##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 408682 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19598612123852 33.0 31.0 34.0 31.0 34.0 2 32.33314166026397 34.0 31.0 34.0 31.0 34.0 3 32.427581836244315 34.0 31.0 34.0 31.0 34.0 4 36.100821665745 37.0 35.0 37.0 35.0 37.0 5 35.980958300096404 37.0 35.0 37.0 35.0 37.0 6 36.049216750431874 37.0 35.0 37.0 35.0 37.0 7 35.99264954169745 37.0 35.0 37.0 35.0 37.0 8 35.96940163745895 37.0 35.0 37.0 35.0 37.0 9 37.635724108230846 39.0 37.0 39.0 35.0 39.0 10-11 37.53738481264161 39.0 37.0 39.0 35.0 39.0 12-13 37.43573242765769 39.0 37.0 39.0 35.0 39.0 14-15 38.596384230281735 40.0 38.0 41.0 34.0 41.0 16-17 38.571608243083865 40.0 38.0 41.0 34.5 41.0 18-19 38.7138447497076 40.0 38.0 41.0 34.5 41.0 20-21 38.72744823603682 40.0 38.0 41.0 34.5 41.0 22-23 38.62809959822062 40.0 38.0 41.0 34.0 41.0 24-25 38.51355699541453 40.0 38.0 41.0 34.0 41.0 26-27 38.31897416573277 40.0 38.0 41.0 34.0 41.0 28-29 38.35263725830842 40.0 38.0 41.0 34.0 41.0 30-31 38.23562941357828 40.0 38.0 41.0 34.0 41.0 32-33 38.035500707151286 40.0 37.0 41.0 33.5 41.0 34-35 37.838095389569396 40.0 37.0 41.0 33.0 41.0 36-37 37.65998869536706 40.0 37.0 41.0 33.0 41.0 38-39 37.46549517717932 40.0 36.5 41.0 33.0 41.0 40-41 37.23022056268688 40.0 36.0 41.0 32.0 41.0 42-43 36.960201330129536 39.0 36.0 41.0 31.5 41.0 44-45 36.643912626443054 39.0 36.0 41.0 31.0 41.0 46-47 36.19477858090153 39.0 35.0 40.5 30.0 41.0 48-49 35.72213603731018 39.0 35.0 40.0 28.5 41.0 50-51 35.42347595440954 39.0 35.0 40.0 27.5 41.0 52-53 35.1863343626585 38.0 35.0 40.0 27.0 41.0 54-55 34.85908848444512 38.0 34.0 40.0 26.0 41.0 56-57 34.52345956024489 38.0 34.0 40.0 25.5 41.0 58-59 34.164601083483 38.0 33.5 40.0 25.0 41.0 60-61 33.79146622557391 37.0 33.0 40.0 23.5 41.0 62-63 33.49238894788613 37.0 33.0 39.0 23.0 41.0 64-65 33.16909602086708 36.0 32.5 39.0 22.0 40.0 66-67 32.73475709720516 36.0 32.0 39.0 21.5 40.0 68-69 32.26642597422935 35.0 31.5 38.5 19.5 40.0 70-71 31.762260143583518 35.0 31.0 38.0 18.0 40.0 72-73 31.209642215708058 35.0 30.5 37.0 15.5 39.0 74-75 30.704935622317596 34.0 30.0 36.5 12.0 39.0 76-77 29.334114299137227 32.5 28.5 35.0 10.0 37.0 78-79 30.081393357182357 34.0 30.0 36.0 8.0 37.5 80-81 29.915982353027537 34.0 30.0 35.0 7.0 37.0 82-83 29.517185733651104 34.0 29.0 35.0 4.0 37.0 84-85 29.08527045477902 34.0 29.0 35.0 2.0 36.0 86-87 28.680990354358645 34.0 29.0 35.0 2.0 36.0 88-89 28.222555678987575 33.0 28.5 35.0 2.0 35.0 90-91 27.695686377183236 33.0 27.0 35.0 2.0 35.0 92-93 27.143303350771504 33.0 26.0 34.0 2.0 35.0 94-95 26.438212350923212 32.0 25.0 34.0 2.0 35.0 96-97 25.6803137892053 32.0 23.5 34.0 2.0 35.0 98-99 24.49294806230761 31.5 10.5 34.0 2.0 35.0 100-101 20.802999642754024 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36566983454458324 1101 2 0.4097629435881416 1101 3 0.36634110621708516 1101 4 0.2442691874256937 1101 5 0.23443242809386788 1101 6 0.3099664232419883 1101 7 0.22598455770541648 1101 8 0.20366163020584338 1101 9 0.29966240130761435 1101 10-11 0.30542950276221603 1101 12-13 0.41063285438367814 1101 14-15 0.2854862744826292 1101 16-17 0.3422526070868699 1101 18-19 0.43360313780502224 1101 20-21 0.26284709860486544 1101 22-23 0.24486552609703693 1101 24-25 0.34272692225192714 1101 26-27 0.15124539579098695 1101 28-29 0.34259600284370606 1101 30-31 0.5514317152729902 1101 32-33 0.3575830837700593 1101 34-35 0.39704741516012376 1101 36-37 0.35475491698334594 1101 38-39 0.26257704588189057 1101 40-41 0.2793672864869947 1101 42-43 0.15503155879704877 1101 44-45 0.48214995319190024 1101 46-47 0.416112563824349 1101 48-49 0.6755202182917657 1101 50-51 0.6715387794223204 1101 52-53 0.6606695271151821 1101 54-55 0.5915754577681298 1101 56-57 0.763577474335726 1101 58-59 0.7408841204048997 1101 60-61 0.6468349387024261 1101 62-63 0.6924464435208222 1101 64-65 0.574116500496892 1101 66-67 0.48227747079111083 1101 68-69 0.513907188857047 1101 70-71 0.28573364275441904 1101 72-73 0.5774588626870631 1101 74-75 0.6101552048822825 1101 76-77 0.542830838281116 1101 78-79 0.711866345885749 1101 80-81 0.8123052394071202 1101 82-83 0.8012006804870744 1101 84-85 0.6103691849635879 1101 86-87 0.6782790952105096 1101 88-89 0.4933594061701285 1101 90-91 0.43447633852475676 1101 92-93 0.5878388894628763 1101 94-95 0.7437276742147283 1101 96-97 1.0659812455264017 1101 98-99 0.6903553484563716 1101 100-101 0.45796426882408525 1102 1 -0.007224678218044289 1102 2 0.1599810997232467 1102 3 0.01755400152193687 1102 4 0.022300435569221122 1102 5 -0.006907004370141578 1102 6 -0.07179427168926367 1102 7 0.24318621846437338 1102 8 0.14400632350010056 1102 9 0.09916734199950383 1102 10-11 0.15476909364520708 1102 12-13 0.09117174294252806 1102 14-15 0.0821878593324854 1102 16-17 0.29155655369967093 1102 18-19 0.22305507414699122 1102 20-21 -0.005679670902125622 1102 22-23 -0.07227540738679039 1102 24-25 -0.051581875942098065 1102 26-27 0.21833451306723362 1102 28-29 0.033674374032571563 1102 30-31 -0.025689283822700304 1102 32-33 0.097099377822083 1102 34-35 -0.09166589269415937 1102 36-37 -0.02403344186203782 1102 38-39 -0.034842705004265895 1102 40-41 -0.24820091618369133 1102 42-43 -0.3042230799086667 1102 44-45 -0.14051848248669074 1102 46-47 -0.10370433023955883 1102 48-49 -0.18102281556368638 1102 50-51 -0.22933516527625386 1102 52-53 -0.27759195661056424 1102 54-55 -0.1402931600922983 1102 56-57 -0.2862782983858523 1102 58-59 -0.17734330324045544 1102 60-61 -0.46673694720950465 1102 62-63 -0.3069707066537646 1102 64-65 -0.08527543465694265 1102 66-67 -0.16993544431612406 1102 68-69 -0.5360799396009455 1102 70-71 -0.6273880692266758 1102 72-73 -0.5667400323405616 1102 74-75 -0.3115521556595091 1102 76-77 -0.45614867946510884 1102 78-79 -0.6222623827359435 1102 80-81 -0.42391263949476965 1102 82-83 -0.22418728099070862 1102 84-85 -0.36790167188506473 1102 86-87 -0.2770474129051621 1102 88-89 -0.4995071192396132 1102 90-91 -0.5794493497727018 1102 92-93 0.05130879315734305 1102 94-95 -0.26743624322257986 1102 96-97 -0.3887848554524993 1102 98-99 -0.6732723141376091 1102 100-101 -0.7413175651396244 1103 1 0.16902700982200258 1103 2 0.14559002949739863 1103 3 0.17453918738403473 1103 4 0.10567184499381455 1103 5 -0.08987226882128851 1103 6 0.033404045516732594 1103 7 0.07340915820938676 1103 8 0.09100609673582483 1103 9 0.02081182299699691 1103 10-11 0.1649227179814119 1103 12-13 -0.014450488079503998 1103 14-15 0.029039558879979666 1103 16-17 -0.17261130601784913 1103 18-19 -0.12027365620997443 1103 20-21 -0.08629521745479707 1103 22-23 0.08486208284033836 1103 24-25 -0.10153770767623627 1103 26-27 0.003677587110850311 1103 28-29 -0.11067039566630399 1103 30-31 0.05689945039264188 1103 32-33 0.07701635502042592 1103 34-35 -0.02439390078998116 1103 36-37 0.0689145275823222 1103 38-39 -0.07766053883004531 1103 40-41 0.2733611512296008 1103 42-43 0.3762169781700635 1103 44-45 0.24903208425477175 1103 46-47 0.09487940839260744 1103 48-49 0.32889304473597747 1103 50-51 0.3389115745642002 1103 52-53 0.4687713536063498 1103 54-55 0.5919143055292295 1103 56-57 0.6098712780387885 1103 58-59 0.3344890536526677 1103 60-61 0.15775241009991703 1103 62-63 0.6541316046495922 1103 64-65 0.46322250584959335 1103 66-67 0.09542445089847007 1103 68-69 0.3645465390206013 1103 70-71 0.1844111191602842 1103 72-73 0.37306166737615243 1103 74-75 0.4746810527842449 1103 76-77 0.1184635366987834 1103 78-79 0.3374082675360093 1103 80-81 0.3292053693118042 1103 82-83 0.25196523998498677 1103 84-85 0.4277490231305201 1103 86-87 0.2505264462505288 1103 88-89 0.3872452778618616 1103 90-91 0.6848511839694851 1103 92-93 0.9097943462421796 1103 94-95 0.7702650100731141 1103 96-97 0.7991984993726717 1103 98-99 0.7239036244803501 1103 100-101 0.5989828467590677 1104 1 0.0797303938833096 1104 2 0.10092744742128446 1104 3 0.038592594503519706 1104 4 -0.025061404890898586 1104 5 -0.06525492468355054 1104 6 -0.06383116980268255 1104 7 -0.08346766722927157 1104 8 0.018386029537722948 1104 9 -0.14666078169808827 1104 10-11 0.046908795237264655 1104 12-13 -0.016524625594051656 1104 14-15 -0.14305456238405156 1104 16-17 0.0038012815482986184 1104 18-19 0.03708080280137693 1104 20-21 0.03127401196303481 1104 22-23 -0.03370743205962867 1104 24-25 0.050718699201688366 1104 26-27 -0.044150552643635876 1104 28-29 0.12213259970010171 1104 30-31 -0.0665133787353156 1104 32-33 -0.15359940798548166 1104 34-35 -0.13523233986150274 1104 36-37 -0.02645626979124671 1104 38-39 -0.21864698940869687 1104 40-41 -0.22084685544628968 1104 42-43 -0.12086890803236372 1104 44-45 -0.1803923398399192 1104 46-47 -0.17911207106365623 1104 48-49 -0.08674369022679684 1104 50-51 -0.08505465070316376 1104 52-53 -0.19935017394267618 1104 54-55 -0.37895004653612574 1104 56-57 -0.1967420254287262 1104 58-59 -0.25712435961565916 1104 60-61 -0.1479100182550468 1104 62-63 -0.45295164633313334 1104 64-65 -0.4421368722385566 1104 66-67 -0.13492669212808295 1104 68-69 -0.13197057892744368 1104 70-71 -0.5357790558622249 1104 72-73 -0.23418189280567958 1104 74-75 -0.10712604913287649 1104 76-77 -0.16861290663301176 1104 78-79 -0.07146912886691936 1104 80-81 -0.009211742048186977 1104 82-83 -0.14131934178368866 1104 84-85 -0.31432994562016603 1104 86-87 -0.1965078335658852 1104 88-89 0.1989313792739047 1104 90-91 -0.13539389146368208 1104 92-93 -0.26400058974394014 1104 94-95 -0.21663465393637082 1104 96-97 0.27514223743183663 1104 98-99 0.31327710099395034 1104 100-101 -0.21366620785585866 1105 1 0.020649269258036895 1105 2 0.1816981511704725 1105 3 0.12009227095585118 1105 4 0.11738936964260205 1105 5 0.1097117300976862 1105 6 0.05624067098545282 1105 7 0.04877484825479428 1105 8 0.11010981227215666 1105 9 0.15563574643735478 1105 10-11 0.16466694005456617 1105 12-13 0.11882790930287257 1105 14-15 0.36520052523794533 1105 16-17 0.2568591371535973 1105 18-19 0.15645272414627698 1105 20-21 0.08896454192091596 1105 22-23 0.09176567386591472 1105 24-25 0.1298399701497459 1105 26-27 0.1518176472427939 1105 28-29 0.15456528197628927 1105 30-31 0.03677624264788193 1105 32-33 -0.038922229321137536 1105 34-35 -0.25734847366496894 1105 36-37 -0.027286928904068475 1105 38-39 0.09289507839104516 1105 40-41 -0.08135819282066592 1105 42-43 -0.024118911333872006 1105 44-45 0.18379423491279567 1105 46-47 3.248239352231508E-4 1105 48-49 -0.20772542629113389 1105 50-51 0.09059343508319273 1105 52-53 -0.06258195292659963 1105 54-55 -0.0023603710082156226 1105 56-57 0.05183502513715865 1105 58-59 -0.08239277211912821 1105 60-61 0.03798198285426224 1105 62-63 -0.050009343466101086 1105 64-65 -0.12181279633031039 1105 66-67 0.15890081074423534 1105 68-69 0.08581133673886399 1105 70-71 -0.002219899463241859 1105 72-73 -0.052259623481958783 1105 74-75 0.21051192083661618 1105 76-77 -0.08895547097938206 1105 78-79 0.21500525804326642 1105 80-81 0.5713450240598625 1105 82-83 0.3340844324988943 1105 84-85 0.324633117121671 1105 86-87 0.45650248034138485 1105 88-89 0.2598111648080206 1105 90-91 0.19553594491364024 1105 92-93 0.3417453527041907 1105 94-95 0.4034517134817186 1105 96-97 -0.040649529930789186 1105 98-99 -0.3191225662141619 1105 100-101 -0.017485390931014422 1106 1 0.06732729310812147 1106 2 0.038119897707765915 1106 3 0.06653327532589515 1106 4 0.05790118358230956 1106 5 0.09762003318634527 1106 6 0.11871011769310513 1106 7 -0.017205449500920622 1106 8 0.02081272316765137 1106 9 0.1276230066403059 1106 10-11 -0.04964496276745223 1106 12-13 -0.07559102262809603 1106 14-15 0.040152380656060416 1106 16-17 0.09650434861941903 1106 18-19 0.11017652238344766 1106 20-21 0.00940006521548753 1106 22-23 0.1857735251361916 1106 24-25 0.19530919465164231 1106 26-27 0.3312117661969438 1106 28-29 0.2905181743546308 1106 30-31 0.05255470350262925 1106 32-33 0.112999850524794 1106 34-35 0.37353072046879277 1106 36-37 0.1868231370169795 1106 38-39 0.2776202839313697 1106 40-41 0.25105236935991826 1106 42-43 0.39257059314727627 1106 44-45 0.2536909092332209 1106 46-47 0.2132960853232717 1106 48-49 0.4224575231200163 1106 50-51 0.09847248107978146 1106 52-53 0.291086293869931 1106 54-55 0.4316667380948189 1106 56-57 0.7217446614603915 1106 58-59 0.12432154533883732 1106 60-61 0.23287865717468748 1106 62-63 0.06903183866855755 1106 64-65 0.3505577688130117 1106 66-67 0.14560920271519961 1106 68-69 0.1885889087705621 1106 70-71 0.30197793773400505 1106 72-73 -0.09282698886229923 1106 74-75 -0.012358607272414446 1106 76-77 -0.32944876777220955 1106 78-79 -0.2719494119811756 1106 80-81 0.06852671430503321 1106 82-83 0.009860300953082657 1106 84-85 0.05876577396190186 1106 86-87 0.21740352470846602 1106 88-89 -0.135429928444335 1106 90-91 0.0906677869994148 1106 92-93 0.3455376643848105 1106 94-95 0.5103623126965928 1106 96-97 0.7515325306573146 1106 98-99 0.8259666864337873 1106 100-101 0.6186897072098638 1107 1 -0.054021021498620314 1107 2 0.16471980000380881 1107 3 -0.07880136072686383 1107 4 -0.08892343781485579 1107 5 -0.05560419739499167 1107 6 -0.056375373269276 1107 7 -0.10689768203634031 1107 8 -0.04552826364231777 1107 9 -0.055083392968882094 1107 10-11 0.03314692780411832 1107 12-13 -0.10248403679516827 1107 14-15 0.01886513102244436 1107 16-17 0.03336316640153569 1107 18-19 0.12298795374641713 1107 20-21 0.04281481851299418 1107 22-23 0.17336512259588943 1107 24-25 0.19623738224323972 1107 26-27 0.05959476570842526 1107 28-29 0.058227016562746314 1107 30-31 0.1828551431704355 1107 32-33 0.07670283049548487 1107 34-35 0.077536582706216 1107 36-37 0.4687967569485849 1107 38-39 0.3998357211565775 1107 40-41 0.37605236935991826 1107 42-43 0.493755253626027 1107 44-45 0.4374109873963832 1107 46-47 0.5819453281176052 1107 48-49 0.5438546897052703 1107 50-51 0.6605511327651783 1107 52-53 0.786530846874328 1107 54-55 0.3748518870933424 1107 56-57 0.5180563370832516 1107 58-59 0.5872184676641865 1107 60-61 0.7736212072479631 1107 62-63 0.587436821570364 1107 64-65 0.4625142905521429 1107 66-67 0.5583839950942959 1107 68-69 0.3659215907441862 1107 70-71 0.4869193153597955 1107 72-73 0.8591026642886526 1107 74-75 0.6053380219410691 1107 76-77 0.4995693074598364 1107 78-79 0.5445747697481842 1107 80-81 0.6834021417598457 1107 82-83 0.9270806233761419 1107 84-85 0.6153974300439771 1107 86-87 0.8704201343811597 1107 88-89 0.7939056846479993 1107 90-91 0.8291997850453363 1107 92-93 0.8034761108918929 1107 94-95 0.7703525423008948 1107 96-97 1.0483327260652295 1107 98-99 1.2232065496482498 1107 100-101 0.7064278606050749 1108 1 -0.08353975891500198 1108 2 0.13916287332978072 1108 3 0.00895599460209695 1108 4 -0.03273716776224944 1108 5 0.2195727851340763 1108 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-1.362519125234968 2316 60-61 -0.8936853335572224 2316 62-63 -0.8005981190798366 2316 64-65 -0.3823132956547539 2316 66-67 -0.8335852909470631 2316 68-69 -0.8838794025283292 2316 70-71 -0.903634029009897 2316 72-73 -1.0118810981712834 2316 74-75 -1.076543560250471 2316 76-77 -1.3878104959990054 2316 78-79 -1.535834514884268 2316 80-81 -1.0815550629881407 2316 82-83 -0.8853352830730863 2316 84-85 -0.8171269614885546 2316 86-87 -1.2325385497088703 2316 88-89 -1.2604742244781342 2316 90-91 -1.9564188969313498 2316 92-93 -2.2495829571226196 2316 94-95 -2.5158473097649043 2316 96-97 -2.430864906987498 2316 98-99 -1.9243331636411725 2316 100-101 -1.6458598362002483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 16.0 6 21.0 7 58.0 8 127.0 9 238.0 10 419.0 11 669.0 12 1068.0 13 1471.0 14 1841.0 15 2679.0 16 3759.0 17 5071.0 18 4680.0 19 2837.0 20 2440.0 21 2522.0 22 2601.0 23 2761.0 24 3182.0 25 3802.0 26 4451.0 27 5665.0 28 6987.0 29 8575.0 30 10809.0 31 13285.0 32 17238.0 33 22813.0 34 31696.0 35 44511.0 36 65678.0 37 85472.0 38 47242.0 39 1995.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22843970017106 19.49940411077911 14.871290375471387 21.40086581357844 2 15.928956667286592 21.03134207100418 39.76292787016826 23.27677339154097 3 19.99985317975936 25.27118921947913 30.0156852957082 24.71327230505331 4 13.167025545659195 17.238426152490945 36.863071351668786 32.73147695018107 5 13.419957911222044 38.00910292174424 33.85919835560124 14.711740811432486 6 31.69261185958765 36.59153082347644 16.976769223993227 14.739088092942678 7 28.5175968542702 33.52614875660968 20.33321832921031 17.623036059909808 8 27.086672359834296 34.31536396714275 19.169662104791804 19.428301568231145 9 27.203791701127038 16.957683480065185 20.104384337944907 35.73414048086287 10-11 25.292525729050947 27.147757914466503 26.475964197101902 21.08375215938064 12-13 26.716982886449614 24.360138200361163 27.377031530627725 21.545847382561504 14-15 23.31359498874425 25.770407164529708 26.25073407066654 24.66526377605951 16-17 21.938533931868317 28.909533370282958 27.055567586614448 22.096365111234277 18-19 21.570774535017165 27.08021856211965 30.424473476219255 20.924533426643926 20-21 22.590046001761767 26.05742879514535 30.46845943036116 20.884065772731724 22-23 22.23482373782975 26.001820490798444 30.334294963553482 21.429060807818324 24-25 22.291673548587585 26.19681192719478 30.578729787033605 20.93278473718403 26-27 21.998996770089065 26.187236958011155 30.578447685230497 21.235318586669276 28-29 21.457881678175205 26.670981349802535 30.767197968102337 21.10393900391992 30-31 21.713698454160067 26.304359794703647 30.49751943769843 21.484422313437857 32-33 21.763589626260043 26.413224475213898 30.32864205898942 21.49454383953664 34-35 22.101955948042033 26.418206087210788 30.262444745415994 21.217393219331182 36-37 22.421903770323127 26.195285536185537 30.156467736864062 21.226342956627274 38-39 22.022774628569472 26.503554941619672 30.454061051274113 21.019609378536742 40-41 22.011735285625498 26.449904815969383 30.309017769316977 21.229342129088142 42-43 22.01381284753871 26.669317493622724 29.792073211762332 21.524796447076238 44-45 22.23922908736757 26.573899723118448 29.5653950670303 21.62147612248369 46-47 22.63153258523742 27.15791250899232 28.86596424603971 21.344590659730546 48-49 22.800419404685147 27.260203413719232 28.478532426172908 21.460844755422713 50-51 22.535483531554988 27.49724414602784 28.43833310291417 21.528939219503005 52-53 22.69679774689795 27.722844957313896 28.049872639050992 21.530484656737162 54-55 22.247031000913914 27.7182842873974 28.19873177523328 21.835952936455406 56-57 22.57122335433458 27.840093581791685 27.98998596517847 21.59869709869526 58-59 22.733023060057945 27.235900673400675 28.104323271474435 21.92675299506695 60-61 22.251150681110232 27.540980397434613 28.621433867340723 21.586435054114432 62-63 22.242232346910313 27.397096030654637 28.680000587253655 21.680671035181387 64-65 22.13899791517809 27.750888251588083 28.437019781338396 21.673094051895426 66-67 22.132284751602114 27.661930268009854 28.181041844577287 22.024743135810745 68-69 22.257825346488136 27.57822899538016 28.119127319708713 22.04481833842299 70-71 22.381399833114326 27.793193903044806 27.974272199832136 21.851134064008733 72-73 22.2479696381686 27.39110433576153 28.228942107465414 22.131983918604455 74-75 22.274603176545295 28.02216558608007 27.553061716401384 22.15016952097325 76-77 22.46755105761102 27.812190696644446 27.71125610348426 22.009002142260275 78-79 22.439670772427007 27.643468121525938 27.643223415436303 22.27363769061075 80-81 22.500452683557064 27.9408526111277 27.332299094143497 22.22639561117174 82-83 22.564064918301554 27.993295282839963 27.37041727076413 22.072222528094358 84-85 22.189718687411833 27.880202655665222 27.398032904017512 22.532045752905436 86-87 22.204169521385925 27.82360281883136 27.414970147792893 22.55725751198982 88-89 22.104606349788952 27.820150486327766 27.32782773597602 22.74741542790726 90-91 21.923227043422617 27.88901899318849 27.727632562618304 22.460121400770593 92-93 21.988947166870375 27.837381185714268 27.538242631342623 22.635429016072734 94-95 21.81846877378089 27.7717964465999 27.569681828525567 22.840052951093647 96-97 21.937131971963495 27.923089251654403 27.501611890922394 22.638166885459704 98-99 21.798675486965916 28.16296917992995 26.983313289052397 23.055042044051742 100-101 21.311222692036647 28.482499412731972 26.832153903374834 23.37412399185655 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 113.0 1 85.0 2 88.5 3 124.0 4 117.0 5 109.5 6 147.0 7 268.0 8 444.5 9 551.0 10 548.0 11 508.0 12 519.0 13 585.5 14 628.5 15 692.5 16 791.5 17 853.0 18 874.0 19 892.0 20 988.0 21 1054.0 22 1139.5 23 1318.0 24 1535.0 25 1841.5 26 2223.0 27 2711.0 28 3286.5 29 3928.0 30 4785.0 31 5656.0 32 6681.0 33 7700.5 34 8439.0 35 9292.5 36 10718.5 37 11748.5 38 12255.5 39 12630.5 40 12645.5 41 12887.5 42 13060.0 43 13387.5 44 13971.0 45 14346.5 46 14475.5 47 15157.0 48 17036.5 49 16812.0 50 14976.0 51 14084.5 52 13417.5 53 13186.5 54 12806.0 55 11871.5 56 10537.5 57 9438.5 58 8845.5 59 7896.0 60 6533.5 61 5366.0 62 4327.5 63 3298.5 64 2295.5 65 1605.5 66 1233.0 67 1017.5 68 824.5 69 639.0 70 489.5 71 339.5 72 255.5 73 195.5 74 151.5 75 131.0 76 103.5 77 67.5 78 45.0 79 31.5 80 26.0 81 22.0 82 15.5 83 9.0 84 2.0 85 1.5 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011989762211205778 2 0.007340670741554559 3 0.00464909146965122 4 4.893780494369705E-4 5 0.005383158543806676 6 0.0 7 2.4468902471848526E-4 8 0.0012234451235924263 9 0.0 10-11 0.0 12-13 0.0 14-15 4.893780494369705E-4 16-17 0.004282057932573493 18-19 0.002691579271903338 20-21 4.893780494369705E-4 22-23 2.4468902471848526E-4 24-25 6.117225617962132E-4 26-27 4.893780494369705E-4 28-29 0.0 30-31 0.0011011006112331837 32-33 0.0037926798831365216 34-35 0.001345789635951669 36-37 8.564115865146985E-4 38-39 0.006239570130321374 40-41 0.0 42-43 0.0011011006112331837 44-45 0.005750192080884404 46-47 0.0 48-49 0.0018351676853886397 50-51 0.0015904786606701544 52-53 2.4468902471848526E-4 54-55 1.2234451235924263E-4 56-57 0.013580240871875933 58-59 0.0024468902471848526 60-61 0.002691579271903338 62-63 0.0 64-65 0.003425646346058794 66-67 7.340670741554558E-4 68-69 0.0024468902471848526 70-71 0.00464909146965122 72-73 0.002691579271903338 74-75 0.007463015253913801 76-77 3.670335370777279E-4 78-79 0.006973637204476831 80-81 0.001957512197747882 82-83 0.0035479908584180363 84-85 0.0020798567101071247 86-87 4.893780494369705E-4 88-89 0.001712823173029397 90-91 0.008686460377506228 92-93 0.0020798567101071247 94-95 7.340670741554558E-4 96-97 1.2234451235924263E-4 98-99 8.564115865146985E-4 100-101 0.0024468902471848526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 408682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.8269407828322 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09114357569217 60.81308125168993 2 7.845604444972273 10.95669113969208 3 2.046518798719006 4.287064409073146 4 0.9019628929756265 2.519252380644843 5 0.5246659721186177 1.83179098829469 6 0.3159750696514077 1.3238143486440075 7 0.22284355565229055 1.089233863505778 8 0.16253034616265027 0.9079197485530078 9 0.11971470460491768 0.7523380430353651 >10 0.6964953567546109 8.879169416261167 >50 0.051767750285716424 2.5182992642855595 >100 0.019016724579293747 2.4187082851556165 >500 3.5216156628321756E-4 0.14901761089917684 >1k 0.0014086462651328702 1.5536192502656754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2172 0.5314645616885499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1933 0.47298388478083203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1163 0.28457333574759835 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1050 0.2569234759544095 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.14828154897940207 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 422 0.10325876843120078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4468902471848526E-4 0.0 10-11 0.0 0.0 0.0 2.4468902471848526E-4 0.0 12-13 2.4468902471848526E-4 0.0 0.0 4.893780494369705E-4 0.0 14-15 2.4468902471848526E-4 0.0 0.0 4.893780494369705E-4 0.0 16-17 2.4468902471848526E-4 0.0 0.0 6.117225617962132E-4 0.0 18-19 2.4468902471848526E-4 0.0 0.0 9.78756098873941E-4 0.0 20-21 2.4468902471848526E-4 0.0 0.0 0.0011011006112331837 0.0 22-23 2.4468902471848526E-4 0.0 0.0 0.0012234451235924263 0.0 24-25 2.4468902471848526E-4 0.0 0.0 0.0017128231730293968 0.0 26-27 2.4468902471848526E-4 0.0 0.0 0.0022022012224663674 0.0 28-29 2.4468902471848526E-4 0.0 0.0 0.004404402444932735 0.0 30-31 2.4468902471848526E-4 0.0 0.0 0.008074737815710014 0.0 32-33 2.4468902471848526E-4 0.0 0.0 0.011989762211205778 0.0 34-35 2.4468902471848526E-4 0.0 0.0 0.015537753069623815 0.0 36-37 2.4468902471848526E-4 0.0 0.0 0.02214435673702292 0.0 38-39 2.4468902471848526E-4 0.0 0.0 0.03401177443586945 0.0 40-41 2.4468902471848526E-4 0.0 0.0 0.04746967079538614 0.0 42-43 2.4468902471848526E-4 0.0 0.0 0.06924699399533132 0.0 44-45 2.4468902471848526E-4 0.0 0.0 0.08845508243573243 0.0 46-47 2.4468902471848526E-4 0.0 0.0 0.12907346053900098 0.0 48-49 2.4468902471848526E-4 0.0 0.0 0.17666547584674636 0.0 50-51 2.4468902471848526E-4 0.0 0.0 0.19305964050288488 0.0 52-53 2.4468902471848526E-4 0.0 0.0 0.22046481127135525 0.0 54-55 2.4468902471848526E-4 0.0 0.0 0.27759969854312155 0.0 56-57 2.4468902471848526E-4 0.0 0.0 0.3623844456080767 0.0 58-59 2.4468902471848526E-4 0.0 0.0 0.46319632379209263 0.0 60-61 2.4468902471848526E-4 0.0 0.0 0.5826045678547135 0.0 62-63 2.4468902471848526E-4 0.0 0.0 0.7086194155847334 0.0 64-65 2.4468902471848526E-4 0.0 0.0 0.832921440141724 0.0 66-67 2.4468902471848526E-4 0.0 0.0 1.001879211709838 0.0 68-69 2.4468902471848526E-4 0.0 0.0 1.2290729711609516 0.0 70-71 2.4468902471848526E-4 0.0 0.0 1.3558218859651268 0.0 72-73 2.4468902471848526E-4 0.0 0.0 1.516337886180453 0.0 74-75 2.4468902471848526E-4 0.0 0.0 1.685540346773286 0.0 76-77 2.4468902471848526E-4 0.0 0.0 1.874684962880675 0.0 78-79 2.4468902471848526E-4 0.0 0.0 2.0765534082734254 0.0 80-81 2.4468902471848526E-4 0.0 0.0 2.407250625180458 0.0 82-83 2.4468902471848526E-4 0.0 0.0 2.752506839058241 0.0 84-85 2.4468902471848526E-4 0.0 0.0 3.294003650760249 0.0 86-87 2.4468902471848526E-4 0.0 0.0 3.9519724382282555 0.0 88-89 2.4468902471848526E-4 0.0 0.0 4.640405009273714 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGA 165 0.0 37.423424 1 GTAAGGT 155 3.6379788E-11 33.70069 4 TACCTGG 280 0.0 27.142263 2 TAGGACC 185 8.956704E-9 25.66883 4 CTAGGAC 225 1.0186341E-10 25.329678 3 GTACATG 2115 0.0 25.153112 1 TAAGGTG 190 1.1896191E-8 24.993336 5 GTATCAA 3530 0.0 24.893223 1 GGTATCA 2595 0.0 24.710442 1 TACATGG 2125 0.0 24.587698 2 ACATGGG 2065 0.0 24.149057 3 GGTAAGG 160 1.8570026E-6 23.746574 3 GGTAGTA 140 1.7795115E-5 23.743668 5 GAGTACT 2115 0.0 23.13188 12-13 CTGGTAC 165 2.4265573E-6 23.02698 3 CTAACAC 165 2.4265573E-6 23.02698 3 GTACTTT 2215 0.0 22.087553 14-15 ATGGGAG 590 0.0 21.731493 5 ATGGGAC 285 8.367351E-11 21.66089 5 TAGTCGG 55 0.002877028 21.590435 40-41 >>END_MODULE