FastQCFastQC Report
Thu 26 May 2016
SRR936528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104374
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.3127563669553974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.24919094437210582No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22000.19920787704165435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.15049249620146798No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16100.14578394638048342No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12790.11581221578921633No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11490.10404084123675494No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11200.10141491922120585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG14400.041.9246031
TACCTGG14350.040.4143562
GTACACG1754.9203663E-927.1637481
ACCTGGG20600.026.53863
GTACATG51100.026.0474281
TACATGG53950.023.8784182
ACATGGG52900.023.2750993
TGTACCG1302.9243494E-421.9419385
GTGTAGC6950.021.8873351
CGTATAG1100.002864477521.6075271
GAGTACT35700.021.56925612-13
GTATCAA84750.020.080341
CATGGGA32800.020.0001374
GTACTTT39400.019.23906314-15
GTACCGG1250.006004253419.0146241
TACACGG2302.0426214E-618.6012612
AGTACTT38500.018.45732912-13
CATGGGG21000.018.335534
TCAACGC93650.018.0704924
CAACGCA93700.017.9102755
Produced by FastQC (version 0.11.5)