FastQCFastQC Report
Thu 26 May 2016
SRR936524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651917
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.50374510865647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.4543523178564142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.2836250626996995No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13740.21076302658160473No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.13452632773804027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG500.001616437537.994572
TACCTGG6150.032.434392
GCACCGT1252.155648E-730.3933266
GTACCTG6800.029.3407961
TAGACTG2950.028.9766865
GTGTAGG2600.027.4062371
GGTATCA40350.026.0183751
GTATCAA55750.025.7331851
ACCGTGG1901.1918019E-824.9945138
GAGTACT35250.022.63506312-13
GTACATG35500.022.4808331
ACGAATC853.1534346E-622.34974772-73
GCCGCGC1503.0357589E-522.1617988
AGTACTT36500.021.9249512-13
ACATGGG34500.021.6111973
TACATGG35500.021.5391752
GTACTTT37100.021.44235214-15
TCAACGC65950.021.3865074
ATCAACG66200.021.3057443
ACCTGGG9150.021.279483
Produced by FastQC (version 0.11.5)