FastQCFastQC Report
Thu 26 May 2016
SRR936534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316203
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.2542161049625324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.23058753095077278No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29660.22534517851729557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.128551598803528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG51700.026.4260861
TCGCGCT2050.025.48669260-61
TACATGG53900.024.5889282
GTACCGG1601.6654576E-624.0533221
ACATGGG54150.023.5077463
ATTCGCG2300.022.716480-81
GTATCAA83700.022.415281
TACCTGG11150.021.727992
GAGTACT44850.021.70453812-13
GTACCTG11550.021.2418941
GTACCGT1353.8033168E-421.105386
GTACACG1604.5251807E-521.0466561
CAATTCG2500.020.89908878-79
GTACTTT46550.020.70787414-15
AATTCGC2450.020.35625578-79
CGATCGT2450.020.35084324-25
CGCGCTT2600.020.0952862-63
CGCGGAA2400.019.79193382-83
CTCGCGC2650.019.71612460-61
TATCAAC94700.019.5631522
Produced by FastQC (version 0.11.5)