FastQCFastQC Report
Thu 26 May 2016
SRR936528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104374
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44060.3989590482934223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38700.35042476552327384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29260.2649464764654003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.21604999755517607No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14190.12848908069186707No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13250.1199774714000873No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11710.10603292000717149No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.10250150764143307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12550.040.510952
GTACCTG14250.035.694231
GTACACG1859.094947E-1230.8346231
TATCACG856.395061E-427.9501042
ACCTGGG18500.025.9336683
GTACATG48400.024.0630041
ACATGGG48400.022.4752453
TACATGG51350.022.1151452
GAGTACT49600.021.54450412-13
AGTACTT52100.020.41953812-13
ACGATTC1200.004768310619.788138
GTATCAA105900.019.4815221
GTACTTT54300.019.24149714-15
CATGGGA31250.019.1503124
TACACGG2803.4751793E-818.6666772
GTATAGG4201.8189894E-1218.1092221
CGTACAC1350.00939759317.5934313
AGGACCG1650.001464627517.2712065
TCAACGC119250.017.1263714
CTACACG2504.5637207E-617.0984944
Produced by FastQC (version 0.11.5)