##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936531_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 899978 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14001120027378 31.0 31.0 34.0 31.0 34.0 2 32.2700521568305 33.0 31.0 34.0 31.0 34.0 3 32.39006953503308 34.0 31.0 34.0 31.0 34.0 4 36.064540466544734 37.0 35.0 37.0 35.0 37.0 5 35.96321465635827 37.0 35.0 37.0 35.0 37.0 6 36.01959936798455 37.0 35.0 37.0 35.0 37.0 7 35.96153794870541 37.0 35.0 37.0 35.0 37.0 8 35.93482285122525 37.0 35.0 37.0 35.0 37.0 9 37.595901233141255 39.0 37.0 39.0 35.0 39.0 10-11 37.49920553613533 39.0 37.0 39.0 35.0 39.0 12-13 37.39402852069718 39.0 37.0 39.0 34.0 39.0 14-15 38.548804526332866 40.0 38.0 41.0 34.0 41.0 16-17 38.53100298007285 40.0 38.0 41.0 34.0 41.0 18-19 38.678136021102745 40.0 38.0 41.0 34.0 41.0 20-21 38.688244601534706 40.0 38.0 41.0 34.0 41.0 22-23 38.597821835644865 40.0 38.0 41.0 34.0 41.0 24-25 38.49248592743378 40.0 38.0 41.0 34.0 41.0 26-27 38.30349519654925 40.0 38.0 41.0 34.0 41.0 28-29 38.31531715219705 40.0 38.0 41.0 34.0 41.0 30-31 38.19642146808033 40.0 38.0 41.0 34.0 41.0 32-33 38.00285562535973 40.0 37.0 41.0 33.5 41.0 34-35 37.818330559191445 40.0 37.0 41.0 33.0 41.0 36-37 37.64745415999058 40.0 37.0 41.0 33.0 41.0 38-39 37.45660338363827 40.0 37.0 41.0 33.0 41.0 40-41 37.24236759120778 40.0 36.0 41.0 32.0 41.0 42-43 36.98911473391572 39.0 36.0 41.0 31.0 41.0 44-45 36.69621868534564 39.0 36.0 41.0 31.0 41.0 46-47 36.28876761431946 39.0 35.5 40.5 30.0 41.0 48-49 35.88316547737834 39.0 35.0 40.0 29.0 41.0 50-51 35.599272982228456 39.0 35.0 40.0 28.0 41.0 52-53 35.35993213167433 38.5 35.0 40.0 27.5 41.0 54-55 35.05353464195791 38.0 34.0 40.0 27.0 41.0 56-57 34.730081179762166 38.0 34.0 40.0 26.0 41.0 58-59 34.38433550597904 38.0 34.0 40.0 26.0 41.0 60-61 34.02281389100622 37.0 33.0 40.0 25.0 41.0 62-63 33.717395869676814 37.0 33.0 39.0 24.0 41.0 64-65 33.40845442888604 36.5 33.0 39.0 23.0 40.0 66-67 32.97557329179158 36.0 32.0 39.0 22.0 40.0 68-69 32.53391971803755 35.5 31.5 38.5 21.5 40.0 70-71 32.032048005617916 35.0 31.0 38.0 19.5 40.0 72-73 31.47064706026147 35.0 31.0 37.0 18.0 39.0 74-75 30.945368664567354 34.0 30.0 36.5 17.0 39.0 76-77 29.564228792259364 33.0 28.5 35.0 14.0 37.5 78-79 30.319427252666173 34.0 30.0 36.0 13.5 38.0 80-81 30.137644475753852 34.0 30.0 35.5 9.5 37.0 82-83 29.74461042381036 34.0 29.5 35.0 7.0 37.0 84-85 29.30230238961397 34.0 29.0 35.0 4.5 36.0 86-87 28.88962952427726 34.0 29.0 35.0 2.0 36.0 88-89 28.397439715193038 33.0 28.5 35.0 2.0 35.5 90-91 27.8502407836636 33.0 27.0 35.0 2.0 35.0 92-93 27.3025335063746 33.0 26.0 34.0 2.0 35.0 94-95 26.596010124691936 32.0 25.0 34.0 2.0 35.0 96-97 25.81019980488412 32.0 24.5 34.0 2.0 35.0 98-99 24.71848145176882 31.5 16.5 34.0 2.0 35.0 100-101 20.941450235450198 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08656267090996295 1101 2 0.23309212896261755 1101 3 0.21478506226625882 1101 4 0.15187533374500362 1101 5 0.11639021662113436 1101 6 0.10786289795709791 1101 7 0.1676389918708523 1101 8 0.04279806702604105 1101 9 0.2320742109612226 1101 10-11 0.2617927009109522 1101 12-13 0.25239059340223946 1101 14-15 0.236397737862994 1101 16-17 0.24720600835978956 1101 18-19 0.1500941467221395 1101 20-21 -0.008786408991440453 1101 22-23 0.01129910186985228 1101 24-25 0.05161637895841409 1101 26-27 0.003922416182042809 1101 28-29 0.29278543769149934 1101 30-31 0.3143279927462288 1101 32-33 0.07195861188183983 1101 34-35 0.08138867498333724 1101 36-37 0.1867071011450463 1101 38-39 0.0029362283855576266 1101 40-41 0.03732761908734972 1101 42-43 0.23828896458619653 1101 44-45 0.29131652607367187 1101 46-47 0.11527824160188516 1101 48-49 -0.011489956096824017 1101 50-51 0.11229096541461558 1101 52-53 0.054514850314149044 1101 54-55 0.17967969455994393 1101 56-57 0.28551487550667076 1101 58-59 0.2534709048117634 1101 60-61 0.06957013761656583 1101 62-63 0.18872320224895134 1101 64-65 0.11546166426413862 1101 66-67 0.027919328458260395 1101 68-69 -0.09366584481632856 1101 70-71 -0.13337362315614598 1101 72-73 -0.05356164543933062 1101 74-75 -0.18800992590537646 1101 76-77 -0.20686657528612074 1101 78-79 -0.21092108670359622 1101 80-81 0.05941647284271667 1101 82-83 0.0887420125252838 1101 84-85 0.05447130766748387 1101 86-87 0.025012087135376504 1101 88-89 -0.22588456729569728 1101 90-91 -0.2145911500411053 1101 92-93 -0.43147880747111245 1101 94-95 -0.2902978215752121 1101 96-97 -0.03007814689801691 1101 98-99 -0.6454168296312126 1101 100-101 -0.21154407260226193 1102 1 0.14782047013083144 1102 2 0.33391947101629427 1102 3 0.2124518943309539 1102 4 0.10053930477571527 1102 5 -0.03256935939685235 1102 6 -8.599125425163834E-4 1102 7 0.10480744800342734 1102 8 -0.03338074583840722 1102 9 0.02016273761669396 1102 10-11 0.11697968866094044 1102 12-13 0.16669224421533357 1102 14-15 0.1958973912309503 1102 16-17 0.08526585429329003 1102 18-19 0.0904421160214568 1102 20-21 -0.01242340606706449 1102 22-23 0.01620614465867476 1102 24-25 -0.05192137930004748 1102 26-27 -0.05177941692078747 1102 28-29 0.23912996530417985 1102 30-31 0.17895287353648115 1102 32-33 0.11463024752757178 1102 34-35 0.07831579075843109 1102 36-37 0.11052723254850605 1102 38-39 0.19596487743557134 1102 40-41 0.33345132273630895 1102 42-43 0.1714808298184849 1102 44-45 0.23861367594891192 1102 46-47 0.18981433532275105 1102 48-49 0.1801926345289786 1102 50-51 0.18742847452030986 1102 52-53 0.24174154766743072 1102 54-55 0.2975497198623174 1102 56-57 0.1450616849083204 1102 58-59 0.19079877649104304 1102 60-61 0.2133119336983924 1102 62-63 0.2881175639357352 1102 64-65 0.2832065853129748 1102 66-67 0.25373760881144136 1102 68-69 0.3203409048308856 1102 70-71 -0.03108479596893332 1102 72-73 -0.21416911368818958 1102 74-75 -0.11967449870338598 1102 76-77 -0.07336397290649899 1102 78-79 0.061252630493928706 1102 80-81 0.26253475354780065 1102 82-83 0.2462181793731837 1102 84-85 0.005052488187111237 1102 86-87 -0.027559148387897636 1102 88-89 -0.08507031632002438 1102 90-91 0.10122301295688985 1102 92-93 0.1665448620425103 1102 94-95 0.278479253554206 1102 96-97 0.20635833498034017 1102 98-99 0.2683136701171698 1102 100-101 -0.2994869082282925 1103 1 0.19635898954111752 1103 2 0.24649354394884426 1103 3 0.2864962337441028 1103 4 0.1432388830083937 1103 5 0.19178501130535608 1103 6 0.12746830992248448 1103 7 0.11680393958850033 1103 8 0.15416943140190398 1103 9 0.20899032249189275 1103 10-11 0.2051274995778769 1103 12-13 0.24263635614595103 1103 14-15 0.30679048483744964 1103 16-17 0.2481160180911104 1103 18-19 0.2318529979006101 1103 20-21 0.21404574268186138 1103 22-23 0.23253178557195753 1103 24-25 0.2080080513549163 1103 26-27 0.29979844144589407 1103 28-29 0.4287503598597482 1103 30-31 0.3432375215475503 1103 32-33 0.30881626035957765 1103 34-35 0.3518652089809535 1103 36-37 0.3046983918033632 1103 38-39 0.23322730375254963 1103 40-41 0.345924242808465 1103 42-43 0.45450720160203417 1103 44-45 0.4666647306642915 1103 46-47 0.34903577022285504 1103 48-49 0.28075655058546545 1103 50-51 0.33194672473921116 1103 52-53 0.3993146068433404 1103 54-55 0.4366939458684058 1103 56-57 0.3129928861096758 1103 58-59 0.22796019848340165 1103 60-61 0.17035390322330812 1103 62-63 0.2335789420357699 1103 64-65 0.1566255148602238 1103 66-67 -0.06767152034560553 1103 68-69 0.22719449386359258 1103 70-71 -0.005142280797858945 1103 72-73 0.2344575617024809 1103 74-75 -0.06327509492597017 1103 76-77 -0.026884159289188858 1103 78-79 -0.02645662855765707 1103 80-81 0.13527153256445246 1103 82-83 0.07344731139697558 1103 84-85 0.2137192533825072 1103 86-87 0.2950820849937372 1103 88-89 -0.01426071467068013 1103 90-91 -0.04565547067112519 1103 92-93 -0.13666157965682757 1103 94-95 -0.148315262802182 1103 96-97 0.16345860479557217 1103 98-99 0.20047441255837128 1103 100-101 0.2556035240627921 1104 1 0.18068455720676013 1104 2 0.23206691712037042 1104 3 0.04166142078818069 1104 4 0.13488610893069364 1104 5 -0.00929238616158301 1104 6 0.07785046057986733 1104 7 0.1255402135235144 1104 8 0.05607932610707422 1104 9 0.07687666589025355 1104 10-11 0.13412360488742792 1104 12-13 0.16339825343176528 1104 14-15 0.2547469375700686 1104 16-17 0.21112831543514687 1104 18-19 0.17175145629659738 1104 20-21 0.180616248190276 1104 22-23 0.15377448864903442 1104 24-25 0.1544374550821388 1104 26-27 0.28488160531153994 1104 28-29 0.19151031593306556 1104 30-31 0.2916477960729438 1104 32-33 0.3059428733689771 1104 34-35 0.2697463600038503 1104 36-37 0.32520950144262173 1104 38-39 0.2918402281065866 1104 40-41 0.23541086814873324 1104 42-43 0.38559865527908954 1104 44-45 0.36656963251203933 1104 46-47 0.2234113564093434 1104 48-49 0.20204586238720879 1104 50-51 0.13068084813131264 1104 52-53 0.10491877448102116 1104 54-55 -0.032068396244540054 1104 56-57 0.14355790650712663 1104 58-59 0.057800739406268065 1104 60-61 -0.01210739722552745 1104 62-63 0.16539323979355913 1104 64-65 0.2207934632668227 1104 66-67 0.19625283614735167 1104 68-69 0.09360734740508292 1104 70-71 -0.11381788162111661 1104 72-73 0.09956455325648861 1104 74-75 0.18253441276324267 1104 76-77 0.05794494614220014 1104 78-79 0.019087863558521434 1104 80-81 -0.044396106343857156 1104 82-83 -0.1286354444379043 1104 84-85 -0.13729677593029166 1104 86-87 -0.030992143897620394 1104 88-89 -0.07484715055057123 1104 90-91 0.027605267529303035 1104 92-93 -0.1392655355110044 1104 94-95 -0.06364928790711488 1104 96-97 0.49302399070354497 1104 98-99 0.3385110568283203 1104 100-101 0.47149227476089806 1105 1 0.10695085656396941 1105 2 0.18258298690916774 1105 3 0.17515637028313336 1105 4 0.15626351939901184 1105 5 0.09201890860425976 1105 6 0.16103870667720344 1105 7 0.11139145319293675 1105 8 0.11938878432562205 1105 9 0.2102908073043963 1105 10-11 0.16616400892782934 1105 12-13 0.14205022772010523 1105 14-15 0.2876101147968839 1105 16-17 0.29615678191815675 1105 18-19 0.2018634856813506 1105 20-21 0.23474664304794146 1105 22-23 0.1751078219823725 1105 24-25 0.16637868556882296 1105 26-27 0.1885693922720506 1105 28-29 0.11418065569431946 1105 30-31 0.2979398070922272 1105 32-33 0.35324131230377986 1105 34-35 0.016567296642968188 1105 36-37 0.2604454977695241 1105 38-39 0.08988981488344905 1105 40-41 0.12855687365836843 1105 42-43 0.35074466078873456 1105 44-45 0.001566877691104196 1105 46-47 0.19869877606039665 1105 48-49 0.15075476964157275 1105 50-51 0.09747240001377122 1105 52-53 0.2345092244351079 1105 54-55 0.37862765517878216 1105 56-57 0.31213090926194553 1105 58-59 0.42525712140810157 1105 60-61 0.47682189570377176 1105 62-63 0.3662196860745581 1105 64-65 0.15263184710520505 1105 66-67 0.3499277672768315 1105 68-69 0.19080110314429533 1105 70-71 0.2686798226947644 1105 72-73 0.3208574825494175 1105 74-75 0.38589162121136056 1105 76-77 0.1660110618446815 1105 78-79 0.26817877264943135 1105 80-81 0.4566654179903935 1105 82-83 0.4165619843958872 1105 84-85 0.5071182837574959 1105 86-87 0.4344073051381905 1105 88-89 0.24699306983510283 1105 90-91 0.5512710159223246 1105 92-93 0.791199110953638 1105 94-95 0.8937134301828777 1105 96-97 0.7560653130175972 1105 98-99 0.8038039861212489 1105 100-101 0.7754151397746725 1106 1 0.03676701619760081 1106 2 0.2256570246367886 1106 3 0.07391724695961699 1106 4 0.10502374183931096 1106 5 0.10769964134878052 1106 6 0.05301471801730173 1106 7 0.06933627927514863 1106 8 0.06846565707668617 1106 9 0.03226965366793877 1106 10-11 0.17996970078258556 1106 12-13 0.12319426871890471 1106 14-15 0.2984996579219512 1106 16-17 0.18100641663629347 1106 18-19 0.17040693786758965 1106 20-21 0.09873399448180464 1106 22-23 0.08905962658382549 1106 24-25 0.12686761807345448 1106 26-27 0.17016093081746675 1106 28-29 0.19011217897422483 1106 30-31 0.1468845241350749 1106 32-33 0.1799001281362962 1106 34-35 0.15846763466499425 1106 36-37 0.23187631229356498 1106 38-39 0.39784270987853887 1106 40-41 0.19822168665587014 1106 42-43 0.24460400001019167 1106 44-45 0.41438249212792044 1106 46-47 0.5430634036909723 1106 48-49 0.5752650738613951 1106 50-51 0.51962876246062 1106 52-53 0.41660103557892825 1106 54-55 0.49889022196539656 1106 56-57 0.6397108896348627 1106 58-59 0.7035539265547612 1106 60-61 0.6263131180350356 1106 62-63 0.6305529103685217 1106 64-65 0.7112178246430929 1106 66-67 0.4940965602110836 1106 68-69 0.5858925871700222 1106 70-71 0.5713120329226626 1106 72-73 0.5956986121972214 1106 74-75 0.5802451268268847 1106 76-77 0.5237827334181162 1106 78-79 0.5298034591612613 1106 80-81 0.3683016626747282 1106 82-83 0.5923792344231451 1106 84-85 0.6412528383316953 1106 86-87 0.36964010417864657 1106 88-89 0.2307379177251967 1106 90-91 0.6322645825998983 1106 92-93 0.30215058843701215 1106 94-95 0.4000865628837822 1106 96-97 0.43137574808465473 1106 98-99 0.5662447232273458 1106 100-101 0.4332487457064147 1107 1 0.02952880829261062 1107 2 0.02576802713530668 1107 3 -0.03395778496800972 1107 4 -0.029424673686442304 1107 5 -0.0695049009195543 1107 6 -0.02147314372505349 1107 7 0.002808717815682371 1107 8 0.03232550970070491 1107 9 -0.031111236618194482 1107 10-11 0.011255985444464045 1107 12-13 -0.08119004190372436 1107 14-15 0.003155233300908833 1107 16-17 0.05480300935891336 1107 18-19 0.06089327476593809 1107 20-21 -0.10109114777614536 1107 22-23 -0.15357493513874232 1107 24-25 0.02828040333355375 1107 26-27 -0.0331757697196835 1107 28-29 0.01655986856668079 1107 30-31 0.16904613455471207 1107 32-33 0.05280034687650925 1107 34-35 0.215967601056974 1107 36-37 0.21577230794565594 1107 38-39 0.16557670333745733 1107 40-41 0.2485012093835337 1107 42-43 0.3708568590927115 1107 44-45 0.40244924342278665 1107 46-47 0.44445698436655334 1107 48-49 0.34360017081574057 1107 50-51 0.054620475682391145 1107 52-53 0.11751987892521498 1107 54-55 0.2690238282598898 1107 56-57 0.24398218127760884 1107 58-59 0.09984724969686454 1107 60-61 -0.03435531673371628 1107 62-63 -0.17801819172496636 1107 64-65 -0.19744469127270037 1107 66-67 -0.2766828002378432 1107 68-69 -0.05719933173285341 1107 70-71 -0.37257697226066 1107 72-73 -0.3691094667873962 1107 74-75 -0.3903632167969029 1107 76-77 -0.6294866550502931 1107 78-79 -0.5968810229583106 1107 80-81 -0.20640177052884923 1107 82-83 0.04560742905777104 1107 84-85 -0.20480561274174391 1107 86-87 -0.10258334500314348 1107 88-89 -0.0546037990494348 1107 90-91 -0.22754002995721478 1107 92-93 -0.07314444199264969 1107 94-95 0.013372486499466874 1107 96-97 0.33398833976253783 1107 98-99 0.16191140077660648 1107 100-101 0.19220096277967258 1108 1 -0.012208503213344102 1108 2 0.13709748470550664 1108 3 -0.0029285288122054 1108 4 -0.0011755898563805545 1108 5 0.13076351542402165 1108 6 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-2.227916199446753 2316 96-97 -1.6237865388342563 2316 98-99 -0.9818256435083832 2316 100-101 -0.6348070824475514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 7.0 5 18.0 6 44.0 7 102.0 8 237.0 9 544.0 10 884.0 11 1501.0 12 2413.0 13 3381.0 14 4062.0 15 5681.0 16 7308.0 17 10254.0 18 8832.0 19 5512.0 20 4799.0 21 5076.0 22 5323.0 23 6126.0 24 6943.0 25 8191.0 26 10009.0 27 12358.0 28 15387.0 29 19134.0 30 24226.0 31 30484.0 32 39131.0 33 51181.0 34 69817.0 35 97802.0 36 142005.0 37 188796.0 38 107864.0 39 4543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1383234318151 19.701465335795934 14.514447770880423 21.645763461508544 2 15.638723930089077 21.3586579663247 39.99242127909251 23.010196824493715 3 19.947907290979117 25.806114670631402 30.10509659597259 24.14088144241689 4 12.927552593010711 17.463446288071808 37.87163449732604 31.73736662159144 5 13.175758639809853 38.166396831764146 34.08453740445122 14.573307123974786 6 30.70375053612422 36.919235803541866 18.074441819688925 14.302571840644992 7 28.41278924414567 33.18592183116198 21.00213894830412 17.399149976388234 8 26.55381042596101 34.445561771846684 19.95166478696394 19.04896301522837 9 26.798766192062473 17.138418939129625 20.731062314856587 35.33175255395132 10-11 24.672825335730426 27.158608321536747 27.493172055316904 20.675394287415916 12-13 26.365811164272905 24.50859909908909 28.021407189953536 21.104182546684473 14-15 22.960821754790263 25.93195488993324 26.923147437738653 24.184075917537847 16-17 21.540126608581247 29.174810737172187 27.61748296273414 21.667579691512426 18-19 21.360826391824975 27.543986652747538 30.410168001817855 20.685018953609628 20-21 22.273729825828497 26.429956387677432 30.486902413955942 20.809411372538126 22-23 21.872786395614543 26.557863583912656 30.25230159298958 21.317048427483222 24-25 21.999746659347938 26.59693502256732 30.523381786584945 20.879936531499798 26-27 21.8822258791058 26.631569473442102 30.506835174676688 20.979369472775417 28-29 21.307921586928565 27.077399157201153 30.69265620529402 20.922023050576264 30-31 21.51905415097872 26.67470022761756 30.594311283264137 21.21193433813958 32-33 21.423154469990884 26.939173944373668 30.35463577759468 21.28303580804077 34-35 22.0511239263531 26.722279631543273 30.211784837272354 21.01481160483127 36-37 22.09297674248056 26.473859597074117 30.282943707935956 21.15021995250937 38-39 21.756404629223223 26.78037858289765 30.469978387327973 20.99323840055115 40-41 21.877534784183613 26.84726737764701 30.260406365488933 21.014791472680443 42-43 21.715286697808374 27.083842608436708 29.801038254523476 21.399832439231442 44-45 21.941823091304578 26.965227754458 29.547157965502773 21.545791188734647 46-47 22.33810159803906 27.411170050823465 28.879539277626787 21.371189073510685 48-49 22.39971687772129 27.53658616534319 28.568682431362568 21.49501452557295 50-51 22.114101454380197 27.743393953805178 28.75531544905336 21.38718914276127 52-53 22.447055503177587 27.911759820128434 28.335771362664875 21.305413314029103 54-55 21.979506142934763 27.755487084669944 28.48561685465295 21.779389917742343 56-57 22.35953301858224 27.930760686747842 28.175241939203065 21.534464355466852 58-59 22.72348468433632 27.489302314211393 28.116051695573475 21.67116130587881 60-61 22.223598833497878 27.74714972018061 28.659250374046273 21.37000107227524 62-63 22.27676676540982 27.532284122500773 28.81137094462309 21.379578167466313 64-65 22.070577489563277 27.84530644582403 28.46523184143066 21.61888422318203 66-67 22.11098437898449 27.824374828814403 28.23405355824366 21.830587233957445 68-69 22.061246826265464 27.774691237992588 28.38443717255671 21.779624763185236 70-71 22.26604625321203 27.83226612959233 28.15173275921939 21.74995485797625 72-73 22.311516413525506 27.49953053416878 28.286566388024344 21.902386664281373 74-75 22.114316669676217 28.189018376786635 27.930215437611988 21.76644951592516 76-77 22.27998331058261 27.78754660564251 28.048888214793465 21.883581868981405 78-79 22.26489272923452 27.7849450895025 27.99124143395128 21.9589207473117 80-81 22.3414622221679 28.118447618169274 27.75793119464331 21.78215896501952 82-83 22.30456566258166 28.188513451198222 27.7689934074628 21.737927478757317 84-85 22.074790644752493 28.06524250096254 27.796398861956373 22.063567992328593 86-87 22.177869187622782 28.02413619918753 27.683238219685858 22.114756393503825 88-89 22.24066749670402 28.0044824239313 27.410066786338717 22.344783293025962 90-91 22.112896657060165 27.792677391054564 27.785843385710706 22.30858256617456 92-93 22.19257902824894 27.90520276944719 27.785142278071433 22.11707592423244 94-95 21.973987069648008 27.774533862310697 27.74903316812513 22.502445899916165 96-97 21.941838513650904 27.69254103160414 27.856100767019605 22.50951968772535 98-99 21.87316314342065 28.040445567932444 27.344259562765632 22.742131725881272 100-101 21.54152064198266 28.55326113769248 27.238094497323225 22.667123723001637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 219.0 1 183.0 2 251.0 3 342.0 4 268.5 5 221.5 6 292.5 7 499.5 8 762.0 9 911.0 10 914.5 11 871.5 12 904.5 13 983.5 14 1052.5 15 1121.5 16 1231.5 17 1355.0 18 1496.0 19 1614.0 20 1778.5 21 2036.5 22 2288.5 23 2624.5 24 3085.5 25 3793.5 26 4833.0 27 6033.5 28 7337.0 29 8944.5 30 10971.5 31 13242.5 32 16024.0 33 18454.5 34 20525.0 35 22689.5 36 25172.0 37 27413.0 38 28500.0 39 28971.0 40 29279.0 41 30123.5 42 30798.5 43 31621.5 44 32562.5 45 33399.0 46 33982.5 47 35482.0 48 41165.5 49 40339.5 50 33732.0 51 30382.5 52 27703.0 53 26596.0 54 25353.5 55 23118.0 56 20665.0 57 18175.0 58 16660.0 59 14797.0 60 12244.0 61 9972.0 62 7934.0 63 6099.5 64 4339.0 65 3194.0 66 2412.0 67 1915.0 68 1452.0 69 987.5 70 758.5 71 568.5 72 481.0 73 416.0 74 308.5 75 277.0 76 219.5 77 129.5 78 78.0 79 51.5 80 37.5 81 24.5 82 18.5 83 12.0 84 5.0 85 0.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01288920395831898 2 0.0100002444504199 3 0.004889008397983062 4 1.1111382722688777E-4 5 0.00500012222520995 6 0.0 7 3.333414816806633E-4 8 0.0014444797539495409 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6667074084033166E-4 16-17 0.004333439261848622 18-19 0.001944491976470536 20-21 3.333414816806633E-4 22-23 3.888983952941072E-4 24-25 4.444553089075511E-4 26-27 1.1111382722688777E-4 28-29 5.5556913613443885E-5 30-31 3.888983952941072E-4 32-33 0.0029445164215125257 34-35 8.889106178151022E-4 36-37 0.0012222520994957654 38-39 0.0047778945707561745 40-41 0.0 42-43 6.666829633613266E-4 44-45 0.005611248274957833 46-47 0.0 48-49 0.0015000366675629849 50-51 7.777967905882144E-4 52-53 2.7778456806721945E-4 54-55 1.1111382722688777E-4 56-57 0.01261141939025176 58-59 0.0018889350628570922 60-61 0.002500061112604975 62-63 0.0 64-65 0.003444528644033521 66-67 5.000122225209949E-4 68-69 0.0014444797539495409 70-71 0.004500110002688955 72-73 0.0021111627173108676 74-75 0.007277955683361149 76-77 7.222398769747704E-4 78-79 0.006555715806386378 80-81 0.0021111627173108676 82-83 0.0023333903717646432 84-85 0.001388922840336097 86-87 2.2222765445377554E-4 88-89 0.002055605803697424 90-91 0.007389069510588037 92-93 0.0016667074084033167 94-95 2.7778456806721945E-4 96-97 1.1111382722688777E-4 98-99 3.333414816806633E-4 100-101 0.0017778212356302043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 899978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.098157411564 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06938448298442 56.22929566461165 2 9.328096270182758 12.331399512335262 3 2.409495677866123 4.777896737942344 4 0.9519110877003177 2.516782756665149 5 0.5388746478059053 1.7809310647887922 6 0.3212988711130068 1.2742358015391202 7 0.23230818128042752 1.0748599913987489 8 0.15487794654384018 0.8189717512187635 9 0.12089560992906703 0.7191879349912661 >10 0.7772574989876476 9.533724924884272 >50 0.06177275723752768 2.7904285753393188 >100 0.03062939435299353 3.5556375993766904 >500 0.0021878138004331844 0.9893580306549158 >1k 0.0010097602155845467 1.607289654253753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3777 0.41967692543595514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3339 0.37100906911057824 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2848 0.31645217994217634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.23167232976806099 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1289 0.14322572329545832 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1111 0.12344746204907232 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.109891575127392 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 960 0.10666927413781227 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 956 0.10622481882890471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 1.1111382722688777E-4 0.0 0.0 0.0 16-17 0.0 2.2222765445377554E-4 0.0 0.0 0.0 18-19 0.0 2.2222765445377554E-4 0.0 0.0 0.0 20-21 0.0 2.2222765445377554E-4 0.0 2.2222765445377554E-4 0.0 22-23 0.0 2.2222765445377554E-4 0.0 4.444553089075511E-4 0.0 24-25 0.0 2.7778456806721945E-4 0.0 0.0010000244450419898 0.0 26-27 0.0 3.333414816806633E-4 0.0 0.0017222643220167603 0.0 28-29 0.0 3.333414816806633E-4 0.0 0.0030000733351259697 0.0 30-31 0.0 3.333414816806633E-4 0.0 0.005944589756638496 0.0 32-33 0.0 3.333414816806633E-4 0.0 0.010055801364033343 0.0 34-35 0.0 3.333414816806633E-4 0.0 0.012889203958318982 0.0 36-37 0.0 3.333414816806633E-4 0.0 0.017444870874621377 0.0 38-39 0.0 3.333414816806633E-4 0.0 0.025667294089411076 0.0 40-41 0.0 3.333414816806633E-4 0.0 0.035334197058150316 0.0 42-43 0.0 3.333414816806633E-4 0.0 0.04600112447193154 0.0 44-45 0.0 3.333414816806633E-4 0.0 0.05039012064739361 0.0 46-47 0.0 3.333414816806633E-4 0.0 0.0572236210218472 0.0 48-49 0.0 3.333414816806633E-4 0.0 0.06622384102722512 0.0 50-51 0.0 3.333414816806633E-4 0.0 0.07705743918184667 0.0 52-53 0.0 3.333414816806633E-4 0.0 0.09233559042554373 0.0 54-55 0.0 3.333414816806633E-4 0.0 0.1032803024073922 0.0 56-57 0.0 3.333414816806633E-4 0.0 0.14044787761478614 0.0 58-59 0.0 3.333414816806633E-4 0.0 0.19750482789579302 0.0 60-61 0.0 3.333414816806633E-4 0.0 0.24300594014520355 0.0 62-63 0.0 3.333414816806633E-4 0.0 0.26628428694923656 0.0 64-65 0.0 3.333414816806633E-4 0.0 0.3180633304369662 0.0 66-67 0.0 3.333414816806633E-4 0.0 0.41951025469511477 0.0 68-69 0.0 3.333414816806633E-4 0.0 0.5594025631737665 0.0 70-71 0.0 3.333414816806633E-4 0.0 0.686127883126032 0.0 72-73 0.0 3.333414816806633E-4 0.0 0.8312425414843474 0.0 74-75 0.0 3.333414816806633E-4 0.0 1.0043023273902252 0.0 76-77 0.0 3.333414816806633E-4 0.0 1.108693768069886 0.0 78-79 0.0 3.333414816806633E-4 0.0 1.2293633844382863 0.0 80-81 0.0 3.333414816806633E-4 0.0 1.3657000504456775 0.0 82-83 0.0 3.333414816806633E-4 0.0 1.5090368875683629 1.1111382722688777E-4 84-85 0.0 3.333414816806633E-4 0.0 1.7029860729928954 1.1111382722688777E-4 86-87 0.0 3.333414816806633E-4 0.0 2.026605094791206 1.1111382722688777E-4 88-89 0.0 3.333414816806633E-4 0.0 2.4662825091280007 1.1111382722688777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 920 0.0 43.37449 2 GTACCTG 1140 0.0 35.843372 1 CCCTACG 65 0.00583541 29.238962 1 GTACACG 145 7.865128E-7 26.21424 1 TATCACG 115 1.2804638E-4 24.785421 2 ACCTGGG 1590 0.0 24.49419 3 GTACATG 4495 0.0 24.10019 1 ACATGGG 4530 0.0 23.065922 3 GTAAGGT 290 3.6379788E-12 22.928537 4 ATCACGC 125 2.2570362E-4 22.797522 3 TACATGG 4715 0.0 22.568838 2 GAGTACT 4175 0.0 21.38868 12-13 AGTACTT 4495 0.0 21.028385 12-13 ATGGGTA 440 0.0 20.509132 5 GTATAGG 310 2.582965E-10 19.924938 1 GTACTTT 4540 0.0 19.461481 14-15 TAGGACC 270 2.2999302E-8 19.349745 4 CCGTAAC 75 7.7174854E-4 19.002157 76-77 CATGGGA 2750 0.0 18.997934 4 CATATAG 300 3.7180143E-9 18.997934 3 >>END_MODULE