##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936531_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 899978 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49596434579512 31.0 31.0 34.0 30.0 34.0 2 31.581616439512967 33.0 31.0 34.0 30.0 34.0 3 31.60248028285136 33.0 31.0 34.0 28.0 34.0 4 35.23167677432115 37.0 35.0 37.0 33.0 37.0 5 35.235549091200006 37.0 35.0 37.0 33.0 37.0 6 35.227473338237154 37.0 35.0 37.0 32.0 37.0 7 35.15042701043803 37.0 35.0 37.0 32.0 37.0 8 35.11935402865404 37.0 35.0 37.0 32.0 37.0 9 36.63231434546178 39.0 37.0 39.0 32.0 39.0 10-11 36.51171861978848 39.0 37.0 39.0 32.0 39.0 12-13 36.4153695979235 39.0 37.0 39.0 32.0 39.0 14-15 37.51886490558658 40.0 37.0 41.0 32.0 41.0 16-17 37.423757025171724 40.0 37.0 41.0 32.0 41.0 18-19 37.393202944960876 40.0 37.0 41.0 32.0 41.0 20-21 37.2526783988053 39.5 37.0 41.0 31.5 41.0 22-23 37.366157283844714 40.0 37.0 41.0 32.0 41.0 24-25 37.40958556764721 40.0 37.0 41.0 32.0 41.0 26-27 37.317625541957696 40.0 37.0 41.0 31.5 41.0 28-29 37.17215031923003 39.5 36.5 41.0 31.0 41.0 30-31 36.96620806286376 39.0 36.0 41.0 30.5 41.0 32-33 36.77301111804955 39.0 36.0 41.0 30.0 41.0 34-35 36.576757431848336 39.0 35.5 41.0 30.0 41.0 36-37 36.36426890435099 39.0 35.0 40.5 30.0 41.0 38-39 36.16302731844556 39.0 35.0 40.0 30.0 41.0 40-41 35.95250772796668 38.5 35.0 40.0 29.0 41.0 42-43 35.71939258515208 38.0 35.0 40.0 28.0 41.0 44-45 35.459827906904394 38.0 35.0 40.0 27.0 41.0 46-47 35.276084526510644 38.0 35.0 40.0 26.5 41.0 48-49 35.12763367548985 38.0 35.0 40.0 26.0 41.0 50-51 34.20163048430072 37.5 33.5 39.5 24.5 40.5 52-53 34.32435348419628 38.0 33.5 39.5 24.0 40.5 54-55 34.76084970965957 38.0 34.0 40.0 24.5 41.0 56-57 34.65768996575471 38.0 34.0 40.0 24.5 41.0 58-59 34.43881294876097 38.0 34.0 40.0 24.0 41.0 60-61 34.15312874314705 38.0 34.0 40.0 23.0 41.0 62-63 33.79882897137486 37.5 33.0 40.0 22.0 41.0 64-65 33.42645764674248 37.0 33.0 40.0 21.5 41.0 66-67 32.957266177617676 36.0 32.0 39.0 20.0 41.0 68-69 32.56514325905744 36.0 32.0 39.0 18.5 40.5 70-71 32.150827575785186 35.0 31.0 39.0 17.5 40.0 72-73 31.74558600321341 35.0 31.0 38.0 14.5 40.0 74-75 31.28953707757301 35.0 31.0 37.0 11.5 39.0 76-77 30.79835673760914 35.0 30.0 37.0 7.5 39.0 78-79 30.298157843858405 34.0 30.0 36.0 7.0 39.0 80-81 29.813121542971054 34.0 29.0 36.0 2.0 37.5 82-83 29.38018540453211 34.0 29.0 35.0 2.0 37.0 84-85 28.983515152592616 34.0 29.0 35.0 2.0 36.5 86-87 28.599821328965817 34.0 29.0 35.0 2.0 36.0 88-89 28.157431626106415 34.0 27.5 35.0 2.0 36.0 90-91 27.730911755620692 34.0 27.0 35.0 2.0 35.5 92-93 27.265224261037492 33.0 26.0 35.0 2.0 35.0 94-95 26.745079879730394 33.0 25.0 35.0 2.0 35.0 96-97 26.12313634333284 33.0 24.5 35.0 2.0 35.0 98-99 25.338334381507103 32.5 20.5 35.0 2.0 35.0 100-101 22.356327043549953 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40140151099276977 1101 2 0.2882308680586618 1101 3 0.3065871371751818 1101 4 0.33363023596123753 1101 5 0.34228653570351497 1101 6 0.4131043633668412 1101 7 0.5357925354159647 1101 8 0.43663637380831943 1101 9 0.4558967034966557 1101 10-11 0.572578395604971 1101 12-13 0.5661099464318724 1101 14-15 0.48138678418538206 1101 16-17 0.6146791310485824 1101 18-19 0.5273648014476606 1101 20-21 0.574523336158947 1101 22-23 0.46699442591273055 1101 24-25 0.6206405269232889 1101 26-27 0.4939338132806199 1101 28-29 0.6475191775713327 1101 30-31 0.5589926455441159 1101 32-33 0.5695482201478299 1101 34-35 0.3315932556363137 1101 36-37 0.2905032731964923 1101 38-39 0.3120178601215926 1101 40-41 0.1440356825754634 1101 42-43 0.12598270210542495 1101 44-45 -0.04217460253650529 1101 46-47 0.27867657654301325 1101 48-49 0.24803593319231254 1101 50-51 0.2325290941561704 1101 52-53 0.2796430720576666 1101 54-55 0.21316560781760785 1101 56-57 0.15975285932244532 1101 58-59 0.11061648542946756 1101 60-61 0.10181864248932726 1101 62-63 0.10507626238835144 1101 64-65 0.05468333948336124 1101 66-67 0.12017141611983817 1101 68-69 0.06287714811684708 1101 70-71 -0.14747769854235315 1101 72-73 -0.3078430734436317 1101 74-75 -0.1463998352831375 1101 76-77 -0.22307292443574056 1101 78-79 -0.1542959786833329 1101 80-81 -0.1081990339883312 1101 82-83 -0.11214787189974729 1101 84-85 -0.29038097377680927 1101 86-87 -0.2473849011434055 1101 88-89 -0.21993632336016233 1101 90-91 -0.07361501973284135 1101 92-93 -0.4381875587560131 1101 94-95 -0.2349080178902767 1101 96-97 -0.3100319429430698 1101 98-99 -0.21085505599458543 1101 100-101 0.03583628727666621 1102 1 9.996289743519071E-4 1102 2 0.20158130425197385 1102 3 0.24865559788259262 1102 4 0.10889091381162785 1102 5 0.07297913115049681 1102 6 0.3042126357316448 1102 7 0.25410913539555224 1102 8 0.24501536755487052 1102 9 0.2906936334277148 1102 10-11 0.26359411545590916 1102 12-13 0.31747975882827717 1102 14-15 0.24755233001646815 1102 16-17 0.19196505497252758 1102 18-19 0.17731007933394238 1102 20-21 0.12150587786916844 1102 22-23 0.13227421251408344 1102 24-25 0.07319311117951344 1102 26-27 -0.14143391580444842 1102 28-29 -0.19021867007244708 1102 30-31 -0.010295403920451918 1102 32-33 -0.127127279477385 1102 34-35 0.027529742793333867 1102 36-37 -0.0317881581298991 1102 38-39 -0.2258069094648789 1102 40-41 -0.08198600216181262 1102 42-43 -0.16738589267995252 1102 44-45 0.08684923237514397 1102 46-47 -0.09973405397538926 1102 48-49 0.1132738424204689 1102 50-51 0.13299959876092515 1102 52-53 0.367521508025753 1102 54-55 0.1777954153576431 1102 56-57 0.13516802596976163 1102 58-59 0.06963437305029885 1102 60-61 0.08763312220695241 1102 62-63 -0.06110969426328694 1102 64-65 0.1753799115212331 1102 66-67 0.0875475347559771 1102 68-69 0.2633673597207533 1102 70-71 0.21926533274602633 1102 72-73 -0.10231526018152337 1102 74-75 0.08759262678968938 1102 76-77 0.2172810273454573 1102 78-79 0.20031211596657172 1102 80-81 0.11713607297492601 1102 82-83 0.3546743392959364 1102 84-85 -0.0506407542549141 1102 86-87 0.052245592622494286 1102 88-89 0.288314222981203 1102 90-91 0.18184540263037974 1102 92-93 0.2183595638461533 1102 94-95 0.11996647315887188 1102 96-97 0.2911754626656773 1102 98-99 0.038243524749567825 1102 100-101 -0.0981164088871509 1103 1 0.32577977746556286 1103 2 0.15398301627527644 1103 3 0.2876655475512493 1103 4 0.3430865220544703 1103 5 0.36949515036211267 1103 6 0.3850413465079896 1103 7 0.4799332675567456 1103 8 0.4406258384355439 1103 9 0.08984059249199561 1103 10-11 0.40913057838843514 1103 12-13 0.15418213101882117 1103 14-15 0.36567540230065987 1103 16-17 0.5907457248242949 1103 18-19 0.5277698346806758 1103 20-21 0.42857573498517354 1103 22-23 0.2123468762134948 1103 24-25 0.4457356796749252 1103 26-27 0.2276169480424599 1103 28-29 0.48149425187126127 1103 30-31 0.4002173638431259 1103 32-33 0.3359560112138027 1103 34-35 0.6728649397554776 1103 36-37 0.7905987077430439 1103 38-39 0.6260884441133854 1103 40-41 0.6689688971792052 1103 42-43 0.6089641675141664 1103 44-45 0.8812846367675036 1103 46-47 1.0939220574917954 1103 48-49 1.0101491696633431 1103 50-51 1.1690399470178932 1103 52-53 1.2194276304474485 1103 54-55 1.2067001051518247 1103 56-57 1.0998105709222585 1103 58-59 0.8667879820610764 1103 60-61 1.0687772486940759 1103 62-63 1.1310228115277496 1103 64-65 1.1995541083326202 1103 66-67 1.0598860893692361 1103 68-69 1.1029457557869264 1103 70-71 1.0914904060298625 1103 72-73 0.7346752714057239 1103 74-75 0.8326402584982127 1103 76-77 0.9878396778088501 1103 78-79 0.751259514251128 1103 80-81 0.8471526272866612 1103 82-83 0.7045020749349433 1103 84-85 0.7596248499848883 1103 86-87 0.8424299032345601 1103 88-89 0.7835410259640945 1103 90-91 0.6775987649232391 1103 92-93 1.0519282025975016 1103 94-95 1.1238465988498447 1103 96-97 1.037025930219155 1103 98-99 0.9204583713455357 1103 100-101 1.5002031316619977 1104 1 0.34262269870417583 1104 2 0.4468109147860062 1104 3 0.09224579510081554 1104 4 0.5836889357322548 1104 5 0.5188810190874165 1104 6 0.6122302598460152 1104 7 0.665327238023167 1104 8 0.6500919886448315 1104 9 0.5437580557998913 1104 10-11 0.853066824194677 1104 12-13 0.8035708616833546 1104 14-15 0.6107506181522027 1104 16-17 0.42650393838547274 1104 18-19 0.5213762624326534 1104 20-21 0.4045195003323272 1104 22-23 0.5394485412897438 1104 24-25 0.5833446684536696 1104 26-27 0.25769920017604164 1104 28-29 0.5788439326014938 1104 30-31 0.5679347710570113 1104 32-33 0.493537247358951 1104 34-35 0.5646987641158177 1104 36-37 0.5121373353368739 1104 38-39 0.4334124736911136 1104 40-41 0.37130840984819713 1104 42-43 0.6441447599682917 1104 44-45 0.5647938158243164 1104 46-47 0.4628938935566467 1104 48-49 0.5671790327726995 1104 50-51 0.5959190349914465 1104 52-53 0.6184755859189437 1104 54-55 0.6329642850386037 1104 56-57 0.46132787298031985 1104 58-59 0.39581949364743707 1104 60-61 0.457899590054744 1104 62-63 0.6723508494558104 1104 64-65 0.7321949341410985 1104 66-67 0.7536581825180022 1104 68-69 0.7320870840701659 1104 70-71 0.6359678036603427 1104 72-73 0.4330677610230147 1104 74-75 0.4546451368780602 1104 76-77 0.601700703675057 1104 78-79 0.7031216963407871 1104 80-81 1.0332825249845996 1104 82-83 0.9325127736814984 1104 84-85 0.5840084904860703 1104 86-87 0.6807491435614068 1104 88-89 0.7718863917076639 1104 90-91 0.5451363605835553 1104 92-93 0.3700769040539029 1104 94-95 0.3057273344827074 1104 96-97 0.11667819642529409 1104 98-99 0.2632205679084265 1104 100-101 0.2830808851541775 1105 1 0.308506996224839 1105 2 0.3110606852175941 1105 3 0.4835772918868564 1105 4 0.4720268604338145 1105 5 0.15434842037299745 1105 6 0.34235330851451806 1105 7 0.30393880827109854 1105 8 0.4398881318955219 1105 9 0.44171948830678076 1105 10-11 0.5033294504572936 1105 12-13 0.3805515779367994 1105 14-15 0.2514852370686427 1105 16-17 0.20077758393184553 1105 18-19 0.4406306242324689 1105 20-21 0.38722657103939895 1105 22-23 -0.006670834284172145 1105 24-25 0.08707653254916892 1105 26-27 0.08862298346345909 1105 28-29 -0.13147379007985904 1105 30-31 -0.06020847963169018 1105 32-33 -0.16716064061166236 1105 34-35 0.14812208826641182 1105 36-37 0.10648076968031717 1105 38-39 -0.09026062089106546 1105 40-41 -0.24929765075786747 1105 42-43 -0.17097369733198065 1105 44-45 -0.008014264983764008 1105 46-47 -7.608355526329547E-4 1105 48-49 -0.13477138045044512 1105 50-51 -0.1442866583051483 1105 52-53 0.1044700762770745 1105 54-55 -0.07391174801924905 1105 56-57 -0.009349812970093296 1105 58-59 0.11221986095689118 1105 60-61 0.12324761576640242 1105 62-63 0.006055165341940949 1105 64-65 0.12668896536240482 1105 66-67 0.27737535423516846 1105 68-69 0.25619360381304546 1105 70-71 0.08861794140138812 1105 72-73 -0.15709201859131028 1105 74-75 -0.06382318268116549 1105 76-77 -0.1231404349842613 1105 78-79 -0.0903916186270699 1105 80-81 0.12528619807918062 1105 82-83 -0.00835040354531813 1105 84-85 -0.010819792342211088 1105 86-87 -0.29503047076365974 1105 88-89 -0.15349469185524 1105 90-91 -0.1887148790858646 1105 92-93 -0.09818755875601681 1105 94-95 -0.15526807414906685 1105 96-97 -0.12259453084743299 1105 98-99 0.16196069646673905 1105 100-101 -0.0073029532296615685 1106 1 0.36548311660429533 1106 2 0.36100594745726156 1106 3 0.2789932770575021 1106 4 0.24101606553685428 1106 5 0.2792927012583988 1106 6 0.2851887681154395 1106 7 0.42465934038319375 1106 8 0.524193878269962 1106 9 0.5639177218690961 1106 10-11 0.6003631436205268 1106 12-13 0.5068684463262869 1106 14-15 0.45902639942826085 1106 16-17 0.4740982686990449 1106 18-19 0.5082838790574655 1106 20-21 0.387286324610443 1106 22-23 0.4322056241173087 1106 24-25 0.20045609857250923 1106 26-27 0.2522949930589178 1106 28-29 0.23775573777969328 1106 30-31 0.30592084105307293 1106 32-33 0.5256754732254336 1106 34-35 0.4515271695007357 1106 36-37 0.2630220410792887 1106 38-39 0.39391994829440335 1106 40-41 0.30694049121137823 1106 42-43 0.502354838269369 1106 44-45 0.19334131237966545 1106 46-47 0.4255699898753491 1106 48-49 0.3981297208471233 1106 50-51 -0.07177809871513574 1106 52-53 0.08254531343769145 1106 54-55 0.14025595452137196 1106 56-57 0.16789934197483802 1106 58-59 0.32482044976945446 1106 60-61 0.2076227111758442 1106 62-63 0.053110448299086954 1106 64-65 0.29583562330591917 1106 66-67 0.41403526046131844 1106 68-69 0.4253215069860943 1106 70-71 0.4163571192445872 1106 72-73 0.005417631392333533 1106 74-75 0.12568374131839377 1106 76-77 0.048032610485151395 1106 78-79 -0.19075101236821368 1106 80-81 0.1324186809054808 1106 82-83 0.14058646266338215 1106 84-85 0.3224428834837596 1106 86-87 0.23097934277902965 1106 88-89 0.4465336583791988 1106 90-91 0.1867726359070474 1106 92-93 0.17339242161689938 1106 94-95 0.2555525560984542 1106 96-97 0.2797118697631902 1106 98-99 0.6580579595787199 1106 100-101 0.6794042760801204 1107 1 0.22236479139770182 1107 2 0.2772950839999382 1107 3 0.26297281265224015 1107 4 0.19198772149056254 1107 5 0.3269546391162024 1107 6 0.21352878144341503 1107 7 0.2853982559771211 1107 8 0.5168596474423808 1107 9 0.5569445372304571 1107 10-11 0.5329824184938232 1107 12-13 0.33973574840863563 1107 14-15 0.026075365357407065 1107 16-17 0.26840228495380813 1107 18-19 0.31382305737010796 1107 20-21 0.17554185697112246 1107 22-23 0.17975751970582365 1107 24-25 0.17061121400166002 1107 26-27 0.14478388800792175 1107 28-29 0.1248375819575287 1107 30-31 0.20617943078361378 1107 32-33 -0.01158334119875093 1107 34-35 0.09356283625510287 1107 36-37 0.21789846918020572 1107 38-39 0.021226658952379296 1107 40-41 0.17681454754611536 1107 42-43 0.32052967296591106 1107 44-45 0.038617176633977124 1107 46-47 0.03929569847650782 1107 48-49 0.06914990678591693 1107 50-51 0.1331962727668241 1107 52-53 0.13065663857322107 1107 54-55 0.22065402714926563 1107 56-57 0.060108330104490904 1107 58-59 0.2319067494109035 1107 60-61 0.2054233061338735 1107 62-63 0.32693709619647393 1107 64-65 0.35087596253569586 1107 66-67 0.2679498269466265 1107 68-69 0.3557408967028053 1107 70-71 0.4703474478158256 1107 72-73 0.3078447067837722 1107 74-75 0.43007592582067744 1107 76-77 0.2640702629935596 1107 78-79 0.27112740724811957 1107 80-81 0.3608869808580444 1107 82-83 0.483299955228329 1107 84-85 0.3815781202474824 1107 86-87 0.48085211240659476 1107 88-89 0.44361120073010696 1107 90-91 0.40944211156427457 1107 92-93 0.41465001462950113 1107 94-95 0.5372050721870956 1107 96-97 0.3038433904398019 1107 98-99 0.25573151621016166 1107 100-101 0.30200298284339766 1108 1 0.2705918953201767 1108 2 0.22311542889655556 1108 3 0.22288603695760756 1108 4 0.4450957539619935 1108 5 0.3118812433839011 1108 6 0.2091368854108353 1108 7 0.324386036292438 1108 8 0.21355735017434085 1108 9 0.1574188620019754 1108 10-11 0.33075603361665884 1108 12-13 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2316 88-89 -3.337198395558943 2316 90-91 -3.5756778420488295 2316 92-93 -4.09300237357083 2316 94-95 -3.9318606667416596 2316 96-97 -4.509853790106689 2316 98-99 -5.856705970199926 2316 100-101 -6.38345110137781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2139.0 3 882.0 4 764.0 5 906.0 6 976.0 7 1109.0 8 1417.0 9 1853.0 10 2329.0 11 3015.0 12 3718.0 13 4657.0 14 5026.0 15 6099.0 16 7191.0 17 9114.0 18 7510.0 19 5688.0 20 5769.0 21 6283.0 22 6860.0 23 8014.0 24 8846.0 25 10245.0 26 11851.0 27 13929.0 28 16397.0 29 19726.0 30 24119.0 31 29718.0 32 37170.0 33 48172.0 34 64043.0 35 88147.0 36 124712.0 37 164747.0 38 128964.0 39 17872.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72770915637346 18.88594400271152 14.06322059420024 22.323126246714786 2 15.05262350772267 20.20751586595146 40.793192003849384 23.946668622476476 3 19.392838845689226 25.471118337191484 29.852071564867472 25.283971252251824 4 12.20457389514582 16.488405226927703 38.442346205054 32.864674672872475 5 13.119963542998144 37.592615234136204 34.51222087607954 14.775200346786116 6 30.66539044747713 36.10988825300267 18.789659511835044 14.435061787685152 7 28.182397016646902 32.67153891371189 21.359288031140302 17.7867760385009 8 26.137236356442422 33.89074928841108 20.625909283387443 19.346105071759062 9 26.339383470664668 17.027418470981463 21.128128859143956 35.50506919920991 10-11 24.344547847506913 26.596584290046334 28.104963495211248 20.953904367235506 12-13 25.859290249150824 24.060571570841706 28.289777880528533 21.790360299478937 14-15 22.66170298843216 25.457523095663614 27.176193905767914 24.704580010136308 16-17 21.538016093061664 28.79100860338033 27.721379630756545 21.949595672801458 18-19 21.263249381223197 27.565270255701808 30.00070018749465 21.17078017558035 20-21 22.270687984684645 26.52699114330962 29.779113597212643 21.42320727479309 22-23 21.773278087978802 26.58789184371647 29.750367972066904 21.888462096237824 24-25 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27.261802708635308 28.618342651200283 22.19343125103128 50-51 21.77535491097224 27.417872667632654 28.770901300525363 22.035871120869743 52-53 22.04821747143015 27.54656264532162 28.423617704889253 21.981602178358976 54-55 21.569973948219793 27.317422246075708 28.680348712717592 22.43225509298691 56-57 21.903118992855315 27.7315910530351 28.100158894185363 22.26513105992422 58-59 22.19538064328301 27.247104081926327 28.27012752651455 22.287387748276117 60-61 21.873670057614632 27.310835662179358 28.915322603047965 21.90017167715805 62-63 21.96050434874399 27.299000802802308 28.78471286622647 21.955781982227236 64-65 21.697735312141134 27.56882316567395 28.55446767918246 22.17897384300246 66-67 21.638929436063048 27.627026255121027 28.25463538937604 22.47940891943988 68-69 21.6041049149932 27.537712405840566 28.576724549510367 22.281458129655864 70-71 21.70899588834668 27.46975736409504 28.324327569310846 22.496919178247428 72-73 21.740589624096163 27.261321261617983 28.461250805000198 22.53683830928566 74-75 21.542586003954664 27.785409976914448 28.23900713240124 22.432996886729644 76-77 21.741384743030242 27.43944965497849 28.35878458620481 22.460381015786464 78-79 21.639910169321936 27.482573070808364 28.34841628959276 22.52910047027694 80-81 21.679570974620447 27.80558188930189 28.05979443923948 22.45505269683819 82-83 21.77278202150265 27.698155827798704 28.268098916863565 22.260963233835078 84-85 21.55148798129417 27.69044921437633 28.16454060866178 22.59352219566772 86-87 21.722320113312872 27.669023276593546 28.14784684980543 22.46080976028815 88-89 21.77681955079406 27.39922872606439 27.993687556261875 22.830264166879672 90-91 21.593384850694505 27.240527237803903 28.388496629733044 22.777591281768544 92-93 21.822454679530853 27.370495877229345 28.38532318098513 22.421726262254673 94-95 21.45729799169652 27.179718268538355 28.413738075975925 22.949245663789196 96-97 21.23594238203381 27.124875044939365 28.502978639699894 23.136203933326925 98-99 21.26550954001381 27.358545102036956 28.116665758782116 23.25927959916712 100-101 21.307827920188004 27.287333968244436 28.212958746901194 23.191879364666363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 81.0 1 73.5 2 143.0 3 220.0 4 184.5 5 173.0 6 259.0 7 454.5 8 698.0 9 831.5 10 778.5 11 716.0 12 760.0 13 831.5 14 909.5 15 920.5 16 957.0 17 1083.5 18 1169.0 19 1250.0 20 1382.5 21 1621.5 22 1965.0 23 2341.0 24 2823.0 25 3516.0 26 4535.0 27 5789.0 28 7001.5 29 8556.0 30 10725.0 31 13050.0 32 15699.0 33 18129.0 34 20584.0 35 22723.5 36 25141.5 37 27778.5 38 28692.0 39 28954.5 40 29226.0 41 30114.5 42 31082.0 43 32018.0 44 33229.5 45 34318.0 46 34649.0 47 35867.0 48 41059.5 49 40456.5 50 34145.0 51 30798.0 52 28253.5 53 26738.5 54 25629.5 55 23609.5 56 21077.0 57 18640.0 58 16718.5 59 14537.0 60 12133.0 61 10001.5 62 8139.0 63 6167.0 64 4402.0 65 3352.0 66 2470.5 67 1885.5 68 1447.5 69 1007.5 70 753.0 71 579.5 72 481.5 73 439.5 74 330.0 75 247.5 76 186.0 77 117.0 78 77.0 79 45.5 80 28.0 81 22.0 82 12.0 83 4.5 84 5.5 85 5.5 86 4.0 87 2.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012555862476638317 2 0.010555813586554337 3 0.016778187911260053 4 0.019556033591932247 5 0.03200078224134367 6 0.04955676694319194 7 0.005666805188571276 8 0.025667294089411073 9 0.036556449157646075 10-11 0.041112116073948476 12-13 0.03133409927798235 14-15 0.02688954618890684 16-17 0.0303896317465538 18-19 0.024333928162688423 20-21 0.05427910460033468 22-23 0.01261141939025176 24-25 0.0027222887670587505 26-27 0.0012222520994957654 28-29 1.1111382722688777E-4 30-31 0.002277833458151199 32-33 0.001388922840336097 34-35 0.0 36-37 6.111260497478827E-4 38-39 5.5556913613443885E-5 40-41 0.0010000244450419898 42-43 0.0037223132121007403 44-45 0.0012778090131092094 46-47 0.0012222520994957654 48-49 5.000122225209949E-4 50-51 0.0037778701257141843 52-53 0.003833427039327628 54-55 0.004388996175462067 56-57 8.889106178151022E-4 58-59 0.0052779067932771685 60-61 0.003666756298487296 62-63 6.111260497478827E-4 64-65 9.444675314285461E-4 66-67 0.014944809762016403 68-69 0.04150101446924258 70-71 0.0512790312652087 72-73 0.014833695934789518 74-75 0.031222985450755462 76-77 0.027945127547562273 78-79 0.05644582423125899 80-81 0.08116865078924151 82-83 0.04605668138554498 84-85 0.016833744824873497 86-87 0.01872267988773059 88-89 0.018667122974117146 90-91 0.03844538422050316 92-93 0.024333928162688423 94-95 0.012666976303865205 96-97 0.01716708630655416 98-99 0.050112336079326386 100-101 0.07105729251159473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 899978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.10462481446706 #Duplication Level Percentage of deduplicated Percentage of total 1 86.47291752032143 59.75678521854169 2 8.587938217022334 11.869324968343033 3 2.087838638396268 4.328379173385656 4 0.8473112432122583 2.3421250225305106 5 0.4740164180830047 1.63783633637618 6 0.2805683109506846 1.1633140717845474 7 0.20053553813148034 0.9700553187180244 8 0.15148201896816052 0.8374486469546173 9 0.10754644522178361 0.668876107246289 >10 0.7118006184933331 9.239977537396022 >50 0.050958553273825 2.3759065625600666 >100 0.024990500599907133 2.9572225182277747 >500 0.0011286031752927232 0.6030986666417377 >1k 9.673741502509056E-4 1.2496498512938723 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2957 0.32856358710990713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2268 0.2520061601505814 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2002 0.2224498821082293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.164337350468567 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1452 0.16133727713344104 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1058 0.11755842920604725 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 963 0.10700261561949291 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 960 0.10666927413781227 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 916 0.1017802657398292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1111382722688777E-4 0.0 16-17 0.0 0.0 0.0 1.1111382722688777E-4 0.0 18-19 0.0 0.0 0.0 1.1111382722688777E-4 0.0 20-21 0.0 0.0 0.0 3.333414816806633E-4 0.0 22-23 0.0 0.0 0.0 5.555691361344389E-4 0.0 24-25 0.0 0.0 0.0 0.0011111382722688778 0.0 26-27 0.0 0.0 0.0 0.0018333781492436483 0.0 28-29 0.0 0.0 0.0 0.0030000733351259697 0.0 30-31 0.0 0.0 0.0 0.005666805188571276 0.0 32-33 0.0 0.0 0.0 0.00972245988235268 0.0 34-35 0.0 0.0 0.0 0.012500305563024873 0.0 36-37 0.0 0.0 0.0 0.017111529392940715 0.0 38-39 0.0 0.0 0.0 0.0254450664349573 0.0 40-41 0.0 0.0 0.0 0.035056412490083094 0.0 42-43 0.0 0.0 0.0 0.04538999842218365 0.0 44-45 0.0 0.0 0.0 0.04977899459764572 0.0 46-47 0.0 0.0 0.0 0.057001393367393426 0.0 48-49 0.0 0.0 0.0 0.06616828411361167 0.0 50-51 0.0 0.0 0.0 0.076724097700166 0.0 52-53 0.0 0.0 0.0 0.09272448882083784 0.0 54-55 0.0 0.0 0.0 0.10350253006184595 0.0 56-57 0.0 0.0 0.0 0.14044787761478614 0.0 58-59 0.0 0.0 0.0 0.19733815715495268 0.0 60-61 0.0 0.0 0.0 0.24289482631797665 0.0 62-63 0.0 0.0 0.0 0.26656207151730377 0.0 64-65 0.0 0.0 0.0 0.31761887512805864 0.0 66-67 0.0 0.0 0.0 0.41923247012704756 0.0 68-69 0.0 0.0 0.0 0.5580691972470437 0.0 70-71 1.1111382722688777E-4 0.0 0.0 0.6836833789270405 0.0 72-73 1.1111382722688777E-4 0.0 0.0 0.8279646835811543 0.0 74-75 1.1111382722688777E-4 0.0 0.0 1.0000800019556033 0.0 76-77 1.1111382722688777E-4 0.0 0.0 1.1038603165855165 0.0 78-79 1.1111382722688777E-4 0.0 0.0 1.2253077297445047 0.0 80-81 1.1111382722688777E-4 0.0 0.0 1.3625888632833247 0.0 82-83 1.1111382722688777E-4 0.0 0.0 1.5065923833693713 0.0 84-85 1.1111382722688777E-4 0.0 0.0 1.7026527315112148 0.0 86-87 1.1111382722688777E-4 0.0 0.0 2.0280495745451557 0.0 88-89 1.1111382722688777E-4 0.0 0.0 2.4672269766594295 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1120 0.0 39.446793 2 GTACCTG 1280 0.0 35.25626 1 GTACATG 4240 0.0 28.457087 1 ACCTGGG 1475 0.0 28.021933 3 TACATGG 4235 0.0 27.14618 2 ACATGGG 4255 0.0 25.903551 3 CATGGGG 1505 0.0 23.677942 4 CATGGGA 2630 0.0 23.124557 4 GTACACG 170 3.1459003E-6 22.354431 1 ATGGGAG 1140 0.0 22.08972 5 GTATAGG 240 5.9189915E-9 21.772285 1 GTATCAA 6690 0.0 20.804825 1 GAGTACT 3080 0.0 20.744658 12-13 GTACTAG 185 6.5457098E-6 20.541912 1 CCCGGAC 140 4.8447898E-4 20.360767 3 AAGACGG 165 6.306713E-5 20.157343 5 ATGGGGA 875 0.0 20.091524 5 GTGCTAT 245 1.753724E-7 19.38905 1 GTGTTAC 275 2.833076E-8 19.001268 1 GTACTTT 3465 0.0 18.580925 14-15 >>END_MODULE