##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 551155 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51834239007176 31.0 31.0 34.0 30.0 34.0 2 31.588712794041605 33.0 31.0 34.0 30.0 34.0 3 31.624367011094883 33.0 31.0 34.0 30.0 34.0 4 35.26334878573178 37.0 35.0 37.0 33.0 37.0 5 35.205116528018436 37.0 35.0 37.0 33.0 37.0 6 35.303921764294984 37.0 35.0 37.0 33.0 37.0 7 35.14372544928378 37.0 35.0 37.0 32.0 37.0 8 35.1261224156544 37.0 35.0 37.0 32.0 37.0 9 36.68970434814163 39.0 37.0 39.0 32.0 39.0 10-11 36.55214322649708 39.0 37.0 39.0 32.0 39.0 12-13 36.48044107374514 39.0 37.0 39.0 32.0 39.0 14-15 37.599211655523405 40.0 37.0 41.0 32.0 41.0 16-17 37.52255717538623 40.0 37.0 41.0 32.0 41.0 18-19 37.48674782955793 40.0 37.0 41.0 32.0 41.0 20-21 37.38501419745806 40.0 37.0 41.0 32.0 41.0 22-23 37.28626883544557 39.5 37.0 41.0 32.0 41.0 24-25 37.14334443123985 39.0 36.5 41.0 31.0 41.0 26-27 36.94630276419519 39.0 36.0 41.0 31.0 41.0 28-29 37.043687347479384 39.0 36.0 41.0 31.0 41.0 30-31 36.983899266086674 39.0 36.0 41.0 31.0 41.0 32-33 36.828275167602584 39.0 36.0 41.0 30.0 41.0 34-35 36.650513920766386 39.0 35.5 41.0 30.0 41.0 36-37 36.41364498190164 39.0 35.0 40.5 30.0 41.0 38-39 36.244619027315366 39.0 35.0 40.0 30.0 41.0 40-41 36.10152951529062 39.0 35.0 40.0 29.5 41.0 42-43 35.83096497355554 38.0 35.0 40.0 28.5 41.0 44-45 35.55017100452686 38.0 35.0 40.0 27.5 41.0 46-47 35.20396349484265 38.0 34.5 40.0 26.5 41.0 48-49 34.95468334678992 38.0 34.0 40.0 25.0 41.0 50-51 34.30087634150103 37.5 33.5 39.5 24.5 40.5 52-53 34.502023024376086 38.0 34.0 39.5 25.0 40.5 54-55 34.85393219693189 38.0 34.0 40.0 25.5 41.0 56-57 34.78876903956237 38.0 34.0 40.0 25.0 41.0 58-59 34.55616205967468 38.0 34.0 40.0 25.0 41.0 60-61 34.23687710353712 38.0 34.0 40.0 23.5 41.0 62-63 33.931063856809786 37.0 33.0 40.0 23.0 41.0 64-65 33.618554671553376 37.0 33.0 40.0 22.0 41.0 66-67 33.244256152987816 36.0 33.0 39.0 22.0 41.0 68-69 32.859974054485576 36.0 32.0 39.0 20.5 41.0 70-71 32.43765456178389 35.0 32.0 39.0 19.5 40.0 72-73 32.01825167148987 35.0 31.5 38.0 18.0 40.0 74-75 31.58763142854551 35.0 31.0 37.0 17.0 39.5 76-77 31.160029392820533 35.0 31.0 37.0 13.0 39.0 78-79 30.700469922254175 35.0 30.5 36.0 9.0 39.0 80-81 30.28386751458301 34.0 30.0 36.0 7.0 37.5 82-83 29.8626910760131 34.0 30.0 35.0 4.5 37.0 84-85 29.47396739574167 34.0 29.0 35.0 2.0 37.0 86-87 29.131461204198455 34.0 29.0 35.0 2.0 36.0 88-89 28.74330179350636 34.0 29.0 35.0 2.0 36.0 90-91 28.386010287487185 34.0 29.0 35.0 2.0 35.5 92-93 28.012541843945897 34.0 27.5 35.0 2.0 35.0 94-95 27.588575808982952 34.0 27.0 35.0 2.0 35.0 96-97 27.008465858061708 33.0 25.5 35.0 2.0 35.0 98-99 26.3375348132558 33.0 25.0 35.0 2.0 35.0 100-101 24.302700692182782 31.0 12.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2793762603685366 1101 2 0.3560349544232544 1101 3 0.34511919223968945 1101 4 0.36489317501334284 1101 5 0.4197577694907295 1101 6 0.2963612494998529 1101 7 0.33180648602096596 1101 8 0.30210958561416135 1101 9 0.455224482917842 1101 10-11 0.31633002134481814 1101 12-13 0.42714558068787767 1101 14-15 0.5775266852194747 1101 16-17 0.6301572425121265 1101 18-19 0.5988551141806724 1101 20-21 0.5506689063503316 1101 22-23 0.5929523354501072 1101 24-25 0.544026791878693 1101 26-27 0.6639951021892969 1101 28-29 0.6374528580469203 1101 30-31 0.5942972624734395 1101 32-33 0.5593070718448487 1101 34-35 0.4577990775454097 1101 36-37 0.6343261716950224 1101 38-39 0.6823318420951594 1101 40-41 0.776085548422806 1101 42-43 0.48569392627749153 1101 44-45 0.5084496751548571 1101 46-47 0.6816811343077234 1101 48-49 0.21483925339253318 1101 50-51 0.5914626279356483 1101 52-53 0.6033247385539511 1101 54-55 0.7089798651774473 1101 56-57 0.8117426978568005 1101 58-59 0.9158549473694748 1101 60-61 0.947679318039448 1101 62-63 1.0176685602295308 1101 64-65 0.8503760379814693 1101 66-67 0.7477200330739251 1101 68-69 0.8583521688110665 1101 70-71 0.82483526603977 1101 72-73 0.7117659753224821 1101 74-75 0.8527463705583678 1101 76-77 0.8595077632802877 1101 78-79 0.7947958032255578 1101 80-81 0.6691974393401807 1101 82-83 0.6284416320090962 1101 84-85 0.5626116521717535 1101 86-87 0.7363741298795539 1101 88-89 0.7437443020565802 1101 90-91 0.6041401020897297 1101 92-93 0.6617801427960188 1101 94-95 0.5233443339482164 1101 96-97 0.03444652771390011 1101 98-99 -0.15587725797548657 1101 100-101 0.15976849439021024 1102 1 0.20762979886776023 1102 2 0.060888471927668775 1102 3 0.10491239342239567 1102 4 0.2582745631197909 1102 5 0.28936363578882407 1102 6 0.2284211896646653 1102 7 0.21051119410572028 1102 8 0.0188087650672486 1102 9 0.420646478473941 1102 10-11 0.3548259623494232 1102 12-13 0.2284576465765653 1102 14-15 0.40523315599295273 1102 16-17 0.2524011277755207 1102 18-19 0.13294761378701736 1102 20-21 -0.0645187565780958 1102 22-23 -0.2392456018104241 1102 24-25 -0.00453407993053645 1102 26-27 0.08815902786768248 1102 28-29 0.08146452765869583 1102 30-31 0.09336124413797364 1102 32-33 0.07803782660804615 1102 34-35 0.025825522250343624 1102 36-37 0.005536718959575637 1102 38-39 -0.1350751465501432 1102 40-41 -0.22571650556882616 1102 42-43 0.12694795028155426 1102 44-45 0.29210423627598203 1102 46-47 0.4364022314611731 1102 48-49 0.4289949998452869 1102 50-51 0.5702846663561374 1102 52-53 0.4393629753403516 1102 54-55 0.27240549545894766 1102 56-57 0.43871041397206056 1102 58-59 0.4379394033416233 1102 60-61 -0.03893321410884454 1102 62-63 0.019177561751654082 1102 64-65 0.3508167101651125 1102 66-67 0.20549150026079843 1102 68-69 0.25052198178235585 1102 70-71 0.25179357823250825 1102 72-73 0.08013347624099865 1102 74-75 -0.23430661507768846 1102 76-77 0.20568419398868798 1102 78-79 0.16799648293233105 1102 80-81 0.24447037178939723 1102 82-83 0.22200519377755867 1102 84-85 0.18925679187467637 1102 86-87 0.0375609923810849 1102 88-89 -0.10570217868943388 1102 90-91 -0.25164637200793294 1102 92-93 -0.3075696306350544 1102 94-95 -0.036803591940127944 1102 96-97 -0.2246758238790818 1102 98-99 -0.5797667203335521 1102 100-101 -0.3952111012848576 1103 1 0.22741490205472203 1103 2 0.3026098958752428 1103 3 0.2818873421225909 1103 4 0.4129025426948445 1103 5 0.38799547440876125 1103 6 0.288750008282058 1103 7 0.18865660311698917 1103 8 0.1607161429912125 1103 9 0.3729645297562456 1103 10-11 0.41805718762115873 1103 12-13 0.4954271966129298 1103 14-15 0.5342743432990957 1103 16-17 0.6123732846666954 1103 18-19 0.33626729216661744 1103 20-21 0.29722921079998343 1103 22-23 0.13964437292083431 1103 24-25 0.2883915459770492 1103 26-27 0.2947035331026413 1103 28-29 0.2665512187026593 1103 30-31 0.10483590415962141 1103 32-33 0.26283458940925186 1103 34-35 0.26883537731121265 1103 36-37 0.36610310377932365 1103 38-39 0.33170245099446305 1103 40-41 0.3141417545118017 1103 42-43 0.41624135016508035 1103 44-45 0.4286048273796794 1103 46-47 0.5274803146355893 1103 48-49 0.8126144290365644 1103 50-51 0.31328639842745076 1103 52-53 0.6696303591628521 1103 54-55 0.8384441508917249 1103 56-57 0.8115231428216703 1103 58-59 1.049856703809759 1103 60-61 1.2124626904047844 1103 62-63 1.0744601293161864 1103 64-65 0.9377589419627412 1103 66-67 0.8382498925587072 1103 68-69 0.7611624732607183 1103 70-71 0.8648674674449239 1103 72-73 0.8235194882030363 1103 74-75 0.8420613588487633 1103 76-77 0.6167530794395404 1103 78-79 0.6776265994784865 1103 80-81 0.8603566899256592 1103 82-83 0.7799346062479735 1103 84-85 0.901238701352078 1103 86-87 0.7119303359685496 1103 88-89 0.8194176041643111 1103 90-91 0.6871319053684175 1103 92-93 0.6919323676203781 1103 94-95 0.4662600248147264 1103 96-97 0.8170138579246746 1103 98-99 0.6046613837106953 1103 100-101 0.8698826630084753 1104 1 0.20441304052826936 1104 2 0.11378505950942497 1104 3 0.29316458946541246 1104 4 0.2380121325585307 1104 5 0.2705091356109506 1104 6 -8.224411264592391E-4 1104 7 0.3729267046002036 1104 8 0.23551647926696262 1104 9 0.30008576917256846 1104 10-11 0.4497736951573472 1104 12-13 0.2199813310031331 1104 14-15 0.3913481438155273 1104 16-17 0.07650287092743469 1104 18-19 0.38040198261278846 1104 20-21 0.23222891055377204 1104 22-23 0.6498617511710023 1104 24-25 0.551758506884994 1104 26-27 0.5430566826852967 1104 28-29 0.3460699240410392 1104 30-31 0.2501190363279946 1104 32-33 0.3406989381752439 1104 34-35 0.4439329573268296 1104 36-37 0.6106266130569367 1104 38-39 0.4787510308299261 1104 40-41 0.3565605379141985 1104 42-43 0.16761647776971955 1104 44-45 0.36013368103967736 1104 46-47 0.8406896462875437 1104 48-49 0.44204606297639515 1104 50-51 0.376558781781803 1104 52-53 0.6932522290962027 1104 54-55 0.9306512607217954 1104 56-57 0.7433132106773144 1104 58-59 0.6833386590130246 1104 60-61 0.7741137442689521 1104 62-63 0.5403881903262118 1104 64-65 0.4949168114156919 1104 66-67 0.655877347071403 1104 68-69 0.7862210212700802 1104 70-71 0.8694522269477147 1104 72-73 0.4435519147928453 1104 74-75 0.2724552818656356 1104 76-77 0.2898330049784832 1104 78-79 0.49635896001412405 1104 80-81 0.6946913443422815 1104 82-83 0.7209016992642496 1104 84-85 0.8544963726425365 1104 86-87 0.4943974590094413 1104 88-89 0.6907020574159795 1104 90-91 0.655984364384814 1104 92-93 0.49141444292212455 1104 94-95 0.7748470661886557 1104 96-97 0.980539949319617 1104 98-99 0.7155182863708767 1104 100-101 1.0794064725322876 1105 1 0.3821259198170779 1105 2 0.46945326904582885 1105 3 0.26016921807462623 1105 4 0.2723336372238734 1105 5 0.2867460028506841 1105 6 0.39931347835043596 1105 7 0.46237274089438074 1105 8 0.40588970680351366 1105 9 0.6101695249583301 1105 10-11 0.6109686845042006 1105 12-13 0.47246094308160025 1105 14-15 0.4647850698312439 1105 16-17 0.5434560705453819 1105 18-19 0.5444549544737782 1105 20-21 0.4745072148664633 1105 22-23 0.3226043858047518 1105 24-25 0.31577658050192525 1105 26-27 0.3379082733105321 1105 28-29 0.38373614988778115 1105 30-31 0.46749468837413133 1105 32-33 0.39457264326671293 1105 34-35 0.38557574624755375 1105 36-37 0.3244903997000961 1105 38-39 0.2731930262747895 1105 40-41 0.23489056509809103 1105 42-43 0.17854146491246325 1105 44-45 0.34075597183389306 1105 46-47 0.5613475700256956 1105 48-49 0.6842600810500059 1105 50-51 0.5972828285087175 1105 52-53 0.6421446690344794 1105 54-55 0.7681227911016322 1105 56-57 0.6121545889742777 1105 58-59 0.6769008513574448 1105 60-61 0.6938128846701019 1105 62-63 0.6597254910388699 1105 64-65 0.5527781000792729 1105 66-67 0.5006426104613055 1105 68-69 0.6239001043646368 1105 70-71 0.6993728793612064 1105 72-73 0.5707615246672084 1105 74-75 0.39801916401977877 1105 76-77 0.46861487258556167 1105 78-79 0.23705031259659393 1105 80-81 0.38678046343713035 1105 82-83 0.21877290637614166 1105 84-85 0.5015134911496304 1105 86-87 0.4075394030253108 1105 88-89 0.20133683810806602 1105 90-91 0.12045427842492984 1105 92-93 0.23717706139247596 1105 94-95 0.12981363734433415 1105 96-97 0.4286040151487249 1105 98-99 0.22200568626801953 1105 100-101 0.5392441038598896 1106 1 0.2666931006688671 1106 2 0.1344685492895863 1106 3 0.10941474184868838 1106 4 0.32153238937514317 1106 5 0.45064648373561766 1106 6 0.28857371475147175 1106 7 0.4571052571622758 1106 8 0.5984548351530137 1106 9 0.6429664225920533 1106 10-11 0.5259033631649856 1106 12-13 0.5347137459732707 1106 14-15 0.3765649344205855 1106 16-17 0.33104592428189505 1106 18-19 0.19267899508789554 1106 20-21 0.3755443642495777 1106 22-23 0.4141166985479714 1106 24-25 0.46281543727631913 1106 26-27 0.5209113293592864 1106 28-29 0.36120971029809823 1106 30-31 0.49374128834961084 1106 32-33 0.3010884298246381 1106 34-35 0.3354279388376611 1106 36-37 0.2833926742540598 1106 38-39 0.5448589588237454 1106 40-41 0.20560153917389812 1106 42-43 0.5608892641486776 1106 44-45 0.5032317345973283 1106 46-47 0.6224310823475179 1106 48-49 0.5437862799701776 1106 50-51 0.8392340225473802 1106 52-53 0.7443463410064766 1106 54-55 0.8283787181560669 1106 56-57 1.117655930820348 1106 58-59 1.061985698713947 1106 60-61 0.8621089899108014 1106 62-63 0.7606409433840398 1106 64-65 0.7357209887491862 1106 66-67 1.0784117856914222 1106 68-69 0.8078533509055887 1106 70-71 0.5190194584483834 1106 72-73 0.39004709948136806 1106 74-75 0.5066053895498186 1106 76-77 0.45364908852515384 1106 78-79 0.8416371102843492 1106 80-81 0.7894625662529648 1106 82-83 0.787522650948592 1106 84-85 0.796324565580079 1106 86-87 1.03862822824021 1106 88-89 1.0621515271221824 1106 90-91 1.1126404658111184 1106 92-93 0.9991219889418694 1106 94-95 1.0906685136265821 1106 96-97 1.3231544399531252 1106 98-99 1.6360026620058115 1106 100-101 1.8720900435835297 1107 1 0.10569509181031833 1107 2 0.3894260851504505 1107 3 0.44636971767054234 1107 4 0.4745505940969963 1107 5 0.3826886227904325 1107 6 0.19469037938635836 1107 7 0.39023439690789985 1107 8 0.5476959664464474 1107 9 0.5776498717366749 1107 10-11 0.6879147207983749 1107 12-13 0.6526736386954397 1107 14-15 0.7009635284309113 1107 16-17 0.7124003068248683 1107 18-19 0.8903378800486834 1107 20-21 1.1033827567076244 1107 22-23 0.910759187068436 1107 24-25 0.9591303017567938 1107 26-27 0.9074797596083712 1107 28-29 0.49863402660513856 1107 30-31 0.7225549337638952 1107 32-33 0.8544809894572936 1107 34-35 1.0285483419422121 1107 36-37 0.7654343053646286 1107 38-39 0.3851612872401802 1107 40-41 0.5902143840680409 1107 42-43 0.825949811103051 1107 44-45 0.6453900912960933 1107 46-47 0.4785101591080547 1107 48-49 0.907751191181525 1107 50-51 0.4239946792176994 1107 52-53 0.5169701778141444 1107 54-55 0.7219974145679444 1107 56-57 0.7404285952926983 1107 58-59 0.52628737696174 1107 60-61 0.6180240006792133 1107 62-63 0.47981126724928913 1107 64-65 0.30292963192851374 1107 66-67 0.2847235009175577 1107 68-69 0.5307723033213705 1107 70-71 0.1678496628451569 1107 72-73 0.13553909428002342 1107 74-75 -0.07978830787795133 1107 76-77 0.19944838959386857 1107 78-79 0.17616665232181816 1107 80-81 0.6668067289576634 1107 82-83 0.7010299043924526 1107 84-85 0.6657143213604861 1107 86-87 0.6443974590094435 1107 88-89 0.6939071856211037 1107 90-91 0.8460484669489148 1107 92-93 0.6705811095887917 1107 94-95 0.7953598867014762 1107 96-97 0.9218861031657717 1107 98-99 0.7559029017554941 1107 100-101 1.3412654468912635 1108 1 0.37318227129749815 1108 2 0.2590158287401927 1108 3 -0.05114310284227841 1108 4 -0.06942376487736368 1108 5 -0.012157531055720483 1108 6 0.09645961015559124 1108 7 0.4322600379335384 1108 8 0.305721607472087 1108 9 0.42013705122385403 1108 10-11 0.32774805413170327 1108 12-13 0.5276223566441587 1108 14-15 0.716271220738598 1108 16-17 0.33716953759409307 1108 18-19 0.45699172620252426 1108 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-4.4144902410827385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1318.0 3 511.0 4 476.0 5 542.0 6 596.0 7 683.0 8 833.0 9 1022.0 10 1260.0 11 1646.0 12 1980.0 13 2485.0 14 2775.0 15 3338.0 16 4116.0 17 5351.0 18 4812.0 19 3261.0 20 3287.0 21 3583.0 22 4014.0 23 4425.0 24 4922.0 25 5860.0 26 6832.0 27 8066.0 28 9739.0 29 11537.0 30 14166.0 31 17487.0 32 22178.0 33 28656.0 34 38413.0 35 53718.0 36 76900.0 37 102411.0 38 84089.0 39 13860.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55247199207395 18.588298910893606 14.413693489676897 22.445535607355552 2 15.332372878895647 19.71629095237404 41.17809292941023 23.773243239320085 3 19.473819823481595 24.812739519219605 29.789114214376962 25.924326442921846 4 12.509029660847753 16.106010940863435 37.709497206703915 33.6754621915849 5 13.446845587634668 37.298759291698055 34.55631290899355 14.69808221167373 6 31.58074372348286 35.520303255310694 18.202762048788813 14.696190972417636 7 28.59862646736408 32.559148293610754 20.953087907540635 17.889137331484534 8 26.47134622644249 34.01356099544095 20.19067427476261 19.32441850335395 9 26.52926050734303 16.69107386216195 20.687321142438215 36.0923444880568 10-11 24.901275096455887 26.368511861056326 27.526254788759115 21.203958253728675 12-13 25.90495119593776 23.93354842515576 27.995062871248937 22.16643750765754 14-15 22.966127300954454 25.096883486662247 26.63712903673633 25.29986017564697 16-17 21.880376314815216 28.455813497680698 27.279648221143027 22.384161966361056 18-19 21.684348267185 26.869226937013213 29.90018992861116 21.546234867190623 20-21 22.618451330966845 25.930261257141197 29.720268298525664 21.73101911336629 22-23 22.079199386656143 25.96794461759008 29.83246458075316 22.12039141500061 24-25 22.124162757341832 26.132494586216374 29.902592719266174 21.840749937175616 26-27 21.801459514970734 26.22264316637092 29.93221347674465 22.0436838419137 28-29 21.59461416059904 26.38442599006802 30.11100446521721 21.90995538411573 30-31 21.66858246064155 26.148909358298226 29.93601217882478 22.246496002235446 32-33 21.582930916077974 26.22959148033606 29.904735293664046 22.282742309921918 34-35 22.187788886963606 26.262121345452332 29.535304198256178 22.014785569327884 36-37 22.110047629848037 26.022408709457928 29.732093445225676 22.13545021546836 38-39 21.767262691574572 26.337018835161263 29.86780639911018 22.027912074153978 40-41 21.775174662446283 26.267090882534117 29.861131901559418 22.096602553460183 42-43 21.794390285057997 26.28889360628938 29.480940794809406 22.435775313843212 44-45 21.81701565194672 26.482026978356892 29.24463685179322 22.456320517903166 46-47 22.119344083826633 26.651334739516003 28.93062076160668 22.29870041505069 48-49 22.13031396879454 26.720323829931225 28.695817045523615 22.45354515575062 50-51 21.997559636939297 26.99340920533977 28.75872611233835 22.250305045382586 52-53 22.18890813143029 26.989765971788156 28.47139125993488 22.349934636846676 54-55 21.940162697804297 26.901565741833583 28.566737035387273 22.59153452497485 56-57 22.028927130593033 27.263135644346708 28.246001268272238 22.46193595678802 58-59 22.234056354460282 26.803160378599493 28.352685768407643 22.61009749853258 60-61 22.047939849296654 26.872950871724342 28.82587104394637 22.253238235032633 62-63 21.87946508355688 26.91229181087233 28.911923491090924 22.296319614479867 64-65 21.829398289900436 27.01612573994693 28.631512099975055 22.522963870177588 66-67 21.70121737142484 27.167731265643525 28.346637418544795 22.784413944386834 68-69 21.729908233575713 27.019519076801647 28.566348077350522 22.684224612272114 70-71 21.971004236865262 26.926684993150253 28.39343875597652 22.708872014007966 72-73 21.745717673549777 26.938035659366232 28.42497661628849 22.8912700507955 74-75 21.668967944798357 27.397336081561406 28.28340376272583 22.650292210914408 76-77 21.82544938591032 27.188686742593998 28.28268991056735 22.70317396092833 78-79 21.75612075803199 27.05866017609487 28.266748706332486 22.91847035954065 80-81 21.884292181342545 27.223340825302227 28.144063574966722 22.748303418388506 82-83 21.98012454366649 27.216619611998578 28.183084995971925 22.620170848363006 84-85 21.724010916032196 27.090394762627106 28.06205953011707 23.123534791223626 86-87 21.91484786874716 26.92274542998388 28.29604105824599 22.866365643022974 88-89 21.934108404058918 26.788204288442437 28.160832467691527 23.116854839807125 90-91 21.53940789491591 26.899763473697675 28.498289335086184 23.062539296300226 92-93 21.615069359446977 27.015933301698396 28.523341048507607 22.84565629034702 94-95 21.39460238200649 26.89872843529318 28.4870956504068 23.219573532293534 96-97 21.36274563170214 26.85373686788961 28.57383920309183 23.209678297316422 98-99 21.298356478624786 27.095417159698883 28.143670192300714 23.462556169375624 100-101 21.211587647055623 27.020568273198077 28.34949182878106 23.418352250965242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 45.5 2 79.0 3 111.5 4 102.0 5 101.0 6 154.0 7 281.0 8 451.0 9 548.0 10 550.5 11 528.0 12 545.5 13 591.0 14 629.5 15 719.0 16 787.0 17 808.0 18 864.0 19 938.0 20 1007.5 21 1151.5 22 1324.5 23 1581.5 24 1898.5 25 2251.0 26 2797.0 27 3515.5 28 4193.5 29 4990.5 30 6028.0 31 7292.5 32 9034.5 33 10487.5 34 11672.5 35 12802.5 36 14473.0 37 16025.5 38 16661.5 39 17066.0 40 17166.5 41 17496.5 42 17612.5 43 18105.5 44 19106.5 45 19967.0 46 20477.5 47 21016.0 48 23018.0 49 23028.0 50 20621.5 51 19223.0 52 18429.0 53 18012.0 54 17747.5 55 16348.0 56 14373.5 57 12803.5 58 11806.5 59 10517.5 60 8708.5 61 7243.5 62 5914.5 63 4527.5 64 3209.0 65 2360.5 66 1705.0 67 1285.0 68 1053.5 69 815.5 70 622.5 71 443.5 72 339.5 73 280.0 74 197.5 75 143.0 76 111.5 77 69.0 78 40.0 79 27.5 80 17.5 81 12.5 82 10.0 83 9.5 84 8.5 85 5.0 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011067666990229609 2 0.004898803421904909 3 0.00925329535248705 4 0.03501737260843138 5 0.04717366258130653 6 0.06023713837305295 7 0.03030000635030073 8 0.029574257695203707 9 0.015966470412134517 10-11 0.04635719534432237 12-13 0.041276954758643215 14-15 0.019323057941958252 16-17 0.0230425197993305 18-19 0.028757790458219558 20-21 0.0230425197993305 22-23 0.01406138019250483 24-25 0.002812276038500966 26-27 0.0023586831290653264 28-29 0.0010886229826455353 30-31 0.006259582150211828 32-33 0.0011793415645326632 34-35 0.0017236530558554306 36-37 0.005443114913227676 38-39 0.00571527065888906 40-41 0.003719461857372245 42-43 7.257486550970235E-4 44-45 0.0017236530558554306 46-47 0.0031751503660494783 48-49 2.721557456613838E-4 50-51 0.001360778728306919 52-53 6.350300732098956E-4 54-55 0.0019050902196296867 56-57 0.0020865273834039426 58-59 0.003356587529823734 60-61 0.0010886229826455353 62-63 6.350300732098956E-4 64-65 0.0031751503660494783 66-67 0.0015422158920811752 68-69 0.004989522003792037 70-71 0.007257486550970234 72-73 0.004263773348695013 74-75 0.0021772459652910706 76-77 0.0025401202928395823 78-79 0.03665030708239969 80-81 0.021863178234797834 82-83 0.004173054766807885 84-85 0.0070760493871959785 86-87 0.006441019313986084 88-89 0.004082336184920757 90-91 0.009706888261922689 92-93 0.013879943028730576 94-95 0.004173054766807885 96-97 0.004535929094356397 98-99 0.02594551441971859 100-101 0.04681078825375802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 551155.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.46194137899124 #Duplication Level Percentage of deduplicated Percentage of total 1 87.53051111690039 62.551002543090775 2 7.69974177189541 11.0047699027312 3 1.9299594620295561 4.137559498181568 4 0.8326645963143576 2.380153142807121 5 0.4673004362410433 1.6697098190517234 6 0.2878288070264524 1.2341283200945594 7 0.2182463885250964 1.0917417436069892 8 0.15239141438331397 0.8712149057057558 9 0.126415986810833 0.8130538654958769 >10 0.6912280800805536 8.906259128251778 >50 0.043151522382637274 2.06353356751643 >100 0.019291255841857053 2.169284857210909 >500 5.076646274160354E-4 0.23272785950305863 >1k 7.61496941124053E-4 0.8748608467523397 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.38101804392593736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1713 0.3108018615453003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1010 0.18325153541199846 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 696 0.1262802659868821 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 587 0.10650361513548821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.721557456613838E-4 14-15 0.0 0.0 0.0 0.0 3.6287432754851176E-4 16-17 0.0 0.0 0.0 0.0 3.6287432754851176E-4 18-19 0.0 0.0 0.0 0.0 3.6287432754851176E-4 20-21 0.0 0.0 0.0 0.0 3.6287432754851176E-4 22-23 0.0 0.0 0.0 0.0 3.6287432754851176E-4 24-25 0.0 0.0 0.0 9.071858188712794E-5 3.6287432754851176E-4 26-27 0.0 0.0 0.0 0.0012700601464197912 3.6287432754851176E-4 28-29 0.0 0.0 0.0 0.0026308388747267103 3.6287432754851176E-4 30-31 0.0 0.0 0.0 0.005624552077001932 3.6287432754851176E-4 32-33 0.0 0.0 0.0 0.008890421024938538 3.6287432754851176E-4 34-35 0.0 0.0 0.0 0.012700601464197912 3.6287432754851176E-4 36-37 0.0 0.0 0.0 0.019141620778183994 3.6287432754851176E-4 38-39 0.0 0.0 0.0 0.027669167475574023 3.6287432754851176E-4 40-41 0.0 0.0 0.0 0.039281145957126395 3.6287432754851176E-4 42-43 0.0 0.0 0.0 0.053796119059066866 3.6287432754851176E-4 44-45 0.0 0.0 0.0 0.06622456477760338 3.6287432754851176E-4 46-47 0.0 0.0 0.0 0.09380301367129029 3.6287432754851176E-4 48-49 0.0 0.0 0.0 0.1272781703876405 3.6287432754851176E-4 50-51 0.0 0.0 0.0 0.14006949043372555 3.6287432754851176E-4 52-53 0.0 0.0 0.0 0.16029973419455507 3.6287432754851176E-4 54-55 0.0 0.0 0.0 0.19504495105732506 3.6287432754851176E-4 56-57 0.0 0.0 0.0 0.25764077255944334 3.6287432754851176E-4 58-59 0.0 0.0 0.0 0.3295806079959358 3.6287432754851176E-4 60-61 0.0 0.0 0.0 0.4086872114015113 3.6287432754851176E-4 62-63 0.0 0.0 0.0 0.4955956128493799 3.6287432754851176E-4 64-65 9.071858188712794E-5 0.0 0.0 0.5796917382587475 3.6287432754851176E-4 66-67 1.8143716377425588E-4 0.0 0.0 0.706153441409404 3.6287432754851176E-4 68-69 1.8143716377425588E-4 0.0 0.0 0.8557483829412779 3.6287432754851176E-4 70-71 1.8143716377425588E-4 0.0 0.0 0.9461948090827444 3.6287432754851176E-4 72-73 1.8143716377425588E-4 0.0 0.0 1.0633124982990267 3.6287432754851176E-4 74-75 1.8143716377425588E-4 0.0 0.0 1.1844218051183424 3.6287432754851176E-4 76-77 1.8143716377425588E-4 0.0 0.0 1.3244912955520678 3.6287432754851176E-4 78-79 1.8143716377425588E-4 0.0 0.0 1.473541925592619 4.535929094356397E-4 80-81 1.8143716377425588E-4 0.0 0.0 1.705327902314231 5.443114913227676E-4 82-83 1.8143716377425588E-4 0.0 0.0 1.9623336448004642 5.443114913227676E-4 84-85 1.8143716377425588E-4 0.0 0.0 2.367573550090265 5.443114913227676E-4 86-87 1.8143716377425588E-4 0.0 0.0 2.9181446235632444 5.443114913227676E-4 88-89 1.8143716377425588E-4 0.0 0.0 3.479511208280792 5.443114913227676E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 550 0.0 29.366385 2 GTACATG 2580 0.0 28.542084 1 ACATGGG 2585 0.0 27.749214 3 GTTACAC 140 5.792135E-7 27.1454 3 GTACCTG 615 0.0 27.037506 1 TACATGG 2680 0.0 26.94282 2 GTATCAA 3755 0.0 26.189915 1 GTGTAGC 260 0.0 25.58164 1 GGTATCA 2720 0.0 25.151697 1 GCCGCGC 95 0.0012244274 25.004608 8 GAGTACT 2045 0.0 24.277092 12-13 CATGGGG 1100 0.0 23.752222 4 TCATACC 125 2.2524052E-4 22.802135 4 ATGGGGA 605 0.0 22.774858 5 TCAACGC 4320 0.0 22.54262 4 TAGTCCA 170 3.1390246E-6 22.357063 9 TATCAAC 4420 0.0 22.355034 2 ATCAACG 4365 0.0 22.310223 3 CAACGCA 4350 0.0 22.281992 5 CATGGGA 1475 0.0 22.222418 4 >>END_MODULE