FastQCFastQC Report
Thu 26 May 2016
SRR936526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551155
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.5129228619898214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.4799012981829068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.2859449701082273No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12090.21935753100307537No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.13825511879598298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG4800.033.647792
GTACCTG5850.029.5950571
CTGGTCG1903.947207E-1027.4941169
GAACCGT700.00839660527.1370536
GTATCAA46550.025.0016251
CGTCTCA950.001227274524.9946528
GGTATCA34550.024.0807841
ATACGTC1000.001654616623.7449195
GAGTACT29500.023.01840812-13
CCCTTAC1050.002042296822.9009381
GGTAAGG2103.4391633E-822.6183153
GTACATG29350.021.6290951
GTACTTT31250.021.42547214-15
AGTACTT30800.021.1218112-13
TACATGG30050.020.8664422
ACATGGG27800.020.8446463
ATCAACG55500.020.711043
TCAACGC55550.020.6923964
TATCAAC55700.020.6385462
CAACGCA55750.020.6144245
Produced by FastQC (version 0.11.5)