##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936532_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470190 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37569565838429 31.0 31.0 34.0 30.0 34.0 2 31.45765377264163 31.0 31.0 34.0 28.0 34.0 3 31.412222229779825 33.0 31.0 34.0 28.0 34.0 4 35.07867962644284 37.0 35.0 37.0 32.0 37.0 5 35.11280174671301 37.0 35.0 37.0 32.0 37.0 6 35.15912092994783 37.0 35.0 37.0 32.0 37.0 7 35.02861194811555 37.0 35.0 37.0 32.0 37.0 8 35.01138560322135 37.0 35.0 37.0 32.0 37.0 9 36.518664934464255 39.0 37.0 39.0 32.0 39.0 10-11 36.38711391044695 39.0 37.0 39.0 32.0 39.0 12-13 36.294441194675514 39.0 36.0 39.0 32.0 39.0 14-15 37.31058876743822 40.0 37.0 41.0 32.0 41.0 16-17 37.22050687326128 39.5 37.0 41.0 31.5 41.0 18-19 37.07633741217121 39.0 36.0 41.0 31.0 41.0 20-21 37.05767111733857 39.0 36.0 41.0 31.0 41.0 22-23 37.03543895686953 39.0 36.0 41.0 31.0 41.0 24-25 37.15651650466947 39.0 37.0 41.0 31.0 41.0 26-27 37.07886871764874 39.0 36.0 41.0 31.0 41.0 28-29 36.96256742325822 39.0 36.0 41.0 30.5 41.0 30-31 36.79342397921357 39.0 36.0 41.0 30.0 41.0 32-33 36.65259082159449 39.0 36.0 41.0 30.0 41.0 34-35 36.475509287915166 39.0 35.5 40.5 30.0 41.0 36-37 36.27187914487243 39.0 35.0 40.0 30.0 41.0 38-39 36.09194491868398 39.0 35.0 40.0 29.5 41.0 40-41 35.87767159346751 38.5 35.0 40.0 28.5 41.0 42-43 35.638819132221 38.0 35.0 40.0 27.0 41.0 44-45 35.53309028084806 38.0 35.0 40.0 27.0 41.0 46-47 35.45851488583108 38.0 35.0 40.0 27.0 41.0 48-49 35.27386324216598 38.0 34.5 40.0 26.0 41.0 50-51 34.32265387466926 37.5 33.5 39.5 25.0 40.5 52-53 34.4527669893007 38.0 33.5 39.5 25.0 40.5 54-55 34.91018745876383 38.0 34.0 40.0 26.0 41.0 56-57 34.76666315238167 38.0 34.0 40.0 25.5 41.0 58-59 34.551202565654776 38.0 34.0 40.0 25.0 41.0 60-61 34.27192471721342 38.0 34.0 40.0 24.0 41.0 62-63 33.936767696692264 37.0 33.0 40.0 23.0 41.0 64-65 33.57563036070168 36.5 33.0 39.5 22.5 41.0 66-67 33.215923452070825 36.0 32.0 39.0 22.0 41.0 68-69 32.77893741625232 36.0 32.0 39.0 21.5 40.5 70-71 32.376851631421786 35.0 31.5 38.5 20.0 40.0 72-73 31.952015385766465 35.0 31.0 37.5 19.0 40.0 74-75 31.520352811541365 35.0 31.0 37.0 18.0 39.0 76-77 31.050091824866172 35.0 30.5 36.5 15.5 39.0 78-79 30.580272617824907 34.0 30.0 36.0 12.0 38.5 80-81 30.1226052414994 34.0 30.0 35.5 7.5 37.0 82-83 29.713691087546508 34.0 29.0 35.0 7.0 37.0 84-85 29.331856086628257 34.0 29.0 35.0 2.0 36.5 86-87 28.969163849570464 34.0 29.0 35.0 2.0 36.0 88-89 28.612325617777294 34.0 29.0 35.0 2.0 36.0 90-91 28.250113590760378 34.0 28.5 35.0 2.0 35.0 92-93 27.81835034927458 34.0 27.0 35.0 2.0 35.0 94-95 27.320699365388144 33.0 26.0 35.0 2.0 35.0 96-97 26.721967908909733 33.0 25.0 35.0 2.0 35.0 98-99 25.969342738013452 33.0 24.5 35.0 2.0 35.0 100-101 23.414438950067677 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44022638404526404 1101 2 0.43413498894199165 1101 3 0.5647232866439822 1101 4 0.6788975709475338 1101 5 0.5963881166423661 1101 6 0.5854864424339326 1101 7 0.5724635193551251 1101 8 0.5946570182226552 1101 9 0.6457878783868409 1101 10-11 0.7924549261814278 1101 12-13 0.6895183794018251 1101 14-15 0.8849293966173164 1101 16-17 0.9111088064030355 1101 18-19 0.9967518939964535 1101 20-21 0.9896879662041869 1101 22-23 0.8419926465992589 1101 24-25 0.8384857438592803 1101 26-27 0.7170034109121275 1101 28-29 0.7885669654804559 1101 30-31 0.5229138776401001 1101 32-33 0.3387846606072742 1101 34-35 0.5775088525446748 1101 36-37 0.5587183285887249 1101 38-39 0.5239318892656257 1101 40-41 0.4932841788827673 1101 42-43 0.6207129594000378 1101 44-45 0.48508098650736997 1101 46-47 0.4597598250591446 1101 48-49 0.5096509707521051 1101 50-51 0.27531146819436003 1101 52-53 0.23127174221936997 1101 54-55 0.49817864823732094 1101 56-57 0.5254020906930066 1101 58-59 0.25577759598881755 1101 60-61 0.2944804835965229 1101 62-63 0.39540446584386757 1101 64-65 0.2833350857279058 1101 66-67 0.037716117267208915 1101 68-69 0.22468139544110954 1101 70-71 0.3663934905583801 1101 72-73 0.17107691739137465 1101 74-75 0.09448609273676922 1101 76-77 0.161483150971776 1101 78-79 0.018554734050827193 1101 80-81 0.21379890791524758 1101 82-83 0.16810059567713864 1101 84-85 0.20173228710478952 1101 86-87 0.1504672691087734 1101 88-89 0.44778576868301556 1101 90-91 0.2458004012207411 1101 92-93 0.26789929598636064 1101 94-95 0.46361257811341616 1101 96-97 0.3123673040668109 1101 98-99 0.13880937848674435 1101 100-101 0.60699706777595 1102 1 0.2660636113409751 1102 2 0.40681673595526746 1102 3 0.6480122480983255 1102 4 0.303646113376999 1102 5 0.49134190693497715 1102 6 0.38922647399178345 1102 7 0.47375385765770517 1102 8 0.49057521150533745 1102 9 0.5746499072275455 1102 10-11 0.5469802486136928 1102 12-13 0.34756075708470036 1102 14-15 0.5766183507609597 1102 16-17 0.3337780643062018 1102 18-19 0.4307189227457755 1102 20-21 0.4859525038329622 1102 22-23 0.2130861443420784 1102 24-25 0.35383425095370313 1102 26-27 0.20392548495731688 1102 28-29 0.29922291791217503 1102 30-31 0.18285673234605326 1102 32-33 0.4360559423555088 1102 34-35 0.050938423102060426 1102 36-37 0.09035415832256177 1102 38-39 0.11066358053482617 1102 40-41 0.260462188301382 1102 42-43 0.09804156920976936 1102 44-45 0.22975971176522592 1102 46-47 0.2839198562514511 1102 48-49 0.3963330101933238 1102 50-51 0.28793948157294125 1102 52-53 0.17338837223100256 1102 54-55 0.4465072397703125 1102 56-57 0.27460613838313463 1102 58-59 0.060224633023594265 1102 60-61 0.21807728638433588 1102 62-63 0.22051793397836406 1102 64-65 0.251967314942668 1102 66-67 0.053983640097911234 1102 68-69 0.17944888476444731 1102 70-71 -0.12344859835189936 1102 72-73 0.26131926704990605 1102 74-75 0.06642336694279649 1102 76-77 0.2845063685320781 1102 78-79 0.09146494039529429 1102 80-81 0.27459041288347663 1102 82-83 0.0774786989163907 1102 84-85 0.14228747374082928 1102 86-87 0.008144111861739134 1102 88-89 0.1667567269328316 1102 90-91 0.5215870628511468 1102 92-93 0.4031426508659486 1102 94-95 0.5292081258979735 1102 96-97 0.5117270087145904 1102 98-99 0.3343864058348487 1102 100-101 0.5728189426046022 1103 1 0.5488405487596282 1103 2 0.4320685505446846 1103 3 0.4389979307239962 1103 4 0.5470209565425321 1103 5 0.5564830286385245 1103 6 0.42964755254386233 1103 7 0.6058659342046511 1103 8 0.4529167647235255 1103 9 0.41581851453749863 1103 10-11 0.3792213105149358 1103 12-13 0.18639619522449635 1103 14-15 0.6372391220933835 1103 16-17 0.671034018152902 1103 18-19 0.7478722159763862 1103 20-21 0.6117730265754204 1103 22-23 0.46162831668996773 1103 24-25 0.3288503741229931 1103 26-27 0.4420529693911277 1103 28-29 0.2981467696493709 1103 30-31 0.4493736001126152 1103 32-33 0.46356089656570276 1103 34-35 0.46611941300601956 1103 36-37 0.5426553743026687 1103 38-39 0.2685630540400581 1103 40-41 0.5257660074404669 1103 42-43 0.578636308952504 1103 44-45 0.39495213446195265 1103 46-47 0.6250538720345773 1103 48-49 0.5641382657602136 1103 50-51 0.5813621079492677 1103 52-53 0.7495993688187568 1103 54-55 0.6253832496670029 1103 56-57 0.7537600530886408 1103 58-59 0.5307520658632683 1103 60-61 0.6687761524858118 1103 62-63 0.6706252263412509 1103 64-65 0.7358400415758055 1103 66-67 0.5638454498796932 1103 68-69 0.9254518045238314 1103 70-71 0.8040654434628038 1103 72-73 0.9204942298200365 1103 74-75 0.5661296321132347 1103 76-77 0.545375143522989 1103 78-79 0.4572914433678186 1103 80-81 0.5564237051760159 1103 82-83 0.48900676495689055 1103 84-85 0.7052449320257956 1103 86-87 0.5756084356853322 1103 88-89 0.6015552166315956 1103 90-91 0.683171999706957 1103 92-93 0.7025466798993776 1103 94-95 0.7445040300266754 1103 96-97 0.6117050225746539 1103 98-99 0.6434303312109577 1103 100-101 1.1613251749424052 1104 1 0.161630374122943 1104 2 0.30375740299035314 1104 3 0.34607150144225685 1104 4 0.2708458081042622 1104 5 0.33026600926739746 1104 6 0.23387738907276656 1104 7 0.35079606208545755 1104 8 0.2503274546052978 1104 9 0.41884020019146817 1104 10-11 0.3971388713004842 1104 12-13 0.4514736564502684 1104 14-15 0.45735054529802 1104 16-17 0.3702310557844015 1104 18-19 0.3808702910765618 1104 20-21 0.40337964916130176 1104 22-23 0.1615578048759616 1104 24-25 0.3335727756536073 1104 26-27 -0.03369242044469445 1104 28-29 -0.06801352042315045 1104 30-31 -0.07774524355178869 1104 32-33 0.04578028862302119 1104 34-35 0.11692106690787085 1104 36-37 0.010647953842898517 1104 38-39 -0.2788006764274442 1104 40-41 -0.03650755513621817 1104 42-43 0.14316145186613483 1104 44-45 0.25802589708866464 1104 46-47 0.5165848943250282 1104 48-49 0.33613969635042196 1104 50-51 0.10939164440315352 1104 52-53 0.11512999566151194 1104 54-55 0.242387355344583 1104 56-57 0.3393478744625824 1104 58-59 0.3321640467654703 1104 60-61 0.3114333284076949 1104 62-63 0.1939961853600849 1104 64-65 0.21710541605895628 1104 66-67 0.17611087088610589 1104 68-69 0.21679716993494935 1104 70-71 0.08234126241754325 1104 72-73 0.45564429640646154 1104 74-75 0.5668353317939641 1104 76-77 0.38870487130377285 1104 78-79 0.123000893986287 1104 80-81 0.15912568881146072 1104 82-83 -0.013451262340630876 1104 84-85 0.15557975410340674 1104 86-87 0.19462234613968832 1104 88-89 0.2584877637218561 1104 90-91 0.40396048171117727 1104 92-93 0.3750166869360214 1104 94-95 0.5631299074262586 1104 96-97 0.21602427099140797 1104 98-99 -0.05268715746353436 1104 100-101 0.5080089389066522 1105 1 0.13866469141117577 1105 2 0.16955787390925536 1105 3 0.23462255777303298 1105 4 0.16655539673582354 1105 5 0.14704312785810458 1105 6 0.07058440536425081 1105 7 0.1867971708222882 1105 8 0.17534588941492757 1105 9 0.1438784675017999 1105 10-11 0.3034645856971707 1105 12-13 0.16613311122716112 1105 14-15 0.4295525296073919 1105 16-17 0.2818232701448977 1105 18-19 0.28885528546519623 1105 20-21 0.3554407579805243 1105 22-23 0.2115408560326344 1105 24-25 0.08372711017315737 1105 26-27 0.17610821878563598 1105 28-29 0.1743378726481879 1105 30-31 -0.09757171211315807 1105 32-33 0.020169880150014308 1105 34-35 -0.20925353795750112 1105 36-37 0.035656766652927274 1105 38-39 -0.17167148092665485 1105 40-41 -0.26356349985474736 1105 42-43 -0.37233541112658486 1105 44-45 0.0013976046434294176 1105 46-47 0.10730725638174476 1105 48-49 0.037131350810589936 1105 50-51 0.0349275191606182 1105 52-53 0.17654836667757934 1105 54-55 0.07488334912059003 1105 56-57 -0.06152625624713437 1105 58-59 0.04333177499076868 1105 60-61 0.06923656410666723 1105 62-63 0.05415395672842038 1105 64-65 0.12660138806041488 1105 66-67 0.07753289649652118 1105 68-69 0.22327289336513445 1105 70-71 0.12727761892233502 1105 72-73 0.04698223103688548 1105 74-75 -0.01270079366513599 1105 76-77 -0.01820846184516256 1105 78-79 0.06327269929115786 1105 80-81 0.11425392034678339 1105 82-83 -0.10734241074835182 1105 84-85 0.024525765563886637 1105 86-87 0.16979420329154493 1105 88-89 -0.028022281116072634 1105 90-91 -0.05922418829543119 1105 92-93 -0.051917908772271204 1105 94-95 0.0438938529992825 1105 96-97 0.1102211165056417 1105 98-99 0.22898937739252645 1105 100-101 0.4203222147355419 1106 1 0.03696630536434142 1106 2 -0.03140079361254777 1106 3 0.01829722574643 1106 4 0.15428080130504895 1106 5 0.16408824119994136 1106 6 -0.04777536278761829 1106 7 0.1701363892356227 1106 8 0.1068297462801695 1106 9 0.2085729117460744 1106 10-11 0.26853093096640634 1106 12-13 0.3571093619267671 1106 14-15 0.2195203298125321 1106 16-17 0.05878414277015054 1106 18-19 0.3564084973977373 1106 20-21 0.12645649876797904 1106 22-23 -0.04277957336201865 1106 24-25 -0.2430128317447 1106 26-27 -0.19731361894469046 1106 28-29 -0.04958341620834972 1106 30-31 -0.09647486531499538 1106 32-33 -0.07308191479938841 1106 34-35 -0.16068931732225877 1106 36-37 -0.32952115492897605 1106 38-39 -0.2263258585854544 1106 40-41 -0.23684703820504893 1106 42-43 -0.2686577165406234 1106 44-45 -0.5218889963505475 1106 46-47 -0.15682143950854055 1106 48-49 -0.11737031502736528 1106 50-51 -0.31073715858921247 1106 52-53 -0.06443965418203135 1106 54-55 -0.17421797514250414 1106 56-57 0.08004895147217184 1106 58-59 -0.13526659111886374 1106 60-61 -0.10856131101913746 1106 62-63 -0.00684219514987916 1106 64-65 -0.265099928516058 1106 66-67 -0.33705357454528695 1106 68-69 -0.14589156939912584 1106 70-71 -0.31817339439235326 1106 72-73 -0.24034569042492038 1106 74-75 0.04993117024611138 1106 76-77 -0.171125960229773 1106 78-79 -0.02484556439405594 1106 80-81 0.013327500661308278 1106 82-83 -0.05643046355553594 1106 84-85 0.13122406753814886 1106 86-87 0.3065046117160932 1106 88-89 0.366133654287367 1106 90-91 0.4199981764498162 1106 92-93 0.48548657878138357 1106 94-95 0.462730192527566 1106 96-97 0.46320220978536497 1106 98-99 0.6177520573378565 1106 100-101 1.0604510739668314 1107 1 0.12755440198021262 1107 2 0.21134265709721234 1107 3 0.21486701935537766 1107 4 0.23198285235629612 1107 5 0.16927837957981495 1107 6 0.16521319961906755 1107 7 0.2018380259330783 1107 8 0.23115910038834642 1107 9 0.24250884407781115 1107 10-11 0.31914371020871357 1107 12-13 0.2265682730335996 1107 14-15 0.014032106747315254 1107 16-17 0.15445521730381984 1107 18-19 0.2860442589871468 1107 20-21 0.12300593188912501 1107 22-23 -0.04484330526083369 1107 24-25 -0.040062525622985845 1107 26-27 0.03098013645798403 1107 28-29 0.10143782299523707 1107 30-31 0.0428149823028221 1107 32-33 0.12457109248037312 1107 34-35 0.2415187239988228 1107 36-37 0.13395230856860252 1107 38-39 0.06203401482233062 1107 40-41 0.16564229932897234 1107 42-43 0.036679946075381054 1107 44-45 -0.016004993978732784 1107 46-47 0.0678627943298693 1107 48-49 0.014421872260548696 1107 50-51 0.07910456540829358 1107 52-53 0.11866364135938312 1107 54-55 0.2996768432919481 1107 56-57 0.3314795436408815 1107 58-59 0.48619800663648505 1107 60-61 0.33466507617304586 1107 62-63 0.44091440057656683 1107 64-65 0.3945709459847535 1107 66-67 0.4625152831956285 1107 68-69 0.4258041377527704 1107 70-71 0.4934197601973267 1107 72-73 0.4469949083877438 1107 74-75 0.3894271231526609 1107 76-77 0.5569177540947265 1107 78-79 0.5854850613516014 1107 80-81 0.3018329336967369 1107 82-83 0.3787160247961232 1107 84-85 0.5094772805660028 1107 86-87 0.4863341551410869 1107 88-89 0.4569298208004362 1107 90-91 0.26017638429824785 1107 92-93 0.463549046186742 1107 94-95 0.390568337365643 1107 96-97 0.6264308116270918 1107 98-99 0.8270516208375724 1107 100-101 1.2869802537606532 1108 1 0.173845298180197 1108 2 0.12341320649665732 1108 3 0.26847663432215896 1108 4 0.25775274726406394 1108 5 0.25513419319644726 1108 6 0.42667061573015985 1108 7 0.38924106243900525 1108 8 0.36060512674335854 1108 9 0.03623342391855289 1108 10-11 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-6.761447251944563 2316 98-99 -7.7185556023074895 2316 100-101 -8.861010935121241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3772.0 3 1444.0 4 1573.0 5 1578.0 6 1690.0 7 2022.0 8 2474.0 9 2984.0 10 3758.0 11 4685.0 12 5544.0 13 6458.0 14 7272.0 15 8471.0 16 9884.0 17 11977.0 18 10140.0 19 8623.0 20 9206.0 21 9915.0 22 10864.0 23 12642.0 24 14603.0 25 16827.0 26 19791.0 27 23411.0 28 27592.0 29 33325.0 30 40451.0 31 50061.0 32 62755.0 33 80170.0 34 106992.0 35 146009.0 36 206071.0 37 269022.0 38 207701.0 39 28424.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31377140188926 18.230046571273377 14.036726098018848 23.41945592881851 2 15.543355794561409 19.320012627637105 40.61601913740176 24.52061244039973 3 19.88731719827724 23.4516829289365 29.236710756091806 27.424289116694457 4 12.684075394887047 15.859839419176906 37.01849223832143 34.43759294761462 5 14.0066115005276 36.81397517999977 34.33245139172835 14.846961927744287 6 32.89301530646611 35.03994840012491 17.646966772374746 14.420069521034229 7 29.687123488337587 31.664962650043087 20.24814203194112 18.39977182967821 8 26.684088565857838 34.303651453129305 19.66217848599613 19.350081495016724 9 26.223328804754736 16.75054519911679 20.242269879688642 36.78385611643983 10-11 25.173854750124207 25.942538435885744 27.555280297822815 21.32832651616723 12-13 26.07178556191469 23.198233293209775 27.902828404758484 22.82715274011705 14-15 22.755829698631626 24.94022304933895 25.970788565829494 26.333158686199926 16-17 22.500564821040197 28.21631557027871 26.60603119989765 22.677088408783444 18-19 22.32467666796866 26.87629887407097 28.68250788340857 22.1165165745518 20-21 23.02887006260838 26.047106973048393 28.59862129582953 22.325401668513692 22-23 22.666651700802444 26.002338756418315 28.619970449220823 22.71103909355842 24-25 22.663299062003045 26.204012211818934 28.665527123674433 22.467161602503595 26-27 22.433474174267438 26.384356621355142 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22.821152984796715 52-53 22.895269176459067 26.844526006197846 27.43887176333911 22.82133305400398 54-55 22.493370739018484 26.5720709583444 27.705226513779035 23.22933178885808 56-57 22.667710098673705 26.954147829283915 27.46032118524081 22.91782088680157 58-59 22.903129809286547 26.613837141083742 27.463145454081676 23.019887595548035 60-61 22.727550997502927 26.55914037007838 27.983376878501836 22.729931753916855 62-63 22.601200420092617 26.69288617775577 27.917657493918995 22.788255908232617 64-65 22.422204544936676 26.85599878382694 27.735989778160647 22.98580689307574 66-67 22.389249519873513 26.99757955713752 27.440006720240405 23.173164202748563 68-69 22.407561526167985 26.69535580781654 27.82549664943799 23.071586016577488 70-71 22.480071774940125 26.725860132734663 27.615258568677643 23.17880952364757 72-73 22.50723434305158 26.638301896355426 27.69735461326071 23.15710914733228 74-75 22.333574837324953 27.090353106047466 27.500569745332342 23.075502311295242 76-77 22.547852342509426 26.78996403675517 27.424321385952428 23.237862234782973 78-79 22.53353107853909 26.813059347372793 27.513784904466615 23.139624669621504 80-81 22.57793946880959 26.9851274031152 27.41954355692001 23.017389571155203 82-83 22.477911682099634 26.9412517745138 27.51317748675109 23.067659056635474 84-85 22.51651800153523 26.837641487519715 27.397418678580284 23.248421832364773 86-87 22.534386411335042 26.874473061384546 27.48709074788468 23.104049779395737 88-89 22.475435655548562 26.73933175732458 27.244375563497954 23.54085702362891 90-91 22.311705626468857 26.71101724026515 27.60146384101816 23.375813292247834 92-93 22.468156489566105 26.632837666717347 27.722365472243794 23.17664037147276 94-95 22.288019025669144 26.5614477141034 27.689124049158714 23.461409211068737 96-97 22.146256713684707 26.532944545329933 27.80593841589863 23.514860325086723 98-99 22.108888662108377 26.65139299928564 27.49450624213355 23.74521209647243 100-101 22.0842463754411 26.431982246447554 27.7324422087716 23.751329169339748 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 45.0 2 51.5 3 86.0 4 88.0 5 95.5 6 142.0 7 278.5 8 458.5 9 574.5 10 591.0 11 573.5 12 629.0 13 715.5 14 825.5 15 942.5 16 1025.0 17 1089.5 18 1162.0 19 1323.5 20 1547.5 21 1792.0 22 2097.0 23 2533.0 24 3122.0 25 3976.5 26 5247.5 27 6964.5 28 8855.0 29 11215.5 30 14113.0 31 17605.0 32 21478.5 33 24479.5 34 27499.5 35 30787.0 36 34302.5 37 38095.0 38 40476.5 39 42140.5 40 43986.5 41 46859.0 42 49834.0 43 52360.0 44 54831.0 45 56942.5 46 57853.5 47 59325.5 48 64559.5 49 64388.0 50 58621.5 51 55251.5 52 52727.5 53 51325.0 54 49621.0 55 45987.5 56 42043.0 57 37748.5 58 33930.0 59 29777.0 60 24808.5 61 20255.0 62 16318.5 63 12943.0 64 9784.0 65 7354.0 66 5541.5 67 4307.5 68 3214.0 69 2225.0 70 1647.5 71 1221.5 72 927.0 73 736.5 74 569.5 75 437.0 76 320.0 77 194.5 78 120.5 79 67.5 80 35.5 81 28.0 82 22.5 83 17.0 84 15.5 85 13.0 86 7.0 87 6.5 88 6.0 89 5.5 90 3.0 91 1.5 92 2.5 93 2.0 94 1.0 95 1.0 96 2.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07101122984104095 2 0.027071330916412166 3 0.01516810752351737 4 0.01877308375107979 5 0.022242023139866278 6 0.02849971772355954 7 0.07563648235942293 8 0.05271427502567696 9 0.06815445622674621 10-11 0.05849584067365443 12-13 0.03829437011542726 14-15 0.038090314857263344 16-17 0.047476856732803246 18-19 0.04407593576340473 20-21 0.0176167706214843 22-23 0.012107278651058706 24-25 0.020575571864861003 26-27 0.022344050768948232 28-29 0.005679538018895517 30-31 0.0012243315489834648 32-33 0.008774376101048165 34-35 0.009896680020949672 36-37 0.003400920969398513 38-39 0.00775409981022861 40-41 0.0037410130663383646 42-43 0.0018705065331691823 44-45 3.060828872458662E-4 46-47 4.421197260218067E-4 48-49 8.162210326556432E-4 50-51 9.862670811255688E-4 52-53 1.3603683877594052E-4 54-55 2.3806446785789591E-4 56-57 0.002822764404600766 58-59 0.0035709670178684385 60-61 0.004693270937769948 62-63 0.0025846999367428697 64-65 3.060828872458662E-4 66-67 3.060828872458662E-4 68-69 0.0043191696311361116 70-71 0.00265271835613084 72-73 0.019827369251593334 74-75 0.015984328556173013 76-77 0.024214557302117412 78-79 0.019385249525571524 80-81 0.011631149715342915 82-83 0.004693270937769948 84-85 0.0035029485984804684 86-87 0.0015984328556173013 88-89 0.0013943775974533904 90-91 0.005781565647977473 92-93 0.010168753698501554 94-95 0.011971241812282766 96-97 0.010304790537277494 98-99 0.02312626259190989 100-101 0.019521286364347468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1470190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.20152343443786 #Duplication Level Percentage of deduplicated Percentage of total 1 83.05067128394649 54.15030289966032 2 10.232409699605345 13.343374016391744 3 2.9196333995539256 5.710936365629482 4 1.2273761395041909 3.2010717649100946 5 0.6534101374848289 2.1301668195758166 6 0.40054475979414883 1.5669677125355688 7 0.27798037069701353 1.2687320558020538 8 0.18849826724898885 0.98322993515087 9 0.1538072132208602 0.9025618155484946 >10 0.8066959489885278 9.647745501167249 >50 0.06037544045101034 2.676728205976929 >100 0.027026108103491587 3.250632232920276 >500 8.379900806173083E-4 0.3316540553667191 >1k 7.332413205401448E-4 0.8358966193643482 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2841 0.19324032948122352 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2332 0.15861895401274667 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2165 0.14725987797495563 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1518 0.10325196063093885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.3806446785789594E-4 0.0 0.0 3.400920969398513E-5 0.0 16-17 3.7410130663383643E-4 0.0 0.0 2.040552581639108E-4 0.0 18-19 4.081105163278216E-4 0.0 0.0 2.7207367755188104E-4 6.801841938797026E-5 20-21 4.081105163278216E-4 0.0 0.0 3.060828872458662E-4 1.3603683877594052E-4 22-23 4.081105163278216E-4 0.0 0.0 4.081105163278216E-4 1.3603683877594052E-4 24-25 4.4211972602180673E-4 0.0 0.0 6.121657744917324E-4 1.3603683877594052E-4 26-27 4.7612893571579183E-4 0.0 0.0 9.182486617375985E-4 2.040552581639108E-4 28-29 5.101381454097769E-4 0.0 0.0 0.001802488113781212 2.040552581639108E-4 30-31 5.441473551037621E-4 0.0 0.0 0.0031628565015406175 2.040552581639108E-4 32-33 5.441473551037621E-4 0.0 0.0 0.004897326195933859 2.040552581639108E-4 34-35 5.441473551037621E-4 0.0 0.0 0.007516035342370714 2.040552581639108E-4 36-37 5.441473551037621E-4 0.0 0.0 0.011018983940851183 2.040552581639108E-4 38-39 5.441473551037621E-4 0.0 0.0 0.017208660105156474 2.7207367755188104E-4 40-41 5.441473551037621E-4 0.0 0.0 0.02751345064243397 2.7207367755188104E-4 42-43 5.441473551037621E-4 0.0 0.0 0.03720607540521973 2.7207367755188104E-4 44-45 6.461749841857175E-4 0.0 0.0 0.0491773172175025 2.7207367755188104E-4 46-47 7.141934035736878E-4 0.0 0.0 0.07369795740686577 2.7207367755188104E-4 48-49 7.482026132676729E-4 0.0 0.0 0.08981832280181473 2.7207367755188104E-4 50-51 7.482026132676729E-4 0.0 0.0 0.10784320393962685 2.7207367755188104E-4 52-53 7.482026132676729E-4 0.0 0.0 0.12668430611009462 2.7207367755188104E-4 54-55 7.482026132676729E-4 0.0 0.0 0.1534835633489549 2.7207367755188104E-4 56-57 7.482026132676729E-4 0.0 0.0 0.21854318149354846 2.7207367755188104E-4 58-59 7.482026132676729E-4 0.0 0.0 0.27860344581312624 2.7207367755188104E-4 60-61 8.162210326556432E-4 0.0 0.0 0.32587624728776554 2.7207367755188104E-4 62-63 8.162210326556432E-4 0.0 0.0 0.3821274801216169 2.7207367755188104E-4 64-65 8.162210326556432E-4 0.0 0.0 0.44126949577945707 2.7207367755188104E-4 66-67 8.162210326556432E-4 0.0 0.0 0.535270951373632 2.7207367755188104E-4 68-69 8.842394520436134E-4 0.0 0.0 0.6524666879791048 2.7207367755188104E-4 70-71 8.842394520436134E-4 0.0 0.0 0.7149416061869555 2.7207367755188104E-4 72-73 9.182486617375985E-4 0.0 0.0 0.7838782742366632 2.7207367755188104E-4 74-75 9.522578714315837E-4 0.0 0.0 0.9051891252151083 2.7207367755188104E-4 76-77 9.522578714315837E-4 0.0 0.0 1.056802182030894 2.7207367755188104E-4 78-79 9.522578714315837E-4 0.0 0.0 1.2034158850216639 2.7207367755188104E-4 80-81 0.0011223039199015094 0.0 0.0 1.3128235126072139 2.7207367755188104E-4 82-83 0.0012243315489834648 0.0 0.0 1.4746393323311953 2.7207367755188104E-4 84-85 0.00125834075867745 0.0 0.0 1.7465089546249124 2.7207367755188104E-4 86-87 0.001292349968371435 0.0 0.0 2.0501091695631177 2.7207367755188104E-4 88-89 0.001292349968371435 0.0 0.0 2.4065256871560816 2.7207367755188104E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5790 0.0 27.994347 1 TACATGG 6040 0.0 26.046011 2 ACATGGG 6035 0.0 24.731297 3 TACCTGG 1330 0.0 24.300066 2 GTACCTG 1445 0.0 23.684223 1 GTATCAA 7415 0.0 23.65428 1 GGTATCA 5535 0.0 22.242144 1 CATGGGG 3135 0.0 21.985674 4 GAGTACT 3655 0.0 21.391611 12-13 TCAACGC 8495 0.0 20.423904 4 ATCAACG 8495 0.0 20.3113 3 CAACGCA 8555 0.0 20.280663 5 TATCAAC 8660 0.0 20.251568 2 GTACTTT 3910 0.0 19.811468 14-15 AACGCAG 8820 0.0 19.779112 6 ACGCAGA 9660 0.0 18.059189 7 CGCAGAG 9760 0.0 17.777355 8 AGTACTT 4055 0.0 17.171637 12-13 CATGGGA 3080 0.0 17.130955 4 AGAGTAC 7300 0.0 17.09261 10-11 >>END_MODULE