FastQCFastQC Report
Thu 26 May 2016
SRR936532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470190
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.19324032948122352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.15861895401274667No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21650.14725987797495563No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15180.10325196063093885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG57900.027.9943471
TACATGG60400.026.0460112
ACATGGG60350.024.7312973
TACCTGG13300.024.3000662
GTACCTG14450.023.6842231
GTATCAA74150.023.654281
GGTATCA55350.022.2421441
CATGGGG31350.021.9856744
GAGTACT36550.021.39161112-13
TCAACGC84950.020.4239044
ATCAACG84950.020.31133
CAACGCA85550.020.2806635
TATCAAC86600.020.2515682
GTACTTT39100.019.81146814-15
AACGCAG88200.019.7791126
ACGCAGA96600.018.0591897
CGCAGAG97600.017.7773558
AGTACTT40550.017.17163712-13
CATGGGA30800.017.1309554
AGAGTAC73000.017.0926110-11
Produced by FastQC (version 0.11.5)