##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936529_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1051573 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.338741104992234 31.0 31.0 34.0 30.0 34.0 2 31.386981217661543 31.0 31.0 34.0 28.0 34.0 3 31.43060824117774 31.0 31.0 34.0 28.0 34.0 4 35.05873867054403 37.0 35.0 37.0 32.0 37.0 5 35.03691041896283 37.0 35.0 37.0 32.0 37.0 6 35.15487560064779 37.0 35.0 37.0 32.0 37.0 7 35.019283492444174 37.0 35.0 37.0 32.0 37.0 8 34.96596432202044 37.0 35.0 37.0 32.0 37.0 9 36.4444779392396 39.0 37.0 39.0 32.0 39.0 10-11 36.34181982610812 39.0 37.0 39.0 32.0 39.0 12-13 36.24203312561278 39.0 35.0 39.0 32.0 39.0 14-15 37.28277732501691 40.0 37.0 41.0 32.0 41.0 16-17 37.15812882225009 39.0 36.0 41.0 31.0 41.0 18-19 37.109883954799145 39.0 36.0 41.0 31.0 41.0 20-21 37.02448855191223 39.0 36.0 41.0 31.0 41.0 22-23 36.86769106852306 39.0 36.0 41.0 30.5 41.0 24-25 37.01997578865186 39.0 36.0 41.0 31.0 41.0 26-27 36.99464468943192 39.0 36.0 41.0 31.0 41.0 28-29 36.90155319697253 39.0 36.0 41.0 30.5 41.0 30-31 36.708204756112984 39.0 36.0 41.0 30.0 41.0 32-33 36.53374373438649 39.0 35.5 41.0 30.0 41.0 34-35 36.29348414232773 39.0 35.0 40.0 30.0 41.0 36-37 36.100002567582095 39.0 35.0 40.0 29.5 41.0 38-39 35.89732809800176 38.0 35.0 40.0 29.0 41.0 40-41 35.72613075839718 38.0 35.0 40.0 28.0 41.0 42-43 35.506719457422356 38.0 35.0 40.0 27.0 41.0 44-45 35.25010722032612 38.0 34.5 40.0 26.5 41.0 46-47 34.97481344614211 38.0 34.0 40.0 25.0 41.0 48-49 34.885605183853144 38.0 34.0 40.0 24.0 41.0 50-51 34.00794714204339 37.5 33.0 39.5 23.5 40.5 52-53 34.17308736530892 38.0 33.5 39.5 24.0 40.5 54-55 34.59251140909856 38.0 34.0 40.0 24.0 41.0 56-57 34.51864112144378 38.0 34.0 40.0 23.5 41.0 58-59 34.26393840465664 38.0 34.0 40.0 23.0 41.0 60-61 33.942261260036155 38.0 33.0 40.0 22.5 41.0 62-63 33.616587721442066 37.0 33.0 40.0 22.0 41.0 64-65 33.2770421073953 37.0 32.5 39.5 21.0 41.0 66-67 32.891956621176085 36.0 32.0 39.0 20.0 41.0 68-69 32.4845089213968 36.0 31.5 39.0 18.5 40.5 70-71 32.03220984182743 35.0 31.0 38.5 17.0 40.0 72-73 31.61973538689183 35.0 31.0 38.0 13.5 40.0 74-75 31.170373335945293 35.0 30.5 37.0 11.0 39.0 76-77 30.722432489232794 35.0 30.0 36.5 7.5 39.0 78-79 30.265486561560635 34.0 30.0 36.0 7.0 39.0 80-81 29.766270625054084 34.0 29.0 36.0 2.0 37.5 82-83 29.26013030003623 34.0 29.0 35.0 2.0 37.0 84-85 28.908694879005072 34.0 29.0 35.0 2.0 36.5 86-87 28.567686694123946 34.0 29.0 35.0 2.0 36.0 88-89 28.142089517323097 34.0 27.5 35.0 2.0 36.0 90-91 27.69931521634732 33.5 26.5 35.0 2.0 35.0 92-93 27.304309829179715 33.0 26.0 35.0 2.0 35.0 94-95 26.832239416569273 33.0 25.0 35.0 2.0 35.0 96-97 26.230928808556325 33.0 24.5 35.0 2.0 35.0 98-99 25.48481275194399 33.0 21.5 35.0 2.0 35.0 100-101 22.956449052990138 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4138130513647731 1101 2 0.4185821961269731 1101 3 0.3522753550152622 1101 4 0.37217780681241663 1101 5 0.4726859889504951 1101 6 0.32433761393693317 1101 7 0.373927960587757 1101 8 0.20809711892348304 1101 9 0.49258943562336555 1101 10-11 0.3927373682764639 1101 12-13 0.5722102769148947 1101 14-15 0.5648724607162947 1101 16-17 0.6058670367165817 1101 18-19 0.5062534554942246 1101 20-21 0.5294155459212178 1101 22-23 0.457230141633282 1101 24-25 0.692558143132878 1101 26-27 0.527079299028351 1101 28-29 0.4847466382072696 1101 30-31 0.419409730131413 1101 32-33 0.4244947695429886 1101 34-35 0.24497368877860737 1101 36-37 0.24501403331134242 1101 38-39 0.3129200328618609 1101 40-41 0.3428658403772431 1101 42-43 0.608694858157655 1101 44-45 0.45124693008428807 1101 46-47 0.4475431474149545 1101 48-49 0.5171335456021708 1101 50-51 0.5719208199842214 1101 52-53 0.5978415410231293 1101 54-55 0.4751352223296905 1101 56-57 0.41797196198582043 1101 58-59 0.47561786791955996 1101 60-61 0.46072792395626294 1101 62-63 0.45248737890977964 1101 64-65 0.3836253831690257 1101 66-67 0.28309292654257234 1101 68-69 0.4008047319699699 1101 70-71 0.44804118212254096 1101 72-73 0.3383587772475032 1101 74-75 0.1642284261873428 1101 76-77 0.1939741978620404 1101 78-79 0.1861088778718809 1101 80-81 0.5196755829492794 1101 82-83 0.5588553117728878 1101 84-85 0.6740556330503047 1101 86-87 0.6793747663395671 1101 88-89 0.7553546147325321 1101 90-91 0.64107939651009 1101 92-93 0.7185623380411954 1101 94-95 0.5233896586085969 1101 96-97 0.4874611641490212 1101 98-99 0.36450172539646175 1101 100-101 0.9182190204365632 1102 1 0.23843722506470044 1102 2 0.20804697805279204 1102 3 0.20687070529745455 1102 4 0.24159391685919474 1102 5 0.22530339691404322 1102 6 0.145366627209512 1102 7 0.26457502042655534 1102 8 0.11534439623238057 1102 9 0.42907608537569075 1102 10-11 0.44480333420614926 1102 12-13 0.3232786419194653 1102 14-15 0.30728775956508514 1102 16-17 0.31460035969418954 1102 18-19 0.554947771930479 1102 20-21 0.5487841922561572 1102 22-23 0.43729797159237904 1102 24-25 0.29606786887892156 1102 26-27 0.2418776214018834 1102 28-29 0.480562064776592 1102 30-31 0.3342477494275684 1102 32-33 0.31052231575538514 1102 34-35 0.0811859119788707 1102 36-37 0.13149982008708605 1102 38-39 0.02073428578303549 1102 40-41 -0.10609340986870563 1102 42-43 0.15831773739271426 1102 44-45 -0.09257156868165595 1102 46-47 0.07091169019270893 1102 48-49 0.31675987674354644 1102 50-51 0.29884482626669495 1102 52-53 0.36915878847302963 1102 54-55 0.345088103808493 1102 56-57 0.2937697838329356 1102 58-59 0.4690765054521364 1102 60-61 0.4356541394587694 1102 62-63 0.42654112509111286 1102 64-65 0.29964826930790167 1102 66-67 0.5390250275453425 1102 68-69 0.4775000494590529 1102 70-71 0.428085711714008 1102 72-73 0.5209301022617403 1102 74-75 0.19429798209959515 1102 76-77 0.4167541906130374 1102 78-79 0.283851331141701 1102 80-81 0.5585690743799212 1102 82-83 0.5075280537946689 1102 84-85 0.3632313178222226 1102 86-87 0.27143365586130486 1102 88-89 0.41958509851720294 1102 90-91 0.7738604248329786 1102 92-93 0.6786263709033413 1102 94-95 0.5946948243863979 1102 96-97 0.5378253402652788 1102 98-99 0.6948879937131416 1102 100-101 0.5890823422060123 1103 1 0.16638306199930142 1103 2 0.3076752164234655 1103 3 0.369270146823947 1103 4 0.4556349823911816 1103 5 0.4393394760754461 1103 6 0.23292840280067395 1103 7 0.33758878280936955 1103 8 0.14614077501924783 1103 9 0.4788384017110374 1103 10-11 0.4766512831457064 1103 12-13 0.482283196137935 1103 14-15 0.29625181337355855 1103 16-17 0.4650868078470225 1103 18-19 0.4143659178870891 1103 20-21 0.40224944279356833 1103 22-23 0.45259117018289174 1103 24-25 0.5381353843366696 1103 26-27 0.28071673892510063 1103 28-29 0.09623533561408237 1103 30-31 0.2154120115555287 1103 32-33 0.3627980398872026 1103 34-35 0.3448518916344838 1103 36-37 0.37449507749461475 1103 38-39 0.5369114570704028 1103 40-41 0.5246049036299567 1103 42-43 0.6217244257777992 1103 44-45 0.6777265608753353 1103 46-47 0.7111447753155957 1103 48-49 0.7503807574474024 1103 50-51 0.8250066506134814 1103 52-53 0.9156855043385548 1103 54-55 0.8814548125822839 1103 56-57 0.9883279913989185 1103 58-59 0.6517175643356339 1103 60-61 0.5815775044832563 1103 62-63 0.7145477957468742 1103 64-65 0.7013483446574966 1103 66-67 0.7205809967849817 1103 68-69 0.7134484571447146 1103 70-71 0.9666101338490556 1103 72-73 0.7206910334941519 1103 74-75 0.6391509395713939 1103 76-77 0.6238550593496015 1103 78-79 0.9625861227114072 1103 80-81 0.685381620088318 1103 82-83 0.6208713044650587 1103 84-85 0.7377094110341069 1103 86-87 1.0469879740582329 1103 88-89 0.8085840569197913 1103 90-91 0.8685344270048212 1103 92-93 0.9090606837814761 1103 94-95 0.8951760091480487 1103 96-97 1.009500539166062 1103 98-99 1.3470928005743836 1103 100-101 1.6745088431035562 1104 1 0.056236513826739554 1104 2 0.23687246111862237 1104 3 0.17668063110748378 1104 4 0.3134811045463479 1104 5 0.38010110100810834 1104 6 0.21113292736797717 1104 7 0.3115614289614044 1104 8 0.3540217572587139 1104 9 0.30509200656067037 1104 10-11 0.4978599094320231 1104 12-13 0.36910534858968447 1104 14-15 0.38651170209293184 1104 16-17 0.2805162433400241 1104 18-19 0.5372992128894154 1104 20-21 0.4906681418333392 1104 22-23 0.148831374272838 1104 24-25 0.4170585585963451 1104 26-27 -0.021626300149883093 1104 28-29 0.42439719053275127 1104 30-31 0.31559910155339566 1104 32-33 -0.07423621580472428 1104 34-35 -0.09993562030623337 1104 36-37 0.07799478095753898 1104 38-39 0.27113245868664393 1104 40-41 0.24380013368387665 1104 42-43 0.10292714395891522 1104 44-45 0.05910465470781645 1104 46-47 0.056142183445686555 1104 48-49 0.30442124494530276 1104 50-51 0.228054870711901 1104 52-53 0.3056628905565901 1104 54-55 0.555192153165315 1104 56-57 0.3581878266963372 1104 58-59 0.38599176153505965 1104 60-61 0.2663812240995256 1104 62-63 0.29879997902942534 1104 64-65 0.3459483704630131 1104 66-67 0.4833977368690938 1104 68-69 0.37794278219462996 1104 70-71 0.5717294164556925 1104 72-73 0.5469596349709498 1104 74-75 0.1694384673575975 1104 76-77 0.38320135354155127 1104 78-79 0.23559604198993966 1104 80-81 0.28823216766327775 1104 82-83 0.24882054682413468 1104 84-85 0.24624843395574558 1104 86-87 0.3742112664800139 1104 88-89 0.3957116312464706 1104 90-91 0.39873197502798163 1104 92-93 0.2895304913266479 1104 94-95 0.2924527914264452 1104 96-97 0.5199246098279069 1104 98-99 0.8412777876489912 1104 100-101 1.2843248995678422 1105 1 0.26005255040563213 1105 2 0.3360848971610828 1105 3 0.31449395135949487 1105 4 0.26649938789121563 1105 5 0.3142179713992874 1105 6 0.352887728225582 1105 7 0.21246491881910856 1105 8 0.2811867344579184 1105 9 0.44710916603443707 1105 10-11 0.234289562545122 1105 12-13 0.2466107208399464 1105 14-15 0.3022951964412357 1105 16-17 0.37025417758929535 1105 18-19 0.3039110893148447 1105 20-21 0.36440073616956425 1105 22-23 0.2366089405366978 1105 24-25 0.3564723274205761 1105 26-27 0.4371422129113043 1105 28-29 0.25764663012253664 1105 30-31 0.28123805460828777 1105 32-33 0.29225985674780475 1105 34-35 0.1907839622629055 1105 36-37 0.10858976148808352 1105 38-39 0.22274224234450912 1105 40-41 -0.003388358168976424 1105 42-43 0.18594259627127485 1105 44-45 0.008334296594405544 1105 46-47 0.373768167443032 1105 48-49 0.41788616027668724 1105 50-51 0.5430969274082074 1105 52-53 0.26963098530832497 1105 54-55 0.1527346461090886 1105 56-57 0.16416295265897674 1105 58-59 0.39360565263060465 1105 60-61 0.22149898651000655 1105 62-63 0.2178354999455081 1105 64-65 0.30375378334836256 1105 66-67 0.5015422757219667 1105 68-69 0.5369717624493902 1105 70-71 0.6862062428682485 1105 72-73 0.580658073876176 1105 74-75 0.6920821514094904 1105 76-77 0.4101855473501317 1105 78-79 0.4950998303263283 1105 80-81 0.28089314297838186 1105 82-83 0.41863856756632956 1105 84-85 0.7748391166860813 1105 86-87 0.9163344676456155 1105 88-89 0.817087024936928 1105 90-91 0.7511125438946777 1105 92-93 0.703848133174187 1105 94-95 0.6057748738461051 1105 96-97 0.8626403143706156 1105 98-99 0.8433656741245876 1105 100-101 1.281772699355269 1106 1 -0.011744366128127126 1106 2 0.023529518816232553 1106 3 0.057538431246079824 1106 4 0.15428070988306075 1106 5 0.1071915670044632 1106 6 -0.10692974474978456 1106 7 0.16054218268585174 1106 8 0.12501980197519913 1106 9 0.2893473372199793 1106 10-11 0.20064168021303175 1106 12-13 0.511053393177356 1106 14-15 0.5863196515183233 1106 16-17 0.4183447322587952 1106 18-19 0.44669861368132047 1106 20-21 0.26035590186157265 1106 22-23 -0.05366219183969889 1106 24-25 -0.0025787092620390695 1106 26-27 -0.11963166623099397 1106 28-29 0.32430000598644426 1106 30-31 -0.04892763739950823 1106 32-33 -0.12265685422153894 1106 34-35 0.15689241521346986 1106 36-37 0.10486833671402707 1106 38-39 0.08863804245083173 1106 40-41 -0.08136106772063556 1106 42-43 -0.26388462329278894 1106 44-45 0.08473426115227056 1106 46-47 0.18667796719493168 1106 48-49 0.24023242999138006 1106 50-51 -0.1414901716525705 1106 52-53 0.062914699644665 1106 54-55 0.2204512861941481 1106 56-57 0.2015101084271933 1106 58-59 0.12428968594621637 1106 60-61 0.2245780224837759 1106 62-63 0.3035168751005699 1106 64-65 0.1307296826926816 1106 66-67 0.32989155801861614 1106 68-69 0.29935436035830776 1106 70-71 0.20640826307026927 1106 72-73 0.3715051410390302 1106 74-75 0.13404564600456226 1106 76-77 0.17908063861364099 1106 78-79 0.3784508847300181 1106 80-81 0.4558887127107951 1106 82-83 0.5443031077045539 1106 84-85 0.21385648333502516 1106 86-87 0.15694230035871115 1106 88-89 0.22582351262078504 1106 90-91 0.46722099684523855 1106 92-93 0.6639978762186693 1106 94-95 0.6794723751751839 1106 96-97 0.753877245081231 1106 98-99 0.9498250042681278 1106 100-101 1.4335315155877666 1107 1 0.16816397807753702 1107 2 0.1225819166094908 1107 3 -0.04355509267441349 1107 4 0.10278347377981589 1107 5 0.215594168705195 1107 6 0.2868161353892731 1107 7 0.41169027339527275 1107 8 0.3997512759349462 1107 9 0.45411647457238047 1107 10-11 0.44317498783569675 1107 12-13 0.3407769761503303 1107 14-15 0.21139452699937067 1107 16-17 0.24308516392449064 1107 18-19 0.30390525906830845 1107 20-21 0.39166458851144625 1107 22-23 0.6528402821282526 1107 24-25 0.5391925779397866 1107 26-27 0.25262495084690073 1107 28-29 0.2707332662905415 1107 30-31 0.34959899091997215 1107 32-33 0.11064249453271913 1107 34-35 0.46724000155618484 1107 36-37 0.45571842984681865 1107 38-39 0.3110941223398953 1107 40-41 0.31012829107173445 1107 42-43 0.37477553638687056 1107 44-45 0.64501244236871 1107 46-47 0.5874026524444034 1107 48-49 0.46555976234551366 1107 50-51 0.5394640360075655 1107 52-53 0.6826997420535932 1107 54-55 0.7323175114031812 1107 56-57 0.7589611419139644 1107 58-59 0.8794413925601461 1107 60-61 0.8725541963535335 1107 62-63 1.0244986943764545 1107 64-65 1.1342043836694202 1107 66-67 1.0640538552394005 1107 68-69 0.9634400452603984 1107 70-71 1.0724283338019482 1107 72-73 1.0490762761649997 1107 74-75 1.175930101170902 1107 76-77 0.9558405885051897 1107 78-79 1.0807863159444082 1107 80-81 1.1879541545650234 1107 82-83 1.4234038224064065 1107 84-85 1.2695429855081244 1107 86-87 0.9742987787631208 1107 88-89 0.9300564731494418 1107 90-91 0.990976012476775 1107 92-93 1.0933581592548265 1107 94-95 1.3117322197624013 1107 96-97 1.663413988086699 1107 98-99 1.5133419774114216 1107 100-101 2.146727994320397 1108 1 -0.03437552197727456 1108 2 0.09508999089573322 1108 3 0.048873349051586246 1108 4 0.2702093154310461 1108 5 0.23016583098218746 1108 6 0.05200970744226652 1108 7 -0.06276645152244242 1108 8 0.03861072854190439 1108 9 0.1894119976652604 1108 10-11 -0.08463175774206633 1108 12-13 0.08473426184092858 1108 14-15 0.1506262119321491 1108 16-17 0.32897933666502155 1108 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>>Per sequence quality scores pass #Quality Count 2 2654.0 3 1049.0 4 1099.0 5 1128.0 6 1211.0 7 1438.0 8 1830.0 9 2265.0 10 2858.0 11 3701.0 12 4579.0 13 5438.0 14 6034.0 15 7140.0 16 8335.0 17 10196.0 18 8891.0 19 6853.0 20 6966.0 21 7613.0 22 8777.0 23 9392.0 24 10866.0 25 12524.0 26 14759.0 27 17253.0 28 20362.0 29 24102.0 30 29655.0 31 36259.0 32 45122.0 33 58166.0 34 75752.0 35 103088.0 36 141599.0 37 185149.0 38 146451.0 39 21016.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82218349384891 18.746551546999797 14.001385088984355 22.429879870166932 2 15.14316777305461 20.262926856643894 40.65340776681483 23.94049760348667 3 19.431323348406476 25.53908522445159 29.684453229184697 25.34513819795723 4 12.421536994765802 16.47159346624509 38.21011576891129 32.89675377007782 5 13.2405079671987 37.61844226183566 34.393120785891405 14.74792898507423 6 30.593936187824806 36.104001628890735 18.868872392775792 14.433189790508665 7 28.337671926259144 32.62802654914408 21.223996985819653 17.810304538777125 8 26.336028922081628 33.83978688992484 20.51907525449529 19.305108933498243 9 26.514943215495695 17.022126785902866 20.977395609654458 35.485534388946974 10-11 24.462388355371264 26.60303465966883 28.050858665773543 20.883718319186368 12-13 25.99520296993436 24.0746308275305 28.157196448941885 21.77296975359325 14-15 22.709201249323346 25.517610112555168 26.961010494430276 24.812178143691213 16-17 21.60619422507251 28.834296582396448 27.53934542195447 22.020163770576577 18-19 21.39639789647354 27.576767583542107 29.78828674106586 21.238547778918495 20-21 22.379644890727196 26.465712619988945 29.56909071368994 21.585551775593917 22-23 21.871012569071873 26.577578076959114 29.50419484272847 22.047214511240544 24-25 21.936791971941357 26.625231095793485 29.76399698719558 21.673979945069576 26-27 21.75509575220835 26.64199630229851 29.829647044501762 21.773260900991378 28-29 21.314759506635898 27.081198517758416 29.935840222992432 21.668201752613253 30-31 21.590130421736735 26.732628069085905 29.785534300198723 21.891707208978637 32-33 21.36828541132725 26.833486284336704 29.693685897283494 22.104542407052545 34-35 22.077555253446164 26.794646291876667 29.415960410672227 21.711838044004946 36-37 22.08200782702559 26.44811861319943 29.538557373619213 21.93131618615577 38-39 21.746246867436074 26.824971111459835 29.637074339843362 21.791707681260732 40-41 21.841626773327853 26.715746409599504 29.691960660127776 21.750666156944863 42-43 21.559551234959738 26.956809098927536 29.189032406846067 22.294607259266655 44-45 21.78755170938139 26.960819742285196 29.017783272312304 22.23384527602111 46-47 22.071902013180292 27.123919472788305 28.636990404823266 22.167188109208137 48-49 22.075688536882602 27.266788041951024 28.377709487670455 22.279813933495916 50-51 21.883395193277856 27.490255037948057 28.537404583056365 22.088945185717723 52-53 22.18750579509679 27.64376476115167 28.160532858852598 22.00819658489894 54-55 21.747697926053082 27.303013083721073 28.429168875817584 22.52012011440826 56-57 22.077510134126182 27.734454507237032 27.907414699286093 22.280620659350692 58-59 22.27607822027616 27.231775810261194 28.095712036474772 22.39643393298788 60-61 22.004928343108546 27.384021782858074 28.633892202242766 21.97715767179061 62-63 21.994529802684887 27.335199844795515 28.629569117350567 22.04070123516903 64-65 21.824410585099933 27.581930870196775 28.323582064004142 22.270076480699146 66-67 21.738435772997928 27.616531478454053 28.07226268833863 22.572770060209397 68-69 21.814300361617903 27.52566612072065 28.30454276545436 22.355490752207086 70-71 21.844093039180024 27.52891722955703 28.105621295444333 22.52136843581861 72-73 21.872597272855657 27.330547259518067 28.30730562190008 22.489549845726195 74-75 21.65983287930611 27.839627661218497 28.042858141415888 22.457681318059507 76-77 21.86848835119227 27.50154306164353 28.102980462794857 22.526988124369343 78-79 21.7914615230257 27.53202808561872 28.098647740035894 22.577862651319688 80-81 21.83532264967426 27.828237196252793 27.9268629036093 22.409577250463645 82-83 21.92729434141702 27.768854018069423 28.03699476937708 22.266856871136472 84-85 21.714378381257465 27.688456358476067 27.8930438355539 22.70412142471257 86-87 21.81921494971589 27.686693136159384 28.00784574784242 22.486246166282303 88-89 21.90572243596419 27.515034453113003 27.78127277992471 22.7979703309981 90-91 21.760794498688103 27.31407823449808 28.149669386827398 22.77545787998642 92-93 21.91038887729058 27.456240426441543 28.15987468119842 22.473496015069454 94-95 21.53223424951127 27.33773264862906 28.158074813721797 22.971958288137877 96-97 21.483528345473445 27.14986499891105 28.309714086809645 23.056892568805857 98-99 21.426947653570387 27.419146196004725 27.92806717509621 23.225838975328674 100-101 21.37156578182541 27.343031292423813 28.116375509085774 23.169027416665003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 92.5 2 179.0 3 254.0 4 201.5 5 197.0 6 274.0 7 497.0 8 785.0 9 962.0 10 922.0 11 769.5 12 809.0 13 928.0 14 1014.0 15 1080.0 16 1138.5 17 1189.5 18 1297.0 19 1454.0 20 1559.0 21 1786.5 22 2182.0 23 2681.5 24 3212.0 25 3913.0 26 5127.0 27 6676.0 28 8052.5 29 9835.5 30 12255.0 31 14923.0 32 18061.0 33 20809.5 34 23541.5 35 26145.0 36 29082.5 37 32032.0 38 33045.0 39 33437.5 40 34040.0 41 35032.0 42 35723.0 43 36972.5 44 38654.0 45 40044.5 46 40599.5 47 41857.5 48 47529.5 49 47038.0 50 40004.5 51 35935.5 52 33304.0 53 31807.5 54 30617.5 55 28224.0 56 25275.0 57 22431.0 58 20035.0 59 17583.5 60 14702.5 61 12139.5 62 9902.0 63 7499.0 64 5351.5 65 4086.0 66 3114.5 67 2389.0 68 1770.0 69 1246.0 70 998.5 71 770.5 72 593.0 73 511.0 74 391.0 75 307.0 76 230.0 77 145.0 78 87.5 79 56.0 80 43.5 81 27.0 82 15.0 83 12.5 84 6.5 85 1.5 86 3.0 87 3.0 88 3.0 89 2.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035851053612064976 2 0.024154290762505314 3 0.024249386395428563 4 0.05705737975394956 5 0.060766109437956274 6 0.05239769374071034 7 0.05144673741147786 8 0.0449802343726969 9 0.046977242664085136 10-11 0.05534565836133107 12-13 0.04811839025916413 14-15 0.04222246101792267 16-17 0.050353137632860485 18-19 0.05477508456379158 20-21 0.011601667216636411 22-23 0.021158778325422962 24-25 0.005800833608318206 26-27 0.01036542398863417 28-29 0.005467998893086833 30-31 0.009462015475863303 32-33 0.0036136340510834718 34-35 0.007369911551551819 36-37 0.008368415697245935 38-39 0.011221284684943414 40-41 0.0018068170255417359 42-43 0.0019494604749266098 44-45 0.002187199557234733 46-47 2.852868987697478E-4 48-49 0.0012837910444638652 50-51 3.8038253169299705E-4 52-53 0.0035660862346218476 54-55 0.007179720285705319 56-57 0.016641735761568622 58-59 0.010127684906326047 60-61 0.009889945824017922 62-63 0.005420451076625208 64-65 4.754781646162463E-5 66-67 0.0072272681021669435 68-69 0.01778288335664761 70-71 0.007655198450321567 72-73 0.0031857037029288508 74-75 0.005467998893086833 76-77 0.008748798228938932 78-79 0.007037076836320446 80-81 0.01169676284955966 82-83 0.006941981203397196 84-85 0.01797307462249411 86-87 0.004564590380315965 88-89 0.006799337754012323 90-91 0.00399401658277647 92-93 0.016594187945107 94-95 0.011554119400174787 96-97 0.009509563292324927 98-99 0.02196709120527058 100-101 0.04032054835945769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1051573.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.91733739955103 #Duplication Level Percentage of deduplicated Percentage of total 1 85.82558276983676 58.290450624920965 2 8.903536947987131 12.094090458916215 3 2.2616611559883104 4.6081801144415 4 0.9138993284582722 2.482784361604943 5 0.4848576304228407 1.646511963808744 6 0.29762461833675574 1.2128322973194023 7 0.20542106384885228 0.9766156191678051 8 0.15964839066623568 0.867431489133925 9 0.12244350889957427 0.7484433395685584 >10 0.7405005122228379 9.448198054544358 >50 0.05553396865364183 2.565059004112832 >100 0.02718794544917988 3.200211915572814 >500 9.810076404711246E-4 0.4682956291347442 >1k 0.0011211515891098567 1.3908951277532553 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3294 0.3132450148491831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2637 0.2507671840186083 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2259 0.21482103477362008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1623 0.15434021223443356 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1487 0.14140720615687166 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1201 0.11420985514082235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1074 0.10213270975956971 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.509563292324926E-5 0.0 4 0.0 0.0 0.0 9.509563292324926E-5 0.0 5 0.0 0.0 0.0 9.509563292324926E-5 0.0 6 0.0 0.0 0.0 9.509563292324926E-5 0.0 7 0.0 0.0 0.0 9.509563292324926E-5 0.0 8 0.0 0.0 0.0 9.509563292324926E-5 0.0 9 0.0 0.0 0.0 9.509563292324926E-5 0.0 10-11 0.0 0.0 0.0 9.509563292324926E-5 0.0 12-13 0.0 0.0 0.0 9.509563292324926E-5 0.0 14-15 0.0 0.0 0.0 9.509563292324926E-5 0.0 16-17 0.0 0.0 0.0 9.509563292324926E-5 0.0 18-19 0.0 0.0 0.0 9.509563292324926E-5 0.0 20-21 0.0 0.0 0.0 9.509563292324926E-5 0.0 22-23 0.0 0.0 0.0 1.9019126584649852E-4 0.0 24-25 0.0 0.0 0.0 4.7547816461624635E-4 0.0 26-27 0.0 0.0 0.0 0.0012362432280022405 0.0 28-29 0.0 0.0 0.0 0.0023773908230812317 0.0 30-31 0.0 0.0 0.0 0.004802329462624088 0.0 32-33 0.0 0.0 0.0 0.007702746266783191 0.0 34-35 0.0 0.0 0.0 0.010508067438019044 0.0 36-37 0.0 0.0 0.0 0.014549631837257137 0.0 38-39 0.0 0.0 0.0 0.02168180430650083 0.0 40-41 0.0 0.0 0.0 0.03171439357990363 0.0 42-43 0.0 0.0 0.0 0.041033765606382056 0.0 44-45 0.0 0.0 0.0 0.04607383415131427 0.0 46-47 0.0 0.0 0.0 0.05244524155717197 0.0 48-49 0.0 0.0 0.0 0.06276311772934451 0.0 50-51 0.0 0.0 0.0 0.07279570700274732 0.0 52-53 0.0 0.0 0.0 0.08558606963092433 0.0 54-55 0.0 0.0 0.0 0.09523827637263414 0.0 56-57 0.0 0.0 0.0 0.12338658371791592 0.0 58-59 0.0 0.0 0.0 0.17459558204708564 0.0 60-61 0.0 0.0 0.0 0.21187307015299936 0.0 62-63 0.0 0.0 0.0 0.2326039181302677 0.0 64-65 0.0 0.0 0.0 0.2750641182304985 0.0 66-67 0.0 0.0 0.0 0.3678299081471281 0.0 68-69 0.0 0.0 0.0 0.49221499601073826 0.0 70-71 0.0 0.0 0.0 0.5962020706123112 0.0 72-73 0.0 0.0 0.0 0.7219660451533084 0.0 74-75 0.0 0.0 0.0 0.8710759975769633 0.0 76-77 0.0 0.0 0.0 0.9652682219874417 0.0 78-79 0.0 0.0 0.0 1.0661171407025476 0.0 80-81 0.0 0.0 0.0 1.192594332490469 0.0 82-83 0.0 0.0 0.0 1.3182156635820812 0.0 84-85 0.0 0.0 0.0 1.4880089161665428 0.0 86-87 0.0 0.0 0.0 1.7845646474376957 0.0 88-89 0.0 0.0 0.0 2.1651849182130007 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1375 0.0 39.404667 2 GTACCTG 1385 0.0 38.775154 1 TACGCCG 50 0.0016111553 38.02205 5 CGCACCG 50 0.0016111553 38.02205 5 TATCACG 75 3.0678153E-4 31.68504 2 ACCTGGG 1885 0.0 27.987051 3 GTACATG 4725 0.0 26.050903 1 TACATGG 4855 0.0 25.550346 2 TAACGCA 95 0.0012216663 25.016886 4 ACATGGG 4900 0.0 24.054764 3 GAGTACT 3350 0.0 22.48692 12-13 AGTACTT 3510 0.0 20.784845 12-13 GTACTTT 3705 0.0 20.524723 14-15 GTATCAA 8175 0.0 20.405357 1 CATGGGA 3100 0.0 20.392797 4 CATGGGG 1845 0.0 20.352493 4 ATCACGC 125 0.0060106367 19.011023 3 ATGGGAT 1210 0.0 18.853909 5 CGACGAG 90 1.232279E-4 18.475466 94-95 GTACTGG 645 0.0 18.420658 1 >>END_MODULE