FastQCFastQC Report
Thu 26 May 2016
SRR936531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899978
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.32856358710990713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.2520061601505814No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20020.2224498821082293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.164337350468567No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14520.16133727713344104No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10580.11755842920604725No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9630.10700261561949291No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9600.10666927413781227No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9160.1017802657398292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11200.039.4467932
GTACCTG12800.035.256261
GTACATG42400.028.4570871
ACCTGGG14750.028.0219333
TACATGG42350.027.146182
ACATGGG42550.025.9035513
CATGGGG15050.023.6779424
CATGGGA26300.023.1245574
GTACACG1703.1459003E-622.3544311
ATGGGAG11400.022.089725
GTATAGG2405.9189915E-921.7722851
GTATCAA66900.020.8048251
GAGTACT30800.020.74465812-13
GTACTAG1856.5457098E-620.5419121
CCCGGAC1404.8447898E-420.3607673
AAGACGG1656.306713E-520.1573435
ATGGGGA8750.020.0915245
GTGCTAT2451.753724E-719.389051
GTGTTAC2752.833076E-819.0012681
GTACTTT34650.018.58092514-15
Produced by FastQC (version 0.11.5)