##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936528_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1104374 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29693835602794 31.0 31.0 34.0 28.0 34.0 2 31.35165170494778 31.0 31.0 34.0 28.0 34.0 3 31.31478647632052 31.0 31.0 34.0 28.0 34.0 4 34.98405612591387 37.0 35.0 37.0 32.0 37.0 5 35.01906872128464 37.0 35.0 37.0 32.0 37.0 6 35.07372502431242 37.0 35.0 37.0 32.0 37.0 7 34.9435571645113 37.0 35.0 37.0 32.0 37.0 8 34.92917345029854 37.0 35.0 37.0 32.0 37.0 9 36.412929858906494 39.0 37.0 39.0 32.0 39.0 10-11 36.28189182287885 39.0 36.0 39.0 32.0 39.0 12-13 36.18754244486017 39.0 35.0 39.0 32.0 39.0 14-15 37.14806714029849 39.0 36.5 41.0 31.0 41.0 16-17 37.05969218761036 39.0 36.0 41.0 31.0 41.0 18-19 36.941679177525 39.0 36.0 41.0 31.0 41.0 20-21 36.92548991555397 39.0 36.0 41.0 31.0 41.0 22-23 36.90846352775418 39.0 36.0 41.0 30.5 41.0 24-25 37.02266261248454 39.0 36.0 41.0 31.0 41.0 26-27 36.94388449927289 39.0 36.0 41.0 30.5 41.0 28-29 36.82017369115897 39.0 36.0 41.0 30.0 41.0 30-31 36.62819026887631 39.0 36.0 41.0 30.0 41.0 32-33 36.46353273438165 39.0 35.0 41.0 30.0 41.0 34-35 36.2625414940953 39.0 35.0 40.0 30.0 41.0 36-37 36.05338227810506 38.5 35.0 40.0 29.5 41.0 38-39 35.8493626253425 38.0 35.0 40.0 28.5 41.0 40-41 35.63165060024955 38.0 35.0 40.0 27.5 41.0 42-43 35.36245873227729 38.0 34.5 40.0 27.0 41.0 44-45 35.221802577749926 38.0 34.0 40.0 26.0 41.0 46-47 35.06924646904038 38.0 34.0 40.0 25.5 41.0 48-49 34.847595560924105 38.0 34.0 40.0 24.0 41.0 50-51 33.90183941309738 37.5 33.0 39.5 23.5 40.5 52-53 34.029491820705665 37.5 33.0 39.5 23.5 40.5 54-55 34.48258379860446 38.0 34.0 40.0 24.0 41.0 56-57 34.339174953412524 38.0 34.0 40.0 23.0 41.0 58-59 34.1279303931458 38.0 33.5 40.0 23.0 41.0 60-61 33.84360687593153 38.0 33.0 40.0 22.0 41.0 62-63 33.5139957115977 37.0 33.0 40.0 21.5 41.0 64-65 33.157239304800726 36.5 32.0 39.5 20.5 41.0 66-67 32.79040479040615 36.0 32.0 39.0 19.5 41.0 68-69 32.35199352755498 35.5 31.0 39.0 18.0 40.5 70-71 31.947123891000693 35.0 31.0 38.5 16.0 40.0 72-73 31.515808956023953 35.0 31.0 37.5 12.5 40.0 74-75 31.082802565073067 35.0 30.5 37.0 9.5 39.0 76-77 30.599066982743164 35.0 30.0 36.5 7.0 39.0 78-79 30.12054385561413 34.0 29.5 36.0 6.0 38.5 80-81 29.652261371600563 34.0 29.0 36.0 2.0 37.5 82-83 29.223543382948165 34.0 29.0 35.0 2.0 37.0 84-85 28.8406477334671 34.0 29.0 35.0 2.0 36.5 86-87 28.48208079871493 34.0 29.0 35.0 2.0 36.0 88-89 28.101609599646498 34.0 27.5 35.0 2.0 36.0 90-91 27.719383560279397 34.0 27.0 35.0 2.0 35.5 92-93 27.284249719750736 33.0 26.0 35.0 2.0 35.0 94-95 26.780755432489357 33.0 25.0 35.0 2.0 35.0 96-97 26.17308719690974 33.0 24.5 35.0 2.0 35.0 98-99 25.41390643024917 32.5 21.0 35.0 2.0 35.0 100-101 22.789236707854403 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5871955573949386 1101 2 0.49253878650078775 1101 3 0.6797330379297684 1101 4 0.664495756787808 1101 5 0.5496865248083438 1101 6 0.6893353730617946 1101 7 0.6354189177267102 1101 8 0.6176461577897641 1101 9 0.9042378259722597 1101 10-11 0.9067187378122128 1101 12-13 0.9020372299720165 1101 14-15 0.981869789724449 1101 16-17 1.1535597440117087 1101 18-19 1.4004007980949709 1101 20-21 1.187273268183418 1101 22-23 1.1312455851432688 1101 24-25 0.984378696471417 1101 26-27 0.8105387189833522 1101 28-29 0.9822691811746722 1101 30-31 0.9227223998170118 1101 32-33 0.9852525030303241 1101 34-35 1.2665805883343353 1101 36-37 1.2615456928701576 1101 38-39 1.1450056938508126 1101 40-41 1.1876888147169353 1101 42-43 1.185931918706764 1101 44-45 1.1222879755212176 1101 46-47 1.4050485708669598 1101 48-49 1.3038410051060865 1101 50-51 1.4495214196184492 1101 52-53 1.2647519367241387 1101 54-55 1.404968485939584 1101 56-57 1.4460304244107078 1101 58-59 1.3911795685684964 1101 60-61 1.2847513045721826 1101 62-63 1.5060020919561339 1101 64-65 1.4373594400723562 1101 66-67 1.5671157621726834 1101 68-69 1.5733569639088998 1101 70-71 1.4139667122871344 1101 72-73 1.365633114759504 1101 74-75 1.1836715658181305 1101 76-77 1.3049111588320912 1101 78-79 1.3080569535801132 1101 80-81 1.2950024589362137 1101 82-83 1.419457304921135 1101 84-85 1.4519589812205531 1101 86-87 1.374060472564551 1101 88-89 1.577167880050311 1101 90-91 1.2779549092928946 1101 92-93 1.4489564345374681 1101 94-95 1.6143891426078056 1101 96-97 1.6714607726602324 1101 98-99 1.7908901631579184 1101 100-101 1.9125819287536068 1102 1 0.43781196499714525 1102 2 0.4722235122240903 1102 3 0.5559279855672585 1102 4 0.3099789432154765 1102 5 0.4900616419509376 1102 6 0.5037964053326007 1102 7 0.5312975477498654 1102 8 0.39864586155639614 1102 9 0.8253857209454267 1102 10-11 0.5466629192486252 1102 12-13 0.48518471878873726 1102 14-15 0.674033951449232 1102 16-17 0.6062946859481002 1102 18-19 0.7329786431928724 1102 20-21 0.6849583628010976 1102 22-23 0.5475792221623763 1102 24-25 0.46251704676829775 1102 26-27 0.5663175984759903 1102 28-29 0.37219240270319887 1102 30-31 0.3572306058538359 1102 32-33 0.35954522258303 1102 34-35 0.23482958831383627 1102 36-37 0.35701853907659853 1102 38-39 0.04921425704447557 1102 40-41 0.27512889266917284 1102 42-43 0.27664453694392677 1102 44-45 0.6482866452657348 1102 46-47 0.5524460955484116 1102 48-49 0.5744380736999446 1102 50-51 0.6910289307178843 1102 52-53 0.39621061589595286 1102 54-55 0.7531805592191532 1102 56-57 0.5502784853863432 1102 58-59 0.675503047950464 1102 60-61 0.9280565236824003 1102 62-63 0.8642525637869483 1102 64-65 0.8506040524444813 1102 66-67 0.6318185320477951 1102 68-69 0.80960027769828 1102 70-71 0.5138830589658738 1102 72-73 0.7328590849237742 1102 74-75 0.6874182657970778 1102 76-77 0.7414352422320292 1102 78-79 0.5822805520143604 1102 80-81 0.6925299350465792 1102 82-83 0.5421873468968421 1102 84-85 0.6797121349880264 1102 86-87 0.5462968854207055 1102 88-89 0.6562547360454971 1102 90-91 0.7427478533495027 1102 92-93 1.0015890921233712 1102 94-95 0.6850687466611731 1102 96-97 0.8061938552199166 1102 98-99 1.066598654622112 1102 100-101 1.523517597672761 1103 1 0.33137959625734226 1103 2 0.4205194871918394 1103 3 0.34515632839239885 1103 4 0.4155783154090358 1103 5 0.3845093317091397 1103 6 0.3881145982477747 1103 7 0.5772500140020327 1103 8 0.47541567037310273 1103 9 0.4673919248872167 1103 10-11 0.40627270507557256 1103 12-13 0.4924302004001646 1103 14-15 0.7324148908881511 1103 16-17 0.8125569257322738 1103 18-19 1.0544844256360548 1103 20-21 0.9729245629073446 1103 22-23 0.7697672264238307 1103 24-25 0.6632177404026365 1103 26-27 0.6347880320989319 1103 28-29 0.7064093241809175 1103 30-31 0.6482734810567194 1103 32-33 0.7095626060914597 1103 34-35 0.8429711883143156 1103 36-37 0.7369617353008238 1103 38-39 0.5709156995521241 1103 40-41 0.6424662509759287 1103 42-43 0.7556443175175716 1103 44-45 0.7171856367869367 1103 46-47 0.8181714055249003 1103 48-49 0.8498231434133956 1103 50-51 0.8972411671783078 1103 52-53 0.6819178423174677 1103 54-55 0.6165414767389592 1103 56-57 0.7444455094297098 1103 58-59 0.7736301587525389 1103 60-61 0.5351651138416287 1103 62-63 0.582563251497362 1103 64-65 0.5681885154117197 1103 66-67 0.7191158053371396 1103 68-69 0.8752264046114178 1103 70-71 0.7776158835595055 1103 72-73 0.7950526307300549 1103 74-75 0.7128717224091901 1103 76-77 0.7187554994020644 1103 78-79 0.6411588774559931 1103 80-81 0.8521893712326296 1103 82-83 0.6562446342341772 1103 84-85 0.8680172988018562 1103 86-87 0.6410925983122091 1103 88-89 0.5990756411997822 1103 90-91 0.8322435080606674 1103 92-93 0.9056121242425661 1103 94-95 0.8568169275696675 1103 96-97 1.2055136335345935 1103 98-99 1.17797194002285 1103 100-101 1.3687676054674007 1104 1 0.20539917102809468 1104 2 0.19829701085411955 1104 3 0.2996985602913398 1104 4 0.35995294162102454 1104 5 0.11044310133336666 1104 6 0.13862089160861046 1104 7 0.09223012247631601 1104 8 0.2809332674264766 1104 9 0.43444197560231856 1104 10-11 0.3413696698311455 1104 12-13 0.31332099083530096 1104 14-15 0.532258628916928 1104 16-17 0.2712146851425885 1104 18-19 0.3785073166140265 1104 20-21 0.5642526238523686 1104 22-23 0.12148416412373564 1104 24-25 0.3345844637497706 1104 26-27 0.04667040279829848 1104 28-29 -0.1470937913385839 1104 30-31 0.02307665272304149 1104 32-33 0.047502989833809295 1104 34-35 0.17050525662589422 1104 36-37 0.25700420562362325 1104 38-39 0.17294453225861872 1104 40-41 0.2385599303993189 1104 42-43 0.3752773506007472 1104 44-45 0.4153476003475305 1104 46-47 0.5440151229703147 1104 48-49 0.5470354138375129 1104 50-51 0.6467311573783405 1104 52-53 0.7065612642773971 1104 54-55 0.7743614935944478 1104 56-57 0.7272661266330473 1104 58-59 0.6346299037476442 1104 60-61 0.671432928449633 1104 62-63 0.565089412370007 1104 64-65 0.5987102005481404 1104 66-67 0.7387280263612084 1104 68-69 0.6740552688790657 1104 70-71 0.551440259512205 1104 72-73 0.9407439055601543 1104 74-75 0.8751160171033732 1104 76-77 0.8606119808147241 1104 78-79 0.6131469322488883 1104 80-81 0.6940823309247044 1104 82-83 0.9168096130276062 1104 84-85 0.9839790617890962 1104 86-87 0.8030161357218866 1104 88-89 0.5860489503057451 1104 90-91 0.6685683843032457 1104 92-93 0.6746382914045661 1104 94-95 0.7401944960579243 1104 96-97 0.5588114872624104 1104 98-99 0.4013996861568927 1104 100-101 1.2707042133075994 1105 1 0.2353345439902803 1105 2 0.14017761271861673 1105 3 0.2317560598045354 1105 4 0.25611299637368745 1105 5 0.24410665590109915 1105 6 0.20779509656009054 1105 7 0.37235992960913933 1105 8 0.3478295680499386 1105 9 0.19047634810707592 1105 10-11 0.3159224136285488 1105 12-13 0.24488448352976633 1105 14-15 0.558867305232134 1105 16-17 0.703775121043833 1105 18-19 0.4196343204381847 1105 20-21 0.28168216378943356 1105 22-23 0.3621176220101603 1105 24-25 0.24101352321839187 1105 26-27 0.14909745973898936 1105 28-29 0.3666496799940404 1105 30-31 0.06415180743503157 1105 32-33 0.11617310619474353 1105 34-35 0.03333641402481646 1105 36-37 -0.0936161978085508 1105 38-39 -0.11791919329596823 1105 40-41 -0.09000530096870563 1105 42-43 -0.2810330320111305 1105 44-45 0.11470311588325899 1105 46-47 0.023150752182338863 1105 48-49 0.27771124973162387 1105 50-51 0.19529044338150925 1105 52-53 0.3346049511871101 1105 54-55 0.03833915008370781 1105 56-57 -0.018392950475799807 1105 58-59 0.1059069358583713 1105 60-61 0.04191528650116538 1105 62-63 0.14062406313166775 1105 64-65 0.38623253887396913 1105 66-67 0.18363863273081193 1105 68-69 -0.01391014891532194 1105 70-71 0.13225228574094672 1105 72-73 -0.13300684769222215 1105 74-75 -0.00995120815105821 1105 76-77 0.12967833961288733 1105 78-79 0.10908282600659902 1105 80-81 0.25880788205196126 1105 82-83 0.11994550494179279 1105 84-85 -0.05686450022186662 1105 86-87 0.14899373920983194 1105 88-89 0.17266951735067693 1105 90-91 0.3172483059073876 1105 92-93 0.5499861125320429 1105 94-95 0.5438511258886791 1105 96-97 0.5930497604334874 1105 98-99 0.7352382602832677 1105 100-101 1.0731904934564191 1106 1 -0.09390562482185416 1106 2 -0.09876572220395374 1106 3 0.07377015102665752 1106 4 0.17840114244209815 1106 5 0.17011537903860585 1106 6 -0.01419462638317981 1106 7 0.21476250063913938 1106 8 0.31338906726414706 1106 9 0.33252222404075127 1106 10-11 0.36432081732970545 1106 12-13 0.3391623525414431 1106 14-15 0.09483347217250326 1106 16-17 0.14919812258009557 1106 18-19 0.14370064453629539 1106 20-21 -0.1528545740396723 1106 22-23 -0.14084188779894902 1106 24-25 -0.4902387944168183 1106 26-27 -0.555965138484595 1106 28-29 -0.4549974743913694 1106 30-31 -0.4267780719596459 1106 32-33 -0.22786196293700556 1106 34-35 -0.3444081075269807 1106 36-37 -0.4166221486128805 1106 38-39 -0.39751340847846706 1106 40-41 -0.3538210555907284 1106 42-43 -0.658134796062626 1106 44-45 -0.6649361276406083 1106 46-47 -0.45939149499676546 1106 48-49 -0.6700947222625118 1106 50-51 -0.5911198266119584 1106 52-53 -0.590152690387292 1106 54-55 -0.3380880717559265 1106 56-57 -0.11027195667382017 1106 58-59 -0.39109735507769017 1106 60-61 -0.11972409828807429 1106 62-63 -0.1838130826012332 1106 64-65 -0.3767365063486139 1106 66-67 -0.46086614380501345 1106 68-69 -0.17238872072228872 1106 70-71 -0.2655951829599452 1106 72-73 -0.38339003004817584 1106 74-75 -0.17506925303813858 1106 76-77 -0.2621856571380121 1106 78-79 -0.07589801487560521 1106 80-81 -0.04791811607627139 1106 82-83 0.12870042715753982 1106 84-85 0.35014402867573224 1106 86-87 0.43190769093238757 1106 88-89 0.49862261724296175 1106 90-91 0.5578868254461575 1106 92-93 0.6589997269834917 1106 94-95 0.9193742492663333 1106 96-97 1.1313468062211314 1106 98-99 0.8150673291677997 1106 100-101 1.1168158929725855 1107 1 0.2646583781278373 1107 2 0.32545773505247055 1107 3 0.25715654286823764 1107 4 0.43038589036921593 1107 5 0.35551079981955525 1107 6 0.10502514375657057 1107 7 0.30357884625686893 1107 8 0.39273581810498825 1107 9 0.5221695198554244 1107 10-11 0.6458159881061576 1107 12-13 0.4376272529892944 1107 14-15 -0.006727706985394377 1107 16-17 0.19662253668825969 1107 18-19 0.28926261507533724 1107 20-21 0.10204659324751475 1107 22-23 0.12446684077529824 1107 24-25 0.13827880482057253 1107 26-27 0.015665646241750153 1107 28-29 0.20093698486149236 1107 30-31 0.16221003933731737 1107 32-33 0.1774052181274115 1107 34-35 0.08095835589862332 1107 36-37 0.14897885140509715 1107 38-39 0.14998384866644443 1107 40-41 0.18712122754385518 1107 42-43 0.0867859758937044 1107 44-45 0.04974848898918793 1107 46-47 0.15618882119597544 1107 48-49 0.3718628669027595 1107 50-51 0.19647167683234557 1107 52-53 0.037449424758655425 1107 54-55 0.006739173338409898 1107 56-57 0.33683680427512286 1107 58-59 0.37074458452553927 1107 60-61 0.44067626862499765 1107 62-63 0.29515776219813006 1107 64-65 0.3335694344057174 1107 66-67 0.3003534824472638 1107 68-69 0.23649812976064055 1107 70-71 0.530354384478315 1107 72-73 0.3473614223160766 1107 74-75 0.23100279861746742 1107 76-77 0.6289877452613126 1107 78-79 0.5641827419725409 1107 80-81 0.5278710168218019 1107 82-83 0.5020758475787694 1107 84-85 0.6450198357945673 1107 86-87 0.6853151263179171 1107 88-89 0.6436531034027837 1107 90-91 0.5790760554682812 1107 92-93 0.9014959949019818 1107 94-95 0.777622599056798 1107 96-97 0.9407102839815238 1107 98-99 1.0163533235202138 1107 100-101 1.5946491273832741 1108 1 -0.03566349075892816 1108 2 -0.0664621806322856 1108 3 0.1353638582769996 1108 4 0.15964449249529622 1108 5 -0.03475238201930608 1108 6 0.13423218620232547 1108 7 0.2266336056703011 1108 8 0.3451720130005782 1108 9 -0.2758985085933858 1108 10-11 0.029834193222697536 1108 12-13 -0.1317792799255031 1108 14-15 0.3579129675365422 1108 16-17 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2315 82-83 -2.086164732794419 2315 84-85 -1.9680075162527508 2315 86-87 -2.3940885968719563 2315 88-89 -2.195115782145617 2315 90-91 -2.1663625467560443 2315 92-93 -1.9641933071905449 2315 94-95 -2.3680752539545438 2315 96-97 -2.013041227406994 2315 98-99 -2.37667849461209 2315 100-101 -2.6970366018412584 2316 1 -5.3030281673028625 2316 2 -5.238505519996295 2316 3 -6.176387400830134 2316 4 -7.569871586588043 2316 5 -8.764857266866013 2316 6 -9.512435458411634 2316 7 -11.474636095892784 2316 8 -11.621993406783293 2316 9 -11.744123371273286 2316 10-11 -11.544843287911071 2316 12-13 -9.831983426422006 2316 14-15 -9.445283290185166 2316 16-17 -8.408385631213715 2316 18-19 -8.846230535266134 2316 20-21 -9.948182146581182 2316 22-23 -9.555872141621762 2316 24-25 -9.609544213040806 2316 26-27 -7.748766081862584 2316 28-29 -5.9723078805465235 2316 30-31 -8.37589462167064 2316 32-33 -6.061863200154036 2316 34-35 -4.479251232273256 2316 36-37 -5.1249269679598655 2316 38-39 -4.090576293900568 2316 40-41 -3.9238233753726703 2316 42-43 -4.132402614887656 2316 44-45 -4.327886310323436 2316 46-47 -4.372870394646867 2316 48-49 -4.599050433758968 2316 50-51 -4.621933296812429 2316 52-53 -4.590939483095447 2316 54-55 -4.16102008281355 2316 56-57 -4.728507375877747 2316 58-59 -5.025375999551777 2316 60-61 -5.4545807550956695 2316 62-63 -4.929631675653482 2316 64-65 -5.970329075313799 2316 66-67 -5.177368492584222 2316 68-69 -5.302920917362467 2316 70-71 -4.896078084233892 2316 72-73 -4.176396854073381 2316 74-75 -4.497582736770667 2316 76-77 -4.995418466587253 2316 78-79 -5.150385246409172 2316 80-81 -5.7576979301070885 2316 82-83 -5.2602922075348 2316 84-85 -5.306097151320014 2316 86-87 -5.654350500797587 2316 88-89 -7.161567024930672 2316 90-91 -6.1061053259582785 2316 92-93 -5.5343772955763875 2316 94-95 -4.938055818973918 2316 96-97 -6.330999266581358 2316 98-99 -7.764002438274062 2316 100-101 -8.646120757648923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3019.0 3 1149.0 4 1135.0 5 1245.0 6 1314.0 7 1628.0 8 1961.0 9 2524.0 10 3281.0 11 3920.0 12 4966.0 13 5990.0 14 6510.0 15 7538.0 16 9159.0 17 11042.0 18 9045.0 19 7357.0 20 7551.0 21 8113.0 22 9108.0 23 10217.0 24 11517.0 25 13276.0 26 15751.0 27 18379.0 28 21592.0 29 26062.0 30 31403.0 31 38616.0 32 47618.0 33 61006.0 34 79058.0 35 107062.0 36 147760.0 37 192912.0 38 153014.0 39 21569.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.010620328686485 18.718963629905105 13.963285559977672 22.307130481430736 2 15.129038656798746 20.25815883171852 40.62473676440085 23.988065747081876 3 19.47883998699545 25.537818695216302 29.64801685157698 25.33532446621127 4 12.370316095883897 16.465768883470833 38.27947328522897 32.88444173541631 5 13.288680569707672 37.55378937119308 34.24088037497452 14.91664968412472 6 30.651304556550407 36.11354918184988 18.78987151442689 14.445274747172821 7 28.49725062617578 32.59684429156049 21.11553532138857 17.790369760875162 8 26.326904469401292 33.79058356254734 20.48756446077632 19.394947507275052 9 26.615161985123088 16.956403153710305 20.98552993913495 35.44290492203166 10-11 24.494585933302467 26.573911908953434 27.935469724988426 20.99603243275567 12-13 25.99322143215929 24.030353635067016 28.178886070147264 21.797538862626432 14-15 22.751811880573733 25.430283419294142 26.884724944276833 24.933179755855292 16-17 21.685430567075528 28.716083530877878 27.457702515810734 22.14078338623586 18-19 21.433169129720852 27.38313798765642 29.795776003623804 21.387916878998926 20-21 22.39588144622134 26.482692039479282 29.559381039582526 21.562045474716847 22-23 21.913474312799814 26.472319550237405 29.58123646677721 22.03296967018557 24-25 21.95989211337153 26.573105145989302 29.74059931782901 21.72640342281016 26-27 21.7788338310188 26.62363657277212 29.80529131581879 21.792238280390286 28-29 21.42953518486657 27.024024051217506 29.864349684418322 21.682091079497603 30-31 21.601266249744764 26.68876687793467 29.743558167215777 21.966408705104794 32-33 21.356389602039382 26.77067344046439 29.728006665127165 22.144930292369065 34-35 22.12703933942405 26.742802910757856 29.395511970358122 21.734645779459967 36-37 22.126166034534045 26.365250257961726 29.495246214788057 22.013337492716175 38-39 21.867281484074077 26.763166557168915 29.56973497318852 21.799816985568484 40-41 21.956915548444087 26.672896747725634 29.652152298315844 21.718035405514435 42-43 21.639268110144776 26.922981147674214 29.14796216586008 22.28978857632093 44-45 21.8714544297161 26.883900428388646 29.027971578391682 22.21667356350357 46-47 22.112430871064205 27.1242136335957 28.545977675004018 22.217377820336072 48-49 22.113114776364696 27.229131672952327 28.39491472475133 22.262838825931645 50-51 21.96473902888129 27.3838235620363 28.483957530364723 22.16747987871769 52-53 22.182933913089933 27.551190880967134 28.190785437690124 22.075089768252802 54-55 21.7712320797557 27.284815585321077 28.408032615806718 22.535919719116514 56-57 22.08827977144513 27.679226318220095 27.891526989215183 22.34096692111959 58-59 22.336428710490928 27.220091729411816 28.07943241860486 22.364047141492392 60-61 22.109502518535287 27.233323900616902 28.64714471673552 22.01002886411229 62-63 22.042054849781795 27.29087462370177 28.56366701121526 22.103403515301174 64-65 21.823971212559552 27.489144268160004 28.369917523150068 22.316966996130375 66-67 21.778338379960303 27.589535372267214 28.047217956333398 22.584908291439085 68-69 21.86734834325785 27.448258501035916 28.32933092826821 22.355062227438026 70-71 21.94062395017526 27.39536614717603 28.110265667346706 22.553744235302002 72-73 21.924001383834852 27.268746524563976 28.194457777640924 22.61279431396025 74-75 21.752071055485494 27.747592730532983 28.010496215611646 22.48983999836987 76-77 21.875370769379863 27.46795420437759 28.07997840820271 22.576696618039836 78-79 21.888181331193604 27.491793754604622 28.08833990904641 22.531685005155367 80-81 21.89073581289333 27.79197269504654 27.840285220487587 22.477006271572538 82-83 21.977310986006813 27.65033418664648 28.016078629169183 22.356276198177525 84-85 21.790188045038807 27.670642055206905 27.86908700382537 22.670082895928914 86-87 21.90953649951963 27.60524902726202 27.97365344720955 22.5115610260088 88-89 21.973972066877227 27.399235026513285 27.70900944626351 22.917783460345976 90-91 21.828897068112184 27.362143135602697 28.079754520671003 22.729205275614117 92-93 21.97925001007479 27.36127015693578 28.14121729712205 22.518262535867382 94-95 21.628455405302603 27.2312744291857 28.153923340807747 22.98634682470395 96-97 21.521778111042284 27.159834744273827 28.258566165683174 23.059820979000715 98-99 21.494167039892183 27.41733526489804 27.871772240806763 23.21672545440301 100-101 21.39929827230802 27.27844134578112 28.101214347559893 23.221046034350966 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 90.0 1 85.5 2 179.5 3 276.0 4 222.5 5 183.0 6 276.5 7 488.0 8 785.0 9 954.0 10 863.5 11 799.0 12 873.0 13 962.0 14 1018.5 15 1086.5 16 1153.5 17 1260.0 18 1408.5 19 1494.5 20 1657.0 21 1965.5 22 2307.5 23 2719.5 24 3320.0 25 4182.0 26 5354.0 27 6872.5 28 8492.5 29 10330.5 30 12735.5 31 15572.5 32 18873.0 33 21629.0 34 24250.0 35 27128.0 36 30280.0 37 33400.0 38 34570.0 39 34931.0 40 35495.0 41 36452.0 42 37598.5 43 38984.0 44 40322.0 45 41597.5 46 42406.0 47 43837.5 48 49611.5 49 49103.5 50 42175.0 51 38226.0 52 35377.5 53 33777.0 54 32276.5 55 29797.0 56 26881.5 57 23765.5 58 21366.5 59 18824.0 60 15802.5 61 13051.0 62 10641.0 63 8196.5 64 5753.0 65 4433.5 66 3394.0 67 2510.5 68 1924.5 69 1383.0 70 1013.0 71 794.0 72 639.0 73 542.0 74 443.5 75 330.0 76 237.0 77 147.5 78 83.0 79 53.0 80 36.0 81 31.5 82 25.0 83 16.0 84 8.0 85 4.5 86 3.5 87 4.5 88 4.0 89 3.0 90 3.5 91 2.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07515569906571506 2 0.029428436381153486 3 0.013129610077745402 4 0.02236561164967665 5 0.027074161470661207 6 0.03250710357179723 7 0.07696667976609373 8 0.05451051908139815 9 0.07108099248986303 10-11 0.06112059863778032 12-13 0.04106398738108648 14-15 0.04341826229157876 16-17 0.05564238201913482 18-19 0.050843283163131324 20-21 0.01810980700378676 22-23 0.01331070814778327 24-25 0.02281835682477132 26-27 0.02381439620997959 28-29 0.005795138241211763 30-31 9.507648676988049E-4 32-33 0.010775335167253122 34-35 0.011363903894876192 36-37 0.003938883023323621 38-39 0.008104138634194576 40-41 0.004934922408531892 42-43 0.0024900984630206796 44-45 2.716471050568014E-4 46-47 5.885687276230697E-4 48-49 8.149413151704042E-4 50-51 7.696667976609373E-4 52-53 2.2637258754733454E-4 54-55 1.358235525284007E-4 56-57 0.003984157540833087 58-59 0.0035314123657384185 60-61 0.005568765653664429 62-63 0.0027617455680774812 64-65 3.169216225662683E-4 66-67 5.432942101136028E-4 68-69 0.0034408633307194845 70-71 0.0020826278054354775 72-73 0.017747610863711023 74-75 0.015076414330652477 76-77 0.022727807789752384 78-79 0.016706296960993287 80-81 0.009552923194497517 82-83 0.004618000785965624 84-85 0.0033955888132100173 86-87 0.0015393335953218746 88-89 0.0019920787704165435 90-91 0.0077419424941188395 92-93 0.01199774714000873 94-95 0.014668943673067276 96-97 0.013220159112764336 98-99 0.023588023622432255 100-101 0.02191286647458198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1104374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.62878478491672 #Duplication Level Percentage of deduplicated Percentage of total 1 85.51290564353067 57.83133892099226 2 9.099485435031289 12.307742842784307 3 2.32491957430996 4.716944565997454 4 0.9478402362181102 2.564051333827168 5 0.5077809473402763 1.7170304202778344 6 0.2980310253358825 1.2093285643001077 7 0.21990938720399972 1.041054323358155 8 0.1504231328049439 0.8138346940110799 9 0.11430388711147377 0.6957209683387149 >10 0.7368845880915079 9.309284350399283 >50 0.058004985745323374 2.66708762279294 >100 0.02749903515405236 3.2926212646781763 >500 9.389903238035516E-4 0.45268406410416 >1k 0.0010731317986326305 1.3812760641382644 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3454 0.3127563669553974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 0.24919094437210582 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2200 0.19920787704165435 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1662 0.15049249620146798 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1610 0.14578394638048342 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1279 0.11581221578921633 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1149 0.10404084123675494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1120 0.10141491922120585 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 9.05490350189338E-5 0.0 0.0 0.0 3 0.0 9.05490350189338E-5 0.0 0.0 0.0 4 0.0 9.05490350189338E-5 0.0 0.0 0.0 5 0.0 9.05490350189338E-5 0.0 0.0 0.0 6 0.0 9.05490350189338E-5 0.0 0.0 0.0 7 0.0 9.05490350189338E-5 0.0 0.0 0.0 8 0.0 9.05490350189338E-5 0.0 0.0 0.0 9 0.0 9.05490350189338E-5 0.0 0.0 0.0 10-11 0.0 9.05490350189338E-5 0.0 0.0 0.0 12-13 0.0 9.05490350189338E-5 0.0 0.0 0.0 14-15 0.0 9.05490350189338E-5 0.0 0.0 0.0 16-17 0.0 9.05490350189338E-5 0.0 0.0 0.0 18-19 0.0 9.05490350189338E-5 0.0 0.0 0.0 20-21 0.0 2.716471050568014E-4 0.0 9.05490350189338E-5 0.0 22-23 0.0 2.716471050568014E-4 0.0 2.716471050568014E-4 0.0 24-25 0.0 2.716471050568014E-4 0.0 5.432942101136028E-4 0.0 26-27 0.0 2.716471050568014E-4 0.0 7.696667976609373E-4 0.0 28-29 0.0 2.716471050568014E-4 0.0 0.0017204316653597423 0.0 30-31 0.0 2.716471050568014E-4 0.0 0.0040747065758520214 0.0 32-33 0.0 2.716471050568014E-4 0.0 0.007243922801514705 0.0 34-35 0.0 2.716471050568014E-4 0.0 0.009643472229516451 0.0 36-37 0.0 2.716471050568014E-4 0.0 0.014487845603029407 0.0 38-39 0.0 2.716471050568014E-4 0.0 0.021188474194430512 0.0 40-41 0.0 2.716471050568014E-4 0.0 0.030152828661304955 0.0 42-43 0.0 2.716471050568014E-4 0.0 0.03875498698810367 0.0 44-45 0.0 2.716471050568014E-4 0.0 0.043463536809088224 0.0 46-47 0.0 2.716471050568014E-4 0.0 0.04925867505029999 0.0 48-49 0.0 2.716471050568014E-4 0.0 0.05686479399189043 0.0 50-51 0.0 2.716471050568014E-4 0.0 0.06614607008133114 0.0 52-53 0.0 2.716471050568014E-4 0.0 0.08176577862209722 0.0 54-55 0.0 2.716471050568014E-4 0.0 0.09322023155199236 0.0 56-57 0.0 2.716471050568014E-4 0.0 0.12115460885533344 0.0 58-59 0.0 2.716471050568014E-4 0.0 0.16765153833755592 0.0 60-61 0.0 2.716471050568014E-4 0.0 0.2050482898003756 0.0 62-63 0.0 2.716471050568014E-4 0.0 0.22632731302982503 0.0 64-65 0.0 2.716471050568014E-4 0.0 0.27037941856653636 0.0 66-67 0.0 2.716471050568014E-4 0.0 0.35499749179173 0.0 68-69 0.0 2.716471050568014E-4 0.0 0.47157937437860725 0.0 70-71 0.0 3.169216225662683E-4 0.0 0.5695987047866031 0.0 72-73 0.0 3.621961400757352E-4 0.0 0.6889423329415578 0.0 74-75 0.0 3.621961400757352E-4 0.0 0.8275729055555455 0.0 76-77 0.0 3.621961400757352E-4 0.0 0.9139566849636083 0.0 78-79 0.0 3.621961400757352E-4 0.0 1.011296897608962 0.0 80-81 0.0 3.621961400757352E-4 0.0 1.13122909449154 0.0 82-83 0.0 3.621961400757352E-4 0.0 1.2539230369421954 0.0 84-85 0.0 3.621961400757352E-4 0.0 1.4275055370734915 0.0 86-87 0.0 3.621961400757352E-4 0.0 1.7292601962740883 0.0 88-89 0.0 3.621961400757352E-4 0.0 2.113233379271877 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1440 0.0 41.924603 1 TACCTGG 1435 0.0 40.414356 2 GTACACG 175 4.9203663E-9 27.163748 1 ACCTGGG 2060 0.0 26.5386 3 GTACATG 5110 0.0 26.047428 1 TACATGG 5395 0.0 23.878418 2 ACATGGG 5290 0.0 23.275099 3 TGTACCG 130 2.9243494E-4 21.941938 5 GTGTAGC 695 0.0 21.887335 1 CGTATAG 110 0.0028644775 21.607527 1 GAGTACT 3570 0.0 21.569256 12-13 GTATCAA 8475 0.0 20.08034 1 CATGGGA 3280 0.0 20.000137 4 GTACTTT 3940 0.0 19.239063 14-15 GTACCGG 125 0.0060042534 19.014624 1 TACACGG 230 2.0426214E-6 18.601261 2 AGTACTT 3850 0.0 18.457329 12-13 CATGGGG 2100 0.0 18.33553 4 TCAACGC 9365 0.0 18.070492 4 CAACGCA 9370 0.0 17.910275 5 >>END_MODULE