##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936533_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391295 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01656083001808 31.0 31.0 34.0 30.0 34.0 2 32.170032236154086 33.0 31.0 34.0 30.0 34.0 3 32.27936778325229 34.0 31.0 34.0 30.0 34.0 4 35.99346004980971 37.0 35.0 37.0 35.0 37.0 5 35.790638937105356 37.0 35.0 37.0 35.0 37.0 6 35.89589267552891 37.0 35.0 37.0 35.0 37.0 7 35.84891414114188 37.0 35.0 37.0 35.0 37.0 8 35.81289661789915 37.0 35.0 37.0 35.0 37.0 9 37.48720652341883 39.0 37.0 39.0 35.0 39.0 10-11 37.35266568197255 39.0 37.0 39.0 34.0 39.0 12-13 37.265593565706766 39.0 37.0 39.0 34.0 39.0 14-15 38.42254087019647 40.0 38.0 41.0 34.0 41.0 16-17 38.3487269055089 40.0 38.0 41.0 33.5 41.0 18-19 38.47411404482874 40.0 38.0 41.0 34.0 41.0 20-21 38.49338062740109 40.0 38.0 41.0 34.0 41.0 22-23 38.40648999672966 40.0 38.0 41.0 34.0 41.0 24-25 38.286047890634265 40.0 38.0 41.0 34.0 41.0 26-27 38.09306042212471 40.0 37.5 41.0 33.0 41.0 28-29 38.026109128545706 40.0 37.5 41.0 33.0 41.0 30-31 38.03160400921443 40.0 37.0 41.0 33.0 41.0 32-33 37.92595890878642 40.0 37.0 41.0 33.0 41.0 34-35 37.77687801652417 40.0 37.0 41.0 33.0 41.0 36-37 37.54756180393087 40.0 37.0 41.0 32.5 41.0 38-39 37.401400493784564 40.0 37.0 41.0 32.0 41.0 40-41 37.19831595743534 39.0 36.0 41.0 31.5 41.0 42-43 36.977807007140825 39.0 36.0 41.0 31.0 41.0 44-45 36.75809479657441 39.0 36.0 41.0 31.0 41.0 46-47 36.442474457250256 39.0 35.5 40.0 30.0 41.0 48-49 36.12524231022177 39.0 35.0 40.0 30.0 41.0 50-51 35.827232542343644 39.0 35.0 40.0 29.0 41.0 52-53 35.56796797228482 38.0 35.0 40.0 28.0 41.0 54-55 35.30834761858556 38.0 34.0 40.0 28.0 41.0 56-57 35.00024329850966 38.0 34.0 40.0 27.0 41.0 58-59 34.66185711872752 38.0 34.0 40.0 26.0 41.0 60-61 34.30603358741317 37.0 33.5 40.0 26.0 41.0 62-63 33.91088985441621 37.0 33.0 39.5 25.5 41.0 64-65 33.50067778580387 36.0 32.5 39.0 24.5 40.0 66-67 33.04421276580452 36.0 32.0 39.0 23.0 40.0 68-69 32.75667274014498 35.0 32.0 38.5 23.0 40.0 70-71 32.323324312960224 35.0 31.0 38.0 22.0 40.0 72-73 31.866581494219417 35.0 31.0 37.0 21.5 39.0 74-75 31.332549531192164 34.0 30.5 36.5 20.0 39.0 76-77 29.9282380803496 33.0 28.5 35.0 18.5 37.5 78-79 30.70584527364793 34.0 30.0 36.0 19.5 37.5 80-81 30.512380911309247 34.0 30.0 35.0 18.0 37.0 82-83 30.166532618891033 34.0 30.0 35.0 17.0 37.0 84-85 29.74120765186391 34.0 29.5 35.0 12.0 36.0 86-87 29.35810378100978 34.0 29.0 35.0 7.0 36.0 88-89 28.950405916789755 34.0 29.0 35.0 4.5 35.5 90-91 28.473062147136304 33.0 29.0 35.0 2.0 35.0 92-93 27.983456060720407 33.0 27.5 35.0 2.0 35.0 94-95 27.393355470982073 33.0 26.5 34.5 2.0 35.0 96-97 26.61083702593627 32.0 25.0 34.0 2.0 35.0 98-99 25.630022029835516 32.0 24.0 34.0 2.0 35.0 100-101 22.34961564585512 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23477875752391242 1101 2 0.28414592010874884 1101 3 0.2220905986996513 1101 4 0.17516435198416502 1101 5 0.15546233150924849 1101 6 0.16367892349256152 1101 7 0.18536597236025898 1101 8 0.1501837184630901 1101 9 0.28789233753212784 1101 10-11 0.21549645767137093 1101 12-13 0.2606454737192436 1101 14-15 0.41120905216203596 1101 16-17 0.4014099959882458 1101 18-19 0.2131042223699282 1101 20-21 0.13244941165936552 1101 22-23 0.17631191622570697 1101 24-25 0.1531251089269432 1101 26-27 0.12073670134950731 1101 28-29 0.14337434778546765 1101 30-31 0.2946744686983678 1101 32-33 0.3137107719473988 1101 34-35 0.26069554135219875 1101 36-37 0.15141753090689747 1101 38-39 0.22085648696850768 1101 40-41 0.23981126614839354 1101 42-43 0.3034313229642649 1101 44-45 0.2946576455291492 1101 46-47 0.342461548563989 1101 48-49 0.2779348280787701 1101 50-51 0.48690259287363347 1101 52-53 0.32138112927566453 1101 54-55 0.24108108350660729 1101 56-57 0.1147734819049191 1101 58-59 0.22894808804393563 1101 60-61 0.14703025230491562 1101 62-63 0.15736191750788464 1101 64-65 0.2935267910283912 1101 66-67 0.07708868954290438 1101 68-69 0.22135552970559047 1101 70-71 0.21754616708143004 1101 72-73 0.22409971399582673 1101 74-75 0.14927387534447334 1101 76-77 0.07838141902830031 1101 78-79 -0.04717482054997291 1101 80-81 0.08744516005709002 1101 82-83 0.2131629905098258 1101 84-85 0.12365841486310103 1101 86-87 0.11520005561527569 1101 88-89 0.03856220059215687 1101 90-91 0.06663463472326114 1101 92-93 0.12050991256359822 1101 94-95 0.2790514740286909 1101 96-97 0.22450857422966308 1101 98-99 0.34258454192428545 1101 100-101 0.7916579679075078 1102 1 0.17774854243004512 1102 2 0.20855206484610278 1102 3 0.20513224134943187 1102 4 0.14616929644230936 1102 5 0.020862348579264278 1102 6 0.096727639675521 1102 7 0.09923839855875372 1102 8 0.12267585865700426 1102 9 0.2419167102383426 1102 10-11 0.24425446216335445 1102 12-13 0.12268030122441331 1102 14-15 0.347567421905552 1102 16-17 0.2649349421767866 1102 18-19 0.21166174773234303 1102 20-21 0.0818482197982604 1102 22-23 0.115272878506822 1102 24-25 0.13247886859751645 1102 26-27 -0.07218368574229572 1102 28-29 0.007522758695778009 1102 30-31 0.12913742384515814 1102 32-33 0.10264268012942779 1102 34-35 0.12475246400282458 1102 36-37 0.23478116546362315 1102 38-39 0.14187581232881286 1102 40-41 0.16338641688479782 1102 42-43 0.08452943929990653 1102 44-45 0.18279117980539183 1102 46-47 0.25059242880381305 1102 48-49 -0.0215933497206251 1102 50-51 -0.13234899224084984 1102 52-53 -0.180523755671274 1102 54-55 -0.12578040736035945 1102 56-57 -0.0997735021392927 1102 58-59 -0.27059184000479064 1102 60-61 -0.19128251711777722 1102 62-63 -0.3136275353280169 1102 64-65 -0.06872462689348424 1102 66-67 -0.1455361524717773 1102 68-69 -0.12797645985130401 1102 70-71 -0.24287010742310144 1102 72-73 -0.1644824927360773 1102 74-75 -0.33240976585318904 1102 76-77 -0.5718162891469483 1102 78-79 -0.49642163541398077 1102 80-81 -0.06610312294503373 1102 82-83 -0.2914014844527877 1102 84-85 -0.2741901203309389 1102 86-87 -0.24170441427310152 1102 88-89 -0.180564457742161 1102 90-91 -0.046538388574973055 1102 92-93 -0.017340095796029686 1102 94-95 0.10308798281331732 1102 96-97 0.11985652775713618 1102 98-99 0.20342066862120234 1102 100-101 0.7485725511945631 1103 1 0.07417799904515476 1103 2 0.09383552744361623 1103 3 0.08896027943492157 1103 4 0.09775209889276937 1103 5 -0.18234252788507632 1103 6 0.037713910472760404 1103 7 0.008010669892435374 1103 8 0.037199093954782825 1103 9 0.0760860261322378 1103 10-11 0.04453309786697446 1103 12-13 0.1118984462711623 1103 14-15 -0.011028636678112491 1103 16-17 0.25862631069456654 1103 18-19 0.1981538011824142 1103 20-21 0.16876484751401932 1103 22-23 0.02600080579444608 1103 24-25 0.05403278890680241 1103 26-27 0.18297084842566136 1103 28-29 0.368642892332268 1103 30-31 0.17736618579497332 1103 32-33 0.2174930464188094 1103 34-35 0.15686743574879358 1103 36-37 0.1922498925104179 1103 38-39 0.09436344518157114 1103 40-41 0.18945673674998886 1103 42-43 0.2988738022461135 1103 44-45 0.23356721758757004 1103 46-47 0.20569297871887215 1103 48-49 0.24893707668706355 1103 50-51 0.38359928020647516 1103 52-53 0.20234314768106998 1103 54-55 0.07943897581579762 1103 56-57 0.04240794832817585 1103 58-59 0.28278466564092497 1103 60-61 0.050019454043088274 1103 62-63 0.05944920492850514 1103 64-65 -0.01295051873710662 1103 66-67 0.3117158453416877 1103 68-69 0.42962654878051154 1103 70-71 0.32402735984399555 1103 72-73 0.18106963488858696 1103 74-75 -0.03333700481329416 1103 76-77 -0.05234291289028903 1103 78-79 0.08090260170477492 1103 80-81 0.10906021620169426 1103 82-83 0.14965863272812285 1103 84-85 0.16472816878698637 1103 86-87 0.12826398739690248 1103 88-89 0.05298194551454927 1103 90-91 -0.05504996236873083 1103 92-93 0.2884727746383682 1103 94-95 0.39014754996599166 1103 96-97 0.15844055588779682 1103 98-99 0.3551854557229248 1103 100-101 0.670322546384643 1104 1 0.13140494865637464 1104 2 0.16474596539147512 1104 3 0.09769231747425522 1104 4 0.07078890900101698 1104 5 0.02868609214084472 1104 6 0.08688656445964682 1104 7 0.08834220385340785 1104 8 0.07899817251097119 1104 9 0.05692461623743128 1104 10-11 0.15115007930469204 1104 12-13 0.04044962594794299 1104 14-15 0.09952764810234527 1104 16-17 0.13396845745856467 1104 18-19 0.13114157323227005 1104 20-21 0.043617447472094284 1104 22-23 0.1778746623274614 1104 24-25 0.10300771839300182 1104 26-27 0.06444215693306177 1104 28-29 0.0010810855591287805 1104 30-31 -0.0835771506511307 1104 32-33 0.03421732317593751 1104 34-35 -0.035810921279704644 1104 36-37 -0.017503940486236047 1104 38-39 0.05632946991857324 1104 40-41 -0.028743267538018813 1104 42-43 -0.08180958603715993 1104 44-45 -0.009475202655600867 1104 46-47 -0.14832440197940144 1104 48-49 -0.13391860110299092 1104 50-51 -0.20078352157352697 1104 52-53 -0.2370108172907237 1104 54-55 -0.3007885145600184 1104 56-57 -0.0024373977611631403 1104 58-59 -0.1371937871529454 1104 60-61 0.012669772308065319 1104 62-63 0.014809325564272058 1104 64-65 0.1164941136382538 1104 66-67 -0.2022380252940792 1104 68-69 0.12979491238396434 1104 70-71 -0.0010364837297203167 1104 72-73 -0.09295716750126104 1104 74-75 0.005083783893063298 1104 76-77 0.018787880675805724 1104 78-79 0.0015847035876852544 1104 80-81 0.2046871100688996 1104 82-83 0.12779429317560087 1104 84-85 0.0028323005143882085 1104 86-87 -0.03331742745292132 1104 88-89 -0.16974785561748362 1104 90-91 -0.04562529302127061 1104 92-93 -0.21801104053925258 1104 94-95 -0.14569061133908434 1104 96-97 0.2178293727806171 1104 98-99 0.5670868626637677 1104 100-101 1.000423004724329 1105 1 0.021673954598071532 1105 2 0.048196983814996486 1105 3 0.08954052107347366 1105 4 0.04544253279343735 1105 5 -0.08462721736159295 1105 6 -0.05264507292601195 1105 7 -0.052295820319457675 1105 8 -0.02466322303916968 1105 9 -0.17130097045146186 1105 10-11 -0.10347817740642284 1105 12-13 -0.21966047172667658 1105 14-15 -0.1059752653049415 1105 16-17 -0.0036821888075735387 1105 18-19 -0.11705791835745316 1105 20-21 -0.11453725432110673 1105 22-23 0.012160430045675241 1105 24-25 -0.04497026207207 1105 26-27 0.03188640011507715 1105 28-29 0.08731096154229334 1105 30-31 -0.0780812836553153 1105 32-33 -0.1830215388185934 1105 34-35 -9.485500520440837E-4 1105 36-37 -0.14217750128173634 1105 38-39 0.06815708864559866 1105 40-41 -0.02417629122998477 1105 42-43 -0.037404169584227986 1105 44-45 0.02623190306594836 1105 46-47 0.02667773532076012 1105 48-49 -0.1382942222558512 1105 50-51 0.004007403717153579 1105 52-53 -0.09642221818411656 1105 54-55 0.007270743422793657 1105 56-57 -0.040087275935057676 1105 58-59 -0.11639635378328705 1105 60-61 0.02986635583376085 1105 62-63 -0.046080686297749196 1105 64-65 -0.26770996913938205 1105 66-67 -0.07392721376120193 1105 68-69 0.028508090709600253 1105 70-71 -0.21381272977571797 1105 72-73 -0.09506758443509256 1105 74-75 -0.22267476931502372 1105 76-77 -0.3069540296430837 1105 78-79 -0.1656961029225279 1105 80-81 0.032169417230992536 1105 82-83 -0.060998940399247914 1105 84-85 -0.2402749064617602 1105 86-87 -0.15570457800434667 1105 88-89 -0.08435553413547936 1105 90-91 0.21109684994273792 1105 92-93 0.25271095281374656 1105 94-95 0.22764763778462438 1105 96-97 0.2535035841168565 1105 98-99 0.594531299538609 1105 100-101 0.5423731976156709 1106 1 0.01619726232096852 1106 2 0.07220908650948132 1106 3 0.03230994169786072 1106 4 -0.031394709011408395 1106 5 -0.1465516795982822 1106 6 0.03982717004970482 1106 7 -0.03436257099131268 1106 8 -0.11721203711636008 1106 9 0.08189550133059953 1106 10-11 0.009229465950660654 1106 12-13 -0.04764237715765063 1106 14-15 -0.019642091804492168 1106 16-17 -0.03532259456007125 1106 18-19 -0.09841248344474707 1106 20-21 -0.03847445858380638 1106 22-23 -0.018767418417887427 1106 24-25 -0.08067822881196207 1106 26-27 -0.06493478406073194 1106 28-29 -0.0676325208073294 1106 30-31 -0.13778425468839828 1106 32-33 -0.14345639576816183 1106 34-35 -0.05244038556542563 1106 36-37 0.018829036998241122 1106 38-39 0.08574037830366876 1106 40-41 0.14020811056757054 1106 42-43 0.06755473849700167 1106 44-45 0.21511961489098042 1106 46-47 0.06751410733736662 1106 48-49 0.055054127934276664 1106 50-51 0.12318047299169166 1106 52-53 -0.09243104244600886 1106 54-55 -0.07617428707555263 1106 56-57 0.08302455876057024 1106 58-59 -0.11305510314673484 1106 60-61 -0.15612875253665237 1106 62-63 -0.164650107665544 1106 64-65 -0.025261515243734323 1106 66-67 -0.09617241970401125 1106 68-69 -0.1259237405663427 1106 70-71 -0.22118399925767562 1106 72-73 -0.19896211703542122 1106 74-75 -0.29350656936780695 1106 76-77 -0.44147997646705406 1106 78-79 -0.30801836318250153 1106 80-81 -0.25964955530377054 1106 82-83 -0.02489640946414795 1106 84-85 -0.19266459389555024 1106 86-87 -0.12407829701813711 1106 88-89 -0.09682714521375502 1106 90-91 -0.17502649643741464 1106 92-93 -0.21569640544608149 1106 94-95 0.010132370027374549 1106 96-97 -0.0502995092069618 1106 98-99 0.40582181996190414 1106 100-101 0.6586770808112874 1107 1 0.0016293592184055683 1107 2 0.09513219951362117 1107 3 -0.03503861823132581 1107 4 0.03273780625175249 1107 5 0.03727277323532974 1107 6 0.07600180937003387 1107 7 0.02765768910658295 1107 8 -0.0783373123071982 1107 9 -0.0446786659097711 1107 10-11 -0.023294875671815873 1107 12-13 -0.09545144200055233 1107 14-15 0.11014358087161469 1107 16-17 0.11376464188086999 1107 18-19 -0.06404824047488233 1107 20-21 -0.09399681051033326 1107 22-23 -0.055869573795739313 1107 24-25 -0.11152948580048871 1107 26-27 -0.09649643059086799 1107 28-29 -0.17018999881097585 1107 30-31 -0.23196231356221375 1107 32-33 -0.11538314772192848 1107 34-35 -0.17088479134235257 1107 36-37 -0.040578139524861 1107 38-39 -0.026989136528264623 1107 40-41 -0.2608517997069697 1107 42-43 -0.38795096958509845 1107 44-45 -0.23917147081725432 1107 46-47 -0.07302424537596153 1107 48-49 -0.1661497515945527 1107 50-51 -0.12369880617853823 1107 52-53 -0.14021778940661278 1107 54-55 -0.008149760101169079 1107 56-57 -0.17542710978482745 1107 58-59 -0.09282451024760974 1107 60-61 -0.02427205603715521 1107 62-63 -0.3436936161879487 1107 64-65 -0.45062446453297156 1107 66-67 -0.1968343907891139 1107 68-69 -0.1345070467903895 1107 70-71 -0.19875377101004688 1107 72-73 -0.123080560684663 1107 74-75 0.01172413701208086 1107 76-77 -0.16589271354726876 1107 78-79 -0.25906011653585637 1107 80-81 -0.06810841096381282 1107 82-83 -0.02935393361663685 1107 84-85 -0.01150126482405156 1107 86-87 -0.04548682017956196 1107 88-89 -0.0848227724219015 1107 90-91 -0.17717014794557784 1107 92-93 -0.05932294783870873 1107 94-95 0.225383427289767 1107 96-97 0.2175411789403512 1107 98-99 0.5915327012534668 1107 100-101 0.9275065646251477 1108 1 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-0.3301216626497201 2316 78-79 -0.4810430042577458 2316 80-81 -0.9470757339006539 2316 82-83 -0.5271886913091741 2316 84-85 -0.5783298774715604 2316 86-87 -0.8736277319025731 2316 88-89 -0.7536498974793098 2316 90-91 -0.9046435160891839 2316 92-93 -0.7122751652465098 2316 94-95 -0.6553510328847523 2316 96-97 -0.6891524773364779 2316 98-99 -0.8376857149123431 2316 100-101 -1.3157088014817582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 14.0 5 39.0 6 89.0 7 190.0 8 398.0 9 792.0 10 1290.0 11 2070.0 12 3096.0 13 4178.0 14 5587.0 15 7405.0 16 9374.0 17 12084.0 18 10563.0 19 7467.0 20 7092.0 21 7367.0 22 8148.0 23 8919.0 24 10657.0 25 12847.0 26 15909.0 27 19880.0 28 25207.0 29 31349.0 30 39114.0 31 48943.0 32 62175.0 33 80762.0 34 108086.0 35 150115.0 36 218300.0 37 288401.0 38 174546.0 39 8835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74285925882253 19.1658391975191 14.492657754206792 22.598643789451582 2 16.063176522933585 20.461496550132868 39.3972741772827 24.078052749650844 3 20.292775001275842 23.81689990001718 29.496082269114076 26.3942428295929 4 13.441046861062775 16.68201221462492 36.50673515021746 33.37020577409485 5 14.07351503651314 37.40936403887068 33.89554942211488 14.621571502501293 6 33.1014144416948 35.79351839406709 16.88726513719991 14.217802027038196 7 29.840041112776227 32.191878789185616 19.913821295986832 18.054258802051326 8 26.935768474694438 35.04612609115967 18.971102462094667 19.047002972051217 9 26.51404831556779 16.906854887980046 19.816786101906892 36.76231069454527 10-11 25.46638338061556 26.510090268678514 26.934516215514943 21.089010135190982 12-13 26.466056842125692 23.513943510069364 27.723321314377575 22.296678333427373 14-15 22.949227769741512 25.41921456231301 25.767207841798367 25.864349826147116 16-17 22.587299619229366 28.64196599119861 26.49222016772596 22.278514221846063 18-19 22.362259621431832 27.175688836659372 28.7953669063714 21.666684635537393 20-21 23.089387944325253 26.121455191027067 28.99500106016337 21.794155804484312 22-23 22.783204272641445 26.096470815704066 28.858550511924175 22.26177439973032 24-25 22.908204740810806 26.33235126476966 28.936066833371548 21.823377161047983 26-27 22.651819330576053 26.501697064315934 28.903787019506538 21.942696585601475 28-29 22.30851367024016 26.827835083781054 28.98476188627858 21.878889359700203 30-31 22.449060269471957 26.428391480773232 28.86209175530529 22.26045649444952 32-33 22.38583155111251 26.6592442904545 28.797513959182442 22.157410199250553 34-35 22.98153478114604 26.491205874826402 28.558263774874998 21.96899556915256 36-37 23.043679380472863 26.260499142600725 28.613881350029168 22.08194012689725 38-39 22.594688896540372 26.558787947620726 28.785633007250777 22.060890148588122 40-41 22.771717157196587 26.5185882657361 28.6073310847842 22.102363492283118 42-43 22.705020603225645 26.597028506699193 28.302248206157323 22.39570268391784 44-45 22.855646806326828 26.61584782955576 28.164455516566733 22.364049847550678 46-47 23.16515302834878 26.982317546716384 27.674191193884546 22.178338231050294 48-49 23.231819010027806 27.01617178119597 27.529870842097736 22.22213836667849 50-51 22.993776248254306 27.144621187781347 27.620728493052816 22.240874070911527 52-53 23.286107659011993 27.243015878378134 27.233707789253366 22.23716867335651 54-55 22.792225831690025 27.076039974167088 27.44010146282278 22.691632731320105 56-57 23.013942616623325 27.31248481607505 27.305225282726482 22.368347284575144 58-59 23.314730606666433 26.97534327115933 27.242398835320046 22.46752728685419 60-61 23.017543052591062 27.0731857210269 27.78237601209573 22.126895214286304 62-63 22.8486816450999 27.099008246344493 27.79009108895234 22.262219019603265 64-65 22.795391690162862 27.200385283742428 27.531901817338845 22.47232120875586 66-67 22.796353239001714 27.36062653907028 27.263232949652906 22.5797872722751 68-69 22.721368954006895 27.142207163044507 27.4936531987016 22.642770684246997 70-71 22.92362361945067 27.217971183660676 27.344579937813286 22.513825259075364 72-73 22.909105415063074 27.051991462516618 27.45349324882581 22.5854098735945 74-75 22.845307352178512 27.566940660078416 27.0273038609818 22.560448126761276 76-77 22.94327021078704 27.278902176585003 27.12127550382315 22.65655210880481 78-79 22.958045177472144 27.258541171532148 27.09102951921173 22.692384131783978 80-81 23.065812266843007 27.463683215571834 26.980477706777457 22.490026810807702 82-83 22.876240045226183 27.42829274393621 27.020883326582712 22.674583884254897 84-85 22.881383648386436 27.382000193355406 27.046179954938847 22.690436203319315 86-87 22.9163454630597 27.375855479760492 26.946709491418336 22.761089565761473 88-89 22.78120417493315 27.450472986572354 26.637698036171255 23.13062480232324 90-91 22.757643807965632 27.272978867478574 27.024726732157976 22.944650592397814 92-93 22.87820769685524 27.123535299265246 27.110669472231557 22.887587531647956 94-95 22.67368855339981 27.097311878579916 27.120815600721354 23.10818396729892 96-97 22.72875435884738 27.061923934047762 27.161760092059144 23.04756161504572 98-99 22.559843536725527 27.258485695987716 26.806231504981483 23.375439262305274 100-101 22.13344412226843 27.521825947324462 26.773425606332218 23.571304324074884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 88.0 1 76.0 2 85.0 3 105.5 4 91.5 5 92.5 6 142.0 7 299.5 8 510.0 9 658.0 10 695.0 11 676.0 12 721.0 13 841.0 14 964.0 15 1058.0 16 1152.0 17 1302.0 18 1469.0 19 1623.0 20 1788.5 21 2011.0 22 2346.0 23 2765.0 24 3270.0 25 4069.0 26 5223.0 27 6807.0 28 8720.0 29 10979.5 30 13813.0 31 17038.0 32 20587.5 33 23453.0 34 26551.0 35 29788.0 36 33020.5 37 36403.5 38 38840.0 39 40240.5 40 41625.0 41 44415.0 42 47226.0 43 49627.0 44 51876.0 45 53295.0 46 53708.5 47 55693.0 48 61131.0 49 60834.0 50 55153.0 51 51652.5 52 49096.0 53 47901.5 54 46116.0 55 42748.5 56 38678.0 57 34667.5 58 31881.5 59 28332.0 60 23226.0 61 18629.5 62 15255.5 63 12101.5 64 9057.0 65 6785.0 66 5081.5 67 4059.5 68 3052.0 69 2015.0 70 1439.5 71 1067.5 72 881.5 73 739.0 74 581.0 75 436.5 76 312.5 77 200.0 78 126.5 79 90.0 80 65.0 81 42.0 82 25.5 83 18.5 84 12.5 85 7.5 86 4.5 87 1.5 88 0.5 89 1.5 90 1.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03651274532000762 2 0.005893789598898867 3 0.004025027043150446 4 8.625057949608099E-4 5 0.0010781322437010124 6 2.875019316536033E-4 7 0.0 8 0.0 9 0.0017968870728350206 10-11 2.875019316536033E-4 12-13 0.0014734473997247168 14-15 0.002335953194685527 16-17 0.002695330609252531 18-19 0.0 20-21 0.0 22-23 0.0017609493313783201 24-25 0.0010062567607876116 26-27 0.004959408321024657 28-29 0.0057141008916153655 30-31 0.0013296964338979152 32-33 4.6719063893710535E-4 34-35 0.009990692124962714 36-37 0.01218289435382144 38-39 0.009631314710395711 40-41 0.0019765757801185227 42-43 5.750038633072066E-4 44-45 9.343812778742107E-4 46-47 3.593774145670041E-5 48-49 9.703190193309112E-4 50-51 8.625057949608099E-4 52-53 0.0023000154532288263 54-55 0.003342209955473138 56-57 0.0015093851411814173 58-59 8.265680535041095E-4 60-61 0.00434846671626075 62-63 0.005210972511221559 64-65 0.008085991827757594 66-67 0.0023718909361422273 68-69 0.003090645765276235 70-71 7.187548291340082E-5 72-73 0.0013296964338979152 74-75 0.0058578518574421675 76-77 0.0023718909361422273 78-79 0.00348596092129994 80-81 0.004671906389371054 82-83 0.004707844130827754 84-85 0.005354723477048361 86-87 0.003126583506732936 88-89 0.00970319019330911 90-91 0.01214695661236474 92-93 8.984435364175103E-4 94-95 0.0018687625557484214 96-97 0.0011140699851577127 98-99 0.0030187702823628345 100-101 0.0043844044577174505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391295.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73020139600162 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57612352396585 51.172866577634835 2 11.01803476695712 13.823270798387357 3 3.2152759916191718 6.050847314939984 4 1.369843882382528 3.4372233049174703 5 0.7228531257469137 2.267236107891659 6 0.44210066953010985 1.6639838422158568 7 0.28978929949408927 1.2724978783769314 8 0.2222191872249228 1.1151883494940158 9 0.155913813395506 0.8802454423246835 >10 0.8905127683371455 10.20126596764859 >50 0.062098001389405286 2.6676636966746643 >100 0.032595267633976195 3.6451658262998294 >500 0.00218062365839636 0.9640882959125571 >1k 4.590786649255495E-4 0.8384565972815802 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3452 0.24811416701705966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3352 0.24092661872571955 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3072 0.22080148350996734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.12721960475671945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.187548291340082E-5 2 0.0 0.0 0.0 0.0 7.187548291340082E-5 3 0.0 0.0 0.0 0.0 7.187548291340082E-5 4 0.0 0.0 0.0 0.0 7.187548291340082E-5 5 0.0 0.0 0.0 0.0 7.187548291340082E-5 6 7.187548291340082E-5 0.0 0.0 0.0 1.4375096582680164E-4 7 7.187548291340082E-5 0.0 0.0 0.0 1.4375096582680164E-4 8 7.187548291340082E-5 0.0 0.0 0.0 1.4375096582680164E-4 9 7.187548291340082E-5 0.0 0.0 0.0 1.4375096582680164E-4 10-11 7.187548291340082E-5 0.0 0.0 7.187548291340082E-5 1.4375096582680164E-4 12-13 1.7968870728350206E-4 0.0 0.0 7.187548291340082E-5 1.4375096582680164E-4 14-15 2.1562644874020248E-4 0.0 0.0 7.187548291340082E-5 1.4375096582680164E-4 16-17 2.515641901969029E-4 0.0 0.0 1.0781322437010123E-4 1.4375096582680164E-4 18-19 3.2343967311030373E-4 0.0 0.0 1.4375096582680164E-4 1.4375096582680164E-4 20-21 4.3125289748040496E-4 0.0 0.0 2.1562644874020248E-4 1.4375096582680164E-4 22-23 4.3125289748040496E-4 0.0 0.0 3.953151560237045E-4 1.4375096582680164E-4 24-25 4.3125289748040496E-4 0.0 0.0 6.828170876773077E-4 1.4375096582680164E-4 26-27 4.3125289748040496E-4 0.0 0.0 0.001221883209527814 1.4375096582680164E-4 28-29 4.671906389371054E-4 0.0 0.0 0.0019765757801185227 1.4375096582680164E-4 30-31 5.031283803938058E-4 0.0 0.0 0.0032703344725597376 1.4375096582680164E-4 32-33 5.031283803938058E-4 0.0 0.0 0.004959408321024657 2.1562644874020248E-4 34-35 5.390661218505062E-4 0.0 0.0 0.0071875482913400825 2.1562644874020248E-4 36-37 6.468793462206075E-4 0.0 0.0 0.010709446954096723 2.1562644874020248E-4 38-39 6.468793462206075E-4 0.0 0.0 0.01671104977736569 2.1562644874020248E-4 40-41 7.187548291340082E-4 0.0 0.0 0.030295516047998447 2.1562644874020248E-4 42-43 7.187548291340082E-4 0.0 0.0 0.042981538782213696 2.1562644874020248E-4 44-45 7.90630312047409E-4 0.0 0.0 0.056997257950326854 2.515641901969029E-4 46-47 7.90630312047409E-4 0.0 0.0 0.08326774695517486 2.875019316536033E-4 48-49 8.625057949608099E-4 0.0 0.0 0.09929597964486324 2.875019316536033E-4 50-51 8.625057949608099E-4 0.0 0.0 0.11794766746089075 2.875019316536033E-4 52-53 8.625057949608099E-4 0.0 0.0 0.1388634329886904 2.875019316536033E-4 54-55 8.625057949608099E-4 0.0 0.0 0.16869175839775175 2.875019316536033E-4 56-57 8.984435364175103E-4 0.0 0.0 0.23966879777473504 2.875019316536033E-4 58-59 9.343812778742107E-4 0.0 0.0 0.3023801566166773 2.875019316536033E-4 60-61 9.343812778742107E-4 0.0 0.0 0.34748202214483626 2.875019316536033E-4 62-63 9.343812778742107E-4 0.0 0.0 0.40512615944138375 2.875019316536033E-4 64-65 9.343812778742107E-4 0.0 0.0 0.4665078218494281 2.875019316536033E-4 66-67 9.343812778742107E-4 0.0 0.0 0.5709428985225995 2.875019316536033E-4 68-69 9.343812778742107E-4 0.0 0.0 0.6950718575140427 2.875019316536033E-4 70-71 9.703190193309111E-4 0.0 0.0 0.761376990501655 2.875019316536033E-4 72-73 0.0010062567607876116 0.0 0.0 0.8332884111565124 2.875019316536033E-4 74-75 0.0010062567607876116 0.0 0.0 0.9680908793605956 2.875019316536033E-4 76-77 0.0010062567607876116 0.0 0.0 1.1272950740137786 2.875019316536033E-4 78-79 0.0010781322437010124 0.0 0.0 1.2752148178495575 2.875019316536033E-4 80-81 0.0010781322437010124 0.0 0.0 1.3872327579700925 2.875019316536033E-4 82-83 0.0011500077266144132 0.0 0.0 1.5543791934852063 2.875019316536033E-4 84-85 0.0011859454680711134 0.0 0.0 1.847235848615858 2.875019316536033E-4 86-87 0.001221883209527814 0.0 0.0 2.1410628227658406 2.875019316536033E-4 88-89 0.001221883209527814 0.0 0.0 2.497062089635915 2.875019316536033E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5230 0.0 26.532444 1 GTAAGGT 485 0.0 25.464804 4 TACATGG 5420 0.0 24.976013 2 ACATGGG 5195 0.0 24.779495 3 GTATAGC 485 0.0 24.495955 1 TAGCGCG 70 1.7799332E-5 23.751255 28-29 GAGTACT 4740 0.0 20.743649 12-13 CATGGGG 2605 0.0 20.422977 4 GGGCGTA 305 2.0918378E-10 20.248062 9 GTATCAA 9110 0.0 20.13562 1 GTGTAGG 745 0.0 19.774317 1 AGTACTT 4930 0.0 19.5588 12-13 GGTAAGG 560 0.0 19.509607 3 TAAGGTG 610 0.0 19.46789 5 GTACTTT 4995 0.0 19.351135 14-15 TACCTGG 1190 0.0 18.759808 2 GGTATCA 7265 0.0 18.707962 1 GTACCTG 1295 0.0 18.348322 1 ATTCGCG 170 8.931238E-10 18.16109 80-81 AGAGTAC 8115 0.0 17.853363 10-11 >>END_MODULE