FastQCFastQC Report
Thu 26 May 2016
SRR936525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602457
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.5321541620397804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.4544722693901805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.28184584127995854No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12910.21428915258682363No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.1359433121367998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5450.030.5030442
TATCACG700.0083905327.1414832
AGTGCGA350.00839550227.14035668-69
GTACCTG6050.026.6950781
GGTATCA36900.025.8748191
GTAAGGT2059.567884E-1025.4843964
TAAGGTG2451.0913936E-1125.200715
GTATCAA50550.025.18381
GTACATG32850.023.7146191
GAGTACT32300.022.87028512-13
ATCACGC1050.002195663522.61793
AGTACTT33100.022.53281212-13
ACATGGG32050.022.526163
TACATGG34050.022.3189872
GTACTTT33250.022.14357214-15
GTATAGT1553.9014783E-521.4523071
ATCAACG59950.020.9956023
TCAACGC60000.020.976364
TATCAAC60050.020.9606362
CAACGCA60800.020.7784735
Produced by FastQC (version 0.11.5)