FastQCFastQC Report
Thu 26 May 2016
SRR936530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004236
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.3294046419367559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.25472100183622176No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22060.21966948008237105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.16061961530954874No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14190.14130144707021058No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11740.11690479130403611No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10550.10505498707475136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11900.039.1353952
GTACCTG12450.036.266851
GTACACG1701.07320375E-1030.753841
GTACATG48700.028.4000821
TACATGG48950.027.9595642
ACATGGG48400.026.9026973
ACCTGGG18700.023.6337153
CATGGGG16950.022.1497924
CATGGGA33650.021.3257434
GTATCAA81250.021.0003551
GTATAGG3503.6379788E-1220.3694231
GAGTACT34150.020.2491212-13
AGTACTT37950.020.1000512-13
CATGGGT6200.019.9293884
AGGCGAC1759.885508E-519.012415
CTAAGGG3054.5347406E-918.6970042
GCGACAG1801.2279038E-418.4824477
TCAACGC92300.018.4328964
CAACGCA92500.018.3957925
ATCAACG92800.018.2814583
Produced by FastQC (version 0.11.5)