0.016610719374249072 1104 30-31 0.10307434971473839 1104 32-33 0.01979739629676658 1104 34-35 -0.05907765512776564 1104 36-37 -0.006381997381978977 1104 38-39 0.10374362886365418 1104 40-41 0.09053009208977869 1104 42-43 0.22124530201026715 1104 44-45 0.11076910981587673 1104 46-47 0.049984902522318464 1104 48-49 0.1443667446145369 1104 50-51 0.021176631558958547 1104 52-53 0.2350607510565581 1104 54-55 -0.06936722800689665 1104 56-57 0.14094151046687386 1104 58-59 0.23029978203589252 1104 60-61 -0.07596677902740367 1104 62-63 -0.2573522644282704 1104 64-65 -0.12175349913608358 1104 66-67 -0.08435532729846784 1104 68-69 -0.1586179871676876 1104 70-71 -0.3040305793218394 1104 72-73 0.05972198307410892 1104 74-75 0.1706295944892915 1104 76-77 0.004434542882453485 1104 78-79 0.12828859399571968 1104 80-81 0.04965173679369528 1104 82-83 0.06672634127266264 1104 84-85 0.15167417075602074 1104 86-87 0.2710048659974973 1104 88-89 0.17586612125597512 1104 90-91 0.27256802581015904 1104 92-93 0.43597583692769604 1104 94-95 0.541299656871896 1104 96-97 0.6048197021374442 1104 98-99 0.17946356921243378 1104 100-101 0.1370980530288044 1105 1 -0.022213677137557397 1105 2 0.1226761093080313 1105 3 0.10448712318036257 1105 4 0.00580784000799639 1105 5 0.0941116526941741 1105 6 0.07988739125530486 1105 7 0.00820509865658181 1105 8 -0.08782536928821116 1105 9 -0.022873137957013512 1105 10-11 0.14286201703227164 1105 12-13 0.09467266550174713 1105 14-15 0.10330573214761074 1105 16-17 0.150775472646977 1105 18-19 0.14766860628652267 1105 20-21 -0.026340560305975202 1105 22-23 -0.03134412159094779 1105 24-25 0.021936704173370458 1105 26-27 0.00737259747877772 1105 28-29 -0.15279168536884669 1105 30-31 -0.09961700309759891 1105 32-33 -0.0014164143766279835 1105 34-35 -0.09966491186584392 1105 36-37 -0.05988653875229488 1105 38-39 -0.23009986436488816 1105 40-41 -0.21520092960126647 1105 42-43 -0.1013214472923849 1105 44-45 -0.13649199461940498 1105 46-47 -0.06534145546829251 1105 48-49 0.05268411007846652 1105 50-51 0.11335228533947372 1105 52-53 0.25255458944366893 1105 54-55 0.12352779182212714 1105 56-57 0.20083410173632643 1105 58-59 0.3888945554003982 1105 60-61 0.2725558773043488 1105 62-63 0.23724301774291945 1105 64-65 -0.06946819931904002 1105 66-67 0.022640223349448263 1105 68-69 0.10258748773659221 1105 70-71 0.19142128431796834 1105 72-73 0.2642640750995575 1105 74-75 0.18513599787556245 1105 76-77 0.0501707608970996 1105 78-79 0.3741522843236993 1105 80-81 0.42399182173914696 1105 82-83 0.30835854620989167 1105 84-85 0.502250096621804 1105 86-87 0.5342491762035841 1105 88-89 0.3491901264667341 1105 90-91 0.42902202210777673 1105 92-93 0.4375076698225939 1105 94-95 0.49055972204245535 1105 96-97 0.4712989944270376 1105 98-99 -0.01286445593200014 1105 100-101 -0.21258249936925822 1106 1 0.10749474711806073 1106 2 0.18712351277007855 1106 3 0.15983385985085619 1106 4 0.12193487974715111 1106 5 0.15502156748458873 1106 6 0.03802280512680056 1106 7 0.05690467792477705 1106 8 0.05696003724199272 1106 9 0.10866605186846812 1106 10-11 0.05488299792973095 1106 12-13 0.05597470638006996 1106 14-15 0.18585371633093217 1106 16-17 0.18919269075414036 1106 18-19 0.1513220366936352 1106 20-21 0.06475724085142076 1106 22-23 -0.06095457761156098 1106 24-25 0.07152964235972092 1106 26-27 0.044299787081484965 1106 28-29 0.1269420536761814 1106 30-31 0.03926119732465594 1106 32-33 0.003709830478143772 1106 34-35 0.04533567037431396 1106 36-37 0.036366619267688804 1106 38-39 0.17687030975024243 1106 40-41 0.2036183379912444 1106 42-43 0.19608403994137547 1106 44-45 0.18256421940195366 1106 46-47 0.046839261750328376 1106 48-49 0.17010169778156126 1106 50-51 0.014680552339612518 1106 52-53 0.13074969558858385 1106 54-55 -0.02643828636006873 1106 56-57 -0.116819419804024 1106 58-59 0.04744791644498747 1106 60-61 -0.07323047599897592 1106 62-63 0.03755793372313576 1106 64-65 -0.005015732899103398 1106 66-67 -0.43955796107385936 1106 68-69 -0.310979214202014 1106 70-71 -0.14891682000934026 1106 72-73 -0.13474358515571794 1106 74-75 -0.18597957426399248 1106 76-77 -0.33363283684077416 1106 78-79 -0.40456455963087024 1106 80-81 -0.3421197759784711 1106 82-83 -0.2398686590879855 1106 84-85 -0.25914687471679443 1106 86-87 -0.2412433230243174 1106 88-89 -0.2166918661109669 1106 90-91 -0.07946526733646309 1106 92-93 -0.09014089001903969 1106 94-95 -0.2248152153034404 1106 96-97 0.12762591974334825 1106 98-99 -0.04469122176513807 1106 100-101 0.011138724043885162 1107 1 0.0043229417057375485 1107 2 0.033554767465794555 1107 3 0.004142578816271225 1107 4 0.026687588622664293 1107 5 -0.10762437470130948 1107 6 -0.018654897963877204 1107 7 -0.15941913589769996 1107 8 -0.014617695272796993 1107 9 -0.018049616901691934 1107 10-11 0.0030194229853677257 1107 12-13 0.026056665397703682 1107 14-15 0.12018280364056722 1107 16-17 0.13969332922888356 1107 18-19 0.03732187100444406 1107 20-21 0.05860103022045848 1107 22-23 0.002132154968933264 1107 24-25 0.11945381384231979 1107 26-27 0.0276505939592937 1107 28-29 -0.08819336867414762 1107 30-31 0.07753397316145794 1107 32-33 -0.06674378654556534 1107 34-35 -0.10460448154553603 1107 36-37 -0.02635673976651276 1107 38-39 0.013478194841923141 1107 40-41 0.14532287987490378 1107 42-43 0.2428835245908374 1107 44-45 0.1229489611410628 1107 46-47 0.17600141719535856 1107 48-49 -0.004521859222670344 1107 50-51 0.06264528910804756 1107 52-53 0.0029627604673336805 1107 54-55 0.0785086788137761 1107 56-57 0.17181744375663044 1107 58-59 0.09797140860188591 1107 60-61 0.18139468076299892 1107 62-63 -0.07599372845373153 1107 64-65 0.06265916204537803 1107 66-67 -0.011913004452999587 1107 68-69 -0.049126580457979685 1107 70-71 -0.04849574091560527 1107 72-73 0.2334426609270217 1107 74-75 0.18447547111485108 1107 76-77 0.058055564629864165 1107 78-79 0.08856562351282093 1107 80-81 0.15470499055688336 1107 82-83 0.2685243556840611 1107 84-85 0.3178323255757327 1107 86-87 0.3918082434527612 1107 88-89 0.26015106910169905 1107 90-91 0.28695524612864176 1107 92-93 0.01402247952785629 1107 94-95 0.12426649518039667 1107 96-97 0.13475598290520807 1107 98-99 0.2676020595379818 1107 100-101 -0.029898204649771998 1108 1 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2316 72-73 -1.453125239148111 2316 74-75 -1.554375486811523 2316 76-77 -1.642553939502367 2316 78-79 -1.5835474493647155 2316 80-81 -1.7275095098187734 2316 82-83 -1.6347997427381777 2316 84-85 -1.4279396503821893 2316 86-87 -1.9298911367125307 2316 88-89 -1.9518201931071673 2316 90-91 -2.3684838034581333 2316 92-93 -2.0819255858365295 2316 94-95 -2.2430804244289178 2316 96-97 -1.9867419509790913 2316 98-99 -1.7401688833620952 2316 100-101 -1.6888707816595385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 9.0 5 29.0 6 46.0 7 122.0 8 268.0 9 604.0 10 881.0 11 1527.0 12 2164.0 13 3165.0 14 4149.0 15 5789.0 16 7471.0 17 10032.0 18 8756.0 19 5831.0 20 5729.0 21 5720.0 22 6470.0 23 7048.0 24 8247.0 25 10263.0 26 12144.0 27 15417.0 28 19034.0 29 24014.0 30 29787.0 31 37881.0 32 48241.0 33 63590.0 34 87147.0 35 122785.0 36 180568.0 37 231146.0 38 122179.0 39 4364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82949353891162 19.24641841417055 14.44213671309692 22.481951333820916 2 16.01301954854459 20.451510328042463 39.524087171662345 24.0113829517506 3 20.332227069119853 24.020974476599125 29.431814645522557 26.21498380875846 4 13.345859844629771 16.76014263016467 36.8360887997247 33.057908725480864 5 14.077565044839888 37.46112320306364 33.90203138700696 14.559280365089505 6 32.95460993686723 35.99071221598034 16.858224923989905 14.196452923162534 7 29.660035785706768 32.345245122939005 19.908476453279516 18.086242638074708 8 26.91504034867257 34.9442294122008 19.008311359773604 19.13241887935303 9 26.52942430879256 17.058859600390623 19.852244656636316 36.5594714341805 10-11 25.413822580261353 26.531962680461056 27.00632792064564 21.047886818631948 12-13 26.46354901713133 23.659504053566753 27.709684445982035 22.16726248331988 14-15 22.891753171751414 25.498391929812357 25.922283624408987 25.687571274027242 16-17 22.49243070479566 28.78606627297789 26.576738192082534 22.144764830143917 18-19 22.22731202055282 27.28868171731728 28.949216982068908 21.53478928006099 20-21 23.090049683625548 26.23150821402079 29.056386361691484 21.622055740662184 22-23 22.663942303292117 26.245412361226787 28.94907606556777 22.141569269913326 24-25 22.75254572978197 26.41760951260006 29.09577398788593 21.734070769732035 26-27 22.577500888921225 26.60206028373176 29.06010480346947 21.760334023877544 28-29 22.16181684724213 26.995253601899293 29.08418129666544 21.75874825419314 30-31 22.412825790697696 26.48298433751811 29.003460517970257 22.10072935381394 32-33 22.317546875200215 26.81867356408947 28.887534299485075 21.976245261225245 34-35 22.8873236213936 26.62929122731229 28.68149297963495 21.80189217165916 36-37 22.867885190139358 26.35042899708175 28.857141157406623 21.92454465537227 38-39 22.46341759204809 26.70649639495958 28.974731538326008 21.855354474666324 40-41 22.641170107027342 26.73308512215614 28.751173786263816 21.874570984552697 42-43 22.60095624939365 26.811226777911813 28.399865641834815 22.18795133085973 44-45 22.82894026290691 26.708199320125136 28.314481481006887 22.14837893596106 46-47 23.072244828476194 27.2137197080773 27.75942735704519 21.95460810640132 48-49 23.25956115607021 27.12159458465053 27.575146577240666 22.04369768203859 50-51 22.868814799086223 27.308513208762886 27.733780093134957 22.088891899015934 52-53 23.137976322858464 27.464889279389354 27.266420468325986 22.130713929426193 54-55 22.680795448721234 27.331422933583255 27.48490778095873 22.502873836736786 56-57 22.964438020772786 27.592410710608 27.301469033498798 22.14168223512042 58-59 23.25476354240888 27.15378198061405 27.29560024730104 22.29585422967603 60-61 22.878944444393596 27.2035666005541 27.896375646290757 22.021113308761546 62-63 22.778155565948545 27.291310678856362 27.838803533168534 22.091730222026566 64-65 22.699176217060238 27.39900867807116 27.564396797859015 22.33741830700959 66-67 22.602689262217023 27.556174260677125 27.37600842756917 22.46512804953668 68-69 22.595475111465614 27.307897710189856 27.606771544794697 22.489855633549833 70-71 22.83920221203016 27.42380129071703 27.40471786272929 22.332278634523522 72-73 22.720880478680197 27.204228494285353 27.590559108538216 22.48433191849623 74-75 22.734455768256502 27.704820575624318 27.118432307233952 22.44229134888523 76-77 22.84930576561644 27.444921845147363 27.16275255547171 22.54301983376449 78-79 22.897377823278003 27.430091076215064 27.101592472265484 22.570938628241453 80-81 22.945908061872966 27.60459402330327 27.056952105243166 22.3925458095806 82-83 22.73712631711419 27.529968995618198 27.13055018476781 22.6023545024998 84-85 22.745344798392818 27.57856296248839 27.077599659526175 22.598492579592623 86-87 22.789795847389836 27.519599785468614 26.978878176556403 22.711726190585146 88-89 22.642004190040005 27.62355721399865 26.688349071152885 23.046089524808462 90-91 22.557383351219745 27.463599814194872 27.097572863298225 22.88144397128716 92-93 22.73506072726274 27.35468016364785 27.086281221684256 22.823977887405157 94-95 22.526873601405804 27.372908114935267 27.101266228268877 22.998952055390053 96-97 22.540134200915144 27.294734515481426 27.18829267935518 22.976838604248247 98-99 22.4628333126642 27.47765331044017 26.74884897330844 23.310664403587186 100-101 22.02494514219555 27.787131125597487 26.698441100222176 23.48948263198479 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 91.0 2 63.5 3 85.0 4 85.5 5 78.0 6 124.5 7 256.0 8 397.0 9 508.5 10 541.5 11 559.0 12 652.5 13 702.0 14 797.5 15 935.5 16 1058.0 17 1174.0 18 1246.0 19 1328.5 20 1452.5 21 1670.5 22 1971.0 23 2309.0 24 2684.5 25 3260.0 26 4196.0 27 5515.5 28 6996.5 29 8918.0 30 11247.0 31 13927.0 32 16654.0 33 18826.5 34 21304.5 35 23839.0 36 26611.5 37 29088.0 38 30905.5 39 32255.5 40 33245.0 41 35368.5 42 37575.0 43 39522.0 44 41117.0 45 41903.5 46 42399.5 47 44128.0 48 48226.0 49 47663.5 50 43387.0 51 40407.0 52 38041.5 53 36803.0 54 35308.0 55 32785.5 56 29588.5 57 26585.0 58 24276.5 59 21466.5 60 17650.5 61 14167.0 62 11346.0 63 8867.5 64 6619.5 65 4966.5 66 3699.0 67 2945.0 68 2241.5 69 1451.0 70 1045.0 71 808.5 72 634.0 73 510.0 74 416.0 75 350.5 76 247.5 77 154.5 78 107.5 79 72.0 80 49.0 81 35.0 82 25.0 83 15.0 84 9.0 85 5.0 86 3.0 87 2.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014186110790779577 2 0.009792992610409126 3 0.004759211362067987 4 1.8304659084876872E-4 5 0.005857490907160599 6 0.0 7 2.745698862731531E-4 8 0.0015558960222145342 9 9.152329542438436E-5 10-11 0.0 12-13 0.0 14-15 3.6609318169753745E-4 16-17 0.004210071589521682 18-19 0.0016931809653511108 20-21 4.1185482940972966E-4 22-23 7.321863633950749E-4 24-25 3.6609318169753745E-4 26-27 1.3728494313657656E-4 28-29 0.0 30-31 6.406630679706906E-4 32-33 0.0032948386352778373 34-35 0.001006756249668228 36-37 0.0010525178973804204 38-39 0.0046219264189314106 40-41 0.0 42-43 0.0014643727267901498 44-45 0.005857490907160599 46-47 0.0 48-49 0.0018304659084876875 50-51 9.152329542438438E-4 52-53 2.745698862731531E-4 54-55 1.8304659084876872E-4 56-57 0.011989551700594353 58-59 0.0020592741470486483 60-61 0.0033863619307022215 62-63 9.152329542438436E-5 64-65 0.0033406002829900294 66-67 0.0012355644882291888 68-69 0.0015101343745023421 70-71 0.004301594884946065 72-73 0.0027914605104437233 74-75 0.007550671872511711 76-77 0.0010525178973804204 78-79 0.006543915622843482 80-81 0.002242320737897417 82-83 0.0027914605104437233 84-85 0.002105035794760841 86-87 3.6609318169753745E-4 88-89 0.0024711289764583776 90-91 0.006910008804541019 92-93 0.0025626522718827623 94-95 2.745698862731531E-4 96-97 2.2880823856096094E-4 98-99 5.949014202584984E-4 100-101 0.0018762275561998794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1092618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.95210053698945 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13004078073328 53.994707664342215 2 10.200977381691715 13.251498169423911 3 2.8587988853473125 5.57054977848336 4 1.22951223339952 3.1943760878089624 5 0.6494923971905578 2.1092947740165697 6 0.4071421464443124 1.5866842577217994 7 0.28556558280606975 1.2983659111026677 8 0.19272611536162526 1.0014372816857366 9 0.15367304505557478 0.89832483650475 >10 0.8065545097227398 9.63629105190213 >50 0.056037819168237586 2.5216763526969532 >100 0.026658138471622822 3.1128549210803125 >500 0.0022567716859984593 0.9280476764793572 >1k 5.641929214996148E-4 0.8958912367513953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.26651583627580727 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2757 0.2523297254850277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.24271977946546736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.13344096472875241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.152329542438436E-5 0.0 0.0 0.0 0.0 3 9.152329542438436E-5 0.0 0.0 0.0 0.0 4 9.152329542438436E-5 0.0 0.0 0.0 0.0 5 9.152329542438436E-5 0.0 0.0 0.0 0.0 6 2.745698862731531E-4 0.0 0.0 0.0 0.0 7 2.745698862731531E-4 0.0 0.0 0.0 0.0 8 2.745698862731531E-4 0.0 0.0 0.0 0.0 9 2.745698862731531E-4 0.0 0.0 0.0 0.0 10-11 2.745698862731531E-4 0.0 0.0 0.0 0.0 12-13 2.745698862731531E-4 0.0 0.0 0.0 0.0 14-15 2.745698862731531E-4 0.0 0.0 0.0 0.0 16-17 3.203315339853453E-4 0.0 0.0 9.152329542438436E-5 0.0 18-19 3.6609318169753745E-4 0.0 0.0 9.152329542438436E-5 0.0 20-21 3.6609318169753745E-4 0.0 0.0 1.8304659084876872E-4 0.0 22-23 3.6609318169753745E-4 0.0 0.0 4.118548294097296E-4 0.0 24-25 5.491397725463061E-4 0.0 0.0 5.949014202584985E-4 0.0 26-27 6.406630679706906E-4 0.0 0.0 0.0010525178973804202 0.0 28-29 6.406630679706906E-4 0.0 0.0 0.002242320737897417 0.0 30-31 6.406630679706906E-4 0.0 0.0 0.004804973009780179 0.0 32-33 6.406630679706906E-4 0.0 0.0 0.007276101986238558 9.152329542438436E-5 34-35 8.237096588194592E-4 0.0 0.0 0.009792992610409128 9.152329542438436E-5 36-37 9.152329542438436E-4 0.0 0.0 0.014964058801886844 9.152329542438436E-5 38-39 0.001006756249668228 0.0 0.0 0.021645259367866904 9.152329542438436E-5 40-41 0.001006756249668228 0.0 0.0 0.03711269629458786 9.152329542438436E-5 42-43 0.0010525178973804202 0.0 0.0 0.049788672710865095 9.152329542438436E-5 44-45 0.001189802840516997 0.0 0.0 0.06484425480817632 9.152329542438436E-5 46-47 0.0013270877836535733 0.0 0.0 0.09522998888907194 9.152329542438436E-5 48-49 0.0013728494313657656 0.0 0.0 0.11326007808767566 9.152329542438436E-5 50-51 0.0013728494313657656 0.0 0.0 0.13485957580783037 9.152329542438436E-5 52-53 0.0013728494313657656 0.0 0.0 0.16016576699267265 9.152329542438436E-5 54-55 0.0014643727267901498 0.0 0.0 0.1920158738003584 9.152329542438436E-5 56-57 0.0015558960222145342 0.0 0.0 0.28244088967965014 9.152329542438436E-5 58-59 0.0015558960222145342 0.0 0.0 0.363118674596245 9.152329542438436E-5 60-61 0.0015558960222145342 0.0 0.0 0.41986311775936325 9.152329542438436E-5 62-63 0.0015558960222145342 0.0 0.0 0.48914625239562226 9.152329542438436E-5 64-65 0.0015558960222145342 0.0 0.0 0.5666207219723636 9.152329542438436E-5 66-67 0.0015558960222145342 0.0 0.0 0.6910008804541019 9.152329542438436E-5 68-69 0.0016474193176389186 0.0 0.0 0.8428837892108678 9.152329542438436E-5 70-71 0.001693180965351111 0.0 0.0 0.9276343607738478 9.152329542438436E-5 72-73 0.0017847042607754952 0.0 0.0 1.0199356042093395 9.152329542438436E-5 74-75 0.0018304659084876873 0.0 0.0 1.1838538263144116 9.152329542438436E-5 76-77 0.0018304659084876873 0.0 0.0 1.3841983199983892 9.152329542438436E-5 78-79 0.0018304659084876873 0.0 0.0 1.572232930447787 9.152329542438436E-5 80-81 0.0019219892039120717 0.0 0.0 1.7075501227327392 9.152329542438436E-5 82-83 0.0019219892039120717 0.0 0.0 1.9169096610160183 9.152329542438436E-5 84-85 0.0019219892039120717 0.0 0.0 2.2579712214149867 9.152329542438436E-5 86-87 0.0019219892039120717 0.0 0.0 2.5977514556780137 9.152329542438436E-5 88-89 0.0019219892039120717 0.0 0.0 3.0041606490099926 9.152329542438436E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 90 8.9396385E-4 26.392742 1 GTACATG 4190 0.0 25.624266 1 TACCTGG 950 0.0 25.500221 2 TACATGG 4245 0.0 24.505512 2 GTGTAGG 565 0.0 24.38409 1 ACATGGG 4210 0.0 23.578806 3 GTAAGGT 425 0.0 23.468643 4 GTATCAA 7190 0.0 23.125769 1 GTACCTG 1080 0.0 22.43383 1 GAGTACT 3920 0.0 22.235016 12-13 GGTATCA 5520 0.0 21.429756 1 GTACTTT 4155 0.0 20.692118 14-15 TCAACGC 8200 0.0 20.156868 4 ATCAACG 8225 0.0 20.095598 3 CAACGCA 8270 0.0 20.043684 5 ATACGTC 190 8.256544E-6 19.998344 5 TATCAAC 8325 0.0 19.913086 2 AACGCAG 8490 0.0 19.524296 6 TTGCGTA 195 0.0 19.486458 60-61 CATGGGA 2000 0.0 18.760946 4 >>END_MODULE