##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936530_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1004236 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.322355502093135 31.0 31.0 34.0 30.0 34.0 2 31.38148403363353 31.0 31.0 34.0 28.0 34.0 3 31.421111173070873 31.0 31.0 34.0 28.0 34.0 4 35.06491501997539 37.0 35.0 37.0 32.0 37.0 5 34.997813263017854 37.0 35.0 37.0 32.0 37.0 6 35.10357625100076 37.0 35.0 37.0 32.0 37.0 7 34.95155720368519 37.0 35.0 37.0 32.0 37.0 8 34.935031207803746 37.0 35.0 37.0 32.0 37.0 9 36.44263698971158 39.0 37.0 39.0 32.0 39.0 10-11 36.29520003266165 39.0 36.0 39.0 32.0 39.0 12-13 36.2128334375585 39.0 35.0 39.0 32.0 39.0 14-15 37.28156379576116 40.0 37.0 41.0 32.0 41.0 16-17 37.215328369028796 39.0 37.0 41.0 31.5 41.0 18-19 37.17616974496035 39.5 36.5 41.0 31.0 41.0 20-21 37.07548872974081 39.0 36.0 41.0 31.0 41.0 22-23 36.96019810084482 39.0 36.0 41.0 31.0 41.0 24-25 36.81337255386184 39.0 36.0 41.0 30.5 41.0 26-27 36.605424920038715 39.0 36.0 40.0 30.0 41.0 28-29 36.72158884963295 39.0 36.0 41.0 30.0 41.0 30-31 36.66498263356422 39.0 36.0 41.0 30.0 41.0 32-33 36.51598379265431 39.0 35.5 41.0 30.0 41.0 34-35 36.33496907101518 39.0 35.0 40.0 30.0 41.0 36-37 36.095626924348466 39.0 35.0 40.0 29.5 41.0 38-39 35.90953769830996 38.0 35.0 40.0 29.0 41.0 40-41 35.767070688563244 38.0 35.0 40.0 28.0 41.0 42-43 35.50965410521033 38.0 35.0 40.0 27.0 41.0 44-45 35.25256911721946 38.0 34.5 40.0 26.5 41.0 46-47 34.927233239995374 38.0 34.0 40.0 24.5 41.0 48-49 34.68384075058054 38.0 34.0 40.0 24.0 41.0 50-51 34.02323059519874 37.5 33.5 39.5 23.5 40.5 52-53 34.23043288629366 38.0 33.5 39.5 24.0 40.5 54-55 34.59411731903656 38.0 34.0 40.0 24.0 41.0 56-57 34.520317435343884 38.0 34.0 40.0 23.5 41.0 58-59 34.30161386367348 38.0 34.0 40.0 23.0 41.0 60-61 33.99291351833632 38.0 33.0 40.0 22.5 41.0 62-63 33.68073739638889 37.0 33.0 40.0 22.0 41.0 64-65 33.36990259261767 37.0 33.0 40.0 21.5 41.0 66-67 33.00278669555762 36.0 32.0 39.0 20.5 41.0 68-69 32.614530847330705 36.0 32.0 39.0 19.5 41.0 70-71 32.21019312193548 35.0 31.0 39.0 18.0 40.0 72-73 31.786292763852323 35.0 31.0 38.0 16.5 40.0 74-75 31.33625910642518 35.0 31.0 37.0 13.0 39.0 76-77 30.903156230208836 35.0 30.5 37.0 9.0 39.0 78-79 30.439958336486644 34.5 30.0 36.0 7.0 39.0 80-81 30.010480604160776 34.0 29.5 36.0 6.0 37.5 82-83 29.583048705682728 34.0 29.0 35.0 2.0 37.0 84-85 29.17647246264822 34.0 29.0 35.0 2.0 36.5 86-87 28.81423041994113 34.0 29.0 35.0 2.0 36.0 88-89 28.413825535033595 34.0 28.5 35.0 2.0 36.0 90-91 28.043576410325862 34.0 27.5 35.0 2.0 35.5 92-93 27.65340417989397 34.0 27.0 35.0 2.0 35.0 94-95 27.22452590825264 33.0 26.0 35.0 2.0 35.0 96-97 26.63249773957516 33.0 25.0 35.0 2.0 35.0 98-99 25.9423048964586 33.0 24.5 35.0 2.0 35.0 100-101 23.830435276170142 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07358843517137359 1101 2 0.15502878156340927 1101 3 0.17697485149197334 1101 4 0.07223850988611957 1101 5 0.20735955437551468 1101 6 0.054853293213120935 1101 7 0.025932881497716664 1101 8 -0.02601698713473155 1101 9 0.31782584761565147 1101 10-11 0.15261362422799607 1101 12-13 0.20948117218413387 1101 14-15 0.22387105562287957 1101 16-17 0.42537392274358865 1101 18-19 0.42781990426514227 1101 20-21 0.37971248507822253 1101 22-23 0.2644338542974509 1101 24-25 0.07724675330142361 1101 26-27 0.12852069041678504 1101 28-29 0.23791207068730102 1101 30-31 0.05332907684177002 1101 32-33 -0.02093214939778676 1101 34-35 0.03852936834416454 1101 36-37 0.1548174339493258 1101 38-39 -0.06430990275433857 1101 40-41 -0.0511487385053897 1101 42-43 -0.07650135953879555 1101 44-45 -0.3044473589975638 1101 46-47 -0.05723006555592036 1101 48-49 -0.07983712639290985 1101 50-51 -0.21506221465843112 1101 52-53 -0.11313445717232895 1101 54-55 -0.06597927639316481 1101 56-57 -0.19744425685137657 1101 58-59 -0.06661718584372522 1101 60-61 -0.021748392115924275 1101 62-63 -0.07092037324192546 1101 64-65 0.052458909007718546 1101 66-67 -0.09434975970581405 1101 68-69 -0.11985796249722114 1101 70-71 -0.059602983092567285 1101 72-73 -0.2115199150859297 1101 74-75 -0.2457575435554311 1101 76-77 -0.38886804181589696 1101 78-79 0.01425875882197758 1101 80-81 -0.14240716375058682 1101 82-83 -0.1570755048427941 1101 84-85 -0.0019553313922244797 1101 86-87 0.039313240236793945 1101 88-89 -0.31214656245810346 1101 90-91 -0.1873166767888037 1101 92-93 -0.5449616045254224 1101 94-95 -0.7862344910299406 1101 96-97 -0.6888874691262146 1101 98-99 -0.8119893640245941 1101 100-101 -0.5455309114199736 1102 1 0.1320526399492401 1102 2 0.23953221966498006 1102 3 0.10803899255462213 1102 4 0.33200726112601586 1102 5 0.39725287338907833 1102 6 0.36821326286762 1102 7 0.3697572549593602 1102 8 0.24771289265287777 1102 9 0.5466177592725003 1102 10-11 0.41734582968566514 1102 12-13 0.37528290154178734 1102 14-15 0.20459203272739757 1102 16-17 0.13275920142658038 1102 18-19 0.47050263802277215 1102 20-21 0.18046705160472953 1102 22-23 -0.14083951694190944 1102 24-25 -0.06134967670780611 1102 26-27 0.1851296579782442 1102 28-29 0.10708909107681563 1102 30-31 0.053854759696363885 1102 32-33 0.05239495457757215 1102 34-35 -0.025326720562475202 1102 36-37 -0.28992641601362124 1102 38-39 -0.2599970416502728 1102 40-41 -0.5073112472048464 1102 42-43 -0.25620218234393377 1102 44-45 -0.27576872601854063 1102 46-47 0.044675510559528675 1102 48-49 0.015009695374430976 1102 50-51 -0.1471932302053247 1102 52-53 -0.2850204728452397 1102 54-55 -0.2679209750305276 1102 56-57 -0.02069139370533435 1102 58-59 -0.06903623165341344 1102 60-61 -0.5892080095273684 1102 62-63 -0.3392868016550352 1102 64-65 0.05145277722577646 1102 66-67 -0.2785151711309979 1102 68-69 -0.3432037093125899 1102 70-71 -0.12841961832165794 1102 72-73 -0.2542410976817244 1102 74-75 -0.09014281771503718 1102 76-77 -0.09649499891022373 1102 78-79 -0.16935189079537238 1102 80-81 -0.020198339386819697 1102 82-83 -0.1031411547800154 1102 84-85 -0.3460313993490942 1102 86-87 -0.3430879187856597 1102 88-89 -0.6496973133089874 1102 90-91 -0.8789007753832578 1102 92-93 -0.6994403125927633 1102 94-95 -0.7013397794576157 1102 96-97 -0.7535157657909863 1102 98-99 -0.8951852185290186 1102 100-101 -0.566852569230484 1103 1 -0.021206910366174725 1103 2 0.24135533420756872 1103 3 0.13344657977089014 1103 4 -0.009714523945355324 1103 5 0.20067204184486087 1103 6 -0.1633336306981903 1103 7 0.04698566188872633 1103 8 -0.28381105261798467 1103 9 0.14377637788714281 1103 10-11 0.1600227737887181 1103 12-13 0.2879420097200551 1103 14-15 0.3062077201818241 1103 16-17 0.41903726846619804 1103 18-19 0.3004538601469662 1103 20-21 0.09969032831527613 1103 22-23 -0.040515945983230495 1103 24-25 0.15970551139172073 1103 26-27 0.13950621654993967 1103 28-29 0.138567777210433 1103 30-31 -0.06166600086112339 1103 32-33 0.00999574858857244 1103 34-35 0.17412997855478096 1103 36-37 0.32199528695385027 1103 38-39 0.2293982962761163 1103 40-41 0.19875583576089184 1103 42-43 0.4737703306983434 1103 44-45 0.3135807790632299 1103 46-47 0.43510760145517224 1103 48-49 0.5327729505645635 1103 50-51 0.035898147355325705 1103 52-53 0.30022893687361574 1103 54-55 0.39406625806805806 1103 56-57 0.29833548384766573 1103 58-59 0.3544924001692422 1103 60-61 0.46639889637952336 1103 62-63 0.25167322751480015 1103 64-65 0.222414068551835 1103 66-67 0.23316742335223495 1103 68-69 0.1630002984796306 1103 70-71 0.33827252520207907 1103 72-73 0.10634641691594382 1103 74-75 -0.005895483541966229 1103 76-77 -0.25738262620951247 1103 78-79 -0.13463494526442688 1103 80-81 0.22951640055800127 1103 82-83 0.17120379888992687 1103 84-85 -0.21264539117949965 1103 86-87 0.08035433820464633 1103 88-89 -0.020483751376637827 1103 90-91 0.14489628774495245 1103 92-93 -0.07979810200901127 1103 94-95 -0.20294870991408587 1103 96-97 -0.1521241429385647 1103 98-99 -0.09518980818800671 1103 100-101 0.07180804078619474 1104 1 0.20203674971892482 1104 2 0.11321488860432183 1104 3 0.17212378173971743 1104 4 0.17045787657405498 1104 5 0.25476918458468845 1104 6 0.19496394625075197 1104 7 0.19054797294874248 1104 8 0.12255490969923244 1104 9 0.33777122999428855 1104 10-11 0.3756838135296263 1104 12-13 0.3298818748380725 1104 14-15 0.2881612249062826 1104 16-17 0.013613119048997646 1104 18-19 0.09708835462183174 1104 20-21 0.11164519315100563 1104 22-23 0.13571726434129516 1104 24-25 0.24967109801028187 1104 26-27 0.06787679041425321 1104 28-29 0.037525624025981585 1104 30-31 0.06890628520363862 1104 32-33 0.17747003478678636 1104 34-35 0.12994657139905996 1104 36-37 0.20655231410988506 1104 38-39 0.018701662679823983 1104 40-41 -0.31363817820976436 1104 42-43 0.17596498571766972 1104 44-45 -0.06209125704900487 1104 46-47 0.13935012212629516 1104 48-49 0.1486018229040127 1104 50-51 -0.07762976315449066 1104 52-53 0.030671242493184536 1104 54-55 0.2866838691994147 1104 56-57 0.30985055798655736 1104 58-59 0.39727944780392477 1104 60-61 0.21616344315452096 1104 62-63 0.0011683182829784755 1104 64-65 -0.06087818343316087 1104 66-67 -0.12545735017641135 1104 68-69 0.07813416679005769 1104 70-71 -0.003672600723696462 1104 72-73 -0.12567926352463132 1104 74-75 -0.44924460357563234 1104 76-77 -0.28642260578218526 1104 78-79 -0.21762354599221467 1104 80-81 -0.18074095559098424 1104 82-83 -0.3168073334925232 1104 84-85 -0.08676613056678306 1104 86-87 0.027609928775227388 1104 88-89 -0.09097023495008472 1104 90-91 -0.188974781405161 1104 92-93 -0.4503514363346284 1104 94-95 -0.3923531864765444 1104 96-97 -0.13993029628726106 1104 98-99 0.02517234068515606 1104 100-101 0.19465752522702928 1105 1 0.10305532833242737 1105 2 0.20085197621218143 1105 3 0.18296191522911798 1105 4 0.36442891155435575 1105 5 0.4589180340419148 1105 6 0.32327442639035553 1105 7 0.47957239478763114 1105 8 0.14883015909587982 1105 9 0.30152011479253105 1105 10-11 0.30612445357406415 1105 12-13 0.073104802921101 1105 14-15 0.2208262897125408 1105 16-17 0.04238715110702174 1105 18-19 0.054111817204528734 1105 20-21 -0.21449227942150628 1105 22-23 -0.331539150020177 1105 24-25 -0.35409863267389596 1105 26-27 -0.06284943428155287 1105 28-29 -0.1484568130826176 1105 30-31 -0.28369244212115063 1105 32-33 -0.313448585877218 1105 34-35 -0.3187119646192187 1105 36-37 -0.13383781883744206 1105 38-39 -0.35963190205303164 1105 40-41 -0.2793085647621112 1105 42-43 0.12956661406169445 1105 44-45 0.1939607261334544 1105 46-47 0.006720331907125399 1105 48-49 0.022878810122122673 1105 50-51 -0.11835796083566663 1105 52-53 -0.06286022197348018 1105 54-55 0.03541023074200922 1105 56-57 0.18038983401385877 1105 58-59 0.24455808980540894 1105 60-61 0.5180441565913512 1105 62-63 0.002857977647380494 1105 64-65 0.3166529010511354 1105 66-67 -0.04252885528524786 1105 68-69 -0.03758840090758753 1105 70-71 0.30492722403268857 1105 72-73 0.3751517818321126 1105 74-75 0.17003531654835413 1105 76-77 0.2741556848684539 1105 78-79 0.191057788968223 1105 80-81 0.3031320479238566 1105 82-83 0.14420527966349894 1105 84-85 0.14788195062189757 1105 86-87 0.14591631015396445 1105 88-89 5.181599764867428E-4 1105 90-91 -0.00986391357724159 1105 92-93 -0.17014366835239514 1105 94-95 -0.1654489068254641 1105 96-97 -0.06261052717305304 1105 98-99 0.09285005717712025 1105 100-101 -0.018292699075495022 1106 1 -0.0027673114121924414 1106 2 0.057038013056082804 1106 3 0.22395591689477357 1106 4 0.3929943813162069 1106 5 0.410384332559822 1106 6 0.48073667171394874 1106 7 0.4677231915928246 1106 8 0.5095230221875084 1106 9 0.36770051985916297 1106 10-11 0.5438160007117503 1106 12-13 0.5118139321584536 1106 14-15 0.434064196489075 1106 16-17 0.2987492336567925 1106 18-19 0.3779734187359267 1106 20-21 0.4255592071008536 1106 22-23 0.33744225668823447 1106 24-25 0.22796661448528965 1106 26-27 0.3418257132892748 1106 28-29 0.2345784761112455 1106 30-31 0.19769214647119782 1106 32-33 0.13873046250691345 1106 34-35 0.09467558451839153 1106 36-37 0.22479438959168618 1106 38-39 0.18882982222583422 1106 40-41 -0.00889547881499908 1106 42-43 0.009870982832154596 1106 44-45 -0.15246906414253658 1106 46-47 -0.06772446052466563 1106 48-49 -0.16949975204722278 1106 50-51 -0.14180356924316584 1106 52-53 -0.054185037887400256 1106 54-55 0.07197956550411533 1106 56-57 -0.11138643696310879 1106 58-59 0.0039867523215022516 1106 60-61 -0.231781693687644 1106 62-63 -0.18784298902401275 1106 64-65 -0.2591524360760431 1106 66-67 -0.23306620548488155 1106 68-69 -0.25296490839110675 1106 70-71 -0.26536290378478 1106 72-73 0.06665889317978824 1106 74-75 -0.2109263269755921 1106 76-77 -0.39132166092126397 1106 78-79 -0.18131785197999406 1106 80-81 -0.14956418299867735 1106 82-83 0.11603691339854905 1106 84-85 0.35207495626350394 1106 86-87 0.15688036930307447 1106 88-89 0.18244949442681246 1106 90-91 0.18374422988842198 1106 92-93 0.2228283370799531 1106 94-95 0.2559565516447435 1106 96-97 0.08597183792060292 1106 98-99 0.48671212217147897 1106 100-101 0.5569817622892472 1107 1 0.5720852183710079 1107 2 0.48971533508712284 1107 3 0.6197054027829196 1107 4 0.5121771738110539 1107 5 0.3828026480764919 1107 6 0.5320635605971731 1107 7 0.6644187609190197 1107 8 0.6944905679576792 1107 9 0.806708916202652 1107 10-11 0.832050435718017 1107 12-13 0.9342276407624013 1107 14-15 0.9585563088663847 1107 16-17 0.9370012141391726 1107 18-19 0.9790314403511715 1107 20-21 0.9628301112123765 1107 22-23 0.9363536530140735 1107 24-25 1.107116511662916 1107 26-27 0.959279933319344 1107 28-29 1.0638296273493282 1107 30-31 0.9620823774637728 1107 32-33 0.885091491553986 1107 34-35 0.7559993759879617 1107 36-37 0.918853726627539 1107 38-39 0.9653675998903495 1107 40-41 1.1020595635979973 1107 42-43 1.110511441719872 1107 44-45 0.8649132096335421 1107 46-47 1.0545508446587988 1107 48-49 0.9309152621753967 1107 50-51 0.6941099619456352 1107 52-53 1.055874107030398 1107 54-55 1.1271527364127394 1107 56-57 1.09682494387566 1107 58-59 1.0735339810184499 1107 60-61 1.2279769236896314 1107 62-63 1.3238066189963291 1107 64-65 0.9989449481777299 1107 66-67 1.0021245305037283 1107 68-69 0.9560649346013932 1107 70-71 1.0488241329509336 1107 72-73 1.2381624914726892 1107 74-75 1.0293594584442154 1107 76-77 0.9305388260784184 1107 78-79 1.0576407504983294 1107 80-81 1.1215325366834286 1107 82-83 1.001189029735837 1107 84-85 1.1698367612323075 1107 86-87 1.3573854633723101 1107 88-89 1.2479602834243266 1107 90-91 1.2881447137122777 1107 92-93 1.1816489086115851 1107 94-95 1.4298307496008427 1107 96-97 1.657680394819895 1107 98-99 1.6462730343794085 1107 100-101 1.8898405182659808 1108 1 0.2174788418733442 1108 2 0.2025563287918537 1108 3 0.1599341985589966 1108 4 0.3317940424227075 1108 5 0.4394736018434955 1108 6 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0.938838929234791 2311 60-61 0.7050770387644292 2311 62-63 0.626568414163188 2311 64-65 0.7729955810891198 2311 66-67 0.6603523786049905 2311 68-69 0.9412202855795258 2311 70-71 0.8717815552754473 2311 72-73 0.8779208343955851 2311 74-75 0.6474837392267183 2311 76-77 0.7253604601405641 2311 78-79 0.5730607735132907 2311 80-81 0.5189433537144943 2311 82-83 0.4584387420488376 2311 84-85 0.7583868187697043 2311 86-87 1.0565224023826687 2311 88-89 1.1562456689249032 2311 90-91 0.9566142188906426 2311 92-93 0.7903945933066368 2311 94-95 0.2652162501762163 2311 96-97 0.6384053660511952 2311 98-99 0.571531952676299 2311 100-101 0.7806345343764534 2312 1 0.17881470333956173 2312 2 0.2275137406158123 2312 3 0.3289359317125964 2312 4 0.12123365609240011 2312 5 0.3771126614965681 2312 6 0.39517555292126616 2312 7 0.583724550679591 2312 8 0.5963630038775918 2312 9 0.4847943393177019 2312 10-11 0.5863641468324587 2312 12-13 0.5739343434709596 2312 14-15 0.5189681873907475 2312 16-17 0.5697619892524131 2312 18-19 0.737979223168999 2312 20-21 0.7801074287263745 2312 22-23 0.4795848003512617 2312 24-25 0.4384363016593795 2312 26-27 0.31130760864903095 2312 28-29 0.4306703937921199 2312 30-31 0.3249425440512681 2312 32-33 0.3715566551291474 2312 34-35 0.5890811492236665 2312 36-37 0.49315267884121283 2312 38-39 0.4402113244886223 2312 40-41 0.15517885568078782 2312 42-43 0.2291428804098672 2312 44-45 0.06089340146738209 2312 46-47 -0.004431364968596085 2312 48-49 -0.3971809990093291 2312 50-51 0.06731410973865337 2312 52-53 0.19240190827294867 2312 54-55 -0.24265256085319464 2312 56-57 -0.06851857511804837 2312 58-59 0.06448498975584727 2312 60-61 -0.4521637480794283 2312 62-63 -0.40567675572354744 2312 64-65 0.014189171327210204 2312 66-67 0.24674215384255405 2312 68-69 0.19963222148190596 2312 70-71 0.08841257319333096 2312 72-73 0.2769811100013193 2312 74-75 0.1588042623867203 2312 76-77 0.28586573732419396 2312 78-79 0.205388344606245 2312 80-81 0.34848330953342455 2312 82-83 0.5541170617655204 2312 84-85 0.07446732650139509 2312 86-87 0.2988766223763548 2312 88-89 0.24770925460462578 2312 90-91 -0.052735688188850105 2312 92-93 0.35732365814060785 2312 94-95 0.5921842364309917 2312 96-97 0.6502571140086957 2312 98-99 0.912776386211835 2312 100-101 1.021864942174762 2313 1 0.2569706194456174 2313 2 0.13933276185311883 2313 3 0.22077078199620104 2313 4 0.3356512790972843 2313 5 0.42328071320478955 2313 6 0.4571726509294507 2313 7 0.5330056481601346 2313 8 0.6954264519715139 2313 9 0.5197501498967796 2313 10-11 0.4078916498003977 2313 12-13 0.5334053895071023 2313 14-15 0.649755682444173 2313 16-17 0.5192739269914952 2313 18-19 0.310397109991321 2313 20-21 0.3240330322087672 2313 22-23 0.2554389093078555 2313 24-25 0.23572557748050826 2313 26-27 0.47705091428153423 2313 28-29 0.42381114039546475 2313 30-31 0.5209918779122376 2313 32-33 0.5600542148178462 2313 34-35 0.5442321847071057 2313 36-37 0.5789922778387009 2313 38-39 0.45689777746316196 2313 40-41 0.6236526718000377 2313 42-43 0.6026753959091451 2313 44-45 0.7078397146186788 2313 46-47 0.876290175725245 2313 48-49 0.8770572281374811 2313 50-51 0.7810721891477002 2313 52-53 0.8808335136020631 2313 54-55 0.5171416615449047 2313 56-57 0.6516700543503475 2313 58-59 0.7029231905209201 2313 60-61 0.9346255936960617 2313 62-63 0.948333269339102 2313 64-65 0.9229083376641043 2313 66-67 1.040107145268486 2313 68-69 0.7862632727943932 2313 70-71 0.479785742959848 2313 72-73 0.7675166594011351 2313 74-75 0.7135793580205423 2313 76-77 0.8790262702354212 2313 78-79 0.8643713283814698 2313 80-81 1.1491692348730886 2313 82-83 0.953009028524523 2313 84-85 0.7871041817975275 2313 86-87 0.8889373003871732 2313 88-89 1.1815240547629138 2313 90-91 1.17856625086932 2313 92-93 1.217665301723251 2313 94-95 1.1252603477619552 2313 96-97 1.2911686032581358 2313 98-99 1.4764223432095953 2313 100-101 1.625479241079919 2314 1 -0.5637030439995314 2314 2 -0.5540130883881353 2314 3 -0.45739471229187956 2314 4 -0.8974891092729536 2314 5 -0.8116737615897947 2314 6 -0.367821364604211 2314 7 -0.36550068672195124 2314 8 -0.25372577266372076 2314 9 -0.3026121424855006 2314 10-11 -0.3428632581830229 2314 12-13 -0.21076660549767468 2314 14-15 -0.546622057071481 2314 16-17 -0.686704194376361 2314 18-19 -0.9467453145395126 2314 20-21 -0.7972389336667973 2314 22-23 -1.1407464620607186 2314 24-25 -0.774356445759409 2314 26-27 -0.4060825522963043 2314 28-29 -0.5253533415804768 2314 30-31 -0.6794596248377331 2314 32-33 -0.6258406143148321 2314 34-35 -0.6352549393169866 2314 36-37 -0.6565202664679717 2314 38-39 -0.470190512882958 2314 40-41 -0.45145021775988425 2314 42-43 -0.6886830346897952 2314 44-45 -0.18629507575195703 2314 46-47 -0.5110417905540885 2314 48-49 -0.38208819006856487 2314 50-51 -0.19771972735240695 2314 52-53 -0.44470126696270285 2314 54-55 -0.6559312681902512 2314 56-57 -0.7863741355259464 2314 58-59 -0.6531633722611829 2314 60-61 -0.7070058150905751 2314 62-63 -0.7947204235813388 2314 64-65 -0.6972575834678381 2314 66-67 -0.6388420978046696 2314 68-69 -0.7125196453755969 2314 70-71 -0.4258594113528602 2314 72-73 -0.33375925766425496 2314 74-75 -0.20596850473185313 2314 76-77 -0.08096865378348994 2314 78-79 -0.004499640755984302 2314 80-81 0.06572125935316464 2314 82-83 0.16747211374044113 2314 84-85 0.1761658751563573 2314 86-87 0.20606210318819151 2314 88-89 0.16050343647381027 2314 90-91 0.42738522004139057 2314 92-93 0.3128341790373632 2314 94-95 0.445365847414422 2314 96-97 0.46608085511908826 2314 98-99 0.4713593404783687 2314 100-101 0.48477722712674165 2315 1 -0.8952693368216913 2315 2 -1.0390019306588556 2315 3 -1.237762320724844 2315 4 -0.9206659143631271 2315 5 -0.7986836135835773 2315 6 -0.9004078318627506 2315 7 -1.3810584154553993 2315 8 -0.6464517835457642 2315 9 -1.7268033614142766 2315 10-11 -1.0828106721546575 2315 12-13 -1.3560187070871805 2315 14-15 -1.3796092973473861 2315 16-17 -1.747413351395167 2315 18-19 -1.6734070069757152 2315 20-21 -1.32433774574924 2315 22-23 -0.9757835150486187 2315 24-25 -1.1935784633127753 2315 26-27 -1.3663351133620623 2315 28-29 -1.7043810623999534 2315 30-31 -1.4398331584569917 2315 32-33 -1.3474185792053603 2315 34-35 -1.2740448846050256 2315 36-37 -1.4746191046102624 2315 38-39 -1.7168373649220072 2315 40-41 -1.5554646686309042 2315 42-43 -1.4604666666369681 2315 44-45 -2.1563955158890593 2315 46-47 -2.359616934123274 2315 48-49 -2.3826347610727296 2315 50-51 -2.3142689646904095 2315 52-53 -2.4350351210357886 2315 54-55 -2.118414003541531 2315 56-57 -2.2422273829257904 2315 58-59 -2.1901505247565467 2315 60-61 -2.503533271882663 2315 62-63 -2.541615529953525 2315 64-65 -2.3393571293407156 2315 66-67 -2.2279564881894096 2315 68-69 -2.4305212391409867 2315 70-71 -1.9325718793438256 2315 72-73 -1.8463825104543723 2315 74-75 -1.688560526852548 2315 76-77 -1.8661710496435404 2315 78-79 -1.6589378384569606 2315 80-81 -1.6090971103702323 2315 82-83 -1.5713307243140697 2315 84-85 -1.6115615827329854 2315 86-87 -1.637966968628632 2315 88-89 -1.5075750338405953 2315 90-91 -1.783810638911742 2315 92-93 -1.8253474745559473 2315 94-95 -2.1561269703545527 2315 96-97 -2.0287805544991855 2315 98-99 -1.7291944998515731 2315 100-101 -1.8879265917381218 2316 1 -3.3585034878174262 2316 2 -2.5500825923892663 2316 3 -2.550505234131073 2316 4 -2.0453043067984 2316 5 -5.3672922665592 2316 6 -5.529504399753527 2316 7 -4.74728266853954 2316 8 -3.682423543862061 2316 9 -7.306064386444071 2316 10-11 -6.537193249147734 2316 12-13 -6.2673048259291875 2316 14-15 -4.901342331131971 2316 16-17 -4.384225230108413 2316 18-19 -4.417065728691064 2316 20-21 -4.597346503587254 2316 22-23 -4.21034832091604 2316 24-25 -3.5101736975613136 2316 26-27 -4.068154811029181 2316 28-29 -3.4887067271634535 2316 30-31 -3.2643890936983695 2316 32-33 -2.2090520546922505 2316 34-35 -2.313183092616633 2316 36-37 -2.089597354810685 2316 38-39 -2.319060660832072 2316 40-41 -1.832495691435419 2316 42-43 -1.8635893708671318 2316 44-45 -2.0836114196107616 2316 46-47 -2.0024972660249105 2316 48-49 -2.508021517319513 2316 50-51 -2.339121197131295 2316 52-53 -2.2346389911893283 2316 54-55 -2.1272442392685136 2316 56-57 -1.9069451537466193 2316 58-59 -2.1759171327937636 2316 60-61 -2.4754597218947048 2316 62-63 -2.071345082550849 2316 64-65 -2.1971199940347077 2316 66-67 -3.1037707720311545 2316 68-69 -2.245920767869926 2316 70-71 -2.2064631272976385 2316 72-73 -2.1385720918888538 2316 74-75 -2.245411789926049 2316 76-77 -2.8368202514396117 2316 78-79 -3.096332861761404 2316 80-81 -3.272514770536855 2316 82-83 -3.3083989671680314 2316 84-85 -3.4294858867241587 2316 86-87 -3.34215673905927 2316 88-89 -3.4616215329916358 2316 90-91 -3.8766565247397757 2316 92-93 -4.124518482545607 2316 94-95 -4.494777598766774 2316 96-97 -4.379376044807039 2316 98-99 -4.6894687271773705 2316 100-101 -5.1978642839731215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2554.0 3 1015.0 4 948.0 5 1072.0 6 1134.0 7 1359.0 8 1655.0 9 2165.0 10 2601.0 11 3465.0 12 4323.0 13 5294.0 14 5599.0 15 6652.0 16 7807.0 17 9474.0 18 8576.0 19 6198.0 20 6593.0 21 7051.0 22 7739.0 23 8811.0 24 9989.0 25 11581.0 26 13692.0 27 16270.0 28 19108.0 29 22886.0 30 27767.0 31 34006.0 32 42865.0 33 54757.0 34 71969.0 35 97256.0 36 134721.0 37 177826.0 38 145147.0 39 22306.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.780000617486216 18.790503858790863 14.002794623089404 22.42670090063352 2 15.09937718716452 20.320787448937118 40.701525861571035 23.878309502327333 3 19.385097701784733 25.509621790869087 29.800015336705414 25.305265170640766 4 12.290880111570454 16.507944414006076 38.26069631917119 32.94047915525228 5 13.253199300612215 37.55473307197617 34.44116225896278 14.750905368448844 6 30.603193582940985 36.07582891164088 18.81702912089281 14.503948384525323 7 28.35510319141836 32.621616856474326 21.26592895186924 17.75735100023807 8 26.229709041323485 33.821468449345936 20.583550889131704 19.36527162019887 9 26.527624372671326 16.914045887741658 21.119774833949002 35.438554905638014 10-11 24.4051447105515 26.64772362150242 28.01171254061804 20.93541912732805 12-13 25.885477687715692 24.154970550494546 28.219321107205765 21.740230654584 14-15 22.70346586056379 25.517438863753505 27.00467874990974 24.774416525772967 16-17 21.604527247201226 28.859806437571812 27.586704921633263 21.948961393593706 18-19 21.34233837127353 27.5746414545527 29.868186764885333 21.21483340928844 20-21 22.312553444635963 26.530502475448465 29.671416028236198 21.485528051679374 22-23 21.790954397510927 26.605917129118744 29.58345450706526 22.01967396630507 24-25 21.891122956949683 26.653236366967835 29.770901526294143 21.68473914978834 26-27 21.70466676226439 26.67644551726336 29.883350859582876 21.735536860889376 28-29 21.3688874018265 27.036039023438164 29.9924717466444 21.60260182809094 30-31 21.479619959637766 26.80294356507178 29.79834318654512 21.91909328874533 32-33 21.301027711515836 26.895659971430607 29.70868478717096 22.0946275298826 34-35 22.054473476008837 26.756362068424693 29.469928456448752 21.719235999117718 36-37 22.044229871046834 26.435897359293264 29.63731983751642 21.882552932143486 38-39 21.687783042002874 26.833788524210593 29.716603222104105 21.76182521168243 40-41 21.868425966266262 26.802614053650338 29.712130453725027 21.616829526358377 42-43 21.584231078882944 27.006303844194825 29.25454714738727 22.15491792953496 44-45 21.740379507151545 27.046967134308247 29.0080843557236 22.204569002816605 46-47 22.061764096681166 27.228223634944154 28.618274301738282 22.091737966636394 48-49 22.070198918178267 27.33661146030001 28.396077798755666 22.197111822766054 50-51 21.88102770616311 27.50824771936743 28.52754932449859 22.083175249970875 52-53 22.054586291040916 27.592878698182133 28.28470469560403 22.06783031517292 54-55 21.699477747897973 27.39441062289355 28.508372514273677 22.397739114934797 56-57 22.044049661051126 27.711852381106496 28.04749018504642 22.19660777279596 58-59 22.25870199395644 27.25004095260999 28.17908016205782 22.312176891375753 60-61 21.97209101315089 27.323612106208895 28.697353241816977 22.006943638823238 62-63 21.928245695533395 27.364114483053243 28.723131469894252 21.984508351519118 64-65 21.819508066122285 27.48635729934276 28.53774148575981 22.156393148775145 66-67 21.655317733612424 27.626403347520327 28.22164974118856 22.496629177678685 68-69 21.66945258976324 27.553949562592646 28.431552682826517 22.345045164817602 70-71 21.823126013329137 27.50080667970633 28.19579610323907 22.480271203725465 72-73 21.81103358033199 27.357449643443616 28.313295604711865 22.518221171512533 74-75 21.584626943776556 27.863763986900995 28.15982099156691 22.391788077755542 76-77 21.79847908555928 27.508804285208555 28.225445467205933 22.467271162026233 78-79 21.75172112881744 27.551011259683992 28.27393019055693 22.42333742094164 80-81 21.74342639323411 27.89252144222427 27.97349939291438 22.390552771627238 82-83 21.84047747912373 27.732238056424553 28.133108210045805 22.29417625440591 84-85 21.69393051552675 27.663129968191626 27.990027267168454 22.652912249113168 86-87 21.781944041256764 27.757691700596677 28.045995097348452 22.41436916079811 88-89 21.903817274331487 27.49235190784049 27.857179845683806 22.746650972144213 90-91 21.71076809081285 27.377906496150235 28.223002588421124 22.688322824615796 92-93 21.841331051970066 27.50015437047719 28.22723931806095 22.43127525949179 94-95 21.52986804374011 27.32098568104537 28.24185624082254 22.90729003439198 96-97 21.4570272413994 27.213665561794425 28.40895907491472 22.920348121891458 98-99 21.32591479235954 27.483064749878235 28.011715477837505 23.17930497992472 100-101 21.313913771606785 27.354324644874374 28.22972918448501 23.10203239903384 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 110.0 1 95.5 2 167.5 3 230.0 4 185.0 5 189.0 6 292.5 7 493.0 8 746.0 9 884.5 10 837.0 11 805.5 12 815.5 13 872.5 14 1033.5 15 1105.0 16 1116.5 17 1195.5 18 1310.5 19 1407.5 20 1516.5 21 1769.5 22 2089.0 23 2584.5 24 3173.0 25 3883.5 26 5004.5 27 6387.5 28 7751.5 29 9547.5 30 11809.0 31 14371.0 32 17481.0 33 20083.5 34 22660.5 35 25157.0 36 27998.0 37 30792.0 38 31812.0 39 32276.5 40 32576.5 41 33465.0 42 34397.5 43 35517.5 44 37049.0 45 38280.0 46 38661.0 47 39789.5 48 45467.5 49 44686.5 50 37798.5 51 34172.0 52 31404.0 53 30055.0 54 28941.5 55 26732.5 56 24011.5 57 21131.0 58 18819.5 59 16676.5 60 14011.0 61 11509.0 62 9308.5 63 7073.0 64 5057.5 65 3788.5 66 2911.5 67 2218.0 68 1659.5 69 1225.0 70 895.0 71 673.0 72 551.0 73 467.5 74 366.0 75 280.0 76 220.0 77 144.0 78 91.0 79 52.5 80 26.5 81 22.5 82 19.5 83 14.0 84 8.5 85 5.0 86 3.0 87 3.0 88 3.0 89 2.5 90 2.5 91 2.0 92 2.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01643040082211751 2 0.008165411317658399 3 0.010854022361277629 4 0.0384371801050749 5 0.0498886715871568 6 0.06582118147527075 7 0.032362910710231466 8 0.0277823141173987 9 0.017625339063726056 10-11 0.052328337163774254 12-13 0.0481958424115447 14-15 0.02275361568396273 16-17 0.026935899529592643 18-19 0.03520088903405176 20-21 0.02753336865039692 22-23 0.016729135382519647 24-25 0.003883549285227775 26-27 0.002788189230419941 28-29 0.0018919855492135315 30-31 0.007916465850656619 32-33 0.0014438837086103266 34-35 0.0022405092030160245 36-37 0.007269207636451989 38-39 0.007816887663855908 40-41 0.004829542059834541 42-43 7.468364010053414E-4 44-45 0.0012447273350089023 46-47 0.002788189230419941 48-49 3.983127472028487E-4 50-51 0.0018919855492135315 52-53 9.459927746067658E-4 54-55 0.002738400137019585 56-57 0.0028379783238202975 58-59 0.0026388219502188727 60-61 7.468364010053414E-4 62-63 0.001742618269012463 64-65 0.0035848147248256387 66-67 0.0029873456040213657 68-69 0.00801604403745733 70-71 0.011949382416085463 72-73 0.004680174779633472 74-75 0.0037341820050267064 76-77 0.0037341820050267064 78-79 0.03873591466547704 80-81 0.025790750381384456 82-83 0.004530807499432405 84-85 0.009758662306469794 86-87 0.008513934971460892 88-89 0.006373003955245579 90-91 0.014837149833306116 92-93 0.015733353514512525 94-95 0.007717309477055193 96-97 0.004729963873033829 98-99 0.026388219502188726 100-101 0.04695111507653579 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1004236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.46838899528117 #Duplication Level Percentage of deduplicated Percentage of total 1 86.13412488203672 58.97464768191416 2 8.823773934352994 12.082991722874066 3 2.125585350406918 4.3660621384299585 4 0.8745672676504194 2.3952084753611635 5 0.47553778091859905 1.627965288294372 6 0.2950283650584217 1.2120070118077104 7 0.2069580418005393 0.9919058598190691 8 0.14813257414302733 0.8113918967437701 9 0.10671979105524806 0.6576238950719268 >10 0.7281463675841334 9.388065190332954 >50 0.052978705770659065 2.4909961517950276 >100 0.026404595541132586 3.1431140230755292 >500 0.0010211718406224629 0.5262827051471461 >1k 0.0010211718406224629 1.331737959333251 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3308 0.3294046419367559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 0.25472100183622176 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2206 0.21966948008237105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 0.16061961530954874 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1419 0.14130144707021058 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1174 0.11690479130403611 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1055 0.10505498707475136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.9789093400356094E-5 0.0 22-23 0.0 0.0 0.0 2.987345604021365E-4 0.0 24-25 0.0 0.0 0.0 4.481018406032048E-4 0.0 26-27 0.0 0.0 0.0 8.464145878060535E-4 0.0 28-29 0.0 0.0 0.0 0.0021907201096156683 0.0 30-31 0.0 0.0 0.0 0.004182283845629912 0.0 32-33 0.0 0.0 0.0 0.007318996729852346 0.0 34-35 0.0 0.0 0.0 0.009310560465866589 0.0 36-37 0.0 0.0 0.0 0.013094531564293653 0.0 38-39 0.0 0.0 0.0 0.020562895574347068 0.0 40-41 0.0 0.0 0.0 0.030271768787416505 0.0 42-43 0.0 0.0 0.0 0.03888528194567811 0.0 44-45 0.0 0.0 0.0 0.04301777669790766 0.0 46-47 0.0 0.0 0.0 0.04968951521355538 0.0 48-49 0.0 0.0 0.0 0.05830302837181699 0.0 50-51 0.0 0.0 0.0 0.06686675243667822 0.0 52-53 0.0 0.0 0.0 0.08021022946797365 0.0 54-55 0.0 0.0 0.0 0.09156214276325486 0.0 56-57 0.0 0.0 0.0 0.11984234781465711 0.0 58-59 0.0 9.957818680071219E-5 0.0 0.16619599377038863 0.0 60-61 0.0 9.957818680071219E-5 0.0 0.20458338478206317 0.0 62-63 0.0 9.957818680071219E-5 0.0 0.22639100769141915 0.0 64-65 0.0 9.957818680071219E-5 0.0 0.2719978172461453 0.0 66-67 0.0 9.957818680071219E-5 0.0 0.3636595381962009 0.0 68-69 0.0 9.957818680071219E-5 0.0 0.4816596895550448 0.0 70-71 0.0 9.957818680071219E-5 0.0 0.5879594039648051 0.0 72-73 0.0 9.957818680071219E-5 0.0 0.7082498536200654 0.0 74-75 0.0 9.957818680071219E-5 0.0 0.8527875917612991 0.0 76-77 0.0 9.957818680071219E-5 0.0 0.9433041635631465 0.0 78-79 0.0 9.957818680071219E-5 0.0 1.042782772177058 0.0 80-81 0.0 9.957818680071219E-5 0.0 1.1621272290577114 0.0 82-83 0.0 9.957818680071219E-5 0.0 1.2914792937118367 0.0 84-85 0.0 9.957818680071219E-5 0.0 1.451053338059978 0.0 86-87 0.0 9.957818680071219E-5 0.0 1.7405769161830487 0.0 88-89 0.0 9.957818680071219E-5 0.0 2.1425740562975237 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1190 0.0 39.135395 2 GTACCTG 1245 0.0 36.26685 1 GTACACG 170 1.07320375E-10 30.75384 1 GTACATG 4870 0.0 28.400082 1 TACATGG 4895 0.0 27.959564 2 ACATGGG 4840 0.0 26.902697 3 ACCTGGG 1870 0.0 23.633715 3 CATGGGG 1695 0.0 22.149792 4 CATGGGA 3365 0.0 21.325743 4 GTATCAA 8125 0.0 21.000355 1 GTATAGG 350 3.6379788E-12 20.369423 1 GAGTACT 3415 0.0 20.24912 12-13 AGTACTT 3795 0.0 20.10005 12-13 CATGGGT 620 0.0 19.929388 4 AGGCGAC 175 9.885508E-5 19.01241 5 CTAAGGG 305 4.5347406E-9 18.697004 2 GCGACAG 180 1.2279038E-4 18.482447 7 TCAACGC 9230 0.0 18.432896 4 CAACGCA 9250 0.0 18.395792 5 ATCAACG 9280 0.0 18.281458 3 >>END_MODULE