FastQCFastQC Report
Thu 26 May 2016
SRR936530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004236
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.41444441346456407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.36355996000940016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27950.2783210321079906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.21339605431392622No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12730.1267630317973066No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12310.1225807479516767No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.10983474004118553No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10870.10824148905237414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG11800.038.6510962
GTACCTG12550.037.5605431
GTACACG1553.092282E-1134.1358341
TATCACG953.486075E-530.0054592
TGTACGG1004.9614933E-528.492415
ACCTGGG16950.027.1851273
ACACGGG2551.8189894E-1124.2176783
GTACATG44950.023.8629821
GTACGGG1050.002199431222.6130266
GTATAGG3005.456968E-1222.4468961
TACATGG47350.022.2743782
ACATGGG44250.022.1148153
TACACGG2405.9135346E-921.7747962
GAGTACT46450.021.46791812-13
ACAACCG2701.0040822E-921.105495
AGTACTT47950.020.40022312-13
GTATCAA95100.020.1303861
TACAACG1200.004758075319.7952672
GTACTTT50050.019.63914714-15
CATGGGG16750.019.56784
Produced by FastQC (version 0.11.5)