FastQCFastQC Report
Thu 26 May 2016
SRR936533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391295
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.24811416701705966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33520.24092661872571955No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30720.22080148350996734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.12721960475671945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG52300.026.5324441
GTAAGGT4850.025.4648044
TACATGG54200.024.9760132
ACATGGG51950.024.7794953
GTATAGC4850.024.4959551
TAGCGCG701.7799332E-523.75125528-29
GAGTACT47400.020.74364912-13
CATGGGG26050.020.4229774
GGGCGTA3052.0918378E-1020.2480629
GTATCAA91100.020.135621
GTGTAGG7450.019.7743171
AGTACTT49300.019.558812-13
GGTAAGG5600.019.5096073
TAAGGTG6100.019.467895
GTACTTT49950.019.35113514-15
TACCTGG11900.018.7598082
GGTATCA72650.018.7079621
GTACCTG12950.018.3483221
ATTCGCG1708.931238E-1018.1610980-81
AGAGTAC81150.017.85336310-11
Produced by FastQC (version 0.11.5)