##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936525_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602457 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.547136144156347 31.0 31.0 34.0 30.0 34.0 2 31.629478950364923 33.0 31.0 34.0 30.0 34.0 3 31.664847449693504 33.0 31.0 34.0 30.0 34.0 4 35.29305328015111 37.0 35.0 37.0 33.0 37.0 5 35.26159709323653 37.0 35.0 37.0 33.0 37.0 6 35.36822213037611 37.0 35.0 37.0 33.0 37.0 7 35.24630637539277 37.0 35.0 37.0 32.0 37.0 8 35.20120440130997 37.0 35.0 37.0 32.0 37.0 9 36.72441352660854 39.0 37.0 39.0 32.0 39.0 10-11 36.623355691775515 39.0 37.0 39.0 32.0 39.0 12-13 36.540208678793675 39.0 37.0 39.0 32.0 39.0 14-15 37.63789365880055 40.0 37.0 41.0 32.0 41.0 16-17 37.503499004908235 40.0 37.0 41.0 32.0 41.0 18-19 37.46081546068848 40.0 37.0 41.0 32.0 41.0 20-21 37.38813226504132 40.0 37.0 41.0 32.0 41.0 22-23 37.23196095322986 39.0 37.0 41.0 31.5 41.0 24-25 37.363147909311365 40.0 37.0 41.0 32.0 41.0 26-27 37.3360248117293 40.0 37.0 41.0 31.5 41.0 28-29 37.24131431786833 39.5 37.0 41.0 31.0 41.0 30-31 37.053285960657774 39.0 36.0 41.0 31.0 41.0 32-33 36.87752735879906 39.0 36.0 41.0 30.5 41.0 34-35 36.6609251780625 39.0 35.5 41.0 30.0 41.0 36-37 36.48372082986835 39.0 35.0 40.5 30.0 41.0 38-39 36.27917179151375 39.0 35.0 40.0 30.0 41.0 40-41 36.091067080306146 39.0 35.0 40.0 30.0 41.0 42-43 35.86241839666565 38.0 35.0 40.0 29.0 41.0 44-45 35.59583007583944 38.0 35.0 40.0 27.5 41.0 46-47 35.320573916478686 38.0 35.0 40.0 27.0 41.0 48-49 35.229766605749454 38.5 35.0 40.0 26.0 41.0 50-51 34.33370348423207 37.5 33.5 39.5 25.0 40.5 52-53 34.49985310154916 38.0 34.0 39.5 25.5 40.5 54-55 34.91176133732366 38.0 34.0 40.0 26.0 41.0 56-57 34.825749389582995 38.0 34.0 40.0 25.5 41.0 58-59 34.58259925604649 38.0 34.0 40.0 25.0 41.0 60-61 34.26270090645473 38.0 34.0 40.0 23.5 41.0 62-63 33.92588682677768 37.0 33.5 40.0 23.0 41.0 64-65 33.584699820900084 37.0 33.0 40.0 22.0 41.0 66-67 33.20777001512141 36.0 32.5 39.0 22.0 41.0 68-69 32.78687109619442 36.0 32.0 39.0 20.5 40.5 70-71 32.34466277261282 35.0 32.0 38.5 19.5 40.0 72-73 31.945242565029538 35.0 31.0 38.0 18.0 40.0 74-75 31.504876696594117 35.0 31.0 37.0 17.0 39.0 76-77 31.050204412929055 35.0 31.0 36.5 12.0 39.0 78-79 30.591957600293465 34.5 30.0 36.0 9.0 39.0 80-81 30.123056915265323 34.0 30.0 36.0 7.0 37.5 82-83 29.65349975184951 34.0 29.0 35.0 4.0 37.0 84-85 29.29607258277354 34.0 29.0 35.0 2.0 36.5 86-87 28.965690497413092 34.0 29.0 35.0 2.0 36.0 88-89 28.54310017146452 34.0 29.0 35.0 2.0 36.0 90-91 28.122743199929623 34.0 27.5 35.0 2.0 35.0 92-93 27.758397694773237 34.0 27.0 35.0 2.0 35.0 94-95 27.301098667622753 33.0 26.0 35.0 2.0 35.0 96-97 26.712335486184077 33.0 25.0 35.0 2.0 35.0 98-99 25.972431227456898 33.0 24.5 35.0 2.0 35.0 100-101 23.505318221881396 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2563760611541035 1101 2 0.4736591982737153 1101 3 0.3442117475930466 1101 4 0.28442051900140797 1101 5 0.27828006137202976 1101 6 0.28824991670578015 1101 7 0.2973675583287445 1101 8 0.36513142520591657 1101 9 0.5900869036019571 1101 10-11 0.6185418295287164 1101 12-13 0.7057700591101792 1101 14-15 0.7589243696605763 1101 16-17 0.7049091343161109 1101 18-19 0.7457974500445346 1101 20-21 0.8069142181515332 1101 22-23 0.6183272450296187 1101 24-25 0.736189725676013 1101 26-27 0.5654630283928483 1101 28-29 0.676272894984038 1101 30-31 0.4024517338756013 1101 32-33 0.42234061833498515 1101 34-35 0.3872432151560261 1101 36-37 0.38973517312903283 1101 38-39 0.5242968454155346 1101 40-41 0.256380506514553 1101 42-43 0.2646904717784011 1101 44-45 0.3024688956570003 1101 46-47 0.5668684846741172 1101 48-49 1.0183497108109165 1101 50-51 0.8589206659150861 1101 52-53 0.7824596680091602 1101 54-55 0.8585421592226794 1101 56-57 0.6241639337703049 1101 58-59 0.5568337369810195 1101 60-61 0.6044734265882497 1101 62-63 0.7749744905132161 1101 64-65 0.8694108932609694 1101 66-67 0.643227855216999 1101 68-69 0.5531487757614926 1101 70-71 0.5643506987265212 1101 72-73 0.5447743059754622 1101 74-75 0.597492064527362 1101 76-77 0.7635163747758007 1101 78-79 0.5432178381058534 1101 80-81 0.5437662148433446 1101 82-83 0.7484201229020329 1101 84-85 0.7396457867849904 1101 86-87 0.48479158141936907 1101 88-89 0.5045525749885513 1101 90-91 0.5026635938965995 1101 92-93 0.7106042362514451 1101 94-95 0.7781455790642156 1101 96-97 0.22729556403505669 1101 98-99 0.6265327716483569 1101 100-101 1.1330220577785077 1102 1 0.3382282777561407 1102 2 0.45084903874687754 1102 3 0.4426710262688722 1102 4 0.3688492922886226 1102 5 0.5175293599863906 1102 6 0.44833618667455966 1102 7 0.42014309072094136 1102 8 0.1941506395422934 1102 9 0.663285129054394 1102 10-11 0.3669649917307751 1102 12-13 0.46017524498900997 1102 14-15 0.5392263704015221 1102 16-17 0.4947564025889051 1102 18-19 0.6708990508979298 1102 20-21 0.5697160187625272 1102 22-23 0.5123095702975604 1102 24-25 0.37159997344618745 1102 26-27 0.16661940713922974 1102 28-29 0.34980696640901243 1102 30-31 0.14205521595047088 1102 32-33 0.22533223521737966 1102 34-35 0.014169814938959746 1102 36-37 0.3144304636525561 1102 38-39 0.2806153666507214 1102 40-41 -0.06647703261318583 1102 42-43 0.2511933359637126 1102 44-45 0.22422367224682205 1102 46-47 0.15870299675731303 1102 48-49 0.41716726862986064 1102 50-51 0.5598246016798853 1102 52-53 0.6036840537033115 1102 54-55 0.713494025643854 1102 56-57 0.6059944618713402 1102 58-59 0.8776918383342718 1102 60-61 0.3267279695380054 1102 62-63 0.41260013023090636 1102 64-65 0.6096873704526686 1102 66-67 0.758568327449197 1102 68-69 0.4805872427847291 1102 70-71 0.4558583939556087 1102 72-73 0.6480869685228647 1102 74-75 0.46392533934207947 1102 76-77 0.5455790536128511 1102 78-79 0.5272651544098359 1102 80-81 0.5407963795456325 1102 82-83 0.7552948192164806 1102 84-85 0.7513743323894886 1102 86-87 0.5964920157478915 1102 88-89 0.46174617604300394 1102 90-91 0.21420606519424013 1102 92-93 0.29487150932735773 1102 94-95 0.3219088783971813 1102 96-97 0.43374216843493585 1102 98-99 0.5796483760128623 1102 100-101 0.7401739573374719 1103 1 0.40306236994293343 1103 2 0.5238688636648661 1103 3 0.4763437328736799 1103 4 0.3426724516313726 1103 5 0.5101467557495738 1103 6 0.35405713864953725 1103 7 0.3957228362321672 1103 8 0.19623362536810873 1103 9 0.5893859814002482 1103 10-11 0.535973601680098 1103 12-13 0.6146848114849632 1103 14-15 0.418317949702228 1103 16-17 0.5175662814812441 1103 18-19 0.3934016816585739 1103 20-21 0.4975497752827991 1103 22-23 0.5508767272844608 1103 24-25 0.4181330324802488 1103 26-27 0.419373726014193 1103 28-29 0.07965667841537538 1103 30-31 0.32771896046499904 1103 32-33 0.35825411964513876 1103 34-35 0.2754532554590625 1103 36-37 0.220953514469997 1103 38-39 0.41345333444732546 1103 40-41 0.34283946385202313 1103 42-43 0.41996409632046294 1103 44-45 0.1859293730176219 1103 46-47 0.23924236961692458 1103 48-49 0.47768094137764905 1103 50-51 0.8049924452386961 1103 52-53 0.6383498595809769 1103 54-55 0.8024755145617135 1103 56-57 0.7522982174237356 1103 58-59 0.5050551468941791 1103 60-61 0.5079132430444346 1103 62-63 0.6593793790380573 1103 64-65 0.8532947765329695 1103 66-67 0.7333748043716142 1103 68-69 0.7817635171334203 1103 70-71 0.5734168421466137 1103 72-73 0.7347507109780942 1103 74-75 0.5142331022997375 1103 76-77 0.4259041559087713 1103 78-79 0.5686741506845543 1103 80-81 0.41052070054168155 1103 82-83 0.40879275270508586 1103 84-85 0.24309232720415963 1103 86-87 0.24413483361688293 1103 88-89 0.5032169514326732 1103 90-91 0.6262429020211826 1103 92-93 0.6970890706585742 1103 94-95 0.6810597038691242 1103 96-97 0.38591792589836516 1103 98-99 0.5574552586181305 1103 100-101 0.9809626912980711 1104 1 0.0642658291112852 1104 2 0.2477457066189217 1104 3 0.19613704090685147 1104 4 0.3645955598524466 1104 5 0.24550114296042125 1104 6 0.16459443419537223 1104 7 0.27691194384663476 1104 8 0.38562605159446406 1104 9 0.4334607870254672 1104 10-11 0.4541974859207656 1104 12-13 0.28536103599061136 1104 14-15 0.26414416274143093 1104 16-17 0.1439006430960177 1104 18-19 0.3550926497966458 1104 20-21 0.48563946509689515 1104 22-23 0.18816524530158318 1104 24-25 0.2725683284255638 1104 26-27 -0.23076830998305553 1104 28-29 -0.057243459921764384 1104 30-31 0.1040825430271255 1104 32-33 -0.2106370378742639 1104 34-35 -0.40196598006177453 1104 36-37 -0.20367493563615824 1104 38-39 -0.14903695606067657 1104 40-41 -0.11372489251037621 1104 42-43 -0.14252249533344497 1104 44-45 0.056885600778265655 1104 46-47 0.07513900883809299 1104 48-49 0.05807553513250241 1104 50-51 0.20406736182953722 1104 52-53 0.3886790575954322 1104 54-55 0.4952671877013657 1104 56-57 0.2940379364587855 1104 58-59 0.36669026233541757 1104 60-61 0.44362900830036267 1104 62-63 0.44068648009815803 1104 64-65 0.08667767451117214 1104 66-67 0.23849771729319258 1104 68-69 0.26603229066738976 1104 70-71 0.35414507254083816 1104 72-73 0.19035336527579005 1104 74-75 0.17747093122033775 1104 76-77 0.2959837552488942 1104 78-79 0.2254112435331912 1104 80-81 0.12124441833390875 1104 82-83 0.31207696525603623 1104 84-85 0.44587275582737007 1104 86-87 0.4903379598883646 1104 88-89 0.36178571682915717 1104 90-91 0.47322130811097196 1104 92-93 0.19299258974836064 1104 94-95 0.3556678102777546 1104 96-97 0.022956771264073694 1104 98-99 0.13633702104812784 1104 100-101 0.8868730902564756 1105 1 0.209591032363317 1105 2 0.1696203205018385 1105 3 0.1404327015686242 1105 4 0.15946390937180865 1105 5 0.3540958003355641 1105 6 0.24632460939986345 1105 7 0.2438927553720731 1105 8 0.386946572018509 1105 9 0.5125406545718576 1105 10-11 0.4870792167137523 1105 12-13 0.3704952193993094 1105 14-15 0.029112377583146554 1105 16-17 0.17999795695669008 1105 18-19 0.328078354197757 1105 20-21 0.311340035932389 1105 22-23 0.273764313678285 1105 24-25 0.3212731372821622 1105 26-27 0.36814021424655863 1105 28-29 0.13347212314591417 1105 30-31 0.13263126791407132 1105 32-33 0.28029075514226776 1105 34-35 0.2531503801823689 1105 36-37 0.3105094615269621 1105 38-39 0.2625916549062808 1105 40-41 0.45975627733171365 1105 42-43 0.7875980349752041 1105 44-45 0.4089680074305946 1105 46-47 0.4853405310660861 1105 48-49 0.620013348659576 1105 50-51 0.6561000366015719 1105 52-53 0.8525006591473172 1105 54-55 1.0090569732056096 1105 56-57 1.0904368540953726 1105 58-59 0.9595614598543847 1105 60-61 0.7995756747199181 1105 62-63 0.8095719299708861 1105 64-65 1.1553470532642294 1105 66-67 1.2994957218401026 1105 68-69 1.1495979120378834 1105 70-71 1.2793997553688214 1105 72-73 1.155382741063466 1105 74-75 1.2515521270095 1105 76-77 0.905552199907504 1105 78-79 1.003180011912363 1105 80-81 1.0125825765863112 1105 82-83 1.10002377994455 1105 84-85 1.3547591632704723 1105 86-87 1.2887462308251756 1105 88-89 1.1392618084593735 1105 90-91 1.0041848092353476 1105 92-93 1.1624383727320229 1105 94-95 0.9290509063807519 1105 96-97 0.9051207876230798 1105 98-99 1.1426152848186106 1105 100-101 1.12744256661993 1106 1 0.07460639334642138 1106 2 -0.05996101100125273 1106 3 0.15848389939847607 1106 4 0.2665475286808885 1106 5 -0.024552433151377784 1106 6 -0.08039451338995462 1106 7 0.04522335967968161 1106 8 0.0836141374661068 1106 9 0.4275037228465308 1106 10-11 0.23369244676899115 1106 12-13 0.570045011862625 1106 14-15 0.4371987504301629 1106 16-17 0.4450321106318924 1106 18-19 0.428506909284863 1106 20-21 0.2515343360271771 1106 22-23 0.39413354922425015 1106 24-25 0.28886595683964345 1106 26-27 -0.1307720565643038 1106 28-29 0.4336710805600461 1106 30-31 0.43704121823599706 1106 32-33 0.14965294751407043 1106 34-35 -0.15915004959832402 1106 36-37 -0.13531856082817484 1106 38-39 4.7036850565262966E-4 1106 40-41 -0.045274853171271445 1106 42-43 0.14871799771665195 1106 44-45 0.3363618287202641 1106 46-47 0.34230771739153454 1106 48-49 0.21605013331166134 1106 50-51 0.302377653688211 1106 52-53 0.24025824159004117 1106 54-55 0.5835179089182603 1106 56-57 0.37091778359826577 1106 58-59 0.29117791735021825 1106 60-61 0.5199843340656045 1106 62-63 0.5817452639578775 1106 64-65 0.32200044248539683 1106 66-67 0.3853262362696199 1106 68-69 0.26134906411162007 1106 70-71 0.5136557690302936 1106 72-73 0.6038448043376441 1106 74-75 0.49742896951039484 1106 76-77 0.6390769327244463 1106 78-79 0.4884961785300064 1106 80-81 0.6890388059551995 1106 82-83 0.7925947427840399 1106 84-85 0.7453744605218375 1106 86-87 0.8775845973316976 1106 88-89 0.7710435190845963 1106 90-91 0.8053744683522552 1106 92-93 1.1828018887630058 1106 94-95 1.2950293928336727 1106 96-97 1.4814446510737476 1106 98-99 1.6633873376342443 1106 100-101 2.2360357293483055 1107 1 -0.12367964432841205 1107 2 -0.10036848128987685 1107 3 -0.15355426051744203 1107 4 0.03320611061960932 1107 5 0.10672737928292975 1107 6 0.0992481416347033 1107 7 0.04025933671905335 1107 8 0.11058478990364762 1107 9 0.1318775606668865 1107 10-11 0.13062025014840373 1107 12-13 0.030033693243503023 1107 14-15 -0.3029015001964055 1107 16-17 0.19949083809570567 1107 18-19 0.351192655909486 1107 20-21 0.2262776459147915 1107 22-23 0.2901396936174905 1107 24-25 0.3338251289634968 1107 26-27 -0.022962209365665842 1107 28-29 0.09184878288036202 1107 30-31 0.170908143613957 1107 32-33 0.0796068645647594 1107 34-35 0.2708685452759596 1107 36-37 0.2890568939377687 1107 38-39 0.26505926008945124 1107 40-41 -0.006371142281146547 1107 42-43 0.33144901881778566 1107 44-45 0.48662458237860307 1107 46-47 0.42761776670838003 1107 48-49 0.3594570376693369 1107 50-51 0.21835307611524968 1107 52-53 0.1539214318236901 1107 54-55 0.3769531098237522 1107 56-57 0.3346577787474345 1107 58-59 0.525715875147938 1107 60-61 0.35204028038301516 1107 62-63 0.2149330721881313 1107 64-65 0.46494651734997916 1107 66-67 0.34122404530834416 1107 68-69 0.48155279925592964 1107 70-71 0.625451387107745 1107 72-73 0.6402341648356646 1107 74-75 0.5602877293131279 1107 76-77 0.3448009201122737 1107 78-79 0.3970659901558449 1107 80-81 0.7347660272342083 1107 82-83 1.0188377697061846 1107 84-85 0.5620290000965795 1107 86-87 0.5968909276736802 1107 88-89 0.4974724947495659 1107 90-91 0.39730590985924863 1107 92-93 0.6616764946325198 1107 94-95 0.43224339558248204 1107 96-97 0.5343511107713788 1107 98-99 1.2797993353705195 1107 100-101 1.0654964779941132 1108 1 0.0907638718694912 1108 2 0.20322383282875478 1108 3 0.18323214995317727 1108 4 0.3724245270310078 1108 5 0.17155611779588043 1108 6 0.18102666200971385 1108 7 0.2529948792791572 1108 8 0.31886474906080053 1108 9 0.41788888527469936 1108 10-11 0.0577807439755631 1108 12-13 0.2731228977362079 1108 14-15 0.0659465458446249 1108 16-17 0.2593348926766126 1108 18-19 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74-75 -0.8711900416313831 2316 76-77 -0.5939337794334243 2316 78-79 -1.5064355076519966 2316 80-81 -1.4921032567938042 2316 82-83 -1.0151396540643205 2316 84-85 -1.0419367189503745 2316 86-87 -1.5909659176057431 2316 88-89 -1.7531692811942499 2316 90-91 -1.4444482115921389 2316 92-93 -2.5721620595365415 2316 94-95 -2.8297856876797667 2316 96-97 -3.707533944875653 2316 98-99 -5.301859957981591 2316 100-101 -5.761676335305626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1402.0 3 528.0 4 527.0 5 549.0 6 624.0 7 730.0 8 864.0 9 1103.0 10 1306.0 11 1693.0 12 2210.0 13 2766.0 14 2971.0 15 3768.0 16 4581.0 17 5919.0 18 5070.0 19 3614.0 20 3568.0 21 3964.0 22 4413.0 23 4934.0 24 5699.0 25 6507.0 26 7538.0 27 8697.0 28 10746.0 29 12815.0 30 15808.0 31 19408.0 32 24481.0 33 31741.0 34 43167.0 35 59839.0 36 84680.0 37 112105.0 38 88683.0 39 13436.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45846589135445 18.501059357212597 14.48795520811886 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44-45 21.94105153778417 26.435761812557736 29.198021716631406 22.425164933026693 46-47 22.146236769747755 26.71629143777663 28.743664459307716 22.3938073331679 48-49 22.200330151605815 26.716219838808335 28.556205032612635 22.52724497697321 50-51 22.0847750075317 26.852943837159216 28.665365874710663 22.39691528059842 52-53 22.326134192516015 26.842404051570877 28.417765959036107 22.413695796876997 54-55 21.872178029800004 26.900513930572895 28.474592308305226 22.752715731321878 56-57 22.149877690335337 27.222553785955185 28.136867295466452 22.490701228243033 58-59 22.263105428594432 26.762572969329852 28.307742364417944 22.66657923765778 60-61 22.147953492078248 26.811777048831992 28.731872269220045 22.30839718986972 62-63 22.022336316267367 26.882261253230684 28.744644579343227 22.35075785115873 64-65 21.935839404306034 27.01736389485059 28.471907538629317 22.574889162214067 66-67 21.879040999702866 27.146969136099624 28.14552661851635 22.828463245681164 68-69 21.86797022988334 26.893794129317055 28.51818464046591 22.720051000333694 70-71 21.992755788397307 26.93522041393598 28.224703359162834 22.847320438503882 72-73 21.900523616698305 26.92679574633016 28.31364548933553 22.859035147636007 74-75 21.871690508835822 27.30780467237905 28.06755594803746 22.752948870747666 76-77 21.901928072840153 27.077347011611597 28.201406007486536 22.819318908061717 78-79 21.932133952611295 27.01636062427999 28.14555245326366 22.90595296984506 80-81 22.004575207884763 27.26332512695538 27.884696792872237 22.84740287228762 82-83 22.1740086734588 27.082771356836055 28.020500902620714 22.722719067084434 84-85 21.842290062902688 27.055460281991305 27.845105659581744 23.257143995524263 86-87 22.042451193742707 26.988727377152316 28.08362091718083 22.885200511924147 88-89 22.011583165401227 26.871350174129272 27.917121770972702 23.199944889496802 90-91 21.634344846131757 26.90590291668257 28.35262461593112 23.10712762125455 92-93 21.818352361489026 26.830778629806773 28.368000159374983 22.982868849329215 94-95 21.42981053113865 26.891513783741384 28.382729257986256 23.295946427133714 96-97 21.49809096945551 26.847360557768923 28.44546812749004 23.209080345285525 98-99 21.380157639802597 27.018582237920715 28.06984630326777 23.53141381900892 100-101 21.35419174752042 26.980218159838863 28.17222638276109 23.493363709879628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 50.0 1 41.0 2 72.0 3 114.5 4 101.5 5 96.5 6 155.5 7 303.0 8 483.0 9 632.0 10 612.5 11 504.5 12 535.5 13 633.0 14 706.5 15 768.0 16 814.0 17 835.5 18 877.0 19 961.0 20 1077.5 21 1208.5 22 1392.5 23 1652.5 24 1923.5 25 2365.0 26 2981.0 27 3728.5 28 4475.5 29 5351.5 30 6558.5 31 7937.5 32 9668.0 33 11147.5 34 12395.0 35 13787.0 36 15631.5 37 17290.0 38 18144.0 39 18435.0 40 18556.5 41 19066.0 42 19328.0 43 19956.5 44 20864.0 45 21879.0 46 22468.5 47 22944.0 48 25215.5 49 25382.5 50 22881.0 51 21298.0 52 20390.0 53 19805.0 54 19188.5 55 17792.0 56 15877.5 57 14228.0 58 13235.5 59 11670.0 60 9524.0 61 8047.5 62 6728.5 63 5190.5 64 3721.0 65 2655.0 66 1894.0 67 1482.0 68 1196.5 69 907.0 70 691.5 71 503.0 72 378.0 73 307.5 74 226.5 75 165.0 76 127.0 77 82.0 78 48.5 79 32.5 80 26.5 81 17.5 82 12.0 83 11.5 84 8.5 85 5.0 86 5.5 87 4.5 88 2.0 89 3.0 90 2.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03402732477172645 2 0.023902120815261503 3 0.019752447062611936 4 0.050460032832218726 5 0.057099510836458034 6 0.04996207198190079 7 0.053945758784444374 8 0.04531443737893327 9 0.04315660702755549 10-11 0.05145595453285463 12-13 0.042658646177237546 14-15 0.03626814859815721 16-17 0.04489947000366831 18-19 0.04697430687999309 20-21 0.010374184381623916 22-23 0.018258564511658092 24-25 0.005643556303603411 26-27 0.009710236581199986 28-29 0.006390497579080333 30-31 0.008465334455405116 32-33 0.0034857259522256363 34-35 0.008299347505299134 36-37 0.009212275730882038 38-39 0.011536093032365795 40-41 0.0019088499262188007 42-43 0.0023238173014837574 44-45 0.0025727977266427316 46-47 4.1496737526495667E-4 48-49 0.0010789151756888872 50-51 5.809543253709393E-4 52-53 0.003983686802543584 54-55 0.006805464954345289 56-57 0.015353792884803397 58-59 0.0105401713317299 60-61 0.010789151756888873 62-63 0.00497960850317948 64-65 0.0 66-67 0.006307504104027341 68-69 0.01792659061144613 70-71 0.00746941275476922 72-73 0.0020748368763247836 74-75 0.0038176998524376013 76-77 0.006971451904451273 78-79 0.005809543253709393 80-81 0.013278956008478613 82-83 0.007386419279716229 84-85 0.016266721110386302 86-87 0.003983686802543584 88-89 0.005145595453285463 90-91 0.004315660702755549 92-93 0.017262642811022195 94-95 0.010374184381623916 96-97 0.009461256156041011 98-99 0.02232524478925467 100-101 0.03950489412522387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 602457.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.58028778148436 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09033906025077 61.46861193859549 2 7.9102909699256525 11.166212261852593 3 2.0178765499741256 4.272669228140479 4 0.869177428400099 2.4538717211859806 5 0.49334342911285783 1.741016060094492 6 0.32463739386900003 1.3747800410343065 7 0.22457281165893006 1.1095289572349059 8 0.14913640511558182 0.8420872313403043 9 0.1365682199656945 0.8675121840285398 >10 0.7182405154981064 9.234256518747705 >50 0.045837021219872126 2.2049054909951975 >100 0.018569828302807803 2.101043884570907 >500 7.051833532579098E-4 0.3202001498083165 >1k 7.051833532579098E-4 0.843304332370815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.3562079949274388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1898 0.3150432313011551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.172460441160116 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 780 0.1294698210826665 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 644 0.10689559586825284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.6598695010598268E-4 0.0 5 0.0 0.0 0.0 1.6598695010598268E-4 0.0 6 0.0 0.0 0.0 1.6598695010598268E-4 0.0 7 0.0 0.0 0.0 1.6598695010598268E-4 0.0 8 0.0 0.0 0.0 1.6598695010598268E-4 0.0 9 0.0 0.0 0.0 1.6598695010598268E-4 0.0 10-11 0.0 0.0 0.0 1.6598695010598268E-4 0.0 12-13 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 14-15 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 16-17 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 18-19 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 20-21 0.0 0.0 0.0 2.48980425158974E-4 2.48980425158974E-4 22-23 0.0 0.0 0.0 4.97960850317948E-4 3.3197390021196536E-4 24-25 0.0 0.0 0.0 9.95921700635896E-4 3.3197390021196536E-4 26-27 0.0 0.0 0.0 0.0020748368763247836 3.3197390021196536E-4 28-29 1.6598695010598268E-4 0.0 0.0 0.0033197390021196533 3.3197390021196536E-4 30-31 1.6598695010598268E-4 0.0 0.0 0.005892536728762384 3.3197390021196536E-4 32-33 1.6598695010598268E-4 0.0 0.0 0.009544249631094005 3.3197390021196536E-4 34-35 1.6598695010598268E-4 0.0 0.0 0.01311296905837263 3.3197390021196536E-4 36-37 1.6598695010598268E-4 0.0 0.0 0.01867353188692305 3.3197390021196536E-4 38-39 1.6598695010598268E-4 0.0 0.0 0.027221859817381157 3.3197390021196536E-4 40-41 1.6598695010598268E-4 0.0 0.0 0.03651712902331619 3.3197390021196536E-4 42-43 1.6598695010598268E-4 0.0 0.0 0.04996207198190078 3.3197390021196536E-4 44-45 1.6598695010598268E-4 0.0 0.0 0.0641539562159623 3.3197390021196536E-4 46-47 1.6598695010598268E-4 0.0 0.0 0.09311867900945628 3.3197390021196536E-4 48-49 1.6598695010598268E-4 0.0 0.0 0.12631606903065282 4.97960850317948E-4 50-51 1.6598695010598268E-4 0.0 0.0 0.13918005766386649 4.97960850317948E-4 52-53 1.6598695010598268E-4 0.0 0.0 0.15694066132520662 4.97960850317948E-4 54-55 1.6598695010598268E-4 0.0 0.0 0.19271084907304586 4.97960850317948E-4 56-57 1.6598695010598268E-4 0.0 0.0 0.2499763468596099 4.97960850317948E-4 58-59 1.6598695010598268E-4 0.0 0.0 0.32342557228150726 4.97960850317948E-4 60-61 2.48980425158974E-4 0.0 0.0 0.4073319755600815 4.97960850317948E-4 62-63 3.3197390021196536E-4 0.0 0.0 0.4958030199665702 4.97960850317948E-4 64-65 3.3197390021196536E-4 0.0 0.0 0.5827801818221052 4.97960850317948E-4 66-67 3.3197390021196536E-4 0.0 0.0 0.7040336488745255 4.97960850317948E-4 68-69 3.3197390021196536E-4 0.0 0.0 0.8584015124730893 4.97960850317948E-4 70-71 3.3197390021196536E-4 0.0 0.0 0.938075248523961 4.97960850317948E-4 72-73 3.3197390021196536E-4 0.0 0.0 1.052440257146983 5.809543253709393E-4 74-75 3.3197390021196536E-4 0.0 0.0 1.1706229656224427 6.639478004239307E-4 76-77 3.3197390021196536E-4 0.0 0.0 1.2983499237289964 6.639478004239307E-4 78-79 3.3197390021196536E-4 0.0 0.0 1.4326333663647364 7.46941275476922E-4 80-81 3.3197390021196536E-4 0.0 0.0 1.6538939708560112 8.299347505299133E-4 82-83 3.3197390021196536E-4 0.0 0.0 1.9039533111906741 9.95921700635896E-4 84-85 3.3197390021196536E-4 0.0 0.0 2.2856403029593815 9.95921700635896E-4 86-87 3.3197390021196536E-4 0.0 0.0 2.804183535090471 9.95921700635896E-4 88-89 3.3197390021196536E-4 0.0 0.0 3.35127652263979 9.95921700635896E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGACGG 55 0.0025693804 34.57084 5 TACGACG 60 0.0039336924 31.69257 4 GGTATCA 2730 0.0 28.901608 1 GTATTGG 185 2.8740033E-10 28.26165 1 TACCTGG 445 0.0 27.773203 2 GTATCAA 3790 0.0 26.461824 1 GAGTACT 2150 0.0 23.106094 12-13 GTACATG 3130 0.0 22.93027 1 ATCAACG 4435 0.0 22.293709 3 TCAACGC 4445 0.0 22.245403 4 CAACGCA 4470 0.0 22.119148 5 TATCAAC 4525 0.0 21.8503 2 TACATGG 3205 0.0 21.653967 2 AACGCAG 4590 0.0 21.540873 6 GTACTTT 2280 0.0 21.371635 14-15 GGGCGCG 115 0.00370366 20.669067 7 GTGTAGC 345 1.8189894E-12 20.665632 1 ACATGGG 3170 0.0 20.543476 3 GTACCTG 625 0.0 20.533373 1 AGTACTT 2285 0.0 20.284632 12-13 >>END_MODULE