FastQCFastQC Report
Thu 26 May 2016
SRR936532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470190
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37090.2522803175099817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.2203116603976357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30360.2065039212618777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.13413232303307734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC753.0801378E-431.6644333
CGTATAG700.00837850327.1520251
GTACATG57700.025.77561
TACCTGG13300.025.002492
TACATGG59600.023.9914822
ACATGGG57550.023.2737713
GTATCAA87900.023.1904261
GTACCTG13750.023.1532731
GAGTACT47450.022.0201312-13
GGTATCA67000.021.9850351
TGAACCG2056.843238E-720.850065
GTGTAGG9500.020.5069241
GTACTTT50600.020.2738714-15
TATAACG1200.00476137819.7936382
CAACGCA102700.019.4684035
ATCAACG103250.019.3666733
TCAACGC103150.019.337434
AGAGTAC81150.019.28348510-11
AGTACTT50800.019.25914212-13
CATGGGA30450.019.1838824
Produced by FastQC (version 0.11.5)