##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936525_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602457 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06726123192195 31.0 31.0 34.0 31.0 34.0 2 32.21222759466651 33.0 31.0 34.0 30.0 34.0 3 32.31195089442068 34.0 31.0 34.0 31.0 34.0 4 36.02473039569629 37.0 35.0 37.0 35.0 37.0 5 35.83093067223055 37.0 35.0 37.0 35.0 37.0 6 35.94772738967262 37.0 35.0 37.0 35.0 37.0 7 35.89551951425579 37.0 35.0 37.0 35.0 37.0 8 35.870868792295546 37.0 35.0 37.0 35.0 37.0 9 37.54912300794911 39.0 37.0 39.0 35.0 39.0 10-11 37.41859750986377 39.0 37.0 39.0 34.5 39.0 12-13 37.32521989121216 39.0 37.0 39.0 34.0 39.0 14-15 38.4700891515909 40.0 38.0 41.0 34.0 41.0 16-17 38.394229795653466 40.0 38.0 41.0 34.0 41.0 18-19 38.54814451487824 40.0 38.0 41.0 34.0 41.0 20-21 38.58056674584245 40.0 38.0 41.0 34.0 41.0 22-23 38.48635753257079 40.0 38.0 41.0 34.0 41.0 24-25 38.35544777469595 40.0 38.0 41.0 34.0 41.0 26-27 38.15046634033632 40.0 38.0 41.0 33.5 41.0 28-29 38.0778478795997 40.0 37.5 41.0 33.5 41.0 30-31 38.0521663786793 40.0 37.0 41.0 33.5 41.0 32-33 37.898894028951446 40.0 37.0 41.0 33.0 41.0 34-35 37.72222166893239 40.0 37.0 41.0 33.0 41.0 36-37 37.48702480011021 40.0 36.5 41.0 33.0 41.0 38-39 37.31039310689394 40.0 36.0 41.0 32.0 41.0 40-41 37.0730566662849 39.0 36.0 41.0 31.5 41.0 42-43 36.825802505407026 39.0 36.0 41.0 31.0 41.0 44-45 36.53532119304781 39.0 35.0 41.0 30.5 41.0 46-47 36.121509086955584 39.0 35.0 40.0 30.0 41.0 48-49 35.704481813639816 39.0 35.0 40.0 28.5 41.0 50-51 35.369342542289324 38.5 35.0 40.0 27.0 41.0 52-53 35.09259001057337 38.0 34.5 40.0 26.5 41.0 54-55 34.83556336800801 38.0 34.0 40.0 26.0 41.0 56-57 34.51941964322765 38.0 34.0 40.0 25.5 41.0 58-59 34.181335763382286 38.0 33.5 40.0 25.0 41.0 60-61 33.827600974011425 37.0 33.0 40.0 23.5 41.0 62-63 33.425813792519634 36.5 33.0 39.0 23.0 41.0 64-65 33.01878225333924 36.0 32.0 39.0 22.0 40.0 66-67 32.56405187424165 35.5 31.5 39.0 20.5 40.0 68-69 32.27265763365685 35.0 31.0 38.0 20.0 40.0 70-71 31.8326511269684 35.0 31.0 38.0 18.5 40.0 72-73 31.380455368598923 35.0 31.0 37.0 18.0 39.0 74-75 30.843639463065415 34.0 30.0 36.5 14.0 39.0 76-77 29.446006934934776 33.0 28.5 35.0 11.5 37.5 78-79 30.201897396826663 34.0 30.0 36.0 9.5 37.5 80-81 30.011096227614587 34.0 30.0 35.0 7.0 37.0 82-83 29.666621020255388 34.0 29.5 35.0 6.0 37.0 84-85 29.242665451642193 34.0 29.0 35.0 2.0 36.0 86-87 28.870354232750223 34.0 29.0 35.0 2.0 36.0 88-89 28.464819065925038 33.5 29.0 35.0 2.0 35.5 90-91 28.001891421296456 33.0 27.5 35.0 2.0 35.0 92-93 27.54221048141195 33.0 27.0 35.0 2.0 35.0 94-95 26.955610939867906 33.0 25.5 34.5 2.0 35.0 96-97 26.210553616274687 32.0 25.0 34.0 2.0 35.0 98-99 25.271817739689304 32.0 22.0 34.0 2.0 35.0 100-101 22.085630177755426 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28666829155158524 1101 2 0.2891468085466826 1101 3 0.24577001181553015 1101 4 0.15076368954973418 1101 5 0.11985426355529682 1101 6 0.19882861413157826 1101 7 0.17325773800811817 1101 8 0.10008442173684529 1101 9 0.2962717947202904 1101 10-11 0.27186593386381475 1101 12-13 0.197791833503139 1101 14-15 0.4259040203956772 1101 16-17 0.43634976242869783 1101 18-19 0.24013428467326037 1101 20-21 0.07967346780777973 1101 22-23 -0.03796144706416982 1101 24-25 0.03799212674054786 1101 26-27 0.0703762001418724 1101 28-29 0.12881571866306274 1101 30-31 0.14206623121668116 1101 32-33 0.13357248239104536 1101 34-35 0.02697181625115519 1101 36-37 -0.022943823352015613 1101 38-39 0.2747558007866502 1101 40-41 0.33680912110230565 1101 42-43 0.3547801997949236 1101 44-45 0.34129325777615804 1101 46-47 0.29503174650677266 1101 48-49 0.3500296166374852 1101 50-51 0.18448546044028546 1101 52-53 0.29430935348363363 1101 54-55 0.3025914009096553 1101 56-57 0.11639831121429012 1101 58-59 0.21549292064391068 1101 60-61 0.2703845305770969 1101 62-63 0.38326360099901535 1101 64-65 0.23775347019648052 1101 66-67 0.11093023862078155 1101 68-69 0.014355216984171193 1101 70-71 0.11087849161065222 1101 72-73 0.1956901940459339 1101 74-75 0.1574541574947439 1101 76-77 -0.11454149340062258 1101 78-79 -0.17194764906339444 1101 80-81 -0.05375817935734872 1101 82-83 0.01785926692538453 1101 84-85 -0.143904630134994 1101 86-87 -0.05415392364453098 1101 88-89 -0.060980580720450206 1101 90-91 -0.04755247947626273 1101 92-93 -0.16958196712186435 1101 94-95 -0.3116525201544995 1101 96-97 -0.29318162204600995 1101 98-99 -0.03826438575239166 1101 100-101 0.08315962196160953 1102 1 0.13653173777061767 1102 2 0.1998451038118887 1102 3 0.29175987313944063 1102 4 0.10953040505430778 1102 5 0.09348923770595263 1102 6 0.0244893687424792 1102 7 0.23753947629028005 1102 8 0.09160822044521666 1102 9 0.18738807301075155 1102 10-11 0.1710534993714674 1102 12-13 0.053991021440985776 1102 14-15 0.13008450223411216 1102 16-17 0.2148757262638199 1102 18-19 0.19442134734279648 1102 20-21 0.02715797402670006 1102 22-23 -0.032496503314654035 1102 24-25 0.10865987193696469 1102 26-27 -0.10256678981811973 1102 28-29 -0.22965052267317532 1102 30-31 0.07733443326787182 1102 32-33 0.1286395119600101 1102 34-35 0.17714793060261513 1102 36-37 0.13692183825896365 1102 38-39 0.04703538325043155 1102 40-41 0.20307577610248728 1102 42-43 0.285417488006253 1102 44-45 0.39505467681847506 1102 46-47 0.41047228371139255 1102 48-49 0.3367330761860998 1102 50-51 0.5147636317552369 1102 52-53 0.5842082832073103 1102 54-55 0.6061377966158901 1102 56-57 0.5903469774180223 1102 58-59 0.42100775725410244 1102 60-61 0.7460566413988161 1102 62-63 0.596535101936368 1102 64-65 0.5348217061301384 1102 66-67 0.6813885455028341 1102 68-69 0.5166460067248622 1102 70-71 0.4669697024619701 1102 72-73 0.365426916122221 1102 74-75 0.2073502760908177 1102 76-77 -0.062313162819464196 1102 78-79 0.09046599253831644 1102 80-81 0.6349224709625823 1102 82-83 0.5499349774797686 1102 84-85 0.377637264043738 1102 86-87 0.3396338567002921 1102 88-89 0.2354458431342934 1102 90-91 0.35143728317893164 1102 92-93 0.1868559804943537 1102 94-95 0.2950670056399254 1102 96-97 0.5634573863519954 1102 98-99 0.3303768343142188 1102 100-101 0.6355848245582685 1103 1 0.126556470009632 1103 2 0.20084283651836898 1103 3 0.13030530447093014 1103 4 0.2012219378147435 1103 5 0.09358190637838248 1103 6 0.21307526539425226 1103 7 0.10053502201608921 1103 8 0.05367481421252762 1103 9 0.07064819809286149 1103 10-11 0.10966153353943042 1103 12-13 0.0615378551583845 1103 14-15 0.10627534615741752 1103 16-17 0.3452502518517022 1103 18-19 0.11067049015757391 1103 20-21 0.2344845651992884 1103 22-23 0.2493320967088195 1103 24-25 0.18616280129666762 1103 26-27 0.23753290108037106 1103 28-29 0.12453377678254895 1103 30-31 0.18184152859922165 1103 32-33 -0.034502141372669826 1103 34-35 0.11471138290193039 1103 36-37 0.3090528676431674 1103 38-39 0.3649435012320623 1103 40-41 0.32185951007338076 1103 42-43 0.016110484931708413 1103 44-45 0.23945985720320095 1103 46-47 0.4388186009553863 1103 48-49 0.2672392942540185 1103 50-51 0.44883398992995893 1103 52-53 0.4335758710005777 1103 54-55 0.41995613718767544 1103 56-57 0.4828919886515237 1103 58-59 0.6949929838084117 1103 60-61 0.6718316089133012 1103 62-63 0.45818077400046775 1103 64-65 0.3424374235607104 1103 66-67 0.5889510299293477 1103 68-69 0.6681556823639099 1103 70-71 0.6458624559777242 1103 72-73 0.48278303771175857 1103 74-75 0.4938071614761448 1103 76-77 0.222289319465375 1103 78-79 0.32306744928984443 1103 80-81 0.4314256700910839 1103 82-83 0.6948044279133541 1103 84-85 0.720066542401895 1103 86-87 0.190684925376015 1103 88-89 0.44896579058086417 1103 90-91 0.32036341800979073 1103 92-93 0.30139394479014214 1103 94-95 0.3080910727238084 1103 96-97 0.19895134126560166 1103 98-99 0.637223916589587 1103 100-101 0.40221370299962445 1104 1 0.013504693450016703 1104 2 -0.002235349859489588 1104 3 -0.04678473303545161 1104 4 -0.019396214681613344 1104 5 -0.1257379483306309 1104 6 0.04281331254724563 1104 7 -0.2047720938394164 1104 8 -0.2113665759647958 1104 9 -0.18708795524683097 1104 10-11 -0.06935560695044529 1104 12-13 -0.10389894252170251 1104 14-15 -0.09412302042861143 1104 16-17 0.029582645676093478 1104 18-19 -0.2029590474116958 1104 20-21 -0.18592349238446815 1104 22-23 -0.17471378387737246 1104 24-25 -0.17572568565007884 1104 26-27 -0.13853144418546748 1104 28-29 -0.21425721576795098 1104 30-31 -0.27807583900030153 1104 32-33 -0.24173599032626214 1104 34-35 -0.3869216611810202 1104 36-37 -0.2234304690191351 1104 38-39 -0.4384882955972813 1104 40-41 -0.31118087401563344 1104 42-43 -0.528214733115739 1104 44-45 -0.5617822180019019 1104 46-47 -0.5932615606105642 1104 48-49 -0.7578555813535175 1104 50-51 -0.687065608374489 1104 52-53 -0.6386091160904215 1104 54-55 -0.605591848046501 1104 56-57 -0.5844216075963615 1104 58-59 -0.860863411880203 1104 60-61 -0.6928032771989834 1104 62-63 -0.9288673185004797 1104 64-65 -0.7639675121901988 1104 66-67 -0.8596330487230901 1104 68-69 -0.6117680472646505 1104 70-71 -0.7962023923322725 1104 72-73 -0.8144735222059794 1104 74-75 -0.596461523285317 1104 76-77 -0.751128865580899 1104 78-79 -0.5608957988958672 1104 80-81 -0.32802860097573827 1104 82-83 -0.1977272157834271 1104 84-85 -0.3341216700898748 1104 86-87 -0.455628796632908 1104 88-89 -0.12486703600946214 1104 90-91 -0.21016692369035184 1104 92-93 -0.3772958471123218 1104 94-95 -0.3985454112934441 1104 96-97 -0.0013157160000609736 1104 98-99 0.30557541129652677 1104 100-101 0.8099698301514344 1105 1 0.06938973558310835 1105 2 0.16942767186191787 1105 3 0.09254329968256769 1105 4 -0.07896790272371845 1105 5 -0.10909630205094345 1105 6 -0.03638547906073342 1105 7 -0.09903470287888183 1105 8 -0.1611945221925879 1105 9 -0.11670498887917091 1105 10-11 0.05735798066415754 1105 12-13 -0.23880207346790172 1105 14-15 0.07688591895409047 1105 16-17 0.13666140738316557 1105 18-19 0.03864599496851895 1105 20-21 0.06938484527337607 1105 22-23 -0.0011549272781650188 1105 24-25 0.03496373412405518 1105 26-27 -0.22099524184775987 1105 28-29 -0.061140942014027644 1105 30-31 0.11049463200076559 1105 32-33 0.06848437441537669 1105 34-35 0.10514594946469913 1105 36-37 -0.03790584401604491 1105 38-39 -0.024945316132530593 1105 40-41 -0.14994533023921974 1105 42-43 0.09217657338091101 1105 44-45 0.4064681464926281 1105 46-47 0.21138404568593216 1105 48-49 0.40007402795777125 1105 50-51 0.3273010360518356 1105 52-53 0.1372591840059556 1105 54-55 0.23299962772388483 1105 56-57 0.39503850805343177 1105 58-59 0.3529906235066562 1105 60-61 0.3055881590330003 1105 62-63 0.28015021430402953 1105 64-65 0.03851892737784368 1105 66-67 0.06672438449410123 1105 68-69 0.038161216085732974 1105 70-71 0.14547848344305336 1105 72-73 0.031169223735158624 1105 74-75 -0.11322518256325154 1105 76-77 -0.24990796336427934 1105 78-79 0.009877145725678105 1105 80-81 0.30844293756183205 1105 82-83 0.14888159509951393 1105 84-85 0.17969437137601219 1105 86-87 0.1075492249649379 1105 88-89 0.19609587821694419 1105 90-91 0.28652019173703636 1105 92-93 0.08010153841168233 1105 94-95 0.2680224093999577 1105 96-97 0.6016439486649752 1105 98-99 0.9019602742712927 1105 100-101 1.260941455179232 1106 1 -0.029655922487943798 1106 2 0.07906546314864471 1106 3 0.07295600354242282 1106 4 0.04002456385796904 1106 5 -0.07527149896567664 1106 6 -0.1135315227905096 1106 7 0.008520776416474973 1106 8 -0.0984820830645674 1106 9 -0.005266367445699416 1106 10-11 -0.017634052868537253 1106 12-13 -0.14351903918349507 1106 14-15 -0.06677297734293575 1106 16-17 0.15149639937583714 1106 18-19 0.020074934080199114 1106 20-21 0.0754005494273784 1106 22-23 0.0184599450949392 1106 24-25 -0.00862816214028328 1106 26-27 -0.20681288736856374 1106 28-29 -0.20329846562746212 1106 30-31 0.035063421424560204 1106 32-33 0.10081393286882445 1106 34-35 0.26676310147306026 1106 36-37 0.22258129524597337 1106 38-39 0.3647037916231284 1106 40-41 0.23036871198713982 1106 42-43 0.16289837617203773 1106 44-45 0.13669292343153927 1106 46-47 0.07816326423676401 1106 48-49 0.21413872384299282 1106 50-51 0.12961417132652997 1106 52-53 0.1603680672297969 1106 54-55 0.290571652759013 1106 56-57 0.4946499895712293 1106 58-59 0.28738880796918664 1106 60-61 0.3702891509603248 1106 62-63 0.24218284822238445 1106 64-65 0.15849698895250697 1106 66-67 0.06571631997797311 1106 68-69 0.029571730188422407 1106 70-71 0.2886951386706862 1106 72-73 0.3526427342100398 1106 74-75 0.4303452655198967 1106 76-77 -0.22181186734179192 1106 78-79 0.003020877137252853 1106 80-81 0.09938138740985991 1106 82-83 0.3654214110945446 1106 84-85 -0.02073888188411388 1106 86-87 0.16768992503357083 1106 88-89 0.09342752020798883 1106 90-91 0.06634950506840553 1106 92-93 -0.21767275318553203 1106 94-95 0.09504793477826468 1106 96-97 0.1912023612544438 1106 98-99 0.7421457908252052 1106 100-101 0.8002661847278816 1107 1 -0.06823624426011676 1107 2 0.049992781444380796 1107 3 -0.05256748259227351 1107 4 -0.052222182039031395 1107 5 -0.041833064169004786 1107 6 -0.011615442266617038 1107 7 -0.06372435253493336 1107 8 -0.0372483535795638 1107 9 -0.06376745888397295 1107 10-11 -0.24084530680984528 1107 12-13 -0.22456035214332104 1107 14-15 -0.1420660487310812 1107 16-17 -0.22654547951493242 1107 18-19 -0.08283556798140523 1107 20-21 -0.05718090420671018 1107 22-23 -0.18934181737575528 1107 24-25 -0.1496177328412287 1107 26-27 -0.09164593773641627 1107 28-29 -0.08419425348419196 1107 30-31 -0.06810579193140853 1107 32-33 -0.1372247121307737 1107 34-35 -0.08013640535853028 1107 36-37 0.044684941458278615 1107 38-39 0.2498117227412422 1107 40-41 0.13169460737076122 1107 42-43 -0.03645484559205414 1107 44-45 0.17244359042159374 1107 46-47 0.4092813821120984 1107 48-49 0.2599144534896851 1107 50-51 0.4080441171588589 1107 52-53 0.25862176599283515 1107 54-55 0.26028626186241866 1107 56-57 -0.03292701746249094 1107 58-59 0.1625121000704084 1107 60-61 0.5439410225748347 1107 62-63 0.27408518470876686 1107 64-65 0.38027724604685886 1107 66-67 0.48293679050913596 1107 68-69 0.5830360361145281 1107 70-71 0.7308408254683236 1107 72-73 0.6818771104732804 1107 74-75 0.41103893517791334 1107 76-77 -0.01331481826749581 1107 78-79 0.22091238008817626 1107 80-81 0.31592274079331517 1107 82-83 0.1953591586893424 1107 84-85 0.32384030802614916 1107 86-87 0.08326111106316247 1107 88-89 -0.11899870664947798 1107 90-91 -0.026139782667062406 1107 92-93 0.10468960432113761 1107 94-95 0.42908463944315045 1107 96-97 0.7649108259646091 1107 98-99 0.9007034753591192 1107 100-101 1.1215047650496537 1108 1 0.11132376687884005 1108 2 0.19017657425975187 1108 3 0.20494667316749826 1108 4 0.1300854388650805 1108 5 0.03333634082495962 1108 6 0.16399023860222428 1108 7 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0.08921642220167669 2316 90-91 -0.33631495675002654 2316 92-93 -0.09157083569711233 2316 94-95 0.11265834944374475 2316 96-97 0.11762771052429244 2316 98-99 0.13643263995927768 2316 100-101 -0.7075804810471169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 17.0 6 35.0 7 86.0 8 240.0 9 381.0 10 608.0 11 1093.0 12 1590.0 13 2350.0 14 2890.0 15 3934.0 16 5408.0 17 7281.0 18 6615.0 19 4014.0 20 3441.0 21 3503.0 22 3723.0 23 4027.0 24 4727.0 25 5495.0 26 6783.0 27 8363.0 28 10492.0 29 13060.0 30 16166.0 31 20255.0 32 25812.0 33 33940.0 34 45488.0 35 63657.0 36 92555.0 37 123024.0 38 76829.0 39 4569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.159995483355665 19.435685143395155 14.848302190074342 21.556017183174838 2 15.898255678837739 20.919901993984183 39.75584815707522 23.425994170102854 3 20.082964546682913 25.31779836859631 29.80000863176556 24.79922845295522 4 13.218436594329194 17.033499653085546 36.809449352149706 32.938614400435554 5 13.573386051313715 38.0583649126651 33.68755301261015 14.680696023411032 6 32.001288058732825 36.16955234979426 17.111594686425754 14.717564905047166 7 28.836746854962264 33.272582109594545 20.20343360604989 17.687237429393303 8 27.251405494500027 34.30717876960514 19.124684417311112 19.31673131858373 9 27.21034573766165 16.786759421098147 20.102049793261195 35.900845047979004 10-11 25.466176670749967 26.894750470575346 26.506422067078983 21.132650791595704 12-13 26.628350900489668 24.23130550253133 27.483940575981407 21.656403020997594 14-15 23.37437712259688 25.51390498069537 26.316964508022295 24.794753388685457 16-17 22.047717892259183 28.79930083917558 27.01064759522373 22.142333673341504 18-19 21.78603618183538 26.94449562375406 30.19759086540616 21.071877329004394 20-21 22.802125296909157 25.790471353142213 30.30863613502706 21.098767214921562 22-23 22.321521199842973 25.839515773625997 30.204640917726167 21.634322108804863 24-25 22.37155737922609 25.951285422144316 30.514749820731417 21.162407377898177 26-27 22.040114736845602 26.081301811686824 30.447344220496596 21.43123923097098 28-29 21.595073184294943 26.47491150240489 30.649176028252832 21.28083928504733 30-31 21.883014163854533 26.15723488627253 30.325554528267123 21.63419642160581 32-33 21.86936938806167 26.18230920343362 30.27440246524841 21.673918943256297 34-35 22.247417820112283 26.227095707489067 30.067579905643775 21.45790656675487 36-37 22.576535058958683 25.979421320083468 29.996712989171126 21.447330631786723 38-39 22.18551676397302 26.24756595777175 30.210759421175215 21.356157857080014 40-41 22.186857046582713 26.283416978659446 30.115471217680636 21.41425475707721 42-43 22.174694270502656 26.461173287520594 29.621256447603756 21.742875994373 44-45 22.322433396962403 26.395717486928376 29.511743713171217 21.770105402938004 46-47 22.8540981797026 26.8433488558925 28.78647669997751 21.51607626442739 48-49 22.802933350596145 27.062449892190404 28.469120310199504 21.66549644701395 50-51 22.609077426278176 27.157417912343007 28.45163212579291 21.78187253558591 52-53 22.812683109825226 27.402857384731067 28.09736753430638 21.687091971137328 54-55 22.44266002697375 27.40516651104886 28.140180516651103 22.011992945326277 56-57 22.689604287856742 27.629968187993768 27.961616444973032 21.718811079176454 58-59 22.815031583455266 27.11886351017468 27.982418501738316 22.08368640463174 60-61 22.565432641831244 27.235084428545935 28.495142609337975 21.704340320284846 62-63 22.452985536692776 27.177920424420794 28.699032736242597 21.67006130264383 64-65 22.410116756708305 27.462934081886566 28.257912223722688 21.869036937682445 66-67 22.260142088838723 27.54656065334307 28.030343270699156 22.16295398711905 68-69 22.361056241472152 27.33741895431563 28.16879081332851 22.13273399088371 70-71 22.525609732818886 27.504060459136888 27.880934142133086 22.089395665911148 72-73 22.44097821053138 27.294766178601606 28.035412243732644 22.22884336713437 74-75 22.496642713318426 27.75924146070605 27.54543749304888 22.19867833292664 76-77 22.610938709926405 27.59136409774608 27.721003886703787 22.076693305623728 78-79 22.61146628975218 27.535312909016817 27.552327178594222 22.30089362263678 80-81 22.730441711070164 27.765134538452603 27.321265541224705 22.183158209252525 82-83 22.77215560736455 27.801399838818515 27.379854637868682 22.046589915948253 84-85 22.49526704176208 27.693262807206548 27.3358736239977 22.475596527033677 86-87 22.52730790267174 27.672672012190517 27.30532978219245 22.494690302945294 88-89 22.51285694839624 27.583341910165704 27.278477020176027 22.62532412126202 90-91 22.164590341915034 27.612753605866004 27.634583444350096 22.588072607868867 92-93 22.325033633412815 27.498977096081596 27.607699540958897 22.568289729546695 94-95 22.097886195325877 27.607937328666786 27.569925528409673 22.724250947597664 96-97 22.2749605776413 27.64262594406175 27.45597144991286 22.626442028384098 98-99 22.04448103659058 27.69777395625929 27.15647209290938 23.101272914240752 100-101 21.597215939790466 28.11781295570685 27.050551143328804 23.23441996117388 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 162.0 1 123.0 2 124.0 3 168.0 4 153.0 5 141.5 6 199.0 7 371.5 8 604.0 9 761.5 10 752.0 11 720.5 12 763.0 13 817.0 14 910.5 15 989.5 16 1056.5 17 1106.5 18 1179.5 19 1276.5 20 1374.5 21 1512.0 22 1726.0 23 2009.5 24 2246.5 25 2578.0 26 3122.0 27 3904.5 28 4660.5 29 5551.0 30 6755.0 31 7999.0 32 9444.0 33 10824.5 34 12241.0 35 13642.0 36 15379.5 37 16788.0 38 17605.5 39 18308.5 40 18373.0 41 18682.0 42 19028.0 43 19415.0 44 20355.0 45 21259.5 46 21613.5 47 22410.5 48 25056.5 49 24881.5 50 22237.5 51 20895.0 52 20139.0 53 19807.0 54 19174.5 55 17888.5 56 15951.5 57 14212.0 58 13362.0 59 12252.0 60 10176.5 61 8257.5 62 6776.5 63 5174.5 64 3628.0 65 2635.5 66 2009.5 67 1600.5 68 1221.5 69 959.0 70 772.5 71 558.0 72 429.5 73 343.0 74 245.0 75 208.0 76 169.5 77 86.0 78 58.0 79 60.5 80 45.0 81 30.5 82 16.5 83 11.0 84 8.5 85 7.5 86 4.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03983686802543584 2 0.00746941275476922 3 0.005145595453285463 4 4.97960850317948E-4 5 9.95921700635896E-4 6 0.0 7 0.0 8 0.0 9 9.95921700635896E-4 10-11 4.97960850317948E-4 12-13 0.0011619086507418787 14-15 0.0018258564511658092 16-17 0.0034027324771726446 18-19 0.0 20-21 0.0 22-23 0.0019088499262188007 24-25 0.001493882550953844 26-27 0.005809543253709393 28-29 0.006556484529186316 30-31 0.0013278956008478614 32-33 4.1496737526495667E-4 34-35 0.009793230056252978 36-37 0.014274877709114508 38-39 0.010623164806782891 40-41 0.0020748368763247836 42-43 7.46941275476922E-4 44-45 0.0011619086507418787 46-47 8.299347505299134E-5 48-49 9.95921700635896E-4 50-51 0.0010789151756888872 52-53 0.002987765101907688 54-55 0.003236745527066662 56-57 0.0019088499262188007 58-59 9.129282255829046E-4 60-61 0.0040666802775965755 62-63 0.005975530203815376 64-65 0.008382340980352124 66-67 0.0028217781518017054 68-69 0.0031537520520136704 70-71 8.299347505299134E-5 72-73 0.0016598695010598267 74-75 0.005975530203815376 76-77 0.003070758576960679 78-79 0.003568719427278627 80-81 0.004481647652861532 82-83 0.005062601978232471 84-85 0.0053945758784444365 86-87 0.003568719427278627 88-89 0.009793230056252978 90-91 0.011702079982471778 92-93 9.129282255829046E-4 94-95 0.0020748368763247836 96-97 0.0011619086507418787 98-99 0.0034027324771726446 100-101 0.004564641127914523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 602457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.09109088282638 #Duplication Level Percentage of deduplicated Percentage of total 1 85.50768148768033 57.36803629869732 2 8.818564354964398 11.83294205189939 3 2.338865137890064 4.707510405865696 4 0.9770706087177868 2.622109320336941 5 0.5848243891166894 1.9618253120360611 6 0.3404994585638641 1.3706688072036828 7 0.24723394520032543 1.1611036560728323 8 0.175762245552162 0.9433664632087797 9 0.1319944343864276 0.797008553410237 >10 0.796187772925543 9.900990272335038 >50 0.052062201601684926 2.3733485351241255 >100 0.027766473736251036 3.253225790172396 >500 4.958298881454948E-4 0.22204900198734007 >1k 9.916597762909896E-4 1.485815531650119 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3206 0.5321541620397804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2738 0.4544722693901805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1698 0.28184584127995854 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1291 0.21428915258682363 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.1359433121367998 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6598695010598268E-4 2 0.0 0.0 0.0 0.0 1.6598695010598268E-4 3 0.0 0.0 0.0 0.0 1.6598695010598268E-4 4 0.0 0.0 0.0 0.0 1.6598695010598268E-4 5 0.0 0.0 0.0 0.0 1.6598695010598268E-4 6 0.0 0.0 0.0 0.0 1.6598695010598268E-4 7 0.0 0.0 0.0 0.0 1.6598695010598268E-4 8 0.0 0.0 0.0 0.0 1.6598695010598268E-4 9 0.0 0.0 0.0 0.0 1.6598695010598268E-4 10-11 0.0 0.0 0.0 8.299347505299134E-5 1.6598695010598268E-4 12-13 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 14-15 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 16-17 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 18-19 0.0 0.0 0.0 1.6598695010598268E-4 1.6598695010598268E-4 20-21 0.0 0.0 0.0 2.48980425158974E-4 1.6598695010598268E-4 22-23 0.0 0.0 0.0 4.97960850317948E-4 1.6598695010598268E-4 24-25 0.0 0.0 0.0 9.95921700635896E-4 1.6598695010598268E-4 26-27 0.0 0.0 0.0 0.0020748368763247836 1.6598695010598268E-4 28-29 0.0 0.0 0.0 0.0035687194272786276 1.6598695010598268E-4 30-31 0.0 0.0 0.0 0.006141517153921359 1.6598695010598268E-4 32-33 0.0 0.0 0.0 0.010291190906570925 1.6598695010598268E-4 34-35 0.0 0.0 0.0 0.013942903808902543 1.6598695010598268E-4 36-37 0.0 0.0 0.0 0.019586460112505956 1.6598695010598268E-4 38-39 0.0 0.0 0.0 0.028964722793493974 1.6598695010598268E-4 40-41 0.0 1.6598695010598268E-4 0.0 0.03842597894953499 1.6598695010598268E-4 42-43 0.0 2.48980425158974E-4 0.0 0.05211990233327855 1.6598695010598268E-4 44-45 0.0 3.3197390021196536E-4 0.0 0.0661457996172341 1.6598695010598268E-4 46-47 0.0 3.3197390021196536E-4 0.0 0.09527650936083405 1.6598695010598268E-4 48-49 0.0 3.3197390021196536E-4 0.0 0.12797593853171263 1.6598695010598268E-4 50-51 0.0 3.3197390021196536E-4 0.0 0.14108890759008524 1.6598695010598268E-4 52-53 0.0 3.3197390021196536E-4 0.0 0.15926447862669035 1.6598695010598268E-4 54-55 0.0 3.3197390021196536E-4 0.0 0.19503466637452962 1.6598695010598268E-4 56-57 0.0 3.3197390021196536E-4 0.0 0.25279812501141163 1.6598695010598268E-4 58-59 0.0 3.3197390021196536E-4 0.0 0.3265793243335209 1.6598695010598268E-4 60-61 0.0 3.3197390021196536E-4 0.0 0.4103197406619892 1.6598695010598268E-4 62-63 0.0 3.3197390021196536E-4 0.0 0.49870779159342493 1.6598695010598268E-4 64-65 0.0 3.3197390021196536E-4 0.0 0.5869298555747546 1.6598695010598268E-4 66-67 0.0 3.3197390021196536E-4 0.0 0.7079343422020161 1.6598695010598268E-4 68-69 0.0 3.3197390021196536E-4 0.0 0.8625511862257389 1.6598695010598268E-4 70-71 0.0 3.3197390021196536E-4 0.0 0.9429718635520876 1.6598695010598268E-4 72-73 0.0 3.3197390021196536E-4 0.0 1.0582498004006924 1.6598695010598268E-4 74-75 0.0 3.3197390021196536E-4 0.0 1.177926391427106 2.48980425158974E-4 76-77 0.0 3.3197390021196536E-4 0.0 1.3081431537852495 3.3197390021196536E-4 78-79 0.0 3.3197390021196536E-4 0.0 1.4442524528721552 3.3197390021196536E-4 80-81 0.0 3.3197390021196536E-4 0.0 1.665679044313536 3.3197390021196536E-4 82-83 0.0 3.3197390021196536E-4 0.0 1.9180622019496827 3.3197390021196536E-4 84-85 0.0 3.3197390021196536E-4 0.0 2.3034009066207215 3.3197390021196536E-4 86-87 0.0 3.3197390021196536E-4 0.0 2.825263877753931 3.3197390021196536E-4 88-89 0.0 3.3197390021196536E-4 0.0 3.3740997282793628 3.3197390021196536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 545 0.0 30.503044 2 TATCACG 70 0.00839053 27.141483 2 AGTGCGA 35 0.008395502 27.140356 68-69 GTACCTG 605 0.0 26.695078 1 GGTATCA 3690 0.0 25.874819 1 GTAAGGT 205 9.567884E-10 25.484396 4 TAAGGTG 245 1.0913936E-11 25.20071 5 GTATCAA 5055 0.0 25.1838 1 GTACATG 3285 0.0 23.714619 1 GAGTACT 3230 0.0 22.870285 12-13 ATCACGC 105 0.0021956635 22.6179 3 AGTACTT 3310 0.0 22.532812 12-13 ACATGGG 3205 0.0 22.52616 3 TACATGG 3405 0.0 22.318987 2 GTACTTT 3325 0.0 22.143572 14-15 GTATAGT 155 3.9014783E-5 21.452307 1 ATCAACG 5995 0.0 20.995602 3 TCAACGC 6000 0.0 20.97636 4 TATCAAC 6005 0.0 20.960636 2 CAACGCA 6080 0.0 20.778473 5 >>END_MODULE