FastQCFastQC Report
Thu 26 May 2016
SRR936526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551155
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.38101804392593736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17130.3108018615453003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.18325153541199846No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6960.1262802659868821No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5870.10650361513548821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5500.029.3663852
GTACATG25800.028.5420841
ACATGGG25850.027.7492143
GTTACAC1405.792135E-727.14543
GTACCTG6150.027.0375061
TACATGG26800.026.942822
GTATCAA37550.026.1899151
GTGTAGC2600.025.581641
GGTATCA27200.025.1516971
GCCGCGC950.001224427425.0046088
GAGTACT20450.024.27709212-13
CATGGGG11000.023.7522224
TCATACC1252.2524052E-422.8021354
ATGGGGA6050.022.7748585
TCAACGC43200.022.542624
TAGTCCA1703.1390246E-622.3570639
TATCAAC44200.022.3550342
ATCAACG43650.022.3102233
CAACGCA43500.022.2819925
CATGGGA14750.022.2224184
Produced by FastQC (version 0.11.5)