FastQCFastQC Report
Thu 26 May 2016
SRR936531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899978
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.41967692543595514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.37100906911057824No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28480.31645217994217634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.23167232976806099No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12890.14322572329545832No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11110.12344746204907232No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.109891575127392No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9600.10666927413781227No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9560.10622481882890471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9200.043.374492
GTACCTG11400.035.8433721
CCCTACG650.0058354129.2389621
GTACACG1457.865128E-726.214241
TATCACG1151.2804638E-424.7854212
ACCTGGG15900.024.494193
GTACATG44950.024.100191
ACATGGG45300.023.0659223
GTAAGGT2903.6379788E-1222.9285374
ATCACGC1252.2570362E-422.7975223
TACATGG47150.022.5688382
GAGTACT41750.021.3886812-13
AGTACTT44950.021.02838512-13
ATGGGTA4400.020.5091325
GTATAGG3102.582965E-1019.9249381
GTACTTT45400.019.46148114-15
TAGGACC2702.2999302E-819.3497454
CCGTAAC757.7174854E-419.00215776-77
CATGGGA27500.018.9979344
CATATAG3003.7180143E-918.9979343
Produced by FastQC (version 0.11.5)