##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936530_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1004236 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60557478520985 31.0 31.0 34.0 30.0 34.0 2 31.868933198969167 33.0 31.0 34.0 30.0 34.0 3 32.156474175393036 33.0 31.0 34.0 30.0 34.0 4 35.9287577820353 37.0 35.0 37.0 35.0 37.0 5 35.776969756113104 37.0 35.0 37.0 35.0 37.0 6 35.88805121505303 37.0 35.0 37.0 35.0 37.0 7 35.82697692574256 37.0 35.0 37.0 35.0 37.0 8 35.81283781899872 37.0 35.0 37.0 35.0 37.0 9 37.48100147774029 39.0 37.0 39.0 35.0 39.0 10-11 37.3735152892348 39.0 37.0 39.0 34.0 39.0 12-13 37.262371096037185 39.0 37.0 39.0 34.0 39.0 14-15 38.42614833565018 40.0 38.0 41.0 34.0 41.0 16-17 38.34545067095782 40.0 38.0 41.0 33.5 41.0 18-19 38.41088449328644 40.0 38.0 41.0 33.5 41.0 20-21 38.48529977017354 40.0 38.0 41.0 34.0 41.0 22-23 38.40518314420116 40.0 38.0 41.0 34.0 41.0 24-25 38.30361787468284 40.0 38.0 41.0 34.0 41.0 26-27 38.11386267769727 40.0 37.5 41.0 33.0 41.0 28-29 38.03428228026081 40.0 37.0 41.0 33.0 41.0 30-31 38.01838113750155 40.0 37.0 41.0 33.0 41.0 32-33 37.8448178515807 40.0 37.0 41.0 33.0 41.0 34-35 37.68144390362425 40.0 37.0 41.0 33.0 41.0 36-37 37.457648401371785 40.0 36.5 41.0 32.5 41.0 38-39 37.30919823627116 40.0 36.0 41.0 32.0 41.0 40-41 37.079766110754846 39.0 36.0 41.0 31.5 41.0 42-43 36.84364233108552 39.0 36.0 41.0 31.0 41.0 44-45 36.57428980837174 39.0 35.5 41.0 30.5 41.0 46-47 36.16391366172891 39.0 35.0 40.5 30.0 41.0 48-49 35.699495437327485 39.0 35.0 40.0 28.0 41.0 50-51 35.53560467858153 39.0 35.0 40.0 28.0 41.0 52-53 35.286351017091604 38.0 35.0 40.0 27.0 41.0 54-55 35.03328699628374 38.0 34.0 40.0 26.5 41.0 56-57 34.77635735026428 38.0 34.0 40.0 26.0 41.0 58-59 34.41233236012252 38.0 34.0 40.0 25.5 41.0 60-61 34.050266072915136 37.5 33.0 40.0 24.0 41.0 62-63 33.78578640877244 37.0 33.0 39.5 24.0 41.0 64-65 33.36905020333866 36.0 32.5 39.0 23.0 40.5 66-67 32.9730053493402 36.0 32.0 39.0 22.0 40.0 68-69 32.52187782553105 35.0 31.5 38.5 21.5 40.0 70-71 32.06527798246627 35.0 31.0 38.0 20.5 40.0 72-73 31.63047580449217 35.0 31.0 37.0 19.0 39.0 74-75 31.272640594441945 34.5 31.0 37.0 18.0 39.0 76-77 29.83992856260879 33.5 28.5 35.5 16.5 37.5 78-79 30.615167151944362 34.0 30.0 36.0 17.5 38.0 80-81 30.442138600886643 34.0 30.0 35.5 14.0 37.0 82-83 30.097172377807606 34.0 30.0 35.0 9.5 37.0 84-85 29.648722013550596 34.0 29.5 35.0 7.0 36.5 86-87 29.267132924929996 34.0 29.0 35.0 3.5 36.0 88-89 28.85235392875778 34.0 29.0 35.0 2.0 35.5 90-91 28.405864756889812 33.5 29.0 35.0 2.0 35.0 92-93 27.978322824515352 33.0 27.5 35.0 2.0 35.0 94-95 27.47295406657399 33.0 27.0 35.0 2.0 35.0 96-97 26.819021624399046 33.0 25.5 34.5 2.0 35.0 98-99 26.000576557701578 32.0 24.5 34.0 2.0 35.0 100-101 23.214113515149826 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2571225173929008 1101 2 0.21198248603927183 1101 3 0.024344086752520866 1101 4 -0.021244049153501976 1101 5 -0.0010981293029033168 1101 6 0.005800479885692766 1101 7 0.03647746878355207 1101 8 -0.040354782038875214 1101 9 0.10250438301930132 1101 10-11 -0.03152871472765639 1101 12-13 -0.0441182284510262 1101 14-15 -0.04824225190751008 1101 16-17 -0.20086902839987175 1101 18-19 0.04779612826942525 1101 20-21 0.007472749339804352 1101 22-23 -0.11001673382165933 1101 24-25 -0.17078668490270132 1101 26-27 -0.24505903225658443 1101 28-29 -0.1501330180203695 1101 30-31 -0.16293916062036828 1101 32-33 -0.26970878543638577 1101 34-35 -0.18420876425818022 1101 36-37 -0.22063306815530837 1101 38-39 -0.1610850706424003 1101 40-41 -0.04982392315333328 1101 42-43 0.18674881731433857 1101 44-45 0.08435891910419002 1101 46-47 0.058907877087563065 1101 48-49 0.019446638477376155 1101 50-51 -0.09393212101995374 1101 52-53 -0.08393606665074316 1101 54-55 -0.14105300580846603 1101 56-57 -0.43372313879000757 1101 58-59 -0.47195965355455627 1101 60-61 -0.36494305851980613 1101 62-63 -0.33537813677553885 1101 64-65 -0.2577760216594598 1101 66-67 -0.34627814705323345 1101 68-69 -0.3355323650613542 1101 70-71 -0.40797971827250734 1101 72-73 -0.3748956568690609 1101 74-75 -0.46512854162066475 1101 76-77 -0.012286479497628733 1101 78-79 -0.19615223782817637 1101 80-81 -0.5508096951179127 1101 82-83 -0.4831958558922871 1101 84-85 -0.42126416640713416 1101 86-87 -0.6361940325856885 1101 88-89 -0.5510271677206262 1101 90-91 -0.5577323882265368 1101 92-93 -0.2752025337693169 1101 94-95 -0.649615056751152 1101 96-97 -0.6447384854517892 1101 98-99 -0.789153404881791 1101 100-101 -0.6926396635203709 1102 1 0.3744043977801823 1102 2 0.4373883151416216 1102 3 0.13212619621732813 1102 4 0.11095274944760547 1102 5 0.0018285057904776636 1102 6 0.10301465154586253 1102 7 0.05468024730983956 1102 8 -0.13519675731401293 1102 9 -0.02675737990193028 1102 10-11 0.10386806112725111 1102 12-13 0.06669196934400645 1102 14-15 0.0873092885984974 1102 16-17 0.19199958448882626 1102 18-19 0.09058903894987225 1102 20-21 0.13560786437291483 1102 22-23 -0.11408617177719549 1102 24-25 0.08830064953304628 1102 26-27 -0.07674585063784178 1102 28-29 -0.11372229137610645 1102 30-31 -0.07003772082447313 1102 32-33 0.010195525421501372 1102 34-35 0.1824299866133856 1102 36-37 0.137355340327737 1102 38-39 -0.039482057804363535 1102 40-41 0.2661192268468895 1102 42-43 0.15059753955282673 1102 44-45 0.1989112550811143 1102 46-47 0.15578117879591957 1102 48-49 0.08492371747713179 1102 50-51 0.23500609008161177 1102 52-53 -0.06942534588688432 1102 54-55 0.10645573564797672 1102 56-57 0.1157792557002395 1102 58-59 0.15192734951036613 1102 60-61 0.1028982039978743 1102 62-63 0.01980027552653496 1102 64-65 -0.17130393842386837 1102 66-67 -0.08374915695235785 1102 68-69 -0.07074070929929377 1102 70-71 -0.22932767638071994 1102 72-73 -0.4388221814624238 1102 74-75 -0.7148879940575128 1102 76-77 -0.3068788927341082 1102 78-79 -0.13184510204691335 1102 80-81 -0.1529873209958552 1102 82-83 -0.17030405738487886 1102 84-85 -0.03674476719123021 1102 86-87 -0.18840723171642182 1102 88-89 -0.22974609120485212 1102 90-91 -0.3629786837294482 1102 92-93 -0.03562814625284716 1102 94-95 -0.19544704114415268 1102 96-97 -0.45586874559210244 1102 98-99 -0.510754091403566 1102 100-101 -0.543678202180196 1103 1 0.29416440961130164 1103 2 0.25115780206874305 1103 3 0.11085185190111702 1103 4 0.0630660324306831 1103 5 -0.19017924053396484 1103 6 -0.07507941606491642 1103 7 0.04838030289571549 1103 8 0.08251307934512653 1103 9 -0.10789490138408553 1103 10-11 -0.09077395822070855 1103 12-13 -0.023988218118063287 1103 14-15 0.015903119076902783 1103 16-17 -0.15140411936598497 1103 18-19 -0.04367506022755663 1103 20-21 0.010195113790281596 1103 22-23 -0.06653794211065645 1103 24-25 -0.20624039731717403 1103 26-27 -0.07672353979554458 1103 28-29 0.04653170800174422 1103 30-31 0.05876277179892497 1103 32-33 -0.10305207903147817 1103 34-35 -0.16730266575054742 1103 36-37 -0.11727575308202631 1103 38-39 -0.2883942448788588 1103 40-41 -0.06519646264737133 1103 42-43 -0.0759183865638633 1103 44-45 0.11256670173143846 1103 46-47 -8.524083285621487E-4 1103 48-49 -0.05732288235049765 1103 50-51 -0.20517057354228285 1103 52-53 -0.07982729802183997 1103 54-55 -0.13604877751669875 1103 56-57 -0.03327852555661792 1103 58-59 -0.031593989854123095 1103 60-61 -0.25983370127156746 1103 62-63 -0.39318042750722526 1103 64-65 -0.26282683827241016 1103 66-67 -0.3709773471478215 1103 68-69 -0.32770424190881897 1103 70-71 -0.38725369872726034 1103 72-73 -0.276277177306234 1103 74-75 -0.27298224015502726 1103 76-77 -0.5728024596130901 1103 78-79 -0.2763725074106951 1103 80-81 -0.3144671417951699 1103 82-83 -0.12506436244421693 1103 84-85 -0.11509375211165107 1103 86-87 -0.3155047696193556 1103 88-89 -0.33297294019996926 1103 90-91 -0.5091889640556175 1103 92-93 -0.47744115079660787 1103 94-95 -0.42761270998495604 1103 96-97 -0.7190799514995057 1103 98-99 -0.8908052661012853 1103 100-101 -0.31649326952069146 1104 1 0.2541386444845557 1104 2 0.35775716461281704 1104 3 0.17400079178419503 1104 4 -0.09761571692903459 1104 5 -0.08930838438241295 1104 6 0.03342666850834064 1104 7 -0.08975375275080211 1104 8 -0.027052223959692867 1104 9 -0.12158070028264945 1104 10-11 -0.1645487674202002 1104 12-13 -0.1285820580971233 1104 14-15 -0.07293139387495984 1104 16-17 -0.09221586200212784 1104 18-19 -0.06929519398039474 1104 20-21 -0.16206137496698858 1104 22-23 -0.026226681929067297 1104 24-25 0.03696514115858207 1104 26-27 0.073510373680854 1104 28-29 -0.09630916667811817 1104 30-31 -0.09452112704985183 1104 32-33 -0.04253707161502973 1104 34-35 -0.011697595871616784 1104 36-37 0.13643824887858358 1104 38-39 0.18522954064442132 1104 40-41 0.004585906717785804 1104 42-43 0.10380716607757279 1104 44-45 0.17969749567714643 1104 46-47 0.31370155216148987 1104 48-49 0.05019295139771174 1104 50-51 0.22578485330169684 1104 52-53 0.12978252968029835 1104 54-55 0.22746245238317186 1104 56-57 0.2592387452902969 1104 58-59 0.3429049502855932 1104 60-61 0.16830975967271655 1104 62-63 0.11202199811008029 1104 64-65 -0.07493901409116432 1104 66-67 -0.12195925766789628 1104 68-69 -0.08231322274921382 1104 70-71 0.21194556783915175 1104 72-73 0.14575797258829226 1104 74-75 0.06469425352943503 1104 76-77 0.05653770678241088 1104 78-79 0.19003948055873465 1104 80-81 0.1895416578681406 1104 82-83 0.3128237825179845 1104 84-85 0.28232402531255474 1104 86-87 0.31856595360057227 1104 88-89 0.11170934550551692 1104 90-91 0.09403995293653722 1104 92-93 0.015003495240847542 1104 94-95 0.1259298024741753 1104 96-97 0.17901627765614947 1104 98-99 0.12159933364106124 1104 100-101 0.25064041145365934 1105 1 0.28526664074850316 1105 2 0.3415926349041065 1105 3 0.07346888289759335 1105 4 0.04809157840030309 1105 5 -0.01668259327838939 1105 6 0.03738061092506939 1105 7 0.09211497662668222 1105 8 0.03168959595657128 1105 9 -0.03173745629610636 1105 10-11 0.032784978667876885 1105 12-13 0.03597654787245119 1105 14-15 0.03816329414424047 1105 16-17 0.028395989329105475 1105 18-19 -0.09644599715815616 1105 20-21 0.02362611079198018 1105 22-23 0.028247975837686568 1105 24-25 -0.03159477216292572 1105 26-27 -0.29368246670728837 1105 28-29 0.09525841338556518 1105 30-31 0.1134102741749956 1105 32-33 0.2447501930632896 1105 34-35 0.142977814818245 1105 36-37 0.26441923381680255 1105 38-39 0.2547487101570738 1105 40-41 0.3782108787339453 1105 42-43 0.25360591492583495 1105 44-45 -0.11234120938014769 1105 46-47 0.3209966157786468 1105 48-49 0.3615486447891456 1105 50-51 0.2502608330350853 1105 52-53 0.33822212410890273 1105 54-55 0.4110822893229056 1105 56-57 0.47925783741144556 1105 58-59 0.49035637797633314 1105 60-61 0.1903185112246959 1105 62-63 0.030321470718469357 1105 64-65 0.21360943332877014 1105 66-67 0.4064533925563367 1105 68-69 -0.08795151009161373 1105 70-71 0.012255532478206987 1105 72-73 -0.10012649284227848 1105 74-75 -0.13588794323367281 1105 76-77 -0.1992943914975065 1105 78-79 -0.07985462260647935 1105 80-81 -0.4358784319060156 1105 82-83 -0.15812151662845153 1105 84-85 -0.05563209469544361 1105 86-87 -0.24719391481392705 1105 88-89 -0.16720867462690592 1105 90-91 -0.3846435011563436 1105 92-93 -0.19556021391851885 1105 94-95 -0.19237285879745158 1105 96-97 -0.0026408706293139517 1105 98-99 -0.12606513661236463 1105 100-101 0.293809265122583 1106 1 0.3315237559583757 1106 2 0.34149081349464794 1106 3 0.14868049587511933 1106 4 0.05010202457469859 1106 5 -0.06775902113054144 1106 6 -0.07872528895850195 1106 7 -0.07278936040358275 1106 8 -0.04335769833258496 1106 9 0.06367271545203579 1106 10-11 -0.13520735593553468 1106 12-13 0.06907377257194014 1106 14-15 0.15769881998510726 1106 16-17 -0.0047479327754089695 1106 18-19 0.008899837503534513 1106 20-21 -2.060678631679025E-4 1106 22-23 0.06697452004713256 1106 24-25 -0.09042086259999849 1106 26-27 -0.09190789509452912 1106 28-29 -0.030542289111934906 1106 30-31 -0.08220691103183242 1106 32-33 0.033184615160074316 1106 34-35 -0.1695637037392359 1106 36-37 -0.14467812289940696 1106 38-39 -0.22474270243591832 1106 40-41 -0.26935924110634346 1106 42-43 -0.21679109026073462 1106 44-45 0.07499508637102537 1106 46-47 0.09535089248851136 1106 48-49 -0.09326687588340832 1106 50-51 -0.04046948629089542 1106 52-53 0.13151939627823594 1106 54-55 -0.0014871774559352957 1106 56-57 -0.029238632545620646 1106 58-59 -0.08569613940001375 1106 60-61 -0.0876170702317296 1106 62-63 -0.3462520211660163 1106 64-65 -0.44199544904292054 1106 66-67 -0.5311943362644556 1106 68-69 -0.2969557528844149 1106 70-71 -0.39893934527290753 1106 72-73 -0.22243612542850144 1106 74-75 -0.4605100453612465 1106 76-77 -0.547509216413836 1106 78-79 -0.5795231763632671 1106 80-81 -0.47540164086742465 1106 82-83 -0.3221754693355656 1106 84-85 -0.28559188577195727 1106 86-87 -0.4899905767570196 1106 88-89 -0.48826772963996135 1106 90-91 -0.43665738819216315 1106 92-93 0.15538974472092093 1106 94-95 0.13176225610625636 1106 96-97 0.12158265334646856 1106 98-99 -0.18155956031020892 1106 100-101 -0.322617196646636 1107 1 0.4436040613084735 1107 2 0.46436501733191093 1107 3 0.2844883621295793 1107 4 0.11084955987639233 1107 5 -0.04050962247968215 1107 6 0.14324752829605103 1107 7 0.028186324899188264 1107 8 0.15864660275954634 1107 9 0.20903355434143833 1107 10-11 0.30956311198957565 1107 12-13 0.3070075820182723 1107 14-15 0.3628701285247544 1107 16-17 0.2937947144938633 1107 18-19 0.18750984371796875 1107 20-21 0.2738616204272333 1107 22-23 0.31065177738535965 1107 24-25 0.37616039647904387 1107 26-27 0.2661526338574589 1107 28-29 0.33013290785808636 1107 30-31 0.3603586100492038 1107 32-33 0.3669794565940592 1107 34-35 0.3002830723040333 1107 36-37 0.4678383688679375 1107 38-39 0.44117035825966866 1107 40-41 0.3602096890901052 1107 42-43 0.16161532961916691 1107 44-45 0.37629551001095507 1107 46-47 0.7242635826706874 1107 48-49 0.8078330000239973 1107 50-51 0.7222686273841887 1107 52-53 0.7473827611280726 1107 54-55 0.7046069839193194 1107 56-57 0.6754205245265794 1107 58-59 0.527246246745527 1107 60-61 0.5345411017453046 1107 62-63 0.5157611408816507 1107 64-65 0.4900555288968249 1107 66-67 0.38012896510800687 1107 68-69 0.511053609930542 1107 70-71 0.36872130365486555 1107 72-73 0.2346975318916904 1107 74-75 0.5879595774404223 1107 76-77 0.5101521774615172 1107 78-79 0.72349601779948 1107 80-81 0.7949148442346043 1107 82-83 0.5275421367165443 1107 84-85 0.5841501075117534 1107 86-87 0.8861633568603118 1107 88-89 0.7828481038289681 1107 90-91 0.8437026567399393 1107 92-93 0.9210561885567756 1107 94-95 1.0448998969356538 1107 96-97 0.6996374775682419 1107 98-99 1.1479381097681873 1107 100-101 1.1278942686848765 1108 1 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-0.7193169605156626 2316 60-61 -0.5455079889872039 2316 62-63 -0.7005765374327808 2316 64-65 -1.0094588613391764 2316 66-67 -0.7798088179804026 2316 68-69 -1.0071722074389804 2316 70-71 -0.6350046476252373 2316 72-73 -0.5657124404727014 2316 74-75 -0.7516442532848444 2316 76-77 -0.844602677900351 2316 78-79 -0.5980784467789668 2316 80-81 -0.5246877503930776 2316 82-83 -0.45646290773882114 2316 84-85 -0.6536147337527147 2316 86-87 -0.6615867983876598 2316 88-89 -0.8441204551461325 2316 90-91 -0.7922018576650132 2316 92-93 -0.876913962629736 2316 94-95 -0.9425721069256809 2316 96-97 -1.115521787718901 2316 98-99 -1.657488358612735 2316 100-101 -2.356662605047756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 9.0 5 18.0 6 44.0 7 135.0 8 327.0 9 678.0 10 1141.0 11 1869.0 12 2818.0 13 3990.0 14 4794.0 15 6456.0 16 8277.0 17 11045.0 18 9317.0 19 5751.0 20 5146.0 21 5365.0 22 5715.0 23 6409.0 24 7488.0 25 8766.0 26 11017.0 27 13523.0 28 17084.0 29 21341.0 30 26680.0 31 33749.0 32 42670.0 33 55827.0 34 74255.0 35 103224.0 36 149441.0 37 206944.0 38 143182.0 39 9732.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05788832223263 19.74920840058106 14.594462181155043 21.59844109603127 2 15.55341990997092 21.455403736605184 40.01463968449986 22.976536668924034 3 19.986078248607825 25.873744254041092 30.09089967575663 24.049277821594448 4 12.948890832060203 17.417531964393486 37.72386030678647 31.90971689675984 5 13.185048136095501 38.10628179033614 34.07754750875292 14.63112256481544 6 30.739387952632647 36.928072684110106 18.05780712900155 14.274732234255694 7 28.379982394576576 33.17526955815167 20.96489271446154 17.479855332810214 8 26.53927961156541 34.457438291397644 19.90338924316595 19.099892853871005 9 26.656967249030057 17.18400213506688 20.73703624037029 35.421994375532776 10-11 24.74134514465752 27.04534003031166 27.512860548437914 20.700454276592907 12-13 26.365678170110495 24.4013978792823 28.101426408447022 21.13149754216018 14-15 22.906739956285133 25.80332292740245 27.016634552669444 24.27330256364297 16-17 21.656114698138683 29.118752962451055 27.585149307533293 21.63998303187697 18-19 21.35624562166942 27.51087767759654 30.366731707851397 20.766144992882644 20-21 22.27066147797928 26.390260855018145 30.469381699122515 20.869695967880062 22-23 21.922088785325926 26.433450837465895 30.240485152655793 21.403975224552386 24-25 22.033416447926584 26.475649150199754 30.576726984493686 20.91420741737998 26-27 21.868774804316317 26.569664187334546 30.59894815508912 20.96261285326002 28-29 21.366989432762818 26.908366160942247 30.719621682552706 21.00502272374223 30-31 21.606972862952535 26.64194472215694 30.482774965247213 21.268307449643313 32-33 21.450002887876693 26.818067751578873 30.359152278733877 21.37277708181056 34-35 22.122239294502137 26.584228466273064 30.206467262879805 21.08706497634499 36-37 22.218543260687397 26.383465186761512 30.20518149368383 21.192810058867256 38-39 21.79955707622511 26.723150733492922 30.438064359373694 21.03922783090827 40-41 21.943121803109644 26.73276249665778 30.272899232572048 21.051216467660527 42-43 21.79435469816069 26.976966061065983 29.72851043742475 21.50016880334858 44-45 21.993741395663786 26.867486376208344 29.542305327282186 21.596466900845684 46-47 22.431279724680746 27.27187178853952 28.889701020497583 21.407147466282154 48-49 22.552056594632035 27.382687488142643 28.538421817634983 21.52683409959034 50-51 22.18979279688359 27.577243107170457 28.76800380788503 21.464960288060926 52-53 22.488748679939714 27.764060602530034 28.375632521107274 21.37155819642298 54-55 22.025652784645178 27.57592189539213 28.513191132117466 21.885234187845228 56-57 22.371660724146054 27.853368366073212 28.134287522399593 21.640683387381145 58-59 22.732962302423054 27.420538542503753 28.13038659508937 21.716112559983827 60-61 22.322812513848316 27.552633557512145 28.702921328621713 21.421632600017826 62-63 22.31660331626175 27.438973992310316 28.826243299412567 21.41817939201536 64-65 22.175473646783942 27.68688668430818 28.494996867751382 21.64264280115649 66-67 22.116197055117098 27.758705418602464 28.194667185811028 21.93043034046941 68-69 22.165091918272076 27.62537036917174 28.374099874083196 21.835437838472988 70-71 22.307423061584092 27.744781192726553 28.088627239716857 21.859168505972495 72-73 22.32783047402726 27.513068398796896 28.29485713261481 21.864243994561036 74-75 22.1478317845606 28.125012447273352 27.921175898892294 21.805979869273756 76-77 22.33601333561701 27.754503957268668 28.11079754796719 21.798685159147126 78-79 22.31117179400614 27.66208856051077 28.041821403069534 21.98491824241356 80-81 22.387323908594308 28.070800073698205 27.756985145827834 21.784890871879654 82-83 22.292565775288697 28.086099313876485 27.83836983546281 21.782965075372005 84-85 22.235754788569974 27.969142429209633 27.737954632818212 22.057148149402188 86-87 22.210293804238063 28.006708241297513 27.716608673309846 22.06638928115458 88-89 22.346919616332226 27.85705143178163 27.513206695808474 22.28282225607767 90-91 22.166062824487355 27.734857256688954 27.810704814551972 22.288375104271715 92-93 22.298866026861845 27.836887113036333 27.761753612905636 22.102493247196186 94-95 22.031812896945436 27.643908669250933 27.803744949098867 22.520533484704764 96-97 22.02482733895343 27.63682174209643 27.915270357073503 22.423080561876642 98-99 21.962629619947467 27.835580286564337 27.475221271987156 22.72656882150104 100-101 21.560860524313537 28.280943142933403 27.455155298307393 22.70304103444567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 252.0 1 191.0 2 268.5 3 371.0 4 267.5 5 234.5 6 340.0 7 574.5 8 880.5 9 1060.5 10 1010.5 11 927.5 12 963.5 13 1064.0 14 1198.5 15 1318.5 16 1460.0 17 1566.0 18 1618.5 19 1775.5 20 1911.0 21 2129.5 22 2473.5 23 2870.5 24 3412.0 25 4174.5 26 5296.0 27 6694.5 28 8211.5 29 9946.5 30 12127.5 31 14649.5 32 17682.5 33 20413.5 34 22771.0 35 25039.5 36 28004.0 37 30423.0 38 31424.5 39 32139.0 40 32424.5 41 33403.0 42 34469.5 43 35303.0 44 36439.0 45 37620.5 46 37937.5 47 39576.5 48 45800.0 49 44808.5 50 37696.0 51 33822.5 52 30911.5 53 29653.0 54 28270.5 55 25866.0 56 23227.0 57 20738.5 58 18786.0 59 16678.5 60 13970.0 61 11345.0 62 9156.0 63 7014.5 64 4980.5 65 3724.0 66 2761.0 67 2138.5 68 1667.0 69 1159.0 70 816.0 71 614.5 72 538.0 73 468.5 74 369.5 75 329.5 76 260.0 77 157.0 78 99.0 79 76.0 80 56.5 81 37.5 82 26.0 83 15.0 84 7.0 85 4.5 86 2.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2205298356163292 2 0.011551069668882613 3 0.005178065713637033 4 0.014936728020106826 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005178065713637033 10-11 1.9915637360142435E-4 12-13 4.978909340035609E-5 14-15 9.957818680071217E-5 16-17 0.0 18-19 4.978909340035609E-5 20-21 0.0 22-23 0.0015932509888113948 24-25 0.0 26-27 4.978909340035609E-5 28-29 0.0 30-31 0.0 32-33 0.003783971098427063 34-35 8.962036812064097E-4 36-37 0.002738400137019585 38-39 0.0 40-41 0.0037341820050267064 42-43 0.010854022361277629 44-45 0.0018421964558131753 46-47 0.001991563736014244 48-49 0.01120254601508012 50-51 3.983127472028487E-4 52-53 0.0020413528294145997 54-55 0.009509716839468014 56-57 0.0032362910710231458 58-59 6.970473076049852E-4 60-61 0.00522785480703739 62-63 0.0032860801644235024 64-65 0.01643040082211751 66-67 0.0019417746426138875 68-69 1.493672802010683E-4 70-71 7.468364010053414E-4 72-73 4.978909340035609E-5 74-75 0.0 76-77 7.966254944056974E-4 78-79 0.010854022361277629 80-81 0.014040524338900417 82-83 0.01160085876228297 84-85 0.007368785823252701 86-87 0.009758662306469794 88-89 0.02932577601280974 90-91 0.02474517941997698 92-93 0.002340087389816736 94-95 0.008314778597859467 96-97 0.010057396866871932 98-99 0.00980845139987015 100-101 0.010356131427274066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1004236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.75463615934645 #Duplication Level Percentage of deduplicated Percentage of total 1 84.64861346709719 55.66038779922129 2 9.681385815293764 12.73192003605798 3 2.434350598861102 4.802095137371967 4 0.9914635168873887 2.607732912727851 5 0.5198511240984034 1.7091310761058889 6 0.33685516577318203 1.3289873318287149 7 0.2201210258409103 1.0131784575633807 8 0.1642690866473912 0.8641163219781891 9 0.13057055119442704 0.772705717822338 >10 0.7781565727765671 9.657786863090323 >50 0.059975166530542766 2.678279831097157 >100 0.0314968907028371 3.6920010281061675 >500 0.001673747434731878 0.7362790125400741 >1k 0.0012172708616231842 1.7453984744885982 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4162 0.41444441346456407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3651 0.36355996000940016 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2795 0.2783210321079906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2143 0.21339605431392622 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1273 0.1267630317973066 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1231 0.1225807479516767 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.10983474004118553 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1087 0.10824148905237414 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.957818680071219E-5 0.0 14-15 0.0 0.0 0.0 9.957818680071219E-5 0.0 16-17 0.0 0.0 0.0 9.957818680071219E-5 0.0 18-19 0.0 0.0 0.0 9.957818680071219E-5 0.0 20-21 0.0 0.0 0.0 1.493672802010683E-4 0.0 22-23 0.0 0.0 0.0 4.481018406032048E-4 0.0 24-25 0.0 4.9789093400356094E-5 0.0 7.468364010053414E-4 0.0 26-27 0.0 9.957818680071219E-5 0.0 0.0011451491482081901 0.0 28-29 0.0 9.957818680071219E-5 0.0 0.0024894546700178046 0.0 30-31 0.0 9.957818680071219E-5 0.0 0.004481018406032048 0.0 32-33 0.0 9.957818680071219E-5 0.0 0.007966254944056974 0.0 34-35 0.0 9.957818680071219E-5 0.0 0.01045570961407478 0.0 36-37 0.0 9.957818680071219E-5 0.0 0.014588204366304335 0.0 38-39 0.0 9.957818680071219E-5 0.0 0.02200677928295739 0.0 40-41 0.0 9.957818680071219E-5 0.0 0.031765441589427186 0.0 42-43 0.0 9.957818680071219E-5 0.0 0.040378954747688794 0.0 44-45 0.0 1.493672802010683E-4 0.0 0.044611027686719054 0.0 46-47 0.0 1.9915637360142438E-4 0.0 0.051382344389167486 0.0 48-49 0.0 1.9915637360142438E-4 0.0 0.05999585754742909 0.0 50-51 0.0 1.9915637360142438E-4 0.0 0.06885831617269247 0.0 52-53 0.0 1.9915637360142438E-4 0.0 0.08230137139078862 0.0 54-55 0.0 1.9915637360142438E-4 0.0 0.09360349559266945 0.0 56-57 0.0 1.9915637360142438E-4 0.0 0.1218837006440717 0.0 58-59 0.0 1.9915637360142438E-4 0.0 0.1680381902262018 0.0 60-61 0.0 1.9915637360142438E-4 0.0 0.20612684667747422 0.0 62-63 0.0 1.9915637360142438E-4 0.0 0.22808383686703126 0.0 64-65 0.0 2.987345604021366E-4 0.0 0.27369064642175744 0.0 66-67 0.0 3.9831274720284875E-4 0.0 0.3657008910256155 0.0 68-69 0.0 3.9831274720284875E-4 0.0 0.4846470351590662 0.0 70-71 0.0 3.9831274720284875E-4 0.0 0.5917433750632322 0.0 72-73 0.0 3.9831274720284875E-4 0.0 0.7120836138118929 0.0 74-75 0.0 3.9831274720284875E-4 0.0 0.856272828299324 0.0 76-77 0.0 3.9831274720284875E-4 0.0 0.947586025595577 0.0 78-79 0.0 3.9831274720284875E-4 0.0 1.0472140014896896 0.0 80-81 0.0 3.9831274720284875E-4 0.0 1.1677533966119518 0.0 82-83 0.0 3.9831274720284875E-4 0.0 1.297254828546278 0.0 84-85 0.0 3.9831274720284875E-4 0.0 1.457774865669026 0.0 86-87 0.0 3.9831274720284875E-4 0.0 1.7476967565392996 0.0 88-89 0.0 4.481018406032048E-4 0.0 2.1472044419837566 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1180 0.0 38.651096 2 GTACCTG 1255 0.0 37.560543 1 GTACACG 155 3.092282E-11 34.135834 1 TATCACG 95 3.486075E-5 30.005459 2 TGTACGG 100 4.9614933E-5 28.49241 5 ACCTGGG 1695 0.0 27.185127 3 ACACGGG 255 1.8189894E-11 24.217678 3 GTACATG 4495 0.0 23.862982 1 GTACGGG 105 0.0021994312 22.613026 6 GTATAGG 300 5.456968E-12 22.446896 1 TACATGG 4735 0.0 22.274378 2 ACATGGG 4425 0.0 22.114815 3 TACACGG 240 5.9135346E-9 21.774796 2 GAGTACT 4645 0.0 21.467918 12-13 ACAACCG 270 1.0040822E-9 21.10549 5 AGTACTT 4795 0.0 20.400223 12-13 GTATCAA 9510 0.0 20.130386 1 TACAACG 120 0.0047580753 19.795267 2 GTACTTT 5005 0.0 19.639147 14-15 CATGGGG 1675 0.0 19.5678 4 >>END_MODULE