##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936524_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651917 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.075439051290275 31.0 31.0 34.0 31.0 34.0 2 32.23974524364298 33.0 31.0 34.0 31.0 34.0 3 32.31483609109748 34.0 31.0 34.0 30.0 34.0 4 35.99397929491024 37.0 35.0 37.0 35.0 37.0 5 35.85155012064419 37.0 35.0 37.0 35.0 37.0 6 35.94573849124966 37.0 35.0 37.0 35.0 37.0 7 35.896713845474196 37.0 35.0 37.0 35.0 37.0 8 35.86338138137217 37.0 35.0 37.0 35.0 37.0 9 37.53674010648595 39.0 37.0 39.0 35.0 39.0 10-11 37.432831173293536 39.0 37.0 39.0 34.5 39.0 12-13 37.331613533624676 39.0 37.0 39.0 34.0 39.0 14-15 38.49491729775416 40.0 38.0 41.0 34.0 41.0 16-17 38.39741638889613 40.0 38.0 41.0 34.0 41.0 18-19 38.54326547704693 40.0 38.0 41.0 34.0 41.0 20-21 38.56263910896632 40.0 38.0 41.0 34.0 41.0 22-23 38.48016541983105 40.0 38.0 41.0 34.0 41.0 24-25 38.35221968440768 40.0 38.0 41.0 34.0 41.0 26-27 38.15632434803817 40.0 38.0 41.0 33.5 41.0 28-29 38.083142485929955 40.0 37.5 41.0 33.5 41.0 30-31 38.05151115862909 40.0 37.0 41.0 33.0 41.0 32-33 37.88305643203046 40.0 37.0 41.0 33.0 41.0 34-35 37.711187927297495 40.0 37.0 41.0 33.0 41.0 36-37 37.515375423558524 40.0 36.5 41.0 33.0 41.0 38-39 37.336100301111955 40.0 36.0 41.0 32.5 41.0 40-41 37.15327104524042 39.0 36.0 41.0 32.0 41.0 42-43 36.8799992943887 39.0 36.0 41.0 31.0 41.0 44-45 36.55055398156514 39.0 35.0 41.0 30.5 41.0 46-47 36.124820337558305 39.0 35.0 40.5 30.0 41.0 48-49 35.69034401618611 39.0 35.0 40.0 28.5 41.0 50-51 35.39989676599935 39.0 35.0 40.0 27.5 41.0 52-53 35.03147639960302 38.0 34.5 40.0 26.0 41.0 54-55 34.860912508800965 38.0 34.0 40.0 26.0 41.0 56-57 34.55301671838593 38.0 34.0 40.0 26.0 41.0 58-59 34.198960143699274 38.0 33.5 40.0 25.0 41.0 60-61 33.78512602064373 37.0 33.0 40.0 23.0 41.0 62-63 33.45097228634934 36.5 33.0 39.0 23.0 41.0 64-65 33.021419137712314 36.0 32.0 39.0 22.0 40.0 66-67 32.61532219592371 35.5 32.0 39.0 21.5 40.0 68-69 32.246888791057756 35.0 31.0 38.0 20.0 40.0 70-71 31.8822802596036 35.0 31.0 38.0 19.5 40.0 72-73 31.434825292176765 35.0 31.0 37.0 18.0 39.0 74-75 30.928760102896533 34.0 30.0 36.5 15.0 39.0 76-77 29.451493825134182 33.0 28.5 35.0 12.5 37.5 78-79 30.1950892521594 34.0 30.0 36.0 11.0 37.5 80-81 29.958671886145016 34.0 30.0 35.0 7.0 37.0 82-83 29.680745401638553 34.0 29.5 35.0 7.0 37.0 84-85 29.329527378485295 34.0 29.0 35.0 2.0 36.0 86-87 28.962748325323624 34.0 29.0 35.0 2.0 36.0 88-89 28.50460948249547 33.5 29.0 35.0 2.0 35.5 90-91 28.043757871017323 33.0 27.5 35.0 2.0 35.0 92-93 27.38726555681168 33.0 26.5 35.0 2.0 35.0 94-95 26.891951736187274 33.0 25.5 34.0 2.0 35.0 96-97 26.251430013329916 32.0 25.0 34.0 2.0 35.0 98-99 25.44353498988368 32.0 22.0 34.0 2.0 35.0 100-101 22.27225551719007 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18867429008712833 1101 2 0.2548605094476599 1101 3 0.2583254040438092 1101 4 0.22184614923315849 1101 5 0.23503965834225937 1101 6 0.22774684368476272 1101 7 0.2522567126570152 1101 8 0.1280319791906166 1101 9 0.18987172383033624 1101 10-11 0.24473230323825135 1101 12-13 0.26284959370683936 1101 14-15 0.43470331732793 1101 16-17 0.37779327066974844 1101 18-19 0.3667368156014632 1101 20-21 0.2191024028215196 1101 22-23 0.11364927436749639 1101 24-25 0.17856685988710552 1101 26-27 0.19509996669645346 1101 28-29 0.28576202429977116 1101 30-31 0.16421240653810543 1101 32-33 0.22248433264494594 1101 34-35 0.3757600028152055 1101 36-37 0.2836675961759525 1101 38-39 0.31589170041890213 1101 40-41 0.33856939734054237 1101 42-43 0.5074210266114676 1101 44-45 0.4885657828205652 1101 46-47 0.5340130378709063 1101 48-49 0.4895830474892122 1101 50-51 0.5038554461532954 1101 52-53 0.34356752652140443 1101 54-55 0.45908182779390216 1101 56-57 0.3853980405910846 1101 58-59 0.48166417677270346 1101 60-61 0.5375234067245671 1101 62-63 0.34698976842742013 1101 64-65 0.37423692740966175 1101 66-67 0.048930168149375675 1101 68-69 0.10836050827020927 1101 70-71 0.2234140146705279 1101 72-73 0.478182854228141 1101 74-75 0.3375373582994463 1101 76-77 0.1697697546568655 1101 78-79 0.06942298947629055 1101 80-81 0.10604963076914231 1101 82-83 0.0991627033744713 1101 84-85 -0.02862285167213585 1101 86-87 0.19759207123080458 1101 88-89 0.13916533655424956 1101 90-91 -0.09513292288265163 1101 92-93 0.08379730495680349 1101 94-95 0.07765995746047238 1101 96-97 0.11752942402069877 1101 98-99 -0.16276452723563395 1101 100-101 0.3565685513526269 1102 1 0.15530686112043668 1102 2 0.2304024564240521 1102 3 0.07366060507006011 1102 4 0.15691043686143047 1102 5 0.18276117090645272 1102 6 0.16379037926840567 1102 7 0.14640713048431309 1102 8 0.08115359911599995 1102 9 0.1734648694507328 1102 10-11 0.17307538899099484 1102 12-13 0.11081333660617787 1102 14-15 0.29232199995456654 1102 16-17 0.1765702789960315 1102 18-19 0.08227552215735301 1102 20-21 0.09863799002755513 1102 22-23 0.06863191052079287 1102 24-25 0.07630132523107847 1102 26-27 0.08239075259635342 1102 28-29 0.1284293710986546 1102 30-31 0.18578345854417933 1102 32-33 0.041987569059443786 1102 34-35 0.03708086587093362 1102 36-37 0.013578992040500282 1102 38-39 0.011970079425246638 1102 40-41 0.1452867712467878 1102 42-43 0.049331503763383466 1102 44-45 -0.11900816597900388 1102 46-47 -0.10141695435742548 1102 48-49 0.08701376792193827 1102 50-51 -0.19997768643420955 1102 52-53 -0.08376025714670021 1102 54-55 -0.1363534523733918 1102 56-57 -0.04584628978580696 1102 58-59 -0.15878134543797984 1102 60-61 0.05885142719262859 1102 62-63 0.05409804031294385 1102 64-65 0.06831986615058128 1102 66-67 0.031035747103018707 1102 68-69 -0.08721133247558299 1102 70-71 0.08701100606472068 1102 72-73 0.3143306352077637 1102 74-75 -0.08623174927628341 1102 76-77 -0.1315675419600133 1102 78-79 -0.045394278404110366 1102 80-81 0.1760200129696159 1102 82-83 0.12306436551434174 1102 84-85 0.09968934325874557 1102 86-87 -0.09648454690967512 1102 88-89 -0.14741246781159134 1102 90-91 -0.13770127661549836 1102 92-93 -0.0934692757140887 1102 94-95 -0.2959627525459254 1102 96-97 -0.041089159790910657 1102 98-99 -0.11324306790691807 1102 100-101 0.4793573427024853 1103 1 0.21759110264259363 1103 2 0.2733349907493192 1103 3 0.21543901321197012 1103 4 0.26312843355232474 1103 5 0.2701977410283618 1103 6 0.2006039316460999 1103 7 0.21570453183176141 1103 8 0.17989242996689114 1103 9 0.3501992654008177 1103 10-11 0.28011558639298784 1103 12-13 0.354742108740318 1103 14-15 0.41055280119884685 1103 16-17 0.3112340210520088 1103 18-19 0.2519109393526975 1103 20-21 0.28572510177765054 1103 22-23 0.09654520940967615 1103 24-25 0.2221270648195386 1103 26-27 0.058058779139315675 1103 28-29 0.2517156455143663 1103 30-31 0.3009090909973935 1103 32-33 0.37246427014288486 1103 34-35 0.22643064444886818 1103 36-37 0.09090046329069423 1103 38-39 0.46873579467065696 1103 40-41 0.38384586219363115 1103 42-43 0.47268966306731386 1103 44-45 0.6306824706221192 1103 46-47 0.7852831417044044 1103 48-49 0.5251254008162434 1103 50-51 0.5824173445574985 1103 52-53 0.6081400969255881 1103 54-55 0.6609874527118009 1103 56-57 0.5803836661881547 1103 58-59 0.579059358018867 1103 60-61 0.497674988087617 1103 62-63 0.6282423656054377 1103 64-65 0.45950792310851085 1103 66-67 0.29542771998792716 1103 68-69 0.5096713179820753 1103 70-71 0.5320762792042224 1103 72-73 0.5674904789619468 1103 74-75 0.3757057099481891 1103 76-77 0.3182099286331628 1103 78-79 0.39562274050465973 1103 80-81 0.2854700612773726 1103 82-83 0.37033177083899105 1103 84-85 0.7072522979029792 1103 86-87 0.5667979625465556 1103 88-89 0.34354101740586884 1103 90-91 0.32175112479193047 1103 92-93 0.390291568153593 1103 94-95 0.24795485389953598 1103 96-97 0.6882227108125996 1103 98-99 0.6893198728955525 1103 100-101 1.2342963143702441 1104 1 0.09787676508598508 1104 2 0.09097174740372793 1104 3 -0.004305499954853076 1104 4 0.13338415705695894 1104 5 0.13607109813402474 1104 6 0.09634719834326688 1104 7 0.13513663868103265 1104 8 0.17349638395796063 1104 9 0.07715634108835445 1104 10-11 0.1414128023477943 1104 12-13 0.08023280755222117 1104 14-15 0.14744514724407054 1104 16-17 0.33803213241382224 1104 18-19 0.08146361058449969 1104 20-21 0.1364697293356656 1104 22-23 0.16343940938126877 1104 24-25 0.2298277622114142 1104 26-27 0.2653257760941443 1104 28-29 0.3030777883377027 1104 30-31 0.17022010095337237 1104 32-33 0.07361528230779157 1104 34-35 0.08657014254760753 1104 36-37 0.15268749053947062 1104 38-39 0.27742390508578296 1104 40-41 0.3481079797720028 1104 42-43 0.08563569014252437 1104 44-45 -0.02584364607267986 1104 46-47 -0.12258657854993515 1104 48-49 0.11538597608565482 1104 50-51 0.0995523577322075 1104 52-53 0.19819654306705559 1104 54-55 7.603861755640651E-4 1104 56-57 -0.04667981303997948 1104 58-59 -0.03421554376247826 1104 60-61 0.10260771422719017 1104 62-63 0.0123226603402955 1104 64-65 -0.1703394040818793 1104 66-67 0.03708554434614797 1104 68-69 -0.139262575736673 1104 70-71 -0.22719779028251352 1104 72-73 -0.2354346815801911 1104 74-75 -0.11239661889890229 1104 76-77 -0.41054831542243875 1104 78-79 -0.382351825203223 1104 80-81 -0.044101346444431755 1104 82-83 0.0869541930924207 1104 84-85 0.13152334982869363 1104 86-87 0.21783034314580974 1104 88-89 0.21727630433056078 1104 90-91 0.3433079496077234 1104 92-93 0.25170070998290583 1104 94-95 0.4952094650483403 1104 96-97 0.33623851652111725 1104 98-99 0.3749265012740395 1104 100-101 1.2149020749534039 1105 1 -0.0458014469263901 1105 2 0.07488056999183357 1105 3 0.09270607502470085 1105 4 0.055939809416400976 1105 5 0.10180622490761237 1105 6 0.027717437666197497 1105 7 -0.12799634023151896 1105 8 -0.039685046289896775 1105 9 -0.125376908084867 1105 10-11 -0.17237293606833504 1105 12-13 0.027972041576617812 1105 14-15 -0.0054388184755040925 1105 16-17 0.04636176151662852 1105 18-19 0.03303338939748102 1105 20-21 -0.04008093564779358 1105 22-23 -0.02620756263252133 1105 24-25 0.047306248040506205 1105 26-27 -0.05291059530090081 1105 28-29 -0.19751229558534078 1105 30-31 -0.07493254297820329 1105 32-33 -0.025474365997744997 1105 34-35 0.013454188278551271 1105 36-37 -0.07474041031753842 1105 38-39 -0.14187239483242564 1105 40-41 0.22131994769633678 1105 42-43 0.06496719506766624 1105 44-45 0.14098563631414862 1105 46-47 0.2590899265530098 1105 48-49 0.08008762254203816 1105 50-51 0.13500628895798172 1105 52-53 0.21871279426722623 1105 54-55 0.24024666866908007 1105 56-57 0.04634563946881798 1105 58-59 0.12093541103998007 1105 60-61 0.18343365391638855 1105 62-63 0.03236551087903905 1105 64-65 0.03721122534097532 1105 66-67 0.1911752371024562 1105 68-69 0.1805462415173409 1105 70-71 0.2627596703341091 1105 72-73 0.2927748860027535 1105 74-75 0.19232449699313392 1105 76-77 0.04497091397907482 1105 78-79 0.1721270340123091 1105 80-81 0.21898384789432157 1105 82-83 0.3660716675776783 1105 84-85 0.5579884470869736 1105 86-87 0.5247773311618822 1105 88-89 0.3512809183201071 1105 90-91 0.26357216496279534 1105 92-93 -0.1399905527744636 1105 94-95 0.30809840925373777 1105 96-97 0.7736773967174138 1105 98-99 0.41452546646019783 1105 100-101 0.8893824454949915 1106 1 0.029843517192794877 1106 2 0.019902762174424993 1106 3 -0.023809593705429677 1106 4 0.13202764117062316 1106 5 0.12549903337163215 1106 6 0.011481397756867295 1106 7 -0.07181559167541707 1106 8 -0.0963477031541018 1106 9 -0.0918434099673533 1106 10-11 -0.12358551497319326 1106 12-13 0.029842333820454314 1106 14-15 0.09255754098172986 1106 16-17 -0.041931770663872214 1106 18-19 -0.06770069725816796 1106 20-21 -0.27058011945372584 1106 22-23 -0.333984708710517 1106 24-25 -0.24287941476031705 1106 26-27 -0.20592807332221952 1106 28-29 -0.5083693847615649 1106 30-31 -0.40060800762321946 1106 32-33 -0.3117089906826678 1106 34-35 -0.404679350343649 1106 36-37 -0.5654706905075741 1106 38-39 -0.36197394938323413 1106 40-41 -0.19417163537855942 1106 42-43 -0.21983451491381345 1106 44-45 -0.282112777902519 1106 46-47 -0.2801752303782976 1106 48-49 -0.6004187593879848 1106 50-51 -0.3212095962038788 1106 52-53 -0.6537242465473625 1106 54-55 -0.5184319509503226 1106 56-57 -0.6407421459245981 1106 58-59 -0.5404068805011306 1106 60-61 -0.5799554669120468 1106 62-63 -0.1960321866972734 1106 64-65 -0.20517950347519331 1106 66-67 -0.4674732479974253 1106 68-69 -0.6837227584802221 1106 70-71 -0.5904034781985708 1106 72-73 -0.46460807127943227 1106 74-75 -0.5140255904823725 1106 76-77 -0.4650619289871365 1106 78-79 -0.8447842875388751 1106 80-81 -0.3117009011107541 1106 82-83 -0.42804124292889867 1106 84-85 -0.05955752940566228 1106 86-87 -0.044971271304024896 1106 88-89 -0.05587609106673597 1106 90-91 -0.08788661418655863 1106 92-93 -0.15881303727409346 1106 94-95 -0.19863976691242158 1106 96-97 0.3001018322746809 1106 98-99 0.20815341860609138 1106 100-101 0.22985371521967934 1107 1 -0.06386501428161395 1107 2 0.07371750557653911 1107 3 0.006929125214917065 1107 4 0.03994799010016692 1107 5 -0.064768898422777 1107 6 -0.021050270907068125 1107 7 0.07172801729878131 1107 8 0.0553875598096738 1107 9 -0.015383160030687293 1107 10-11 0.029535805771864432 1107 12-13 -0.09624298486127003 1107 14-15 0.07545364046288228 1107 16-17 0.17549291822785307 1107 18-19 -0.058703790949643064 1107 20-21 0.04360085296556093 1107 22-23 0.061151357274226825 1107 24-25 0.04579008789957584 1107 26-27 -0.02036630646607307 1107 28-29 0.15193161028616942 1107 30-31 -0.02527576721044511 1107 32-33 0.07230973684803388 1107 34-35 0.16911181957574684 1107 36-37 0.1826815605893941 1107 38-39 0.17323073696213243 1107 40-41 0.07367024902999475 1107 42-43 0.1568745953972268 1107 44-45 0.15512085552457933 1107 46-47 0.25953414375623396 1107 48-49 0.25499086925090353 1107 50-51 0.20258045804401092 1107 52-53 0.4756070199498126 1107 54-55 0.4451282487154984 1107 56-57 0.15235788518295124 1107 58-59 0.34958134588305967 1107 60-61 0.38744198646928396 1107 62-63 0.32121806367253924 1107 64-65 0.10672615534195273 1107 66-67 0.14299791976937826 1107 68-69 0.1840721364694602 1107 70-71 0.39058285416915695 1107 72-73 0.5573739388456183 1107 74-75 0.6130861459881416 1107 76-77 0.4501988821979275 1107 78-79 0.5655459118513129 1107 80-81 0.6890479113553667 1107 82-83 0.5137922385043616 1107 84-85 0.9602410459474449 1107 86-87 1.001531229768986 1107 88-89 0.681853819298901 1107 90-91 0.6064686129969736 1107 92-93 0.9297857186865457 1107 94-95 0.5973432906312901 1107 96-97 0.8910684369450124 1107 98-99 0.7019548789544707 1107 100-101 0.9498871066116834 1108 1 0.14001860400259147 1108 2 0.20775275239304136 1108 3 0.11492561194821604 1108 4 0.002927100749047895 1108 5 0.2387751252188437 1108 6 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64-65 -1.0449384224992073 2316 66-67 -0.9358285924682548 2316 68-69 -1.034887196258829 2316 70-71 -1.0933850499465478 2316 72-73 -1.0942474219161547 2316 74-75 -1.249178140858021 2316 76-77 -1.2918210840927493 2316 78-79 -1.7896883967474473 2316 80-81 -1.4070250966974172 2316 82-83 -1.6034095996620827 2316 84-85 -1.2407140515070658 2316 86-87 -1.493992921375547 2316 88-89 -1.8299876604538987 2316 90-91 -1.1335489619486445 2316 92-93 -1.3321385938017514 2316 94-95 -1.6051042366314832 2316 96-97 -1.6925018700403065 2316 98-99 -1.896868175916815 2316 100-101 -2.9962874615776656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 9.0 5 22.0 6 34.0 7 76.0 8 179.0 9 398.0 10 721.0 11 1122.0 12 1716.0 13 2411.0 14 3087.0 15 4284.0 16 5708.0 17 7953.0 18 6994.0 19 4364.0 20 3681.0 21 3808.0 22 3898.0 23 4436.0 24 5077.0 25 5969.0 26 7463.0 27 9061.0 28 11158.0 29 14187.0 30 17459.0 31 21975.0 32 27876.0 33 36846.0 34 49622.0 35 68811.0 36 100276.0 37 132837.0 38 83427.0 39 4966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0339074066971 19.468681676959072 14.896091442599058 21.601319473744773 2 15.971066297452577 20.85926653217455 39.675684797533115 23.493982372839753 3 19.969595444532395 25.181169424859945 29.95738453325479 24.89185059735287 4 13.186034418491923 17.024406481193157 36.7358114606614 33.053747639653515 5 13.606576488959929 37.99720201626634 33.64207283961601 14.754148655157708 6 31.805736006270735 36.24403106530433 17.181021510407 14.769211418017939 7 28.830357238728244 33.34780347805012 20.107314274669935 17.7145250085517 8 27.245339514079248 34.40867472392958 19.08371771253089 19.262268049460285 9 27.145786963677892 16.793548872018984 19.995643617208938 36.065020547094186 10-11 25.436750383867885 26.93556081525716 26.502453533195176 21.12523526767978 12-13 26.617078930555437 24.20155589319667 27.477399052167595 21.703966124080296 14-15 23.304689561231815 25.542477865246443 26.235358543125187 24.917474030396555 16-17 22.089391747722487 28.711016893254815 26.981962427732363 22.217628931290335 18-19 21.772584126263407 26.900232701903796 30.207836393841454 21.11934677799134 20-21 22.895245867188617 25.82276578153354 30.192954011016738 21.089034340261108 22-23 22.37631477626753 25.821078450170802 30.17439336602666 21.628213407535007 24-25 22.404079046872532 25.968796641290226 30.445900321666713 21.18122399017053 26-27 22.09601983104952 26.073274255376916 30.393138898646587 21.437567014926977 28-29 21.658953304116128 26.410916414966124 30.496288535493992 21.433841745423756 30-31 21.89337403456025 26.25773694019834 30.243593775166627 21.60529525007478 32-33 21.973010931086826 26.18214284892457 30.197213650550392 21.647632569438212 34-35 22.32115464853721 26.145490747103867 30.120922712546633 21.412431891812293 36-37 22.52728120580322 25.952656331970168 30.051049678331804 21.469012783894808 38-39 22.195241674464267 26.260603457532483 30.154008988970872 21.39014587903238 40-41 22.19003507351018 26.262565834734968 30.071105732099124 21.476293359655724 42-43 22.277004073611877 26.389887072514277 29.610425869237904 21.72268298463594 44-45 22.351544362010635 26.38994881911203 29.38648128135529 21.872025537522042 46-47 22.777415171137978 26.826176091840125 28.800204934535227 21.59620380248667 48-49 22.879314999570493 27.00356796622857 28.412883947526662 21.704233086674275 50-51 22.585791278484287 27.289231059499187 28.390522405860256 21.73445525615627 52-53 22.792712848080537 27.415328237061765 28.03879956650757 21.753159348350128 54-55 22.466087954263415 27.419018356912872 28.087288524283373 22.02760516454034 56-57 22.758974075744607 27.535814992436585 27.845255299759753 21.85995563205906 58-59 22.792430422844674 27.056244011228397 28.009921126583354 22.141404439343574 60-61 22.608628277681504 27.185741102572898 28.48856699891297 21.717063620832633 62-63 22.48573043817776 27.20847406011894 28.617672394946798 21.688123106756496 64-65 22.39319596280701 27.406317530429686 28.248997567466184 21.95148893929712 66-67 22.29761704797427 27.54979088098432 27.934664976227236 22.217927094814172 68-69 22.420263455601788 27.362414434452475 28.12271585527199 22.09460625467375 70-71 22.64068892205603 27.39996042440986 27.938679310403014 22.020671343131106 72-73 22.51559631057328 27.33085653541785 27.968514396771365 22.1850327572375 74-75 22.55405312123699 27.764704980020095 27.490355189789923 22.19088670895299 76-77 22.644493463701377 27.64496899821751 27.619658290100595 22.090879247980514 78-79 22.653120709360213 27.52107447208352 27.550528875286684 22.275275943269595 80-81 22.72999946302135 27.76325378378171 27.325002493115168 22.181744260081775 82-83 22.808213976401966 27.795161489525412 27.330595750328136 22.066028783744496 84-85 22.528875846327946 27.679996931162133 27.31112261925312 22.480004603256802 86-87 22.558628132759267 27.595818922237996 27.381963253336895 22.46358969166584 88-89 22.48354307804099 27.586857788714532 27.24624803664068 22.6833510966038 90-91 22.309205760660046 27.719109504106864 27.50463480214613 22.46704993308696 92-93 22.420854124853463 27.603159697287744 27.485699116782364 22.490287061076433 94-95 22.207790015743296 27.554909729233735 27.49698483672085 22.740315418302117 96-97 22.33214745055667 27.51851726794709 27.476387221934022 22.672948059562216 98-99 22.11035801661924 27.739150988283036 27.077174790571174 23.07331620452655 100-101 21.784020912095215 28.02035030522388 27.005444451095872 23.19018433158503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 168.0 1 129.5 2 135.0 3 178.5 4 159.0 5 135.0 6 196.5 7 421.5 8 679.0 9 827.5 10 833.0 11 792.5 12 813.5 13 891.5 14 999.0 15 1068.0 16 1108.0 17 1195.5 18 1296.5 19 1371.0 20 1463.0 21 1610.0 22 1818.5 23 2054.5 24 2319.0 25 2782.0 26 3464.5 27 4181.0 28 4856.0 29 5902.5 30 7355.5 31 8698.5 32 10216.0 33 11754.0 34 13233.0 35 14549.5 36 16484.0 37 18067.0 38 19016.0 39 19803.5 40 19973.0 41 20324.5 42 20455.0 43 21023.5 44 22037.0 45 22866.0 46 23194.5 47 24060.5 48 26896.0 49 26895.0 50 24222.0 51 22722.0 52 21815.0 53 21614.5 54 21137.0 55 19588.0 56 17516.5 57 15616.5 58 14496.5 59 13165.5 60 10922.5 61 9013.0 62 7347.5 63 5573.0 64 4003.0 65 2867.5 66 2138.0 67 1758.5 68 1370.0 69 1030.0 70 827.0 71 609.5 72 480.5 73 361.0 74 270.0 75 247.5 76 192.5 77 110.5 78 61.0 79 47.5 80 37.0 81 25.5 82 21.0 83 17.5 84 12.5 85 5.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021781913955304124 2 0.012578288340386889 3 0.006289144170193444 4 0.0 5 0.0029144814447237917 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0013038469621132751 14-15 1.533937602486206E-4 16-17 0.0 18-19 0.0015339376024862063 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0015339376024862063 28-29 0.0027610876844751707 30-31 0.0018407251229834473 32-33 0.009280322495041547 34-35 0.001994118883232068 36-37 4.601812807458618E-4 38-39 0.0010737563217403444 40-41 7.66968801243103E-5 42-43 0.005905659769571893 44-45 0.002070815763356378 46-47 2.300906403729309E-4 48-49 7.669688012431031E-4 50-51 7.66968801243103E-5 52-53 3.834844006215516E-4 54-55 0.0015339376024862063 56-57 0.014572407223618957 58-59 0.027073998683881533 60-61 0.021705217075179816 62-63 0.014188922822997406 64-65 0.019557704431699127 66-67 0.0023776032838536197 68-69 6.902719211187927E-4 70-71 0.0 72-73 0.0 74-75 0.0018407251229834473 76-77 0.0029144814447237917 78-79 0.009510413135414479 80-81 0.018714038750331716 82-83 0.019250916911201888 84-85 0.031369023970842916 86-87 0.011581228898770855 88-89 0.04471428111247291 90-91 0.049852972080801694 92-93 0.03205929589196171 94-95 0.032366083412458946 96-97 0.055681934970249286 98-99 0.05874981017522169 100-101 0.06565252938640961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 651917.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4313631187588 #Duplication Level Percentage of deduplicated Percentage of total 1 85.13126256929192 56.55385816499031 2 9.011131632028059 11.972435151163792 3 2.420539361100675 4.82399187721582 4 1.0612022362533569 2.819884443959425 5 0.5567921204459324 1.8494229767503712 6 0.3546632295737559 1.4136457073211537 7 0.24747885348849344 1.1508250306215753 8 0.193961210694368 1.0308086094873314 9 0.14018558396147018 0.8381447488943709 >10 0.7944764285267093 9.791448751135299 >50 0.05617428832087613 2.5219133988482354 >100 0.030745472516682765 3.5675543631221704 >500 4.6233793256441827E-4 0.21192479966416536 >1k 9.246758651288365E-4 1.4541419768260737 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3284 0.50374510865647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2962 0.4543523178564142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1849 0.2836250626996995 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1374 0.21076302658160473 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.13452632773804027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.533937602486206E-4 0.0 9 0.0 0.0 0.0 3.067875204972412E-4 0.0 10-11 0.0 0.0 0.0 3.067875204972412E-4 0.0 12-13 0.0 0.0 0.0 4.601812807458618E-4 0.0 14-15 0.0 0.0 0.0 6.135750409944824E-4 0.0 16-17 0.0 0.0 0.0 6.902719211187927E-4 0.0 18-19 0.0 0.0 0.0 7.66968801243103E-4 0.0 20-21 1.533937602486206E-4 0.0 0.0 8.436656813674134E-4 0.0 22-23 1.533937602486206E-4 0.0 0.0 9.203625614917236E-4 0.0 24-25 1.533937602486206E-4 0.0 0.0 0.0013805438422375854 0.0 26-27 1.533937602486206E-4 0.0 0.0 0.0017640282428591368 0.0 28-29 1.533937602486206E-4 0.0 0.0 0.003297965845345343 7.66968801243103E-5 30-31 1.533937602486206E-4 0.0 0.0 0.006519234810566376 1.533937602486206E-4 32-33 1.533937602486206E-4 0.0 0.0 0.010737563217403441 1.533937602486206E-4 34-35 1.533937602486206E-4 0.0 0.0 0.01533937602486206 1.533937602486206E-4 36-37 1.533937602486206E-4 0.0 0.0 0.022318792116174296 1.533937602486206E-4 38-39 1.533937602486206E-4 0.0 0.0 0.031215630210594293 1.533937602486206E-4 40-41 1.533937602486206E-4 0.0 0.0 0.041876496547873426 1.533937602486206E-4 42-43 1.533937602486206E-4 0.0 0.0 0.05851971953484876 1.533937602486206E-4 44-45 1.533937602486206E-4 0.0 0.0 0.07125140163548427 1.533937602486206E-4 46-47 1.533937602486206E-4 0.0 0.0 0.09548761575476633 1.533937602486206E-4 48-49 1.533937602486206E-4 0.0 0.0 0.1229450988392694 1.533937602486206E-4 50-51 1.533937602486206E-4 0.0 0.0 0.13414284333741872 1.533937602486206E-4 52-53 1.533937602486206E-4 0.0 0.0 0.15454421345048525 1.533937602486206E-4 54-55 1.533937602486206E-4 0.0 0.0 0.1859899343014525 1.533937602486206E-4 56-57 1.533937602486206E-4 0.0 0.0 0.2381438127859835 1.533937602486206E-4 58-59 1.533937602486206E-4 0.0 0.0 0.3001915888065505 1.533937602486206E-4 60-61 1.533937602486206E-4 0.0 0.0 0.3735138062053912 1.533937602486206E-4 62-63 1.533937602486206E-4 0.0 0.0 0.45128444265144185 1.533937602486206E-4 64-65 1.533937602486206E-4 0.0 0.0 0.537338342150918 2.300906403729309E-4 66-67 1.533937602486206E-4 0.0 0.0 0.6529205404982537 3.067875204972412E-4 68-69 1.533937602486206E-4 0.0 0.0 0.7887507151984071 3.067875204972412E-4 70-71 1.533937602486206E-4 0.0 0.0 0.8674417142059496 3.067875204972412E-4 72-73 1.533937602486206E-4 0.0 0.0 0.9761978902222215 3.067875204972412E-4 74-75 1.533937602486206E-4 0.0 0.0 1.088252032083839 3.067875204972412E-4 76-77 1.533937602486206E-4 0.0 0.0 1.2061351368349038 3.067875204972412E-4 78-79 1.533937602486206E-4 0.0 0.0 1.3369800143269772 3.067875204972412E-4 80-81 1.533937602486206E-4 0.0 0.0 1.553955488198651 3.067875204972412E-4 82-83 1.533937602486206E-4 0.0 0.0 1.7855800661740682 3.067875204972412E-4 84-85 1.533937602486206E-4 0.0 0.0 2.15180766876765 3.067875204972412E-4 86-87 1.533937602486206E-4 0.0 0.0 2.649953905175045 3.067875204972412E-4 88-89 1.533937602486206E-4 0.0 0.0 3.1564601015159903 3.067875204972412E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 50 0.0016164375 37.99457 2 TACCTGG 615 0.0 32.43439 2 GCACCGT 125 2.155648E-7 30.393326 6 GTACCTG 680 0.0 29.340796 1 TAGACTG 295 0.0 28.976686 5 GTGTAGG 260 0.0 27.406237 1 GGTATCA 4035 0.0 26.018375 1 GTATCAA 5575 0.0 25.733185 1 ACCGTGG 190 1.1918019E-8 24.994513 8 GAGTACT 3525 0.0 22.635063 12-13 GTACATG 3550 0.0 22.480833 1 ACGAATC 85 3.1534346E-6 22.349747 72-73 GCCGCGC 150 3.0357589E-5 22.161798 8 AGTACTT 3650 0.0 21.92495 12-13 ACATGGG 3450 0.0 21.611197 3 TACATGG 3550 0.0 21.539175 2 GTACTTT 3710 0.0 21.442352 14-15 TCAACGC 6595 0.0 21.386507 4 ATCAACG 6620 0.0 21.305744 3 ACCTGGG 915 0.0 21.27948 3 >>END_MODULE