##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1051573 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.011377241522936 31.0 31.0 34.0 30.0 34.0 2 32.16351313698621 33.0 31.0 34.0 30.0 34.0 3 32.269224295412684 34.0 31.0 34.0 30.0 34.0 4 35.98929223173284 37.0 35.0 37.0 35.0 37.0 5 35.79562141667768 37.0 35.0 37.0 35.0 37.0 6 35.89973306655838 37.0 35.0 37.0 35.0 37.0 7 35.855786521715565 37.0 35.0 37.0 35.0 37.0 8 35.82850263367355 37.0 35.0 37.0 35.0 37.0 9 37.48850151154509 39.0 37.0 39.0 35.0 39.0 10-11 37.36597411687063 39.0 37.0 39.0 34.0 39.0 12-13 37.28244449030167 39.0 37.0 39.0 34.0 39.0 14-15 38.417859245149884 40.0 38.0 41.0 34.0 41.0 16-17 38.34859206160675 40.0 38.0 41.0 33.5 41.0 18-19 38.50026912064118 40.0 38.0 41.0 34.0 41.0 20-21 38.534590085519504 40.0 38.0 41.0 34.0 41.0 22-23 38.4430400932698 40.0 38.0 41.0 34.0 41.0 24-25 38.33191228759202 40.0 38.0 41.0 34.0 41.0 26-27 38.1277971191729 40.0 37.5 41.0 33.0 41.0 28-29 38.048499723747184 40.0 37.0 41.0 33.0 41.0 30-31 38.0086498987707 40.0 37.0 41.0 33.0 41.0 32-33 37.86045048703228 40.0 37.0 41.0 33.0 41.0 34-35 37.690021520141734 40.0 37.0 41.0 33.0 41.0 36-37 37.43529407849003 40.0 36.5 41.0 32.5 41.0 38-39 37.27602696151385 40.0 36.0 41.0 32.0 41.0 40-41 37.06720503474319 39.0 36.0 41.0 31.5 41.0 42-43 36.82393614138058 39.0 36.0 41.0 31.0 41.0 44-45 36.570783483410096 39.0 35.5 41.0 30.5 41.0 46-47 36.20234163486511 39.0 35.0 40.0 30.0 41.0 48-49 35.84275176331077 39.0 35.0 40.0 29.0 41.0 50-51 35.5386806241697 39.0 35.0 40.0 28.0 41.0 52-53 35.27603124081733 38.0 35.0 40.0 27.0 41.0 54-55 35.03164497376787 38.0 34.0 40.0 26.5 41.0 56-57 34.72590680818165 38.0 34.0 40.0 26.0 41.0 58-59 34.4042520110349 38.0 34.0 40.0 26.0 41.0 60-61 34.0565043986485 37.0 33.0 40.0 25.0 41.0 62-63 33.67703098120625 37.0 33.0 39.5 23.5 41.0 64-65 33.270405858651756 36.0 32.5 39.0 22.5 40.0 66-67 32.819049652282814 36.0 32.0 39.0 22.0 40.0 68-69 32.54188629795554 35.0 31.5 38.5 21.5 40.0 70-71 32.10981215759629 35.0 31.0 38.0 20.5 40.0 72-73 31.66248515319431 35.0 31.0 37.0 19.0 39.0 74-75 31.13759910153646 34.5 30.5 37.0 18.0 39.0 76-77 29.716712962390627 33.0 28.5 35.0 16.0 37.5 78-79 30.471677192168304 34.0 30.0 36.0 16.0 38.0 80-81 30.267184969564642 34.0 30.0 35.5 11.5 37.0 82-83 29.923869764628797 34.0 30.0 35.0 7.5 37.0 84-85 29.497940228590885 34.0 29.0 35.0 7.0 36.0 86-87 29.11678029009874 34.0 29.0 35.0 2.0 36.0 88-89 28.69971889730908 34.0 29.0 35.0 2.0 35.5 90-91 28.222735844301823 33.0 28.5 35.0 2.0 35.0 92-93 27.751749521906703 33.0 27.0 35.0 2.0 35.0 94-95 27.178601961062142 33.0 26.0 34.5 2.0 35.0 96-97 26.43745084744473 32.0 25.0 34.0 2.0 35.0 98-99 25.51954025065307 32.0 23.5 34.0 2.0 35.0 100-101 22.300566865067857 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25993388969634523 1101 2 0.29003153585730956 1101 3 0.2813158110963059 1101 4 0.14990921769729937 1101 5 0.05415405128132278 1101 6 0.08351604279238245 1101 7 0.05674539488423136 1101 8 0.24400326401951133 1101 9 0.19392882009972823 1101 10-11 0.2793594119354097 1101 12-13 0.22766385186496763 1101 14-15 0.34895355240663406 1101 16-17 0.4050062472978624 1101 18-19 0.21321462008121017 1101 20-21 0.21473600749856558 1101 22-23 0.09090651877400546 1101 24-25 0.08993049082520344 1101 26-27 0.08114786083920222 1101 28-29 0.1422444788275854 1101 30-31 0.1519913578355272 1101 32-33 0.3674600849112011 1101 34-35 0.21496394980580646 1101 36-37 0.20561672043428558 1101 38-39 0.2860536077412874 1101 40-41 0.31129631992026674 1101 42-43 0.31072884160720093 1101 44-45 0.31472470102922756 1101 46-47 0.32274851564589824 1101 48-49 0.3911610699052588 1101 50-51 0.48293505513733237 1101 52-53 0.5290045623047988 1101 54-55 0.5591506862895557 1101 56-57 0.4347720284299612 1101 58-59 0.507371881678047 1101 60-61 0.5165242468088138 1101 62-63 0.4352278375133949 1101 64-65 0.5226984426580046 1101 66-67 0.4493215472749341 1101 68-69 0.3234961827856466 1101 70-71 0.49209979141402727 1101 72-73 0.33608351834247685 1101 74-75 0.5697887571467959 1101 76-77 0.3275498521380875 1101 78-79 0.26925014740796627 1101 80-81 0.2420487671745839 1101 82-83 0.35354104078362525 1101 84-85 0.27616630345746884 1101 86-87 0.07140470137765575 1101 88-89 -0.3648701792814535 1101 90-91 -0.2323928404005393 1101 92-93 -0.13543858172571532 1101 94-95 0.12385618782967711 1101 96-97 0.3143754060117878 1101 98-99 0.5111617986983816 1101 100-101 0.8697353068464935 1102 1 0.17017224382741247 1102 2 0.20099479031458856 1102 3 0.21518418990850563 1102 4 0.12382143571970516 1102 5 -0.05428413370633223 1102 6 0.07195906044889 1102 7 0.048935456845946135 1102 8 -0.011201347727329392 1102 9 0.1790234886304205 1102 10-11 0.1499716075204205 1102 12-13 0.17725393799003797 1102 14-15 0.27881944584628826 1102 16-17 0.3115804028752862 1102 18-19 0.2197231894386391 1102 20-21 0.15205456667287365 1102 22-23 0.06979983760933095 1102 24-25 0.20424640929117288 1102 26-27 -0.1541392514685711 1102 28-29 -0.05249740487769117 1102 30-31 0.028818710741163045 1102 32-33 0.045428292854033714 1102 34-35 0.06653456717924655 1102 36-37 0.1592524407608309 1102 38-39 0.2431619458209937 1102 40-41 0.2836991919063223 1102 42-43 0.21480295403578964 1102 44-45 0.33804923200145254 1102 46-47 0.22139277944057767 1102 48-49 0.3423148695911351 1102 50-51 0.3047769923471577 1102 52-53 0.3659382339249433 1102 54-55 0.4036716479906559 1102 56-57 0.20729571713705042 1102 58-59 0.09575535395061507 1102 60-61 0.31226114250946324 1102 62-63 0.14657511654824162 1102 64-65 0.14544736825216376 1102 66-67 0.20006198117955876 1102 68-69 0.10181130731317012 1102 70-71 0.03345276609429959 1102 72-73 -0.055362657808299076 1102 74-75 -0.0597612868650117 1102 76-77 -0.19415918667860055 1102 78-79 -0.02289072488876087 1102 80-81 0.26130781548401316 1102 82-83 0.0696536764866913 1102 84-85 -0.12339012658101822 1102 86-87 0.15635784174942913 1102 88-89 0.012090072017322484 1102 90-91 0.14596198104960934 1102 92-93 0.3041936176563951 1102 94-95 0.46656489871526574 1102 96-97 0.7156595151610681 1102 98-99 0.5713570040005074 1102 100-101 1.0180464616817488 1103 1 0.24819841673258125 1103 2 0.22567787876172218 1103 3 0.2508494825530505 1103 4 0.15597521538860804 1103 5 -0.15705378554685723 1103 6 0.16589840402090061 1103 7 0.0481764204343591 1103 8 0.05866823097976237 1103 9 0.03403257490571576 1103 10-11 -0.10778053661893239 1103 12-13 0.09401534569785497 1103 14-15 0.072282591027232 1103 16-17 0.20756194222329327 1103 18-19 0.17026919701791599 1103 20-21 0.22905853360769157 1103 22-23 0.11881419835662399 1103 24-25 0.07215856052225433 1103 26-27 0.2240805588277297 1103 28-29 0.1688376127408162 1103 30-31 0.07416559592167005 1103 32-33 0.06774424037525506 1103 34-35 -0.0464369218618188 1103 36-37 -0.12030482493515393 1103 38-39 -0.013984908333206647 1103 40-41 -0.02316011487736347 1103 42-43 0.03639781696600153 1103 44-45 -0.023642421922268397 1103 46-47 0.1606094760254635 1103 48-49 -0.09212931630076326 1103 50-51 0.04876232133680958 1103 52-53 0.00188027013707881 1103 54-55 -0.007131046865623603 1103 56-57 0.14940256951831543 1103 58-59 0.14757039284430817 1103 60-61 0.07251173533352073 1103 62-63 -0.08978715989614017 1103 64-65 -0.1741033597579431 1103 66-67 0.1371896655090552 1103 68-69 0.20471669924189229 1103 70-71 0.15890791092066792 1103 72-73 0.22339900475845909 1103 74-75 0.19792741955965454 1103 76-77 0.0673947880127983 1103 78-79 -0.07181366884195484 1103 80-81 0.19034178928894718 1103 82-83 0.16615352135516304 1103 84-85 0.1707805291821174 1103 86-87 0.06108602914831707 1103 88-89 0.0513391800110341 1103 90-91 0.18416589036414877 1103 92-93 0.35815002569664145 1103 94-95 0.6027047276273478 1103 96-97 0.22375128654151766 1103 98-99 0.5609316111853104 1103 100-101 1.2531912326411287 1104 1 0.047090250871018924 1104 2 0.17901346819142105 1104 3 0.1691359735912954 1104 4 0.1678282099603976 1104 5 0.04340018782365718 1104 6 0.09058421053384791 1104 7 0.04415279706814346 1104 8 -0.003324430293396574 1104 9 0.07509334010720181 1104 10-11 0.16027653431907396 1104 12-13 0.04712117548104544 1104 14-15 -0.023392211922747208 1104 16-17 0.17310535208281408 1104 18-19 -0.0690778215394161 1104 20-21 -0.13351463861471302 1104 22-23 0.10274517086242696 1104 24-25 0.05974474868521895 1104 26-27 0.11946040924627255 1104 28-29 0.02881325595463835 1104 30-31 -0.019142569487172523 1104 32-33 0.1123173288429058 1104 34-35 0.06129799888807952 1104 36-37 0.09695980158658557 1104 38-39 0.2767841300264635 1104 40-41 0.039897306925134046 1104 42-43 0.06968863156015459 1104 44-45 0.09644066505138227 1104 46-47 0.20685624467675012 1104 48-49 0.14445803990138728 1104 50-51 -0.014310663003314517 1104 52-53 0.13128673192907314 1104 54-55 0.20057280540005706 1104 56-57 0.3854361823359653 1104 58-59 0.281361811830827 1104 60-61 0.38019640323462056 1104 62-63 0.5232676021271914 1104 64-65 0.48650287748078114 1104 66-67 0.2633229012039706 1104 68-69 0.517712719759551 1104 70-71 0.06107642320997542 1104 72-73 0.13884943552950446 1104 74-75 0.2076421214606583 1104 76-77 0.12703495950630384 1104 78-79 0.18006741752503785 1104 80-81 0.5199823682379794 1104 82-83 0.3503774657057903 1104 84-85 0.17578515832967412 1104 86-87 0.08234954874918898 1104 88-89 0.20477137035614845 1104 90-91 0.199756157609702 1104 92-93 0.07740241270695947 1104 94-95 0.2028847437444803 1104 96-97 0.5102802407842582 1104 98-99 0.45184954058684923 1104 100-101 1.0563406941207347 1105 1 0.05769442005449932 1105 2 0.15938233220301612 1105 3 0.14267375140160965 1105 4 0.06264192924155765 1105 5 -0.013684165962665418 1105 6 0.09764226744492532 1105 7 0.05180268620637207 1105 8 0.033054337450892035 1105 9 -0.0709415670117508 1105 10-11 -0.04069108086550699 1105 12-13 -0.08397484899274588 1105 14-15 -0.02696608539755374 1105 16-17 0.1582015522758553 1105 18-19 -0.014712235180112998 1105 20-21 0.0011558694702102912 1105 22-23 -0.003385295806431543 1105 24-25 -0.004370510946088757 1105 26-27 0.08942919467231292 1105 28-29 0.03677736529595421 1105 30-31 0.035924063499976455 1105 32-33 -0.06250228435507665 1105 34-35 0.004579116755436985 1105 36-37 -0.09666390782257395 1105 38-39 0.1258510485574007 1105 40-41 0.11950819859054462 1105 42-43 -0.024056067880593446 1105 44-45 0.11995074267291983 1105 46-47 0.1279506705933855 1105 48-49 0.007614226256549728 1105 50-51 0.11414835230988274 1105 52-53 -0.001182709393511061 1105 54-55 0.044037757339616235 1105 56-57 -0.13849214373064456 1105 58-59 -0.25164442742315174 1105 60-61 -0.4462088322230784 1105 62-63 -0.14639300344530426 1105 64-65 -0.18818433613648722 1105 66-67 0.2268831198280168 1105 68-69 0.16394775159036357 1105 70-71 0.09603631894987785 1105 72-73 -0.023218328650372655 1105 74-75 -0.1639223534743337 1105 76-77 -0.08815950924190474 1105 78-79 0.07442841565924141 1105 80-81 0.15075013937340742 1105 82-83 0.21031457285426924 1105 84-85 0.10192571665321637 1105 86-87 0.1317498457635473 1105 88-89 0.2592873553175039 1105 90-91 0.27912238461214045 1105 92-93 0.5248815735010623 1105 94-95 0.5051264455855033 1105 96-97 0.5286041597142237 1105 98-99 1.0264750084088732 1105 100-101 1.0246993298071203 1106 1 -0.09776366961411753 1106 2 0.0012217793056166215 1106 3 -0.013537483756991264 1106 4 0.00783951917598813 1106 5 -0.13064323029015412 1106 6 -0.060784101507756816 1106 7 -0.0428721321526524 1106 8 -0.17698464890388976 1106 9 0.007607077326369449 1106 10-11 -0.020201093270259207 1106 12-13 0.01579477709267252 1106 14-15 0.11987513381208004 1106 16-17 0.02660045356950036 1106 18-19 -0.16068955221005865 1106 20-21 -0.09542396395173114 1106 22-23 0.028291117448922876 1106 24-25 -0.18951139345539048 1106 26-27 -0.1767634333624173 1106 28-29 -0.059603106084516355 1106 30-31 -0.07500983690761132 1106 32-33 -0.11178901127338037 1106 34-35 -0.030419997191046377 1106 36-37 -0.17129176534516688 1106 38-39 0.15863502870980284 1106 40-41 0.11826772366585914 1106 42-43 0.06729604978731629 1106 44-45 0.05586692201015353 1106 46-47 -0.21820828740767695 1106 48-49 -0.2406136667081924 1106 50-51 -0.1713380910712914 1106 52-53 -0.35522754370150267 1106 54-55 -0.3977706778898664 1106 56-57 -0.2702039991267071 1106 58-59 -0.3060038107298979 1106 60-61 -0.32134174025072326 1106 62-63 -0.4748308910937169 1106 64-65 -0.21146539231228445 1106 66-67 -0.4445460844959257 1106 68-69 -0.1976949916313302 1106 70-71 -0.2655399702579899 1106 72-73 -0.38016498822861067 1106 74-75 -0.2998872657550322 1106 76-77 -0.7194504892170919 1106 78-79 -0.5676945685690065 1106 80-81 -0.5802661640113165 1106 82-83 -0.2404208515653643 1106 84-85 -0.20125964574267385 1106 86-87 -0.028891656983216052 1106 88-89 -0.05450849795203183 1106 90-91 0.15669193981327467 1106 92-93 -0.23163091985353645 1106 94-95 -0.24644629940829432 1106 96-97 -0.4783735117371286 1106 98-99 0.08648298289053002 1106 100-101 0.004275796225691408 1107 1 0.030730177604343822 1107 2 0.08761782752132774 1107 3 0.07107249362309176 1107 4 0.04530666285759111 1107 5 -0.12327466240167695 1107 6 0.09133005385578485 1107 7 0.07162034200676004 1107 8 0.003266960064216562 1107 9 0.06810851739726331 1107 10-11 -0.058896673497109475 1107 12-13 0.008787936414016428 1107 14-15 0.15270170715449893 1107 16-17 0.033390873337168614 1107 18-19 -0.07429282020000016 1107 20-21 0.12986439212460255 1107 22-23 -0.02373058891884483 1107 24-25 0.05762282443430422 1107 26-27 0.033740947024170964 1107 28-29 0.037626637875526114 1107 30-31 0.04885814155013435 1107 32-33 0.12805835215237238 1107 34-35 0.19030745058757503 1107 36-37 0.05517579408340367 1107 38-39 0.06845486257513045 1107 40-41 0.06710691438157568 1107 42-43 -0.0676938437322292 1107 44-45 0.023921075787292523 1107 46-47 0.21071847001217492 1107 48-49 0.15790373038760208 1107 50-51 0.24335924787462204 1107 52-53 0.20220058267462804 1107 54-55 0.22226223997001426 1107 56-57 0.024815653122836068 1107 58-59 0.22297812889379287 1107 60-61 0.29521066438047683 1107 62-63 0.30170614184054756 1107 64-65 0.38627924879141773 1107 66-67 0.547715756770792 1107 68-69 0.4880433831809654 1107 70-71 0.4177172567644689 1107 72-73 0.5797441491784916 1107 74-75 0.3477938508537832 1107 76-77 0.05950444052987436 1107 78-79 0.41797310190776216 1107 80-81 0.6809757569584676 1107 82-83 0.664356682781623 1107 84-85 0.3630987363451972 1107 86-87 0.3336875605919367 1107 88-89 0.5900401567509697 1107 90-91 0.6229880583230418 1107 92-93 0.6062339186205641 1107 94-95 0.8201822799132152 1107 96-97 0.9508691586712601 1107 98-99 1.0065313545026733 1107 100-101 1.3048417044276661 1108 1 -0.008535167755859163 1108 2 0.0351335614872994 1108 3 0.11390767136982305 1108 4 -0.07073748500655341 1108 5 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-0.6219246289076565 2316 86-87 -0.6193798724714306 2316 88-89 -0.7798872858308172 2316 90-91 -0.7418770837557496 2316 92-93 -0.684282435005052 2316 94-95 -0.8150489930109863 2316 96-97 -0.7454189662825819 2316 98-99 -0.4943250979175495 2316 100-101 -0.7352023182524228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 10.0 5 34.0 6 59.0 7 152.0 8 313.0 9 646.0 10 1147.0 11 1969.0 12 2952.0 13 4063.0 14 5009.0 15 6779.0 16 8672.0 17 11405.0 18 9838.0 19 6299.0 20 5630.0 21 5702.0 22 6018.0 23 6972.0 24 8085.0 25 9444.0 26 11820.0 27 14734.0 28 18215.0 29 22788.0 30 28657.0 31 35994.0 32 45782.0 33 59038.0 34 79314.0 35 109173.0 36 157259.0 37 217415.0 38 141719.0 39 8462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06107451185578 19.69919375936452 14.586914643137442 21.652817085642255 2 15.665456661889415 21.26615888944576 40.01470265170787 23.05368179695695 3 19.818358186244676 25.840307364576994 30.107938983566644 24.233395465611686 4 12.861082582068544 17.3494617520636 37.761991707558295 32.02746395830956 5 13.2255131869992 38.017775394031375 34.14992496904616 14.606786449923256 6 30.742794107857772 36.87733579314739 18.062706239242278 14.31716385975256 7 28.47534122690484 33.07730419095964 20.966875338183844 17.48047924395168 8 26.56696206540107 34.44763226138366 19.897049467797288 19.088356205417977 9 26.736854718151005 17.136129697324726 20.6569122329196 35.47010335160467 10-11 24.687965674084484 27.041405825781904 27.51883617496555 20.751792325168058 12-13 26.312613758908142 24.364329010257084 28.084067001025137 21.238990229809637 14-15 22.88906735669994 25.77144930285813 27.024844906558787 24.314638433883143 16-17 21.64181340007998 29.089472235205843 27.57098289791577 21.69773146679841 18-19 21.3740748383612 27.528569105520965 30.312446211532627 20.784909844585204 20-21 22.28841934891824 26.427789606617896 30.314680958906326 20.969110085557542 22-23 21.889038719763853 26.4795693239352 30.20471567848027 21.42667627782068 24-25 22.123775986366933 26.47264400258283 30.442218758588425 20.961361252461817 26-27 21.84003857287954 26.574013139124848 30.529777958680608 21.05617032931501 28-29 21.37008747900043 26.954630462206953 30.620883596391664 21.054398462400957 30-31 21.597820375966126 26.659930626713233 30.43799316727806 21.304255830042578 32-33 21.5463786645923 26.790364660722837 30.256003781025736 21.407252893659127 34-35 22.116470099280715 26.651722409633056 30.099585054348083 21.132222436738147 36-37 22.284300722861317 26.397491455818244 30.09539715049696 21.22281067082348 38-39 21.840765027842107 26.71948186577775 30.38479645066645 21.054956655713696 40-41 21.953889196370323 26.713368975820543 30.19312370073244 21.13961812707669 42-43 21.784826323064348 26.98222505609499 29.64554242749731 21.587406193343348 44-45 22.018520059435364 26.872534918276376 29.460635958395244 21.648309063893016 46-47 22.41909140834322 27.26958911534868 28.844563736689576 21.466755739618524 48-49 22.479674352192042 27.35217159558772 28.564602014805544 21.60355203741469 50-51 22.246265796723545 27.589502483446072 28.668753084693925 21.495478635136458 52-53 22.512010818295245 27.79710370956453 28.281435440187458 21.40945003195277 54-55 22.12472991889959 27.51765037050562 28.467926918336605 21.889692792258185 56-57 22.432603478747932 27.811772358403946 28.088932929424143 21.666691233423975 58-59 22.688743592291683 27.345467305650477 28.088264592172337 21.8775245098855 60-61 22.268319183283367 27.506365739364874 28.753598931078834 21.471716146272925 62-63 22.341478213195284 27.485985756802418 28.65491319942236 21.51762283057994 64-65 22.207977722786403 27.69377961307797 28.375439143757642 21.72280352037798 66-67 22.16794725062373 27.712252095382144 28.1511328401195 21.968667813874625 68-69 22.18703045057725 27.67588489263366 28.33586929646043 21.80121536032866 70-71 22.340848910416458 27.661314518318537 28.07678793120582 21.921048640059187 72-73 22.34498157737952 27.46494376690642 28.21830032603925 21.971774329674805 74-75 22.13007414113825 28.069591481947832 27.870734221708247 21.929600155205666 76-77 22.34013315619749 27.72034916175882 28.04753377642654 21.891983905617153 78-79 22.327126862868354 27.675678554344735 27.98451219500598 22.012682387780934 80-81 22.37966603631024 28.083924104262252 27.679462569238954 21.85694729018855 82-83 22.432287597673664 28.036531721324607 27.736728911214147 21.794451769787578 84-85 22.185972885749923 27.94020238446772 27.698503157591414 22.175321572190942 86-87 22.21345196636533 27.879507462431075 27.720644315828114 22.186396255375477 88-89 22.364288353456814 27.838452294961368 27.42745489276168 22.369804458820134 90-91 22.21548007521193 27.70962185749109 27.695403113286776 22.37949495401021 92-93 22.323505413851013 27.722473805633907 27.800548136961968 22.153472643553116 94-95 22.07706063961862 27.61945835118399 27.750883577885375 22.552597431312012 96-97 22.03582763744428 27.550430906389302 27.88003566121843 22.533705794947995 98-99 21.995273576248056 27.80111931604749 27.422767668218366 22.780839439486083 100-101 21.63787348243784 28.24096880521758 27.32967070589108 22.791487006453504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 270.0 1 205.0 2 291.5 3 396.5 4 277.5 5 227.5 6 331.5 7 580.5 8 861.5 9 1048.0 10 1046.0 11 957.5 12 981.5 13 1117.0 14 1236.0 15 1329.5 16 1449.0 17 1546.0 18 1655.0 19 1787.5 20 1961.0 21 2247.0 22 2583.5 23 2963.5 24 3516.5 25 4288.0 26 5420.0 27 6957.5 28 8488.0 29 10224.5 30 12484.0 31 15154.5 32 18389.5 33 21229.5 34 23764.0 35 26293.5 36 29293.5 37 31683.0 38 32548.0 39 33332.0 40 34022.0 41 34978.0 42 35775.0 43 36680.0 44 38102.5 45 39267.5 46 39550.0 47 41334.0 48 47843.5 49 47031.0 50 39705.0 51 35887.5 52 32898.5 53 31333.0 54 29845.5 55 27431.0 56 24659.0 57 21914.5 58 19867.5 59 17627.0 60 14683.0 61 11986.5 62 9819.0 63 7475.5 64 5281.5 65 3943.0 66 2998.0 67 2342.5 68 1748.0 69 1202.0 70 879.5 71 680.5 72 549.5 73 480.0 74 404.5 75 338.5 76 242.5 77 148.0 78 108.0 79 75.0 80 48.0 81 37.5 82 29.5 83 18.0 84 10.5 85 5.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03784806190345321 2 0.005895929241241454 3 0.005040068544932211 4 0.0012362432280022403 5 6.656694304627449E-4 6 2.852868987697478E-4 7 0.0 8 0.0 9 0.0016166257596952375 10-11 5.705737975394956E-4 12-13 6.656694304627449E-4 14-15 0.0028053211712358536 16-17 0.0032807993358520996 18-19 0.0 20-21 0.0 22-23 0.0016166257596952375 24-25 0.0011411475950789912 26-27 0.005420451076625208 28-29 0.0060385726906263286 30-31 0.0014739823103103635 32-33 6.181216140011201E-4 34-35 0.010460519621557418 36-37 0.012029597564791033 38-39 0.010270328355710921 40-41 0.0018068170255417359 42-43 8.083128798476187E-4 44-45 9.985041456941175E-4 46-47 4.754781646162463E-5 48-49 7.132172469243694E-4 50-51 9.509563292324926E-4 52-53 0.001997008291388235 54-55 0.003518538418160223 56-57 0.0015690779432336128 58-59 7.132172469243694E-4 60-61 0.004326851298007842 62-63 0.0050876163613938355 64-65 0.009034085127708681 66-67 0.0032807993358520996 68-69 0.0031381558864672257 70-71 1.426434493848739E-4 72-73 0.0011886954115406159 74-75 0.006181216140011202 76-77 0.0024724864560044806 78-79 0.0034234427852369736 80-81 0.004897425095547337 82-83 0.004612138196777589 84-85 0.005658190158933331 86-87 0.0037087296840067215 88-89 0.010127684906326047 90-91 0.013693771140947894 92-93 0.001046051962155742 94-95 0.002329843006619607 96-97 9.985041456941175E-4 98-99 0.0036136340510834718 100-101 0.004849877279085713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1051573.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.08491467156057 #Duplication Level Percentage of deduplicated Percentage of total 1 84.32905650566255 54.88549447004256 2 9.847038736548985 12.817873518716846 3 2.479975691884816 4.842270188816029 4 1.0453348225361587 2.721421109119072 5 0.5303462878760691 1.7258771446396426 6 0.32905117909353876 1.2849760748327619 7 0.23373696861827975 1.0648925460676977 8 0.1706739484755136 0.88866394985497 9 0.12296935414392915 0.7203104929507088 >10 0.8129206271612008 9.913591093265628 >50 0.06242723645660459 2.7421328049033 >100 0.03368589484620463 3.9338151104253845 >500 0.0014646035243235675 0.6310422016027227 >1k 0.0013181431718912108 1.8276392947626892 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4258 0.4049172049871954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3828 0.3640260828301982 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2838 0.2698814062361814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2248 0.21377498281146437 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1412 0.13427503368762797 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1352 0.12856929571223302 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.10612672634234617 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1093 0.10393952678511144 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.509563292324926E-5 0.0 24-25 0.0 4.754781646162463E-5 0.0 3.8038253169299705E-4 0.0 26-27 0.0 9.509563292324926E-5 0.0 0.0011886954115406159 0.0 28-29 0.0 9.509563292324926E-5 0.0 0.002472486456004481 0.0 30-31 0.0 9.509563292324926E-5 0.0 0.004944972912008962 0.0 32-33 0.0 9.509563292324926E-5 0.0 0.007940485349091314 0.0 34-35 0.0 9.509563292324926E-5 0.0 0.010793354336788791 0.0 36-37 0.0 9.509563292324926E-5 0.0 0.01488246655248851 0.0 38-39 0.0 9.509563292324926E-5 0.0 0.022109734654655454 0.0 40-41 0.0 9.509563292324926E-5 0.0 0.032047228295135004 0.0 42-43 0.0 9.509563292324926E-5 0.0 0.04150924377099831 0.0 44-45 0.0 9.509563292324926E-5 0.0 0.04683459921470026 0.0 46-47 0.0 9.509563292324926E-5 0.0 0.05330110225348121 0.0 48-49 0.0 9.509563292324926E-5 0.0 0.06338123934334564 0.0 50-51 0.0 9.509563292324926E-5 0.0 0.07346137643321006 0.0 52-53 0.0 9.509563292324926E-5 0.0 0.08663212159308009 0.0 54-55 0.0 1.426434493848739E-4 0.0 0.09628432833478988 0.0 56-57 0.0 1.9019126584649852E-4 0.0 0.12424244441422516 0.0 58-59 0.0 1.9019126584649852E-4 0.0 0.1750710602117019 0.0 60-61 0.0 1.9019126584649852E-4 0.0 0.21277647866577024 0.0 62-63 0.0 1.9019126584649852E-4 0.0 0.2336024222759618 0.0 64-65 0.0 1.9019126584649852E-4 0.0 0.27649055272434725 0.0 66-67 0.0 1.9019126584649852E-4 0.0 0.3696367251726699 0.0 68-69 0.0 1.9019126584649852E-4 0.0 0.4941169086692032 0.0 70-71 0.0 1.9019126584649852E-4 0.0 0.5989598439670856 0.0 72-73 0.0 1.9019126584649852E-4 0.0 0.7245336272422362 0.0 74-75 0.0 2.3773908230812315E-4 0.0 0.8743567969128154 0.0 76-77 0.0 2.852868987697478E-4 0.0 0.9691671429372949 0.0 78-79 0.0 2.852868987697478E-4 0.0 1.0703488963676322 0.0 80-81 0.0 2.852868987697478E-4 0.0 1.1978721401177093 0.0 82-83 0.0 2.852868987697478E-4 0.0 1.324824810070247 0.0 84-85 0.0 2.852868987697478E-4 0.0 1.4959494015156343 0.0 86-87 0.0 2.852868987697478E-4 0.0 1.7925526806032486 0.0 88-89 0.0 2.852868987697478E-4 0.0 2.1748371249547107 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1230 0.0 40.159225 2 GTACCTG 1365 0.0 36.89738 1 ACCTGGG 1820 0.0 25.312601 3 GTACATG 4445 0.0 24.15789 1 GGGCGCG 160 1.862205E-6 23.745745 7 TACATGG 4625 0.0 22.900776 2 GAGTACT 4810 0.0 22.074598 12-13 ACATGGG 4620 0.0 21.690866 3 TACGAGA 110 0.0028801989 21.58704 4 TCGCGCT 125 9.484211E-9 20.903212 60-61 CGATCGT 125 9.509677E-9 20.898243 24-25 TCTATAC 370 0.0 20.537838 3 CCCGCGA 130 1.4941179E-8 20.09351 20-21 GTACTTT 5255 0.0 20.069687 14-15 GTACACG 215 1.0755521E-6 19.889614 1 CTCGCGC 135 2.2973836E-8 19.354826 60-61 AGTACTT 5060 0.0 19.340916 12-13 GTATCAA 9895 0.0 19.015236 1 GTACCGG 150 7.705081E-4 19.005632 1 CGCGCTT 125 2.175002E-7 18.999306 62-63 >>END_MODULE