FastQCFastQC Report
Thu 26 May 2016
SRR936534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316203
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.21600011548370576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21490.16327268666003647No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20180.15331981464865224No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14850.1128245415030964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG49000.025.607561
TACCTGG11400.025.0134562
TACATGG50050.024.6886082
TGCACCG3400.023.7636895
TGTACGC1201.7047672E-423.7627872
ACATGGG49800.023.285623
GCACCGT4300.023.2110466
GTATCAA68400.023.2086961
GTACCTG12300.023.1849671
CTAGTAC1954.200574E-721.934883
GGTATCA51850.021.2666761
CATGGGG26250.021.0017764
GAGTACT34150.020.87070312-13
ATCAACG78500.020.0394443
TCAACGC78650.019.9407984
TATCAAC79450.019.799832
CAACGCA79050.019.7805275
GTACTTT36350.019.53922314-15
GTGTACG1951.0273694E-519.4991551
AACGCAG80950.019.3749666
Produced by FastQC (version 0.11.5)