FastQCFastQC Report
Thu 26 May 2016
SRR936529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051573
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.4049172049871954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.3640260828301982No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28380.2698814062361814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.21377498281146437No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14120.13427503368762797No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13520.12856929571223302No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.10612672634234617No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10930.10393952678511144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12300.040.1592252
GTACCTG13650.036.897381
ACCTGGG18200.025.3126013
GTACATG44450.024.157891
GGGCGCG1601.862205E-623.7457457
TACATGG46250.022.9007762
GAGTACT48100.022.07459812-13
ACATGGG46200.021.6908663
TACGAGA1100.002880198921.587044
TCGCGCT1259.484211E-920.90321260-61
CGATCGT1259.509677E-920.89824324-25
TCTATAC3700.020.5378383
CCCGCGA1301.4941179E-820.0935120-21
GTACTTT52550.020.06968714-15
GTACACG2151.0755521E-619.8896141
CTCGCGC1352.2973836E-819.35482660-61
AGTACTT50600.019.34091612-13
GTATCAA98950.019.0152361
GTACCGG1507.705081E-419.0056321
CGCGCTT1252.175002E-718.99930662-63
Produced by FastQC (version 0.11.5)