FastQCFastQC Report
Thu 26 May 2016
SRR936527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408682
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.5314645616885499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.47298388478083203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.28457333574759835No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10500.2569234759544095No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.14828154897940207No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4220.10325876843120078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1650.037.4234241
GTAAGGT1553.6379788E-1133.700694
TACCTGG2800.027.1422632
TAGGACC1858.956704E-925.668834
CTAGGAC2251.0186341E-1025.3296783
GTACATG21150.025.1531121
TAAGGTG1901.1896191E-824.9933365
GTATCAA35300.024.8932231
GGTATCA25950.024.7104421
TACATGG21250.024.5876982
ACATGGG20650.024.1490573
GGTAAGG1601.8570026E-623.7465743
GGTAGTA1401.7795115E-523.7436685
GAGTACT21150.023.1318812-13
CTGGTAC1652.4265573E-623.026983
CTAACAC1652.4265573E-623.026983
GTACTTT22150.022.08755314-15
ATGGGAG5900.021.7314935
ATGGGAC2858.367351E-1121.660895
TAGTCGG550.00287702821.59043540-41
Produced by FastQC (version 0.11.5)