##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470190 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02182847114999 31.0 31.0 34.0 30.0 34.0 2 32.19231867989852 33.0 31.0 34.0 30.0 34.0 3 32.28314707622824 34.0 31.0 34.0 30.0 34.0 4 35.95385562410301 37.0 35.0 37.0 35.0 37.0 5 35.803846441616386 37.0 35.0 37.0 35.0 37.0 6 35.889412933022264 37.0 35.0 37.0 35.0 37.0 7 35.837121052380986 37.0 35.0 37.0 35.0 37.0 8 35.8041450424775 37.0 35.0 37.0 33.0 37.0 9 37.46816533917385 39.0 37.0 39.0 35.0 39.0 10-11 37.357166420666715 39.0 37.0 39.0 34.0 39.0 12-13 37.26565579959053 39.0 37.0 39.0 34.0 39.0 14-15 38.447411218958095 40.0 38.0 41.0 34.0 41.0 16-17 38.34612090954231 40.0 38.0 41.0 33.0 41.0 18-19 38.46138254239248 40.0 38.0 41.0 34.0 41.0 20-21 38.47060822070617 40.0 38.0 41.0 34.0 41.0 22-23 38.39694903379835 40.0 38.0 41.0 34.0 41.0 24-25 38.26569286962909 40.0 38.0 41.0 34.0 41.0 26-27 38.08946564729729 40.0 37.5 41.0 33.0 41.0 28-29 38.018388439589444 40.0 37.0 41.0 33.0 41.0 30-31 38.02971656724641 40.0 37.0 41.0 33.0 41.0 32-33 37.90576013984587 40.0 37.0 41.0 33.0 41.0 34-35 37.75403859365116 40.0 37.0 41.0 33.0 41.0 36-37 37.57256987192132 40.0 37.0 41.0 33.0 41.0 38-39 37.427687577796064 40.0 37.0 41.0 32.0 41.0 40-41 37.25632469272679 39.5 36.0 41.0 32.0 41.0 42-43 37.01292179922323 39.0 36.0 41.0 31.0 41.0 44-45 36.75885565811222 39.0 36.0 41.0 31.0 41.0 46-47 36.42928294982281 39.0 35.5 40.0 30.0 41.0 48-49 36.09142695842034 39.0 35.0 40.0 29.5 41.0 50-51 35.82751175018195 39.0 35.0 40.0 29.0 41.0 52-53 35.4627027799128 38.0 35.0 40.0 28.0 41.0 54-55 35.29589644875833 38.0 34.0 40.0 28.0 41.0 56-57 35.00487590039383 38.0 34.0 40.0 27.0 41.0 58-59 34.642328882661424 38.0 34.0 40.0 26.0 41.0 60-61 34.226633632387646 37.0 33.0 40.0 26.0 41.0 62-63 33.90409878995232 37.0 33.0 39.5 25.5 41.0 64-65 33.48868411565852 36.0 32.5 39.0 24.5 40.0 66-67 33.0699715683007 36.0 32.0 39.0 23.5 40.0 68-69 32.708409457281036 35.0 32.0 38.0 23.0 40.0 70-71 32.3518779885593 35.0 31.0 38.0 22.0 40.0 72-73 31.89491426278236 35.0 31.0 37.0 21.5 39.0 74-75 31.38591134479217 34.5 30.5 36.5 20.0 39.0 76-77 29.89957862589189 33.0 28.5 35.0 18.5 37.5 78-79 30.657188186560923 34.0 30.0 36.0 18.5 37.5 80-81 30.407903400240784 34.0 30.0 35.0 17.5 37.0 82-83 30.1235275712663 34.0 30.0 35.0 16.5 37.0 84-85 29.75764935144437 34.0 29.5 35.0 12.0 36.0 86-87 29.375877267564057 34.0 29.0 35.0 7.0 36.0 88-89 28.921509804855155 34.0 29.0 35.0 4.5 35.5 90-91 28.458580183513696 33.0 29.0 35.0 2.0 35.0 92-93 27.828058958365926 33.0 27.0 35.0 2.0 35.0 94-95 27.30362946285854 33.0 26.0 34.0 2.0 35.0 96-97 26.614393037634592 32.0 25.0 34.0 2.0 35.0 98-99 25.74628245328835 32.0 24.5 34.0 2.0 35.0 100-101 22.486549697658127 28.0 9.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2451971411906264 1101 2 0.21863470609036284 1101 3 0.21157781291739042 1101 4 0.2286684709561797 1101 5 0.2446520025368102 1101 6 0.19281504941971406 1101 7 0.2122137940716442 1101 8 0.1258405980267696 1101 9 0.09600873379861952 1101 10-11 0.24838575533534168 1101 12-13 0.17007136495591624 1101 14-15 0.3042413410853868 1101 16-17 0.30195703280649155 1101 18-19 0.21856333030752495 1101 20-21 0.26239104805726754 1101 22-23 0.23136867537372297 1101 24-25 0.1418857122848607 1101 26-27 0.16349725111659552 1101 28-29 0.17099014604890783 1101 30-31 0.25827425097567414 1101 32-33 0.17332689485092345 1101 34-35 0.26494006399244796 1101 36-37 0.191823537513919 1101 38-39 0.20679879269143697 1101 40-41 0.1471967240574159 1101 42-43 0.20413225891276454 1101 44-45 0.25303756886189177 1101 46-47 0.22571708229572351 1101 48-49 0.1438017497142141 1101 50-51 0.12213457466845057 1101 52-53 0.16402375130407876 1101 54-55 0.23400463512391667 1101 56-57 0.04607681360402438 1101 58-59 0.08283682394625203 1101 60-61 0.14046108544605573 1101 62-63 0.09636708715714803 1101 64-65 0.18334103564698978 1101 66-67 0.05304579430235634 1101 68-69 -0.14927728077306313 1101 70-71 -0.12280459318019865 1101 72-73 -0.13839647934945631 1101 74-75 0.0018298990135825477 1101 76-77 -0.2567708396183015 1101 78-79 -0.048431215638139236 1101 80-81 -0.03027533006425287 1101 82-83 -0.008369789158312102 1101 84-85 0.0017203002784356158 1101 86-87 -0.10828871149432118 1101 88-89 -0.019284016813728044 1101 90-91 0.05602990917331141 1101 92-93 -0.07817992536843832 1101 94-95 0.058503329318600805 1101 96-97 0.10468138746980671 1101 98-99 0.08432069900726802 1101 100-101 0.4291215613902608 1102 1 0.215031371099915 1102 2 0.21730172204596698 1102 3 0.2221429908415189 1102 4 0.23001059337382657 1102 5 0.2686700843313403 1102 6 0.17711471495525188 1102 7 0.17960137685007282 1102 8 0.17345578722155608 1102 9 0.22766162182917782 1102 10-11 0.26336750905009154 1102 12-13 0.3047085410767352 1102 14-15 0.2871279095043846 1102 16-17 0.33060613755052515 1102 18-19 0.24873245113509768 1102 20-21 0.21063587116881877 1102 22-23 0.1405664480475508 1102 24-25 0.1231581393758745 1102 26-27 0.18375184619527118 1102 28-29 0.1355789497140023 1102 30-31 0.21948277037643749 1102 32-33 0.29981538354675763 1102 34-35 0.2979143607946284 1102 36-37 0.2831222831198872 1102 38-39 0.2675619687026014 1102 40-41 0.2801149721703453 1102 42-43 0.41726623355683046 1102 44-45 0.35556311061512247 1102 46-47 0.31457009460815044 1102 48-49 0.49384652774313054 1102 50-51 0.3172793569619401 1102 52-53 0.5830985823964809 1102 54-55 0.41405739399881014 1102 56-57 0.2962757255283819 1102 58-59 0.4006197936738687 1102 60-61 0.41931763126280686 1102 62-63 0.46723029789411896 1102 64-65 0.31985949820707305 1102 66-67 0.18845759986214006 1102 68-69 0.2234794009008425 1102 70-71 0.18500993439636204 1102 72-73 0.3487047068113931 1102 74-75 0.22075547000960682 1102 76-77 -0.06080177605787185 1102 78-79 0.11880893471856169 1102 80-81 0.1360019403645829 1102 82-83 0.1807387929926172 1102 84-85 0.36825527283285453 1102 86-87 0.2628227583825513 1102 88-89 0.14788729113117682 1102 90-91 0.0031823920693234697 1102 92-93 0.09753332466354436 1102 94-95 -0.075016528366465 1102 96-97 0.08462052590397562 1102 98-99 0.014615624773217206 1102 100-101 0.654614576626976 1103 1 0.10518392559622569 1103 2 0.12661211943811423 1103 3 0.1170562775056041 1103 4 0.0951188824417315 1103 5 0.14899512742417897 1103 6 0.14558131361145854 1103 7 0.0190468570860034 1103 8 0.0515839752118481 1103 9 0.13984546113687912 1103 10-11 0.1323588435794747 1103 12-13 0.1961676585823895 1103 14-15 0.2428774316722766 1103 16-17 0.1472279524917255 1103 18-19 0.09796352253435003 1103 20-21 0.021856717547265703 1103 22-23 0.05990985396446291 1103 24-25 0.059087009815947056 1103 26-27 0.02969085957457196 1103 28-29 0.14999687398787387 1103 30-31 0.1841167451187573 1103 32-33 0.12164791365310634 1103 34-35 0.10087897190378214 1103 36-37 0.010151164113310074 1103 38-39 0.02729948350660294 1103 40-41 0.2895481987976112 1103 42-43 0.18745507984146315 1103 44-45 0.17133215647527322 1103 46-47 0.22440903880440288 1103 48-49 0.052046478494169435 1103 50-51 0.11707210094946419 1103 52-53 0.15680022754604295 1103 54-55 -0.03081485187073696 1103 56-57 0.020423542744410383 1103 58-59 0.14163420485572686 1103 60-61 0.24164147830963145 1103 62-63 0.1500989908034427 1103 64-65 0.0907853199024089 1103 66-67 -0.12987004638966226 1103 68-69 0.08799670252722791 1103 70-71 -0.11741323137326276 1103 72-73 -0.13009918854631053 1103 74-75 -0.13045669917227798 1103 76-77 -0.020217706921723533 1103 78-79 -0.2652165222999976 1103 80-81 -0.20862728537151298 1103 82-83 -0.43355600889760737 1103 84-85 -0.1422831237619313 1103 86-87 0.06954963775779532 1103 88-89 0.04625181546836643 1103 90-91 -0.06363729813130803 1103 92-93 0.04139567383862541 1103 94-95 -0.06889500071083532 1103 96-97 0.006142611714412993 1103 98-99 -0.08504315033495402 1103 100-101 -0.03291664369173475 1104 1 0.04463274128852035 1104 2 0.07204150003907728 1104 3 0.02788165826940059 1104 4 0.08655511359253865 1104 5 0.07919279311966676 1104 6 -0.135149334166762 1104 7 -0.024177611821926348 1104 8 -0.06355847319095176 1104 9 0.02863364655532763 1104 10-11 0.04175828646345536 1104 12-13 0.008596492535069444 1104 14-15 0.11148019282767763 1104 16-17 0.07761592739242218 1104 18-19 -0.1406974508485277 1104 20-21 -0.11499624569354694 1104 22-23 -0.06468626599576055 1104 24-25 -0.008692435045269065 1104 26-27 -0.07530297609364567 1104 28-29 -0.037128541086829614 1104 30-31 -0.13749214547598143 1104 32-33 -0.1708417785373797 1104 34-35 -0.10851712712097594 1104 36-37 -0.12923978827594595 1104 38-39 -0.021488730988885152 1104 40-41 -0.02474447320759765 1104 42-43 -0.17005188410882965 1104 44-45 -0.20267093732142882 1104 46-47 -0.19717208322028767 1104 48-49 0.03162742024124299 1104 50-51 -0.017273258646248735 1104 52-53 -1.6314120312443947E-4 1104 54-55 -0.12206266382371922 1104 56-57 -0.32800716958483633 1104 58-59 -0.2781503924563964 1104 60-61 -0.09319775475444914 1104 62-63 -0.2760501336869794 1104 64-65 -0.32821783204837374 1104 66-67 -0.3820865888973799 1104 68-69 -0.3081898441094282 1104 70-71 -0.35176891331700944 1104 72-73 -0.153690115347775 1104 74-75 -0.2812584984593087 1104 76-77 -0.31571512778856814 1104 78-79 -0.3431312044589099 1104 80-81 -0.31791609013084354 1104 82-83 -0.38637284682802076 1104 84-85 -0.23612591585530396 1104 86-87 -0.1717015180544159 1104 88-89 -0.17342214371910458 1104 90-91 -0.030084433170880942 1104 92-93 0.05302401697091952 1104 94-95 0.04221504424226552 1104 96-97 0.04521182669257939 1104 98-99 -0.11879286683384649 1104 100-101 0.35582269230505403 1105 1 -0.1151198173688961 1105 2 -0.10124541621892291 1105 3 -0.07541801081620747 1105 4 0.0327310602413462 1105 5 0.09941458756668453 1105 6 -0.05144911918716133 1105 7 -0.07771271093440646 1105 8 0.03754873353962296 1105 9 -0.01209212881011723 1105 10-11 -6.884144265413283E-4 1105 12-13 0.08863872928804284 1105 14-15 0.03482139834947162 1105 16-17 -0.06192616547193808 1105 18-19 -0.033194409766998945 1105 20-21 -0.08536975582842388 1105 22-23 -0.08359202395332943 1105 24-25 -0.09278915291352519 1105 26-27 -0.09994309271110069 1105 28-29 -0.11142303077069471 1105 30-31 -0.12100277469590281 1105 32-33 -0.15535256606420944 1105 34-35 -0.04974495311991234 1105 36-37 -0.17617513726617062 1105 38-39 -0.1415899709027002 1105 40-41 -0.08763041985051956 1105 42-43 0.04977240699697205 1105 44-45 0.15823996884729752 1105 46-47 0.07886660722261496 1105 48-49 0.1064481773955066 1105 50-51 0.2072194677694057 1105 52-53 0.23517316680915457 1105 54-55 0.037229539531800526 1105 56-57 0.006823556784915752 1105 58-59 -0.011719136199886293 1105 60-61 0.008379517552846494 1105 62-63 -0.17873640765444065 1105 64-65 -0.08139838842945579 1105 66-67 0.04902180660623401 1105 68-69 0.069989167455212 1105 70-71 0.07755558700107201 1105 72-73 0.16846487711646674 1105 74-75 -0.024996989576759887 1105 76-77 -0.024433954128245006 1105 78-79 -0.1338936140430107 1105 80-81 0.05625301930748705 1105 82-83 0.046304346561225174 1105 84-85 0.11722142185216455 1105 86-87 0.25989415669493 1105 88-89 -0.05649807630272363 1105 90-91 0.05338038120691735 1105 92-93 0.05907881470000831 1105 94-95 0.06216318548969113 1105 96-97 -0.10404348350362014 1105 98-99 -0.25251129593501176 1105 100-101 0.2100420421168252 1106 1 0.011112146061936556 1106 2 0.07226475776325714 1106 3 -0.019830427614387247 1106 4 0.026533110170845475 1106 5 0.04874945342289294 1106 6 -0.10458959004160562 1106 7 -0.08160827358408085 1106 8 -0.06391484982553663 1106 9 -0.06280710597840766 1106 10-11 -0.13517480307722707 1106 12-13 -0.0364891811242245 1106 14-15 0.06117124353583847 1106 16-17 0.06411002897110762 1106 18-19 0.06067982247027004 1106 20-21 -0.020285274904495054 1106 22-23 -0.07079227521021636 1106 24-25 -0.08116792146654461 1106 26-27 -0.336324236914777 1106 28-29 -0.413431524502478 1106 30-31 -0.1406340203871892 1106 32-33 -0.22209808079252014 1106 34-35 -0.12069049331019954 1106 36-37 -0.3774529803002764 1106 38-39 -0.24520461106008185 1106 40-41 0.0596614224677694 1106 42-43 -0.06893956517740207 1106 44-45 -0.007301326398760466 1106 46-47 -0.02346712725698552 1106 48-49 -0.17877492718837118 1106 50-51 -0.09326400956823733 1106 52-53 -0.12720603892123705 1106 54-55 0.07043310084592491 1106 56-57 -0.35489868185147344 1106 58-59 -0.22309199325009388 1106 60-61 -0.26298182155506566 1106 62-63 -0.22064087959461176 1106 64-65 -0.18617473136868767 1106 66-67 -0.1745079442747297 1106 68-69 -0.22415703955066846 1106 70-71 0.040973509351644566 1106 72-73 0.027123271042096775 1106 74-75 -0.1070250962384307 1106 76-77 -0.30618936539925556 1106 78-79 -0.38929257442561394 1106 80-81 -0.31022772687261835 1106 82-83 -0.18846770867685692 1106 84-85 0.09739500769146048 1106 86-87 0.09400666261003465 1106 88-89 -0.0767916937533073 1106 90-91 -0.06687912680039787 1106 92-93 0.160301890459003 1106 94-95 -0.03735081499553061 1106 96-97 0.11619032231756421 1106 98-99 0.0018595770025093827 1106 100-101 0.36084181247859703 1107 1 -0.011014703349037092 1107 2 -0.06719029556693101 1107 3 -0.1239118124133185 1107 4 -0.07042031789233505 1107 5 -0.11561147730144228 1107 6 -0.11819361070214285 1107 7 -0.06350351351635908 1107 8 -0.05253440875307547 1107 9 -0.17057838268815573 1107 10-11 0.01812806096472741 1107 12-13 -0.09209920991467158 1107 14-15 -0.04819940307039161 1107 16-17 0.04210998610692229 1107 18-19 -0.1479276630452162 1107 20-21 -0.2428655916708209 1107 22-23 -0.19763007713481784 1107 24-25 -0.21559893513307316 1107 26-27 -0.15101432885845867 1107 28-29 -0.17965740446847178 1107 30-31 -0.14678592391418732 1107 32-33 -0.2160926298970196 1107 34-35 -0.21772161270839518 1107 36-37 -0.12461412105911762 1107 38-39 -0.153477041530806 1107 40-41 -0.08292718137428778 1107 42-43 -0.12575872118161158 1107 44-45 0.010147968842602495 1107 46-47 -0.08274115084657296 1107 48-49 -0.3320384990608929 1107 50-51 -0.3167284001466797 1107 52-53 -0.012065694436067531 1107 54-55 -0.20301271525243436 1107 56-57 -0.4054855639821042 1107 58-59 -0.22823390942203048 1107 60-61 -0.08339071015819144 1107 62-63 -0.028149434571503207 1107 64-65 -0.22844685464608006 1107 66-67 -0.03938217361240248 1107 68-69 0.06540213802850303 1107 70-71 0.04638779169803797 1107 72-73 0.1834382370722274 1107 74-75 0.17034279277190478 1107 76-77 -0.045850166816631344 1107 78-79 0.08302877122402919 1107 80-81 0.23881068658102222 1107 82-83 0.2379380579848096 1107 84-85 0.46399362746470985 1107 86-87 0.4796977047098103 1107 88-89 0.18170523788545978 1107 90-91 0.11149928536731935 1107 92-93 0.2968877224171109 1107 94-95 0.1826326465731576 1107 96-97 0.25461931422337614 1107 98-99 0.012514005944407103 1107 100-101 0.8945705679169933 1108 1 0.04766274318047792 1108 2 0.059261536209270105 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 16.0 5 37.0 6 74.0 7 168.0 8 430.0 9 864.0 10 1416.0 11 2222.0 12 3200.0 13 4486.0 14 5940.0 15 7957.0 16 9864.0 17 12605.0 18 11068.0 19 7928.0 20 7452.0 21 7875.0 22 8376.0 23 9636.0 24 11318.0 25 13809.0 26 16993.0 27 21285.0 28 26649.0 29 32980.0 30 41516.0 31 51712.0 32 65593.0 33 85590.0 34 114688.0 35 158914.0 36 230191.0 37 303794.0 38 183751.0 39 9784.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67568589912366 19.16367488310103 14.489621113317671 22.671018104457637 2 16.070454325912483 20.378135539951646 39.336409066110114 24.215001068025757 3 20.25615159036969 23.89353682519232 29.386279672967287 26.464031911470702 4 13.374461804256594 16.65995551595372 36.54704493976969 33.41853774002 5 14.111523617382218 37.40995792254647 33.8638475362311 14.614670923840217 6 33.108645821288405 35.75340602235085 16.904481733653473 14.23346642270727 7 29.817234507104523 32.10387773008931 19.940823975132467 18.1380637876737 8 26.98372319224046 34.923445268978845 19.01169236629279 19.08113917248791 9 26.515756466851226 16.891694270808536 19.776423455471743 36.8161258068685 10-11 25.501227732469957 26.449744590835202 26.945530849754114 21.103496826940734 12-13 26.48930450092829 23.449767357548374 27.740652555189875 22.32027558633346 14-15 22.942837524354903 25.349641014932462 25.827879660165564 25.87964180054707 16-17 22.640441031431312 28.62595990994361 26.438963671362202 22.294635387262872 18-19 22.379366390631798 27.135738891804284 28.822726980552176 21.662167737011742 20-21 23.123983974860394 26.155326862514368 28.93564777341704 21.7850413892082 22-23 22.77588119622715 26.14425764306494 28.77653825460536 22.303322906102547 24-25 22.894115726538747 26.34084710139506 28.875689536726547 21.88934763533965 26-27 22.70334999015419 26.515770131110823 28.865194703872376 21.91568517486261 28-29 22.32461550652876 26.785287336619394 28.950374741055068 21.939722415796773 30-31 22.48095708887395 26.382624008913275 28.853058480110928 22.28336042210185 32-33 22.427433184654493 26.61706094789393 28.75445815440061 22.201047713050972 34-35 22.99312833401636 26.49245749208849 28.511275075799187 22.003139098095964 36-37 22.95078231757543 26.262240823081207 28.621164848185565 22.165812011157797 38-39 22.64474547426481 26.501801995748124 28.75816526666374 22.095287263323325 40-41 22.82269046229525 26.515658562930135 28.538123747607447 22.123527227167177 42-43 22.78612149777464 26.639598231686122 28.181192992862396 22.39308727767684 44-45 22.891467513647292 26.62561000868271 28.109084465775407 22.373838011894595 46-47 23.159016328211642 26.958638892847198 27.628316290699374 22.25402848824179 48-49 23.315012466147884 26.95228446283673 27.476165357814665 22.256537713200718 50-51 22.955858056345136 27.11862216354496 27.577882843634388 22.347636936475514 52-53 23.293975528283138 27.25493423625702 27.129916126315905 22.32117410914394 54-55 22.870290069481996 27.04359714725898 27.408352123741214 22.67776065951781 56-57 23.08545822092799 27.288111321512677 27.262906219673518 22.36352423788582 58-59 23.374479296895597 26.98246491366832 27.180727564053498 22.462328225382585 60-61 23.04654054247124 27.007529692706218 27.746211766866967 22.19971799795557 62-63 22.93599482704021 27.0558926604041 27.72530250147709 22.282810011078595 64-65 22.85121429774149 27.205473906927935 27.419878799836038 22.523432995494538 66-67 22.861199773492885 27.332504621119234 27.178541001698804 22.627754603689073 68-69 22.778308704746035 27.091434693148774 27.463057119050454 22.66719948305474 70-71 22.985820190465308 27.19538100203443 27.27717320698223 22.541625600518028 72-73 22.94384499413171 27.0425003035323 27.424559895169974 22.589094807166017 74-75 22.91083542096181 27.504865086938985 26.976490457899736 22.60780903419947 76-77 23.002493609530404 27.214353831356902 27.106134170612954 22.67701838849974 78-79 23.05231611911149 27.21051784839063 27.033311122970243 22.703854909527635 80-81 23.18015234873806 27.439372427150648 26.86869394666502 22.51178127744628 82-83 22.990164697270377 27.373735380897728 26.959798191588728 22.676301730243164 84-85 22.966244951098844 27.33708884603203 26.954064652469928 22.7426015503992 86-87 23.000123468589827 27.293224975756956 26.9169008771712 22.789750678482008 88-89 22.91060541466364 27.374295403377847 26.6432195238463 23.071879658112213 90-91 22.88769427543942 27.23277461582146 26.942839459082602 22.936691649656524 92-93 22.936126566388328 27.189796852169685 26.991700927203233 22.882375654238754 94-95 22.67011268155706 27.160637047812607 27.05463024514243 23.114620025487902 96-97 22.76723644532704 27.03846669493215 27.144394212335506 23.049902647405304 98-99 22.630902857531755 27.190737479689318 26.747756254838407 23.430603407940517 100-101 22.28774640338265 27.40888711173123 26.760670744676325 23.542695740209798 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 121.0 1 97.0 2 79.0 3 105.0 4 105.5 5 105.5 6 170.5 7 332.5 8 528.0 9 646.0 10 691.5 11 706.5 12 765.5 13 891.5 14 1025.0 15 1123.0 16 1239.0 17 1396.0 18 1524.5 19 1668.5 20 1853.0 21 2112.0 22 2418.5 23 2852.0 24 3477.0 25 4269.5 26 5442.5 27 7212.5 28 9161.5 29 11558.5 30 14520.5 31 17923.5 32 21495.0 33 24650.5 34 27836.5 35 31002.0 36 34692.5 37 37979.0 38 40275.5 39 42132.5 40 43786.5 41 46823.5 42 50044.0 43 52636.5 44 54742.5 45 56166.5 46 56718.0 47 58573.0 48 64285.0 49 64005.0 50 58484.0 51 55004.0 52 51958.0 53 50581.0 54 48968.0 55 45502.0 56 41186.5 57 37053.5 58 34007.0 59 30043.0 60 24750.5 61 20264.0 62 16562.5 63 13033.5 64 9782.0 65 7300.5 66 5476.0 67 4394.5 68 3270.5 69 2169.0 70 1590.0 71 1136.5 72 917.0 73 790.5 74 620.0 75 472.0 76 335.0 77 216.5 78 131.5 79 87.5 80 63.0 81 44.0 82 35.5 83 23.5 84 11.5 85 7.0 86 6.0 87 2.0 88 3.0 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022514096817418155 2 0.01278746284493841 3 0.008366265584720343 4 0.0 5 0.0029928104530706915 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010542855005135391 14-15 2.3806446785789591E-4 16-17 0.0 18-19 0.001734469694393242 20-21 0.0 22-23 6.801841938797026E-5 24-25 0.0 26-27 0.0014623960168413605 28-29 0.002822764404600766 30-31 0.0015984328556173013 32-33 0.008910412939824103 34-35 0.001530414436229331 36-37 4.7612893571579183E-4 38-39 9.862670811255688E-4 40-41 2.7207367755188104E-4 42-43 0.006053639325529353 44-45 0.001802488113781212 46-47 3.060828872458662E-4 48-49 5.781565647977472E-4 50-51 6.801841938797026E-5 52-53 2.040552581639108E-4 54-55 0.0017004604846992564 56-57 0.01697059563729858 58-59 0.028737782191417437 60-61 0.023296308640379813 62-63 0.016222393024030907 64-65 0.020915663961800855 66-67 0.002550690727048885 68-69 0.0010202762908195539 70-71 6.801841938797026E-5 72-73 3.400920969398513E-5 74-75 0.0014283868071473754 76-77 0.0021765894204150483 78-79 0.010576864214829377 80-81 0.018569028492915883 82-83 0.019113175848019644 84-85 0.02989409532101293 86-87 0.012277324699528632 88-89 0.043769852876158866 90-91 0.049211326427196486 92-93 0.030438242676116695 94-95 0.032172712370509934 96-97 0.053666532897108536 98-99 0.05628524204354539 100-101 0.06308708398234242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1470190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.28813254702206 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38063116289159 50.69047540634505 2 11.194121020143141 13.945217877001637 3 3.192329124342024 5.9653265889220455 4 1.3358270621637098 3.328246924318089 5 0.7181345096316964 2.23656287612649 6 0.47623925144328155 1.7798432170796215 7 0.30942233204372166 1.3491337461943604 8 0.22177847803768722 1.1051333788870628 9 0.15621266141020881 0.8757175463497947 >10 0.9145991526379573 10.425826544426155 >50 0.06455632533565123 2.750116152840359 >100 0.03352794460542946 3.7607502891446454 >500 0.001965731485371098 0.8307672283763269 >1k 6.552438284570326E-4 0.9568822239883559 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3709 0.2522803175099817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3239 0.2203116603976357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3036 0.2065039212618777 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1972 0.13413232303307734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.801841938797026E-5 0.0 0.0 0.0 0.0 7 1.3603683877594052E-4 0.0 0.0 0.0 0.0 8 1.3603683877594052E-4 0.0 0.0 0.0 0.0 9 1.3603683877594052E-4 0.0 0.0 0.0 0.0 10-11 1.3603683877594052E-4 0.0 0.0 0.0 0.0 12-13 1.7004604846992564E-4 0.0 0.0 6.801841938797026E-5 0.0 14-15 2.040552581639108E-4 0.0 0.0 1.020276290819554E-4 0.0 16-17 2.3806446785789591E-4 0.0 0.0 1.7004604846992564E-4 0.0 18-19 2.7207367755188104E-4 0.0 0.0 2.040552581639108E-4 0.0 20-21 2.7207367755188104E-4 0.0 0.0 2.3806446785789591E-4 0.0 22-23 2.7207367755188104E-4 0.0 0.0 3.4009209693985134E-4 0.0 24-25 2.7207367755188104E-4 0.0 0.0 5.441473551037621E-4 0.0 26-27 2.7207367755188104E-4 0.0 0.0 8.162210326556432E-4 0.0 28-29 4.081105163278216E-4 0.0 0.0 0.001734469694393242 0.0 30-31 4.7612893571579183E-4 0.0 0.0 0.0030608288724586616 0.0 32-33 4.7612893571579183E-4 0.0 0.0 0.004659261728075963 0.0 34-35 5.441473551037621E-4 0.0 0.0 0.007209952455124848 0.0 36-37 6.801841938797027E-4 0.0 0.0 0.011121011569933139 0.0 38-39 7.141934035736878E-4 0.0 0.0 0.017412715363320386 0.0 40-41 7.482026132676729E-4 0.0 0.0 0.02795557036845578 0.0 42-43 7.482026132676729E-4 0.0 0.0 0.03781824117971147 0.0 44-45 9.182486617375985E-4 0.0 0.0 0.049687455362912275 0.0 46-47 0.0010202762908195539 0.0 0.0 0.07427611397166353 0.0 48-49 0.0010882947102075242 0.0 0.0 0.09002237805997865 0.0 50-51 0.0010882947102075242 0.0 0.0 0.10811527761717873 0.0 52-53 0.0012243315489834648 0.0 0.0 0.12702439820703448 0.0 54-55 0.0012243315489834648 0.0 0.0 0.15382365544589477 6.801841938797026E-5 56-57 0.0012243315489834648 0.0 0.0 0.21881525517110034 6.801841938797026E-5 58-59 0.0012243315489834648 0.0 0.0 0.2789095287003721 6.801841938797026E-5 60-61 0.0012243315489834648 0.0 0.0 0.32611431175562344 6.801841938797026E-5 62-63 0.0012243315489834648 0.0 0.0 0.38301171957366054 6.801841938797026E-5 64-65 0.0012243315489834648 0.0 0.0 0.4426638733769105 6.801841938797026E-5 66-67 0.0013603683877594054 0.0 0.0 0.537413531584353 6.801841938797026E-5 68-69 0.0013603683877594054 0.0 0.0 0.6553914800127875 6.801841938797026E-5 70-71 0.0013603683877594054 0.0 0.0 0.7187846468823758 6.801841938797026E-5 72-73 0.0013603683877594054 0.0 0.0 0.7878913609805536 6.801841938797026E-5 74-75 0.0013603683877594054 0.0 0.0 0.9078758527809331 6.801841938797026E-5 76-77 0.0013603683877594054 0.0 0.0 1.0584686333058992 6.801841938797026E-5 78-79 0.0013603683877594054 0.0 0.0 1.2046402165706473 1.3603683877594052E-4 80-81 0.0014964052265353457 0.0 0.0 1.3128915310266018 1.3603683877594052E-4 82-83 0.001564423645923316 0.0 0.0 1.4735850468306817 1.3603683877594052E-4 84-85 0.001564423645923316 0.0 0.0 1.7440943007366396 1.3603683877594052E-4 86-87 0.001564423645923316 0.0 0.0 2.0459600459804514 1.7004604846992564E-4 88-89 0.0016324420653112863 0.0 0.0 2.3986355505070773 2.040552581639108E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACGC 75 3.0801378E-4 31.664433 3 CGTATAG 70 0.008378503 27.152025 1 GTACATG 5770 0.0 25.7756 1 TACCTGG 1330 0.0 25.00249 2 TACATGG 5960 0.0 23.991482 2 ACATGGG 5755 0.0 23.273771 3 GTATCAA 8790 0.0 23.190426 1 GTACCTG 1375 0.0 23.153273 1 GAGTACT 4745 0.0 22.02013 12-13 GGTATCA 6700 0.0 21.985035 1 TGAACCG 205 6.843238E-7 20.85006 5 GTGTAGG 950 0.0 20.506924 1 GTACTTT 5060 0.0 20.27387 14-15 TATAACG 120 0.004761378 19.793638 2 CAACGCA 10270 0.0 19.468403 5 ATCAACG 10325 0.0 19.366673 3 TCAACGC 10315 0.0 19.33743 4 AGAGTAC 8115 0.0 19.283485 10-11 AGTACTT 5080 0.0 19.259142 12-13 CATGGGA 3045 0.0 19.183882 4 >>END_MODULE