FastQCFastQC Report
Thu 26 May 2016
SRR936524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651917
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.3600151553035126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.30080516384754497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.17210779899895232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8180.12547609588337166No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7160.10982993233801236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC500.001608909138.0306853
ACGGTAG650.005819238329.2543723
GTATCAA39850.028.8667471
GGTATCA27750.026.893511
GTACATG33850.025.9790861
TACATGG34000.025.5848562
GCCGCGC950.001221321425.0163468
TCAACGC46100.024.8538484
CAACGCA46450.024.5642225
ATCAACG46500.024.5359253
ACATGGG33800.024.4724083
TATCAAC46800.024.3767722
AACGCAG47700.024.1198436
TACCTGG4950.024.0074272
GAGTACT23000.023.34862912-13
GTAGTGC1302.9220947E-421.9424656
ACGCAGA52000.021.6681827
CGCAGAG52150.021.6008828
CCGCGCC1553.8857554E-521.463999
TCCACGA2005.341044E-721.3906172
Produced by FastQC (version 0.11.5)