FastQCFastQC Report
Thu 26 May 2016
SRR936525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602457
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.3562079949274388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.3150432313011551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.172460441160116No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7800.1294698210826665No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6440.10689559586825284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG550.002569380434.570845
TACGACG600.003933692431.692574
GGTATCA27300.028.9016081
GTATTGG1852.8740033E-1028.261651
TACCTGG4450.027.7732032
GTATCAA37900.026.4618241
GAGTACT21500.023.10609412-13
GTACATG31300.022.930271
ATCAACG44350.022.2937093
TCAACGC44450.022.2454034
CAACGCA44700.022.1191485
TATCAAC45250.021.85032
TACATGG32050.021.6539672
AACGCAG45900.021.5408736
GTACTTT22800.021.37163514-15
GGGCGCG1150.0037036620.6690677
GTGTAGC3451.8189894E-1220.6656321
ACATGGG31700.020.5434763
GTACCTG6250.020.5333731
AGTACTT22850.020.28463212-13
Produced by FastQC (version 0.11.5)