FastQCFastQC Report
Thu 26 May 2016
SRR936527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408682
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.39737497614282014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.3288620492216442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.19036806123098154No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5070.12405733553227202No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4910.12014231113677627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG352.787017E-454.2809561
CGTAACG250.001613809538.008314-15
GTATCAA24050.032.3880161
GGACCGT600.003947323731.6677686
CCTATAC1201.5013211E-731.6677683
GTACACG600.0039496931.6638931
GTATAGA1051.8990049E-631.6638931
GTATTAC650.00584222329.2282091
GGTATCA15500.028.1910761
TCAACGC28100.027.216254
ATCAACG28250.027.0717373
CAACGCA28450.026.8814265
TATCAAC28700.026.6472682
AACGCAG29100.026.444226
GTCCTAA1351.3408833E-524.6274721
TACCTGG3900.024.359822
ACGCAGA32250.024.0085877
GGATATG1201.7077837E-423.7508287
CGCAGAG32750.023.6420448
TAGACTC1852.5324698E-723.1089135
Produced by FastQC (version 0.11.5)