##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936534_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1316203 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.606788618472986 31.0 31.0 34.0 30.0 34.0 2 31.875016999657348 33.0 31.0 34.0 30.0 34.0 3 32.17326810529987 33.0 31.0 34.0 30.0 34.0 4 35.939989500099905 37.0 35.0 37.0 35.0 37.0 5 35.780104588729856 37.0 35.0 37.0 35.0 37.0 6 35.88525250284341 37.0 35.0 37.0 35.0 37.0 7 35.82815416770817 37.0 35.0 37.0 35.0 37.0 8 35.79867087371781 37.0 35.0 37.0 33.0 37.0 9 37.47731922811299 39.0 37.0 39.0 35.0 39.0 10-11 37.364195720568944 39.0 37.0 39.0 34.0 39.0 12-13 37.24725441288312 39.0 37.0 39.0 33.5 39.0 14-15 38.44121689435444 40.0 38.0 41.0 34.0 41.0 16-17 38.357387120375805 40.0 38.0 41.0 33.5 41.0 18-19 38.38823646504376 40.0 38.0 41.0 33.5 41.0 20-21 38.448555807880695 40.0 38.0 41.0 34.0 41.0 22-23 38.36932600822213 40.0 38.0 41.0 34.0 41.0 24-25 38.27289863341748 40.0 38.0 41.0 34.0 41.0 26-27 38.08921268223823 40.0 37.5 41.0 33.0 41.0 28-29 38.0256795494312 40.0 37.5 41.0 33.0 41.0 30-31 38.04931496129396 40.0 37.0 41.0 33.0 41.0 32-33 37.91502564574006 40.0 37.0 41.0 33.0 41.0 34-35 37.775114856902775 40.0 37.0 41.0 33.0 41.0 36-37 37.57706637957823 40.0 37.0 41.0 33.0 41.0 38-39 37.44036368250186 40.0 37.0 41.0 32.0 41.0 40-41 37.22207630585859 39.5 36.0 41.0 31.5 41.0 42-43 37.01311043965103 39.0 36.0 41.0 31.0 41.0 44-45 36.77862229458526 39.0 36.0 41.0 31.0 41.0 46-47 36.43537736960028 39.0 35.5 40.5 30.0 41.0 48-49 35.99898457912647 39.0 35.0 40.0 29.0 41.0 50-51 35.85214628746478 39.0 35.0 40.0 29.0 41.0 52-53 35.600909206254656 38.0 35.0 40.0 28.0 41.0 54-55 35.33703881544108 38.0 34.5 40.0 28.0 41.0 56-57 35.071328282947235 38.0 34.0 40.0 27.0 41.0 58-59 34.69391271711127 38.0 34.0 40.0 26.5 41.0 60-61 34.32856937721613 37.0 33.5 40.0 26.0 41.0 62-63 34.04455201819172 37.0 33.0 39.5 26.0 41.0 64-65 33.61041913747347 36.0 33.0 39.0 25.0 40.0 66-67 33.21853885760783 36.0 32.0 39.0 24.0 40.0 68-69 32.76297235304888 35.0 32.0 38.5 23.0 40.0 70-71 32.3008806392327 35.0 31.0 38.0 22.0 40.0 72-73 31.87335502198369 35.0 31.0 37.0 21.5 39.0 74-75 31.49101012533781 34.5 31.0 37.0 20.0 39.0 76-77 30.07701357617328 33.5 29.5 35.0 19.5 37.5 78-79 30.872781402260898 34.0 30.5 36.0 20.0 38.0 80-81 30.70844504988972 34.0 30.5 35.5 18.5 37.0 82-83 30.37091960738579 34.0 30.0 35.0 18.0 37.0 84-85 29.92049060821165 34.0 30.0 35.0 14.5 36.0 86-87 29.54047665899561 34.0 29.0 35.0 8.5 36.0 88-89 29.139475065776328 34.0 29.0 35.0 6.5 35.5 90-91 28.702475985847165 33.5 29.0 35.0 2.0 35.0 92-93 28.26891938401599 33.0 29.0 35.0 2.0 35.0 94-95 27.74793136013214 33.0 27.0 35.0 2.0 35.0 96-97 27.062572794622106 33.0 26.0 34.0 2.0 35.0 98-99 26.19592988315632 32.0 25.0 34.0 2.0 35.0 100-101 23.34299154461736 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2659371108482347 1101 2 0.2691359307373169 1101 3 0.16050143835283137 1101 4 0.07244312017660803 1101 5 0.12382220321099169 1101 6 0.10330675503487186 1101 7 0.17457096024892138 1101 8 0.0646688669911768 1101 9 0.04980748914573496 1101 10-11 0.112671750741562 1101 12-13 0.14227366527629925 1101 14-15 0.1230152951777157 1101 16-17 0.08027193727495785 1101 18-19 0.0780611842029515 1101 20-21 1.9542804004402115E-4 1101 22-23 -0.10764574583073028 1101 24-25 -0.10920314682109478 1101 26-27 -0.05869140409061657 1101 28-29 -0.03037161875932526 1101 30-31 -0.14676979953982539 1101 32-33 -0.07727686300752623 1101 34-35 0.0125438061159997 1101 36-37 -0.23212574611300596 1101 38-39 -0.1967327964540999 1101 40-41 -0.04417886953795147 1101 42-43 -0.042017470632501386 1101 44-45 -0.2137682787989803 1101 46-47 0.034315680349322975 1101 48-49 0.15754918291332842 1101 50-51 -0.08940915128523841 1101 52-53 -0.05948554854099797 1101 54-55 -0.10770136173059086 1101 56-57 -0.19878681402590104 1101 58-59 -0.2688611957485918 1101 60-61 -0.30127638084392316 1101 62-63 -0.4858794833420248 1101 64-65 -0.5361014664283772 1101 66-67 -0.6887479250322812 1101 68-69 -0.5968364261258472 1101 70-71 -0.6271420948199413 1101 72-73 -0.49453829993456466 1101 74-75 -0.4849529140496074 1101 76-77 -0.13034147016326614 1101 78-79 -0.2064581889752155 1101 80-81 -0.3044794560804327 1101 82-83 -0.1433761883571556 1101 84-85 -0.14745615915671806 1101 86-87 -0.4479618981689981 1101 88-89 -0.5876088754301136 1101 90-91 -0.640436254882367 1101 92-93 -0.4025323692220084 1101 94-95 -0.5178244660885625 1101 96-97 -0.2708929061317278 1101 98-99 -0.4773639229965134 1101 100-101 -0.28830387907322574 1102 1 0.41926987331459387 1102 2 0.4768899051879636 1102 3 0.11250974731109409 1102 4 0.030131134213611688 1102 5 0.15303563594258662 1102 6 0.04713628867973796 1102 7 0.0075143240309500925 1102 8 -0.13429175487886624 1102 9 -0.0027263860688506725 1102 10-11 0.06235003771090675 1102 12-13 0.11819724535793341 1102 14-15 0.15808790850876164 1102 16-17 0.3285718964821953 1102 18-19 0.18462556456844936 1102 20-21 0.07450034747038359 1102 22-23 0.09623339004519949 1102 24-25 0.1076325101619986 1102 26-27 -0.17853871086767725 1102 28-29 -0.07236336098950602 1102 30-31 0.10536723886762189 1102 32-33 0.18854155010657792 1102 34-35 0.27109409259748674 1102 36-37 0.27772502694304535 1102 38-39 0.3324593077605158 1102 40-41 0.47023604816399 1102 42-43 0.3222919262065176 1102 44-45 0.27785798744347545 1102 46-47 0.18845418679070036 1102 48-49 0.29271147080912385 1102 50-51 0.35271252111064655 1102 52-53 0.2358257798717105 1102 54-55 0.35294341671667695 1102 56-57 0.34418556601857375 1102 58-59 0.33476774872363535 1102 60-61 0.36277830610558937 1102 62-63 0.26530124755939255 1102 64-65 0.300197900004072 1102 66-67 0.23472124830916385 1102 68-69 0.17650337001144578 1102 70-71 0.09556933160915548 1102 72-73 -0.05152477955066459 1102 74-75 -0.4226050772193979 1102 76-77 0.002035945370280956 1102 78-79 -0.02272897203512869 1102 80-81 0.0524640189258605 1102 82-83 0.2568523269537195 1102 84-85 0.3288886196381213 1102 86-87 0.12336745615833422 1102 88-89 0.1335575764241561 1102 90-91 0.03796146476739182 1102 92-93 0.08665043891897639 1102 94-95 -0.12039899187663394 1102 96-97 -0.12054202832896621 1102 98-99 -0.31651483315542706 1102 100-101 -0.40458379389819044 1103 1 0.4314967965237919 1103 2 0.4243739200287031 1103 3 0.24719136356634408 1103 4 0.13635558311012375 1103 5 0.019782422598524363 1103 6 -0.045555296182143934 1103 7 -0.06258059533531934 1103 8 0.11429117236355069 1103 9 0.05312243207559675 1103 10-11 0.06384015379918395 1103 12-13 0.16516772299542737 1103 14-15 0.15754132904195473 1103 16-17 0.1154758138868246 1103 18-19 0.1227224266141036 1103 20-21 0.14643010887503038 1103 22-23 0.13926836695257805 1103 24-25 0.1284634395206652 1103 26-27 0.1100698723424145 1103 28-29 0.16327345953475714 1103 30-31 0.314241593731893 1103 32-33 -0.053530636953780686 1103 34-35 0.05214457944052242 1103 36-37 0.04052287739595073 1103 38-39 0.15435893577790694 1103 40-41 0.07799288024661166 1103 42-43 0.15935290686104508 1103 44-45 0.12134501337298076 1103 46-47 0.34493582213845997 1103 48-49 0.475001428243111 1103 50-51 0.22961932167775956 1103 52-53 0.2510170248289896 1103 54-55 0.23049627710636145 1103 56-57 0.31400219752693914 1103 58-59 0.2838905376963936 1103 60-61 0.20827374238400154 1103 62-63 0.1768215870332952 1103 64-65 0.33897000706229363 1103 66-67 0.17342961722667383 1103 68-69 0.11759685679631104 1103 70-71 -0.0339327169790451 1103 72-73 0.07410203508191415 1103 74-75 0.0031118643655467793 1103 76-77 0.006980713683905293 1103 78-79 0.07162469011158379 1103 80-81 0.29924183573865193 1103 82-83 0.28582216367415114 1103 84-85 0.294572718241632 1103 86-87 0.16458237647601948 1103 88-89 0.10711637285006148 1103 90-91 0.07000667481236889 1103 92-93 0.17558041278408965 1103 94-95 0.1661222716878079 1103 96-97 -0.04912059707825023 1103 98-99 -0.1911821203823756 1103 100-101 -0.09496074891217887 1104 1 0.19510635750989636 1104 2 0.23111799989835546 1104 3 -0.011846941275628353 1104 4 -0.08395659739601058 1104 5 -0.2202152894614855 1104 6 -0.04914544392894982 1104 7 -0.08408140861578062 1104 8 -0.1246197682024146 1104 9 -0.0788249165341881 1104 10-11 -0.013933022981511556 1104 12-13 -0.09806217010503104 1104 14-15 -0.036250784209705955 1104 16-17 -0.16842106167081994 1104 18-19 -0.1497068347683097 1104 20-21 -0.23655888606512576 1104 22-23 -0.14607782268544867 1104 24-25 -0.14984938969180917 1104 26-27 -0.12369919710649668 1104 28-29 -0.22459162187720239 1104 30-31 -0.27506718466228364 1104 32-33 -0.1215391393223797 1104 34-35 -0.1345477272242661 1104 36-37 -0.07782167472095836 1104 38-39 -0.12214392055076218 1104 40-41 -0.08433200928664064 1104 42-43 -0.3520936431960351 1104 44-45 -0.2624935676658211 1104 46-47 -0.18005067456304147 1104 48-49 -0.29472015206580693 1104 50-51 -0.35199540224357406 1104 52-53 -0.3086211449971259 1104 54-55 -0.15295287462618745 1104 56-57 -0.06992629133322481 1104 58-59 -0.0663560837389312 1104 60-61 -0.34095207421021456 1104 62-63 -0.6097180647134692 1104 64-65 -0.4033456142589813 1104 66-67 -0.20091641040602326 1104 68-69 -0.2313054286433882 1104 70-71 -0.21459811705857135 1104 72-73 -0.2620742691894442 1104 74-75 -0.09404149988237975 1104 76-77 0.005224514239927203 1104 78-79 0.13244810190276368 1104 80-81 0.12852400826782429 1104 82-83 0.1386160021284617 1104 84-85 0.12485141193750238 1104 86-87 0.2348510758472102 1104 88-89 0.2404286078146498 1104 90-91 0.12831403762929838 1104 92-93 0.06307027888822248 1104 94-95 0.11989406748436693 1104 96-97 0.10948724624033446 1104 98-99 0.15024086183779772 1104 100-101 0.21305522693704404 1105 1 0.3043867140362764 1105 2 0.3511004246543443 1105 3 0.16403103631593297 1105 4 0.1352788794893982 1105 5 -0.015024315604662775 1105 6 0.09086020525662519 1105 7 0.0962217643434542 1105 8 0.04934376316625588 1105 9 0.043966408827515124 1105 10-11 0.07674425160028875 1105 12-13 -0.011151019240394078 1105 14-15 0.06229611972275961 1105 16-17 0.11964817223684321 1105 18-19 0.02636729510018654 1105 20-21 0.0718081854598367 1105 22-23 0.007352488333602025 1105 24-25 -0.09803380421649166 1105 26-27 -0.12360504401356565 1105 28-29 -0.05910186887215474 1105 30-31 -0.004306427671423307 1105 32-33 -0.018425810382851182 1105 34-35 0.01835689569259813 1105 36-37 0.08973944632852948 1105 38-39 0.04686872734805547 1105 40-41 0.00948370172612556 1105 42-43 -0.07827291068497289 1105 44-45 -0.16836401300995618 1105 46-47 -0.02344269665763221 1105 48-49 0.07390489893378316 1105 50-51 -0.02982705651341888 1105 52-53 -0.15183270699693452 1105 54-55 -0.06473142655162434 1105 56-57 -0.10203249602204778 1105 58-59 -0.08705407200118742 1105 60-61 -0.022290931191669472 1105 62-63 -0.22045779418024836 1105 64-65 -0.11898758143092891 1105 66-67 -0.10371118804780366 1105 68-69 -0.46741000134527155 1105 70-71 -0.31701314389220414 1105 72-73 -0.34418361231469774 1105 74-75 -0.40665330668023003 1105 76-77 -0.5139670245354537 1105 78-79 -0.3102832793231123 1105 80-81 -0.4058811011066865 1105 82-83 -0.03847811958010183 1105 84-85 -0.05516930174294288 1105 86-87 -0.10502589744469759 1105 88-89 -0.045161261312550494 1105 90-91 -0.3000197664694966 1105 92-93 -0.07258078974938442 1105 94-95 0.08681494750194219 1105 96-97 0.021956254180580004 1105 98-99 0.25257742776065584 1105 100-101 0.3421861938893045 1106 1 0.2908587187482219 1106 2 0.3493138363592472 1106 3 0.13341619805534322 1106 4 -0.0024758262363562267 1106 5 -0.18202544909652119 1106 6 -0.06968537914025319 1106 7 -0.1842371527985236 1106 8 0.03341152833208838 1106 9 0.043567084850202775 1106 10-11 -0.055193106948898674 1106 12-13 0.08657569987006752 1106 14-15 0.16553437699599982 1106 16-17 0.11364454928616397 1106 18-19 -0.07234397066254417 1106 20-21 -0.03052961321300529 1106 22-23 0.07869365882419999 1106 24-25 -0.011768889899578028 1106 26-27 -0.0441836904749735 1106 28-29 0.018142245152752423 1106 30-31 -0.060594328569685274 1106 32-33 0.04261721328774115 1106 34-35 -0.18109242183670915 1106 36-37 -0.07451996418028983 1106 38-39 0.05578526599725819 1106 40-41 0.14603986990525186 1106 42-43 -0.0496842928135095 1106 44-45 -0.11385101103633843 1106 46-47 -0.25149514950233254 1106 48-49 -0.22928003972718614 1106 50-51 -0.200490922256094 1106 52-53 -0.04626769939130071 1106 54-55 -0.15412343599964373 1106 56-57 -0.31882544559712045 1106 58-59 -0.18080546247065143 1106 60-61 -0.19770765724786799 1106 62-63 -0.49124281459139496 1106 64-65 -0.26534943456568527 1106 66-67 -0.13127076424402162 1106 68-69 -0.2031910134335817 1106 70-71 -0.2924928040105641 1106 72-73 -0.03991891519382662 1106 74-75 -0.01443175314027556 1106 76-77 0.01573641990723118 1106 78-79 0.05540378039298233 1106 80-81 -0.12845444436467446 1106 82-83 -0.03165548654563821 1106 84-85 0.0018906632191146855 1106 86-87 -0.07700157450434375 1106 88-89 -0.1926311536421359 1106 90-91 -0.18456423170342617 1106 92-93 0.13678092537537978 1106 94-95 0.15802659222653048 1106 96-97 -0.024443382016045234 1106 98-99 -0.16570048664449288 1106 100-101 -0.1628707785939163 1107 1 0.6706460794158993 1107 2 0.5986049218910914 1107 3 0.425429445128664 1107 4 0.2169883394500829 1107 5 0.16879444111800979 1107 6 0.2559041576322798 1107 7 0.2285541147755552 1107 8 0.2854892603659209 1107 9 0.21273333141940753 1107 10-11 0.3668919417824057 1107 12-13 0.3580680610978746 1107 14-15 0.4601688571954057 1107 16-17 0.4808342372937844 1107 18-19 0.37817117385097987 1107 20-21 0.24372838964365684 1107 22-23 0.4287302423115662 1107 24-25 0.5821141409635757 1107 26-27 0.36268070206409675 1107 28-29 0.27399385272571664 1107 30-31 0.24910764728871015 1107 32-33 0.29502927288557146 1107 34-35 0.43388650327391787 1107 36-37 0.5446923180691883 1107 38-39 0.5343042824090674 1107 40-41 0.6529084634189459 1107 42-43 0.723019349431496 1107 44-45 0.7169941200340659 1107 46-47 0.8221033003380356 1107 48-49 0.9177864546995167 1107 50-51 1.0405286192775378 1107 52-53 0.6390075344629551 1107 54-55 0.8443502098861941 1107 56-57 0.8568461852212224 1107 58-59 0.648946539993922 1107 60-61 0.7682670738897528 1107 62-63 0.8282850658768766 1107 64-65 1.0001862947235125 1107 66-67 0.9872002210245583 1107 68-69 0.8674081155643449 1107 70-71 0.9798948899344353 1107 72-73 1.1890797124877928 1107 74-75 0.983255960409263 1107 76-77 0.8250156726938798 1107 78-79 1.1182494855965892 1107 80-81 0.901607029897356 1107 82-83 0.875801418456323 1107 84-85 1.0017445043102455 1107 86-87 1.1800936965985507 1107 88-89 1.1532184584678724 1107 90-91 1.144681028196871 1107 92-93 1.306462666539332 1107 94-95 1.190182427380286 1107 96-97 1.0967048493736336 1107 98-99 1.2668248592357507 1107 100-101 1.169285406641059 1108 1 0.23169748909846177 1108 2 0.27391007443521076 1108 3 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-1.3017629887441942 2316 92-93 -1.6470948078104648 2316 94-95 -1.900543980398286 2316 96-97 -1.99186664311026 2316 98-99 -2.2775410438899932 2316 100-101 -2.5243326141177107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 39.0 6 65.0 7 185.0 8 386.0 9 785.0 10 1248.0 11 1939.0 12 2924.0 13 4046.0 14 5231.0 15 7051.0 16 9031.0 17 11276.0 18 9898.0 19 6783.0 20 6530.0 21 6689.0 22 7295.0 23 8127.0 24 9664.0 25 11762.0 26 14338.0 27 18159.0 28 22613.0 29 28447.0 30 35860.0 31 45661.0 32 57978.0 33 75140.0 34 100989.0 35 139993.0 36 204242.0 37 275746.0 38 175836.0 39 10236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.687432874494924 19.218205206323415 14.475874824204265 22.618487094977397 2 15.870981174053384 20.463035007628854 39.55811910739361 24.107864710924154 3 20.197592660974273 23.946580232224182 29.563603319398666 26.29222378740288 4 13.404871777141688 16.73526951153812 36.587567599194244 33.27229111212595 5 14.023216783429305 37.3652088621588 33.97750954829916 14.634064806112734 6 32.98890824591647 35.86931499168441 16.943966850098352 14.197809912300762 7 29.720111563337877 32.2622726129632 19.919343748646675 18.09827207505225 8 26.93953744217267 34.91991736836947 19.057394642011907 19.08315054744595 9 26.52697609005681 16.97355994429194 19.84223646750705 36.65722749814421 10-11 25.44558299403509 26.48539694285886 27.014724999373197 21.054295063732862 12-13 26.403712208844038 23.619517587596086 27.73227422176383 22.244495981796046 14-15 22.926124913244667 25.417498764436903 25.875141458203778 25.781234864114648 16-17 22.57391147110286 28.664727249520023 26.52106096096119 22.24030031841593 18-19 22.292116033772906 27.274896045670765 28.853527913247422 21.579460007308903 20-21 23.011343994809312 26.158996750501252 29.06527336588657 21.764385888802867 22-23 22.67633702505799 26.18013245838167 28.92165019104428 22.221880325516054 24-25 22.845754036421432 26.38791280676309 28.993172025895703 21.773161130919775 26-27 22.572590463853395 26.590171345214735 28.96985836146034 21.867379829471528 28-29 22.17078216658069 26.877692878682087 29.046355311452714 21.90516964328451 30-31 22.41962100042395 26.44875178733963 28.980506031748927 22.151121180487493 32-33 22.33341995131158 26.696002808246032 28.867112825235314 22.10346441520708 34-35 22.94515318042263 26.51047376790486 28.61734563244144 21.92702741923107 36-37 22.92283203726005 26.311414688999395 28.756871341141434 22.008881932599124 38-39 22.551786412318194 26.57009822599082 28.881867832346085 21.996247529344906 40-41 22.717257099439642 26.601690568904928 28.690435938835595 21.99061639281983 42-43 22.719886445020173 26.65882480778137 28.344824468119462 22.276464279078994 44-45 22.85460844723706 26.613621340708267 28.247566803680375 22.2842034083743 46-47 23.0844331356532 27.04903989451319 27.770633806000454 22.095893163833157 48-49 23.266965238244314 27.045106394619022 27.54349398652325 22.144434380613415 50-51 22.905046184353733 27.13751977769209 27.713575331875845 22.243858706078328 52-53 23.18992096693558 27.317768839303486 27.299799872051832 22.192510321709097 54-55 22.81582261768846 27.097534056089252 27.47355203443697 22.613091291785317 56-57 22.993681943944775 27.402151049064756 27.321385086865195 22.282781920125274 58-59 23.31098330015651 27.065484964062662 27.25592244221915 22.36760929356167 60-61 22.974428449693658 27.084856941937545 27.897528401330757 22.043186207038037 62-63 22.815556664023816 27.13347954477159 27.83519567398522 22.21576811721938 64-65 22.75535331682038 27.24215796126941 27.522756607176714 22.4797321147335 66-67 22.743792849444617 27.397507984380358 27.317540715876103 22.54115845029892 68-69 22.678041833292117 27.116347395400624 27.569051979542632 22.63655879176463 70-71 22.85770291672191 27.29985454189053 27.390647146130124 22.451795395257435 72-73 22.836759541180022 27.17389611400981 27.455615682237344 22.53372866257282 74-75 22.814375897942796 27.545295064667073 27.093085185188 22.547243852202133 76-77 22.832317900116017 27.291144799333228 27.20498682937911 22.67155047117165 78-79 22.921326372475306 27.27766293282311 27.136062333138682 22.664948361562903 80-81 23.02777558253342 27.45437606764865 27.078685325568248 22.439163024249684 82-83 22.84602586543273 27.43598326296245 27.109274405425214 22.60871646617961 84-85 22.877539041352204 27.41243358553575 27.11947860015311 22.590548772958936 86-87 22.84604024739454 27.45568033689061 27.027918319832555 22.670361095882292 88-89 22.767912107287962 27.48809456856966 26.730714569086462 23.013278755055914 90-91 22.740166396558834 27.29956130434535 27.11435536057971 22.845916938516112 92-93 22.802594809469323 27.159947909306275 27.21518410291711 22.822273178307295 94-95 22.58403014147146 27.198027750244 27.2054742164209 23.012467891863633 96-97 22.638765359959606 27.117226748203993 27.241310792538393 23.002697099298008 98-99 22.50716071653137 27.317065016152693 26.827108255900583 23.348666011415354 100-101 22.042352197396376 27.57820597380571 26.907619383409877 23.471822445388035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 121.0 1 90.0 2 72.5 3 96.5 4 93.0 5 94.5 6 169.5 7 317.0 8 459.0 9 600.5 10 676.0 11 689.5 12 746.0 13 844.0 14 980.0 15 1061.5 16 1133.0 17 1297.5 18 1419.5 19 1498.5 20 1727.0 21 1992.5 22 2300.5 23 2655.0 24 3226.5 25 4043.5 26 4998.0 27 6650.0 28 8499.0 29 10711.5 30 13535.0 31 16532.0 32 19857.0 33 22557.0 34 25294.0 35 28301.0 36 31582.5 37 34527.0 38 36813.5 39 38286.5 40 39626.0 41 42439.0 42 45033.5 43 47182.5 44 48984.0 45 50120.0 46 50865.5 47 52889.0 48 57853.0 49 57394.0 50 52254.0 51 48985.5 52 46150.0 53 44544.5 54 43086.0 55 40219.0 56 36246.0 57 32359.5 58 29493.5 59 26237.0 60 21733.0 61 17415.5 62 14062.5 63 11189.0 64 8389.5 65 6213.5 66 4670.0 67 3851.5 68 2851.0 69 1797.0 70 1285.0 71 999.5 72 819.5 73 645.5 74 509.5 75 409.5 76 305.5 77 201.5 78 128.0 79 90.5 80 61.5 81 43.0 82 32.5 83 19.5 84 13.0 85 3.5 86 3.5 87 5.5 88 3.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2459324283564162 2 0.01116848996697318 3 0.004634543455682748 4 0.01321984526702948 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004254662844561211 10-11 4.5585673334584405E-4 12-13 4.1786867223369043E-4 14-15 1.1396418333646101E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012156179555889175 24-25 0.0 26-27 3.798806111215367E-5 28-29 0.0 30-31 7.597612222430734E-5 32-33 0.00600211365572028 34-35 7.597612222430734E-4 36-37 0.0036468538667667524 38-39 2.659164277850757E-4 40-41 0.005926137533495973 42-43 0.014321499039281933 44-45 0.002507212033402142 46-47 0.0014815343833739932 48-49 0.012080203433664867 50-51 7.977492833552271E-4 52-53 0.0034189255000938307 54-55 0.01333380945036594 56-57 0.00414069866122475 58-59 0.0017474508111590692 60-61 0.008471337628010268 62-63 0.005508268861262283 64-65 0.020247636572777908 66-67 0.00300105682786014 68-69 1.8994030556076835E-4 70-71 0.001253606016701071 72-73 3.798806111215367E-5 74-75 0.0 76-77 0.0012156179555889175 78-79 0.013485761694814554 80-81 0.017246579744917768 82-83 0.015157236383749316 84-85 0.009914883950272109 86-87 0.01272600047257148 88-89 0.03168204296753616 90-91 0.02814915328410587 92-93 0.0028111165222993715 94-95 0.010826597416963797 96-97 0.012118191494777022 98-99 0.012801976594795789 100-101 0.014511439344842702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1316203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.78524254997653 #Duplication Level Percentage of deduplicated Percentage of total 1 82.30496000295007 52.49841836854288 2 10.622380405865089 13.551022212924458 3 3.1022486905396884 5.936330555292633 4 1.2413773637389176 3.1672622496854914 5 0.6956224777531167 2.2185224233349103 6 0.42579739788276094 1.6295754180660462 7 0.2854814325727516 1.2746651694117415 8 0.2176389429250373 1.1105722210235203 9 0.16733932406369 0.9606401436196432 >10 0.8470665816509716 9.99873848683832 >50 0.05863548209587267 2.5761616003453036 >100 0.02882096655385586 3.3113848306408804 >500 0.002152580243196468 0.9246584350573335 >1k 4.783511651547707E-4 0.842047885216788 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3346 0.2542161049625324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3035 0.23058753095077278 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2966 0.22534517851729557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1692 0.128551598803528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.597612222430734E-5 0.0 0.0 0.0 0.0 14-15 1.5195224444861468E-4 0.0 0.0 0.0 0.0 16-17 1.8994030556076835E-4 0.0 0.0 0.0 0.0 18-19 3.4189255000938303E-4 0.0 0.0 3.798806111215367E-5 0.0 20-21 3.798806111215367E-4 0.0 0.0 7.597612222430734E-5 0.0 22-23 3.798806111215367E-4 0.0 0.0 1.8994030556076835E-4 0.0 24-25 5.698209166823051E-4 0.0 0.0 3.4189255000938303E-4 0.0 26-27 6.078089777944587E-4 0.0 0.0 7.217731611309198E-4 0.0 28-29 6.078089777944587E-4 0.0 0.0 0.0013295821389253785 0.0 30-31 6.078089777944587E-4 0.0 0.0 0.0024692239722899885 3.798806111215367E-5 32-33 6.078089777944587E-4 0.0 0.0 0.003950758355663981 7.597612222430734E-5 34-35 6.837851000187661E-4 0.0 0.0 0.006230042022393202 7.597612222430734E-5 36-37 6.837851000187661E-4 0.0 0.0 0.01021878843916934 7.597612222430734E-5 38-39 8.357373444673808E-4 0.0 0.0 0.016106937911553158 1.5195224444861468E-4 40-41 8.357373444673808E-4 0.0 0.0 0.027769272672984333 1.5195224444861468E-4 42-43 9.117134666916881E-4 0.0 0.0 0.0387858103955089 1.5195224444861468E-4 44-45 0.0011016537722524566 0.0 0.0 0.052689440762557146 1.5195224444861468E-4 46-47 0.0012156179555889175 0.0 0.0 0.07992688057997133 1.5195224444861468E-4 48-49 0.0012156179555889175 0.0 0.0 0.09626174685819741 1.8994030556076835E-4 50-51 0.0012156179555889175 0.0 0.0 0.11601553863651731 2.2792836667292202E-4 52-53 0.0012156179555889175 0.0 0.0 0.13493359307036984 2.2792836667292202E-4 54-55 0.0012156179555889175 0.0 0.0 0.16175316421555033 2.2792836667292202E-4 56-57 0.0012156179555889175 0.0 0.0 0.2327908384952777 2.2792836667292202E-4 58-59 0.0012156179555889175 0.0 0.0 0.29870012452486433 2.2792836667292202E-4 60-61 0.0012156179555889175 0.0 0.0 0.34565336805948627 2.2792836667292202E-4 62-63 0.0012156179555889175 0.0 0.0 0.4087895256278857 2.2792836667292202E-4 64-65 0.0012156179555889175 0.0 0.0 0.46656936657947146 2.2792836667292202E-4 66-67 0.0013675702000375321 0.0 0.0 0.567579621076688 2.2792836667292202E-4 68-69 0.0013675702000375321 0.0 0.0 0.6868241449077384 2.2792836667292202E-4 70-71 0.0013675702000375321 0.0 0.0 0.7516697652261848 2.2792836667292202E-4 72-73 0.0013675702000375321 0.0 0.0 0.8207320603280801 2.2792836667292202E-4 74-75 0.0013675702000375321 0.0 0.0 0.9508411696372064 2.2792836667292202E-4 76-77 0.0013675702000375321 0.0 0.0 1.1141898324194672 2.2792836667292202E-4 78-79 0.001405558261149686 0.0 0.0 1.264888470851381 2.2792836667292202E-4 80-81 0.0016714746889347615 0.0 0.0 1.3728125524710095 2.2792836667292202E-4 82-83 0.0016714746889347615 0.0 0.0 1.5501028336814306 2.2792836667292202E-4 84-85 0.0016714746889347615 0.0 0.0 1.8416991907783222 2.2792836667292202E-4 86-87 0.0016714746889347615 0.0 0.0 2.1396775421420555 2.2792836667292202E-4 88-89 0.0016714746889347615 0.0 0.0 2.510327054413339 2.2792836667292202E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5170 0.0 26.426086 1 TCGCGCT 205 0.0 25.486692 60-61 TACATGG 5390 0.0 24.588928 2 GTACCGG 160 1.6654576E-6 24.053322 1 ACATGGG 5415 0.0 23.507746 3 ATTCGCG 230 0.0 22.7164 80-81 GTATCAA 8370 0.0 22.41528 1 TACCTGG 1115 0.0 21.72799 2 GAGTACT 4485 0.0 21.704538 12-13 GTACCTG 1155 0.0 21.241894 1 GTACCGT 135 3.8033168E-4 21.10538 6 GTACACG 160 4.5251807E-5 21.046656 1 CAATTCG 250 0.0 20.899088 78-79 GTACTTT 4655 0.0 20.707874 14-15 AATTCGC 245 0.0 20.356255 78-79 CGATCGT 245 0.0 20.350843 24-25 CGCGCTT 260 0.0 20.09528 62-63 CGCGGAA 240 0.0 19.791933 82-83 CTCGCGC 265 0.0 19.716124 60-61 TATCAAC 9470 0.0 19.563152 2 >>END_MODULE