##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936533_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391295 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40695539048153 31.0 31.0 34.0 30.0 34.0 2 31.48132854642617 31.0 31.0 34.0 28.0 34.0 3 31.52898702288156 33.0 31.0 34.0 28.0 34.0 4 35.14838765322955 37.0 35.0 37.0 32.0 37.0 5 35.11939452093194 37.0 35.0 37.0 32.0 37.0 6 35.22374190951596 37.0 35.0 37.0 32.0 37.0 7 35.08038913386449 37.0 35.0 37.0 32.0 37.0 8 35.03288950222634 37.0 35.0 37.0 32.0 37.0 9 36.53334123963646 39.0 37.0 39.0 32.0 39.0 10-11 36.43224154474788 39.0 37.0 39.0 32.0 39.0 12-13 36.33209635627239 39.0 37.0 39.0 32.0 39.0 14-15 37.42156192611919 40.0 37.0 41.0 32.0 41.0 16-17 37.28941956953773 40.0 37.0 41.0 32.0 41.0 18-19 37.22375951900927 39.5 37.0 41.0 31.0 41.0 20-21 37.126615131945414 39.0 36.5 41.0 31.0 41.0 22-23 36.978149853194324 39.0 36.0 41.0 31.0 41.0 24-25 37.12070229534355 39.0 36.0 41.0 31.0 41.0 26-27 37.10617913526607 39.0 36.0 41.0 31.0 41.0 28-29 37.010174333983805 39.0 36.0 41.0 31.0 41.0 30-31 36.83416097951908 39.0 36.0 41.0 30.0 41.0 32-33 36.68140185941874 39.0 36.0 41.0 30.0 41.0 34-35 36.47047103597727 39.0 35.0 40.5 30.0 41.0 36-37 36.29565440830305 39.0 35.0 40.0 30.0 41.0 38-39 36.10033458037296 39.0 35.0 40.0 29.5 41.0 40-41 35.934232855001994 38.5 35.0 40.0 29.0 41.0 42-43 35.730149249440274 38.0 35.0 40.0 28.0 41.0 44-45 35.502402438016375 38.0 35.0 40.0 27.0 41.0 46-47 35.2866555978423 38.0 34.0 40.0 26.5 41.0 48-49 35.24540086753708 38.0 34.5 40.0 26.0 41.0 50-51 34.36232502812128 37.5 33.5 39.5 25.0 40.5 52-53 34.54047092816405 38.0 33.5 39.5 26.0 40.5 54-55 34.957528058391645 38.0 34.0 40.0 26.0 41.0 56-57 34.87027984719272 38.0 34.0 40.0 26.0 41.0 58-59 34.62044174671799 38.0 34.0 40.0 25.5 41.0 60-61 34.297992158384815 38.0 34.0 40.0 24.5 41.0 62-63 33.96885886889552 37.0 33.0 40.0 23.0 41.0 64-65 33.629420072666115 37.0 33.0 39.5 23.0 41.0 66-67 33.2458626675148 36.0 32.5 39.0 22.0 41.0 68-69 32.83565527080885 36.0 32.0 39.0 21.5 40.5 70-71 32.3930108280415 35.0 31.5 38.5 20.0 40.0 72-73 31.990175340240565 35.0 31.0 38.0 19.5 40.0 74-75 31.543815653761424 35.0 31.0 37.0 18.0 39.0 76-77 31.0941629920326 35.0 31.0 36.5 16.0 39.0 78-79 30.634777311785065 34.5 30.0 36.0 12.0 38.5 80-81 30.14392238885355 34.0 30.0 36.0 7.5 37.5 82-83 29.654400396752663 34.0 29.0 35.0 7.0 37.0 84-85 29.31796563633162 34.0 29.0 35.0 2.0 36.5 86-87 28.987719714366833 34.0 29.0 35.0 2.0 36.0 88-89 28.56959199882124 34.0 29.0 35.0 2.0 36.0 90-91 28.150168368318724 34.0 27.5 35.0 2.0 35.0 92-93 27.768441991094626 33.5 27.0 35.0 2.0 35.0 94-95 27.302434781983692 33.0 26.0 35.0 2.0 35.0 96-97 26.705932961737084 33.0 25.0 35.0 2.0 35.0 98-99 25.966473680995044 33.0 24.5 35.0 2.0 35.0 100-101 23.48666206663576 30.5 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28832685642824174 1101 2 0.4779508613832846 1101 3 0.346460455121516 1101 4 0.5098260300620723 1101 5 0.4848633652259906 1101 6 0.32248067016312376 1101 7 0.260649492989657 1101 8 0.3679909758839912 1101 9 0.5584932818869888 1101 10-11 0.41886890861353265 1101 12-13 0.5514323532654544 1101 14-15 0.722666203212448 1101 16-17 0.8034712180291947 1101 18-19 0.9416225715181454 1101 20-21 0.8041744137701912 1101 22-23 0.5383263978572828 1101 24-25 0.6539864471496202 1101 26-27 0.5403374896705699 1101 28-29 0.5012687781272902 1101 30-31 0.4981067374505983 1101 32-33 0.4555616817198498 1101 34-35 0.4404976071495881 1101 36-37 0.49363258273033495 1101 38-39 0.3214115150429393 1101 40-41 0.3805460461971748 1101 42-43 0.35689927754669526 1101 44-45 0.28022290722133647 1101 46-47 0.46618629906239306 1101 48-49 0.6859102598195079 1101 50-51 0.6626556929454566 1101 52-53 0.5757280034779697 1101 54-55 0.5939856599029838 1101 56-57 0.4048048687877426 1101 58-59 0.5068430234027588 1101 60-61 0.3004926200074678 1101 62-63 0.41559522560356754 1101 64-65 0.45758981964279144 1101 66-67 0.3799807828927939 1101 68-69 0.5091859236128897 1101 70-71 0.6272894337071193 1101 72-73 0.309957239955569 1101 74-75 0.34023272583921127 1101 76-77 0.3132296717837839 1101 78-79 0.164512721906231 1101 80-81 0.3626787338280657 1101 82-83 0.4596982917852763 1101 84-85 0.3986464972044246 1101 86-87 0.42251719262088727 1101 88-89 0.44991947180280434 1101 90-91 0.25816363533089515 1101 92-93 0.3307578564740332 1101 94-95 0.5413449674629796 1101 96-97 0.36775848327135563 1101 98-99 0.39589622252620416 1101 100-101 0.5269607275620913 1102 1 0.2940616796074522 1102 2 0.43860214087436233 1102 3 0.41953648332679094 1102 4 0.4101353948354145 1102 5 0.39664880684244963 1102 6 0.316463490246349 1102 7 0.24269557968896294 1102 8 0.26114438035796894 1102 9 0.47536640608537084 1102 10-11 0.37737912899208226 1102 12-13 0.40326872432078886 1102 14-15 0.30109064092501825 1102 16-17 0.37285088670724065 1102 18-19 0.5123272123405016 1102 20-21 0.4216014949965725 1102 22-23 0.39538169184150007 1102 24-25 0.25621303170873944 1102 26-27 0.2911304853527952 1102 28-29 0.26454626757251987 1102 30-31 0.18324097674179285 1102 32-33 0.152337144393222 1102 34-35 0.033925534861580786 1102 36-37 0.08569607850549943 1102 38-39 0.19861994809750172 1102 40-41 0.2698306372358843 1102 42-43 0.27593070624839555 1102 44-45 0.012837507227651201 1102 46-47 0.07459433319306896 1102 48-49 0.18444294012736862 1102 50-51 0.16355245326726475 1102 52-53 0.20594794943851724 1102 54-55 0.21724372928441227 1102 56-57 0.4840588390274547 1102 58-59 0.4053492218054231 1102 60-61 0.26018692645630637 1102 62-63 0.08570142213554988 1102 64-65 0.05213548251469291 1102 66-67 0.03214592709348807 1102 68-69 -0.2088051695812041 1102 70-71 -0.15505356600877462 1102 72-73 0.01698201836691382 1102 74-75 -0.2251997944080486 1102 76-77 0.09055630883904087 1102 78-79 -0.05755588318133675 1102 80-81 0.014514169540170485 1102 82-83 0.07809725308662152 1102 84-85 0.1067594890049719 1102 86-87 -0.10831668925228044 1102 88-89 -0.18123903263014185 1102 90-91 -0.20583061932331148 1102 92-93 -0.15540841715361253 1102 94-95 0.003881840446876339 1102 96-97 -0.033090165333053534 1102 98-99 0.24076984011813707 1102 100-101 0.2822422015694883 1103 1 0.29260285985940016 1103 2 0.3962131848981265 1103 3 0.26633834074725016 1103 4 0.3451975446309632 1103 5 0.4962593732696732 1103 6 0.34598280404651405 1103 7 0.2180218335779216 1103 8 0.32907569699655426 1103 9 0.5023845626759211 1103 10-11 0.41195423217896376 1103 12-13 0.40529369967703843 1103 14-15 0.3099609779927377 1103 16-17 0.49675210344666 1103 18-19 0.4472473280954219 1103 20-21 0.3154103952682945 1103 22-23 0.46162161506389765 1103 24-25 0.5923532821187152 1103 26-27 0.3633522485894076 1103 28-29 0.12655397249482547 1103 30-31 0.18690217292871836 1103 32-33 0.46143971560546504 1103 34-35 0.33146393215173475 1103 36-37 0.4378836290803676 1103 38-39 0.4452620596626886 1103 40-41 0.46538717866738466 1103 42-43 0.462330296103886 1103 44-45 0.5011901500227864 1103 46-47 0.6052193621940418 1103 48-49 0.41475792749143636 1103 50-51 0.46552446294142413 1103 52-53 0.5273888083879612 1103 54-55 0.6132691528118102 1103 56-57 0.7732115691933359 1103 58-59 0.5004763790535236 1103 60-61 0.5726304099231996 1103 62-63 0.46265156083094894 1103 64-65 0.6849613600983275 1103 66-67 0.4597493092012712 1103 68-69 0.5777864208718455 1103 70-71 0.44835004493792496 1103 72-73 0.4938992232728019 1103 74-75 0.40390858265781304 1103 76-77 0.33579194387833056 1103 78-79 0.3940478565579788 1103 80-81 0.2233873925585108 1103 82-83 0.2105878511121766 1103 84-85 0.1583410465355506 1103 86-87 0.24497685945974013 1103 88-89 0.38269920349947384 1103 90-91 0.4440435871817314 1103 92-93 0.242840037165184 1103 94-95 0.07756185755886591 1103 96-97 0.173210034646349 1103 98-99 0.20998891319606727 1103 100-101 0.5904883244928776 1104 1 0.12493129216615273 1104 2 0.2655613610682721 1104 3 0.27757349949858323 1104 4 0.28880020414460716 1104 5 0.4862599926476321 1104 6 0.20634463988276508 1104 7 0.23579321641066997 1104 8 0.26395822958536286 1104 9 0.4771991066231678 1104 10-11 0.4797606336883504 1104 12-13 0.21780144190145734 1104 14-15 0.36877865694531664 1104 16-17 0.30365179518701524 1104 18-19 0.25906214123385496 1104 20-21 0.2752008549420353 1104 22-23 0.16493897956306114 1104 24-25 0.24752264625578135 1104 26-27 0.04653863847353534 1104 28-29 0.1389337893495295 1104 30-31 0.1830057495981805 1104 32-33 -0.20000323253830743 1104 34-35 -0.199139294330962 1104 36-37 -0.06809158150813488 1104 38-39 -0.08084025442862952 1104 40-41 0.10216220868339576 1104 42-43 -0.03315568927879298 1104 44-45 0.1827709216724287 1104 46-47 0.2183249232551887 1104 48-49 0.2894637229633048 1104 50-51 0.37073164486702126 1104 52-53 0.3910640185000247 1104 54-55 0.5780256538981092 1104 56-57 0.46587563288444755 1104 58-59 0.43591188513295265 1104 60-61 0.3364422405185721 1104 62-63 0.48902306610995083 1104 64-65 0.24359173677541435 1104 66-67 0.5699482605467452 1104 68-69 0.432648895143835 1104 70-71 0.3828541018635221 1104 72-73 0.46847137603999656 1104 74-75 0.3119660942830613 1104 76-77 0.5904506785368149 1104 78-79 0.6064465525291638 1104 80-81 0.4660706926995424 1104 82-83 0.555450944762125 1104 84-85 0.615706701075112 1104 86-87 0.7657894476019855 1104 88-89 0.822579044176404 1104 90-91 0.7610239529005938 1104 92-93 0.5627302881561853 1104 94-95 0.6239142574102168 1104 96-97 0.636879050978866 1104 98-99 1.097203761114958 1104 100-101 1.2623112133669423 1105 1 0.1978232462996914 1105 2 0.3304865288463361 1105 3 0.25585886380139655 1105 4 0.3413587528109332 1105 5 0.48921280640329456 1105 6 0.2693672765631092 1105 7 0.3441169318932751 1105 8 0.3253378167658383 1105 9 0.47805369273499565 1105 10-11 0.3098125433660428 1105 12-13 0.21368079397891648 1105 14-15 0.08213230798832427 1105 16-17 0.17950554641596028 1105 18-19 0.13868188213964316 1105 20-21 0.31779858299195496 1105 22-23 0.2126431852354571 1105 24-25 0.4479778577950597 1105 26-27 0.25037996798110385 1105 28-29 0.07518981386435541 1105 30-31 -0.07014359558647953 1105 32-33 -0.032631089841459016 1105 34-35 -0.09256081801225235 1105 36-37 -0.12543567881940731 1105 38-39 -0.15565527777863508 1105 40-41 -0.30384350458710685 1105 42-43 -0.05399854027691475 1105 44-45 -0.3760432374068259 1105 46-47 -0.21070736066376838 1105 48-49 -0.17004980080346854 1105 50-51 -0.12120505631704503 1105 52-53 -0.17544859557837356 1105 54-55 -0.07052789482165167 1105 56-57 0.05582357626737178 1105 58-59 0.05070544197444349 1105 60-61 -0.19652043821316312 1105 62-63 -0.14482518958595847 1105 64-65 0.02961275845832745 1105 66-67 0.02090907715571433 1105 68-69 0.040602256582673135 1105 70-71 0.25090837726533266 1105 72-73 0.017541116144290214 1105 74-75 0.09696538630237939 1105 76-77 -0.0921741063973549 1105 78-79 -0.0025158483535392406 1105 80-81 -0.02522698913127286 1105 82-83 0.053849639406049477 1105 84-85 0.05413258618454364 1105 86-87 0.19985724409895056 1105 88-89 -0.08374976876708473 1105 90-91 0.013589714954964194 1105 92-93 -0.05148937019638211 1105 94-95 0.0503318551382641 1105 96-97 0.142723398087103 1105 98-99 0.08370604268288062 1105 100-101 0.35078625638010763 1106 1 0.07798835738354626 1106 2 0.11559047597510741 1106 3 0.22441453210727857 1106 4 0.2834576187408828 1106 5 0.2969063435793089 1106 6 0.1408991818082299 1106 7 0.2684795518143943 1106 8 0.24373792679033102 1106 9 0.24029596221323146 1106 10-11 0.3253642827566736 1106 12-13 0.4242503937648152 1106 14-15 0.4177547827217154 1106 16-17 0.5926584916155804 1106 18-19 0.4973749323829182 1106 20-21 0.2936208859979317 1106 22-23 0.34232154943883586 1106 24-25 0.3607577976532994 1106 26-27 0.03610094437415512 1106 28-29 0.4072519182315162 1106 30-31 0.30144276434973705 1106 32-33 0.0060259794182186965 1106 34-35 -0.05768937197071722 1106 36-37 -0.005615843930492304 1106 38-39 0.044438763428509276 1106 40-41 0.02907166132314387 1106 42-43 -0.16214685775705817 1106 44-45 0.09170434558546958 1106 46-47 -0.04031249910506318 1106 48-49 0.06055262047883048 1106 50-51 -0.24508260202738796 1106 52-53 0.22193795095343916 1106 54-55 0.3281057198918944 1106 56-57 -0.01590328792300255 1106 58-59 0.18278445889070838 1106 60-61 0.15238507825149128 1106 62-63 0.18744358241429637 1106 64-65 0.10434726860771093 1106 66-67 0.14150057032936303 1106 68-69 0.25682882526805884 1106 70-71 0.32842620978277637 1106 72-73 0.5856685810089353 1106 74-75 0.21165068279237786 1106 76-77 0.42791428490327377 1106 78-79 0.4346952908832016 1106 80-81 0.5085226531032667 1106 82-83 0.5629480332714429 1106 84-85 0.5066155714167309 1106 86-87 0.5135209111113035 1106 88-89 0.6922801311329287 1106 90-91 0.6956464296086686 1106 92-93 0.7227312586530807 1106 94-95 0.5990719631555557 1106 96-97 0.8320969275316621 1106 98-99 1.1204471617360774 1106 100-101 1.7220089035843316 1107 1 0.1337361577481957 1107 2 0.1958796249450785 1107 3 -0.0040714076268741906 1107 4 0.15733283529999653 1107 5 0.24924366891350758 1107 6 0.3165590724127796 1107 7 0.24156796845521455 1107 8 0.2963657628635943 1107 9 0.24100862136999268 1107 10-11 0.3238708759886748 1107 12-13 0.1860505011932645 1107 14-15 0.19228100055938313 1107 16-17 0.2826445713093406 1107 18-19 0.42563580194813966 1107 20-21 0.3872674766577049 1107 22-23 0.45824214317016043 1107 24-25 0.46846162800712676 1107 26-27 0.28564096903730274 1107 28-29 0.4382264909764615 1107 30-31 0.29489870733700485 1107 32-33 0.40726027179003665 1107 34-35 0.5302317362067086 1107 36-37 0.49230745820823785 1107 38-39 0.40419167799263533 1107 40-41 0.334964717057332 1107 42-43 0.4001862309621984 1107 44-45 0.572022243013997 1107 46-47 0.41866042438621065 1107 48-49 0.3431371069027591 1107 50-51 0.23432555799561072 1107 52-53 0.41802475616750456 1107 54-55 0.5734658699891071 1107 56-57 0.6677180343547775 1107 58-59 0.6852651482484902 1107 60-61 0.5725426651966572 1107 62-63 0.5482832643655655 1107 64-65 0.6162559392941205 1107 66-67 0.5704230327104867 1107 68-69 0.494692814797574 1107 70-71 0.6693435882259706 1107 72-73 0.30693600976193736 1107 74-75 0.5393952149112167 1107 76-77 0.4315766872152551 1107 78-79 0.46206041900297734 1107 80-81 0.5230674817851515 1107 82-83 0.6021594176610314 1107 84-85 0.4501403593968476 1107 86-87 0.5916221510777113 1107 88-89 0.5388250203378782 1107 90-91 0.5967922069896829 1107 92-93 0.5654348242184817 1107 94-95 0.44027190868479593 1107 96-97 0.7282783000435984 1107 98-99 0.8716689712245795 1107 100-101 1.293477949816392 1108 1 0.020997694720552573 1108 2 0.14498869077499066 1108 3 0.04298637201513955 1108 4 0.03945284723826603 1108 5 0.10547859482360167 1108 6 0.03213771580519165 1108 7 -0.03642769182017247 1108 8 0.023508401061235418 1108 9 0.20128481499592255 1108 10-11 -0.022706549376934504 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-2.93286146458847 2316 72-73 -2.6525034483159793 2316 74-75 -2.253232603023733 2316 76-77 -2.1075193522008924 2316 78-79 -2.820199195421658 2316 80-81 -2.4149716448297376 2316 82-83 -2.2913087888444323 2316 84-85 -1.9888609584682087 2316 86-87 -2.9666141311450858 2316 88-89 -3.2195389300624697 2316 90-91 -3.2336243931181414 2316 92-93 -3.9182141644199255 2316 94-95 -4.110062799088613 2316 96-97 -5.385209100367735 2316 98-99 -6.978231689704714 2316 100-101 -7.300540989501911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3487.0 3 1407.0 4 1339.0 5 1413.0 6 1593.0 7 1921.0 8 2338.0 9 2746.0 10 3541.0 11 4287.0 12 5059.0 13 6116.0 14 6728.0 15 7872.0 16 9469.0 17 11436.0 18 9929.0 19 8177.0 20 8527.0 21 9377.0 22 10469.0 23 11715.0 24 13571.0 25 15814.0 26 18304.0 27 21681.0 28 25858.0 29 31274.0 30 38566.0 31 46852.0 32 59155.0 33 76317.0 34 101976.0 35 138756.0 36 196298.0 37 254726.0 38 196264.0 39 26931.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33391668979481 18.227324953516028 14.086980030227163 23.351778326461996 2 15.446830659864611 19.40239490651007 40.700038534758924 24.450735898866387 3 19.777510821437122 23.469488256197568 29.257600167938065 27.495400754427244 4 12.683002601840707 15.816429998403514 37.13181940435251 34.36874799540327 5 13.954234311465012 36.890010169194106 34.340016426053275 14.815739093287613 6 32.84840866564935 35.05998156183617 17.66353083646269 14.428078936051788 7 29.533268515508816 31.71382259284722 20.349153293196142 18.403755598447823 8 26.685760676741953 34.25869531295505 19.687515954620206 19.368028055682792 9 26.2453502728535 16.763969675114136 20.258896232255893 36.73178381977647 10-11 25.192389979396417 26.000999615256 27.531597550583047 21.275012854764533 12-13 26.10865883031419 23.16759973983492 27.963287816429016 22.760453613421873 14-15 22.73195800306388 24.964002472099896 26.08629843307839 26.217741091757834 16-17 22.435914491770838 28.33533366460449 26.706292162464774 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22.398180938669572 26.415130956171605 28.2615055197888 22.925182585370028 44-45 22.510153525692974 26.413685957137584 28.19066526123159 22.885495255937848 46-47 22.679491694758102 26.655190506652097 27.87492183513142 22.790395963458373 48-49 22.751885400700434 26.680526780147098 27.785909763114248 22.781678056038224 50-51 22.506901863089748 26.83277617694645 27.833177962051074 22.82714399791273 52-53 22.841193091357873 26.920135447222375 27.488548010663845 22.750123450755908 54-55 22.465064558955554 26.661127120367965 27.77882403003431 23.094984290642167 56-57 22.59009598352925 26.96661279933749 27.528769179452965 22.914522037680292 58-59 22.87758846563718 26.65335631196548 27.56242598286666 22.906629239530677 60-61 22.650585001827626 26.634664354909376 28.09209218707263 22.62265845619037 62-63 22.50406209534037 26.752253694700784 27.96004644859118 22.783637761367668 64-65 22.38525977596412 26.884197815704074 27.77049439550922 22.960048012822586 66-67 22.325374947437275 26.990932256082 27.48712437868164 23.19656841779909 68-69 22.358838139671974 26.754058998810248 27.88065806641769 23.006444795100087 70-71 22.48949738014039 26.737411322091965 27.69112000750431 23.081971290263336 72-73 22.449966073821066 26.656596629232865 27.76013185208186 23.13330544486421 74-75 22.31304834097878 27.110811778252813 27.539745062325604 23.0363948184428 76-77 22.458434865115475 26.83429294345129 27.532076855066528 23.17519533636671 78-79 22.502059364321315 26.808934119030535 27.578194746141115 23.110811770507027 80-81 22.57742430091156 27.002091101282193 27.47231932532691 22.94816527247934 82-83 22.454126330135164 26.905137390994327 27.579800921786184 23.06093535708433 84-85 22.41591193456197 26.869217749243113 27.481953464866454 23.23291685132847 86-87 22.501127597292328 26.898415613493793 27.529187249435306 23.071269539778577 88-89 22.43988239019185 26.802306336819626 27.25709013840824 23.500721134580278 90-91 22.270053081951776 26.74191102214204 27.619290635366166 23.36874526054002 92-93 22.45895080998487 26.68527139138971 27.713779106275894 23.141998692349524 94-95 22.23766517005453 26.616190151688322 27.75227115564047 23.39387352261667 96-97 22.146222278450438 26.513166028827822 27.83030803159387 23.510303661127868 98-99 22.058655957635487 26.70934894252271 27.519679534254916 23.712315565586888 100-101 22.032396247341808 26.488596959548733 27.69133146023991 23.787675332869554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 57.0 1 40.0 2 45.5 3 75.0 4 70.0 5 78.0 6 144.5 7 266.5 8 430.0 9 536.5 10 559.0 11 569.5 12 623.5 13 737.0 14 816.0 15 877.0 16 970.0 17 1069.5 18 1155.5 19 1284.0 20 1426.5 21 1680.5 22 2041.5 23 2479.5 24 3105.5 25 3892.5 26 5046.5 27 6734.5 28 8499.0 29 10636.5 30 13487.0 31 16811.0 32 20407.5 33 23439.5 34 26462.0 35 29554.0 36 32967.0 37 36484.0 38 38715.0 39 40153.0 40 42016.5 41 44911.5 42 47222.5 43 49696.5 44 52063.5 45 53696.0 46 54737.5 47 56380.5 48 60855.5 49 60417.5 50 55280.5 51 51938.0 52 49579.5 53 48482.0 54 47085.5 55 43719.5 56 39337.0 57 34924.5 58 31592.5 59 27959.5 60 23154.5 61 18715.5 62 15254.0 63 12029.5 64 8930.0 65 6744.0 66 5074.5 67 3943.0 68 2982.0 69 2059.5 70 1515.5 71 1177.0 72 904.5 73 706.0 74 547.0 75 425.0 76 304.0 77 170.5 78 103.5 79 78.5 80 49.5 81 34.0 82 27.0 83 16.0 84 9.5 85 10.5 86 9.5 87 5.5 88 4.5 89 4.0 90 3.0 91 2.0 92 1.5 93 1.0 94 1.5 95 2.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03543461307630661 2 0.024653290639296485 3 0.02199389777150065 4 0.05325973283883 5 0.059009771471902076 6 0.050384713522293983 7 0.052540978009696 8 0.04542530520126932 9 0.04470655037213531 10-11 0.05462536701418462 12-13 0.045964371323119826 14-15 0.041292464933748774 16-17 0.049737834176073366 18-19 0.0527206667169795 20-21 0.012254769836734842 22-23 0.020628263596146036 24-25 0.005354723477048361 26-27 0.011571952749057533 28-29 0.006864108618229779 30-31 0.00948756374456891 32-33 0.0037734628529535433 34-35 0.009235999554372005 36-37 0.010134443090789516 38-39 0.01135632630031733 40-41 0.0023000154532288263 42-43 0.002012513521575223 44-45 0.0021203267459453242 46-47 3.593774145670041E-4 48-49 8.984435364175103E-4 50-51 2.875019316536033E-4 52-53 0.003737525111496843 54-55 0.00618129153055247 56-57 0.016028232689688383 58-59 0.010601633729726622 60-61 0.01045788276389982 62-63 0.0057141008916153655 64-65 0.0 66-67 0.0071875482913400825 68-69 0.017968870728350204 70-71 0.006792233135316379 72-73 0.002803143833622632 74-75 0.0038094005944102434 76-77 0.007546925705907087 78-79 0.006396917979292673 80-81 0.011643828231970932 82-83 0.005965665081812269 84-85 0.017142302674846097 86-87 0.004492217682087551 88-89 0.00607347830618237 90-91 0.0035937741456700412 92-93 0.018004808469806906 94-95 0.010996948885750325 96-97 0.009990692124962714 98-99 0.022964216790831563 100-101 0.039351826895086955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.5583401383991 #Duplication Level Percentage of deduplicated Percentage of total 1 83.19508902652534 54.54131944245343 2 10.275559326246947 13.472972268447931 3 2.7982546641244435 5.503467931935961 4 1.1908088702574195 3.1226981182463476 5 0.6502324368972311 2.1314079633564407 6 0.3977915554810715 1.5647132459046569 7 0.2733818634002545 1.2545722831924275 8 0.19522307574765585 1.023880064218342 9 0.13231651524318117 0.7807006001016127 >10 0.8042287551195605 9.590784228144278 >50 0.05907752889219495 2.641783831902042 >100 0.026277270652875176 3.120285863161673 >500 9.89500169144311E-4 0.37617385643827084 >1k 7.696112426677973E-4 0.8752403024966321 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2829 0.20333574116201095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 0.1671104977736569 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2101 0.15101038960105514 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1594 0.1145695197639609 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 7.187548291340082E-5 0.0 9 0.0 0.0 0.0 7.187548291340082E-5 0.0 10-11 0.0 0.0 0.0 1.4375096582680164E-4 0.0 12-13 7.187548291340082E-5 0.0 0.0 1.4375096582680164E-4 3.593774145670041E-5 14-15 7.187548291340082E-5 0.0 0.0 1.4375096582680164E-4 7.187548291340082E-5 16-17 7.187548291340082E-5 0.0 0.0 1.7968870728350206E-4 7.187548291340082E-5 18-19 1.0781322437010123E-4 0.0 0.0 2.1562644874020248E-4 7.187548291340082E-5 20-21 1.4375096582680164E-4 0.0 0.0 2.1562644874020248E-4 7.187548291340082E-5 22-23 1.4375096582680164E-4 0.0 0.0 3.593774145670041E-4 7.187548291340082E-5 24-25 1.7968870728350206E-4 0.0 0.0 6.828170876773077E-4 7.187548291340082E-5 26-27 2.1562644874020248E-4 0.0 0.0 0.0010781322437010124 7.187548291340082E-5 28-29 2.1562644874020248E-4 0.0 0.0 0.0018328248142917211 7.187548291340082E-5 30-31 3.593774145670041E-4 0.0 0.0 0.003162521248189636 7.187548291340082E-5 32-33 3.593774145670041E-4 0.0 0.0 0.004995346062481357 1.4375096582680164E-4 34-35 3.593774145670041E-4 0.0 0.0 0.0069719218425998795 1.4375096582680164E-4 36-37 3.953151560237045E-4 0.0 0.0 0.010314131798073018 1.4375096582680164E-4 38-39 4.3125289748040496E-4 0.0 0.0 0.015920419465318282 1.4375096582680164E-4 40-41 4.3125289748040496E-4 0.0 0.0 0.029361134770124236 1.4375096582680164E-4 42-43 5.031283803938058E-4 0.0 0.0 0.04183153105559928 1.4375096582680164E-4 44-45 5.750038633072066E-4 0.0 0.0 0.05570349925788564 1.4375096582680164E-4 46-47 6.828170876773079E-4 0.0 0.0 0.08136304665796973 1.4375096582680164E-4 48-49 7.90630312047409E-4 0.0 0.0 0.09731940386474472 1.4375096582680164E-4 50-51 7.90630312047409E-4 0.0 0.0 0.11557577652474853 1.4375096582680164E-4 52-53 8.625057949608099E-4 0.0 0.0 0.13598841367215436 1.4375096582680164E-4 54-55 8.625057949608099E-4 0.0 0.0 0.1652417352179085 1.4375096582680164E-4 56-57 8.625057949608099E-4 0.0 0.0 0.2360031481461516 1.4375096582680164E-4 58-59 8.984435364175103E-4 0.0 0.0 0.2988223202124639 1.4375096582680164E-4 60-61 9.343812778742107E-4 0.0 0.0 0.3436007460675127 2.1562644874020248E-4 62-63 9.343812778742107E-4 0.0 0.0 0.4013167588469735 2.1562644874020248E-4 64-65 9.343812778742107E-4 0.0 0.0 0.46248279480627763 2.1562644874020248E-4 66-67 9.343812778742107E-4 0.0 0.0 0.5664147430990552 2.1562644874020248E-4 68-69 9.343812778742107E-4 0.0 0.0 0.6900765114515613 2.1562644874020248E-4 70-71 9.343812778742107E-4 0.0 0.0 0.7562378934733468 2.1562644874020248E-4 72-73 9.343812778742107E-4 0.0 0.0 0.8278618121965506 2.515641901969029E-4 74-75 9.343812778742107E-4 0.0 0.0 0.9618377123471298 2.875019316536033E-4 76-77 0.0010062567607876116 0.0 0.0 1.1190293934787374 2.875019316536033E-4 78-79 0.0010781322437010124 0.0 0.0 1.2661944447439257 2.875019316536033E-4 80-81 0.001221883209527814 0.0 0.0 1.3768108129476495 3.2343967311030373E-4 82-83 0.001221883209527814 0.0 0.0 1.5429509917019755 3.593774145670041E-4 84-85 0.001221883209527814 3.593774145670041E-5 0.0 1.8337951333110518 3.593774145670041E-4 86-87 0.001221883209527814 7.187548291340082E-5 0.0 2.1278377339097747 3.593774145670041E-4 88-89 0.001221883209527814 7.187548291340082E-5 0.0 2.4853463859210305 3.593774145670041E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5225 0.0 26.916107 1 TACATGG 5245 0.0 26.180311 2 GTACCTG 1300 0.0 25.949074 1 ACATGGG 5205 0.0 25.194794 3 TACCTGG 1325 0.0 24.743189 2 GTATCAA 6715 0.0 24.269173 1 GAGTACT 3375 0.0 21.893255 12-13 TCAACGC 7725 0.0 20.78934 4 TATCAAC 7795 0.0 20.785513 2 TACACTG 1170 0.0 20.712017 5 CAACGCA 7780 0.0 20.643114 5 ATCAACG 7785 0.0 20.629114 3 AACGCAG 7895 0.0 20.401873 6 GGTATCA 5150 0.0 20.296555 1 GTACTTT 3670 0.0 19.809042 14-15 GTATAGA 350 6.366463E-11 19.004955 1 CATGGGG 2530 0.0 18.780275 4 CATGGGA 2790 0.0 18.733156 4 AGAGTAC 6320 0.0 18.607182 10-11 AGTACTT 3845 0.0 18.352018 12-13 >>END_MODULE