FastQCFastQC Report
Thu 26 May 2016
SRR936533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391295
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28290.20333574116201095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.1671104977736569No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21010.15101038960105514No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15940.1145695197639609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG52250.026.9161071
TACATGG52450.026.1803112
GTACCTG13000.025.9490741
ACATGGG52050.025.1947943
TACCTGG13250.024.7431892
GTATCAA67150.024.2691731
GAGTACT33750.021.89325512-13
TCAACGC77250.020.789344
TATCAAC77950.020.7855132
TACACTG11700.020.7120175
CAACGCA77800.020.6431145
ATCAACG77850.020.6291143
AACGCAG78950.020.4018736
GGTATCA51500.020.2965551
GTACTTT36700.019.80904214-15
GTATAGA3506.366463E-1119.0049551
CATGGGG25300.018.7802754
CATGGGA27900.018.7331564
AGAGTAC63200.018.60718210-11
AGTACTT38450.018.35201812-13
Produced by FastQC (version 0.11.5)