FastQCFastQC Report
Thu 26 May 2016
SRR936529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051573
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.3132450148491831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.2507671840186083No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22590.21482103477362008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.15434021223443356No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14870.14140720615687166No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12010.11420985514082235No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10740.10213270975956971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13750.039.4046672
GTACCTG13850.038.7751541
TACGCCG500.001611155338.022055
CGCACCG500.001611155338.022055
TATCACG753.0678153E-431.685042
ACCTGGG18850.027.9870513
GTACATG47250.026.0509031
TACATGG48550.025.5503462
TAACGCA950.001221666325.0168864
ACATGGG49000.024.0547643
GAGTACT33500.022.4869212-13
AGTACTT35100.020.78484512-13
GTACTTT37050.020.52472314-15
GTATCAA81750.020.4053571
CATGGGA31000.020.3927974
CATGGGG18450.020.3524934
ATCACGC1250.006010636719.0110233
ATGGGAT12100.018.8539095
CGACGAG901.232279E-418.47546694-95
GTACTGG6450.018.4206581
Produced by FastQC (version 0.11.5)