##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936523_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645253 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55064757544715 31.0 31.0 34.0 30.0 34.0 2 31.610242803985415 33.0 31.0 34.0 30.0 34.0 3 31.6233492908983 33.0 31.0 34.0 30.0 34.0 4 35.23965173350608 37.0 35.0 37.0 33.0 37.0 5 35.26883718479418 37.0 35.0 37.0 33.0 37.0 6 35.278069222460026 37.0 35.0 37.0 33.0 37.0 7 35.20444848764748 37.0 35.0 37.0 32.0 37.0 8 35.19099330030236 37.0 35.0 37.0 32.0 37.0 9 36.69350626808399 39.0 37.0 39.0 32.0 39.0 10-11 36.56175407165871 39.0 37.0 39.0 32.0 39.0 12-13 36.48472924573772 39.0 37.0 39.0 32.0 39.0 14-15 37.54877234201158 40.0 37.0 41.0 32.0 41.0 16-17 37.4799171797729 40.0 37.0 41.0 32.0 41.0 18-19 37.50354667084074 40.0 37.0 41.0 32.0 41.0 20-21 37.39425000736145 40.0 37.0 41.0 32.0 41.0 22-23 37.50450056024536 40.0 37.0 41.0 32.0 41.0 24-25 37.53359534942108 40.0 37.0 41.0 32.0 41.0 26-27 37.41056686292043 40.0 37.0 41.0 32.0 41.0 28-29 37.2773237784249 39.5 37.0 41.0 31.5 41.0 30-31 37.053031911513784 39.0 36.0 41.0 31.0 41.0 32-33 36.8252197200168 39.0 35.5 41.0 30.5 41.0 34-35 36.592392441414454 39.0 35.0 41.0 30.0 41.0 36-37 36.345263795751436 39.0 35.0 40.0 30.0 41.0 38-39 36.111267983256184 39.0 35.0 40.0 30.0 41.0 40-41 35.902191853428036 38.0 35.0 40.0 29.0 41.0 42-43 35.6415375054436 38.0 35.0 40.0 28.0 41.0 44-45 35.320730783119174 38.0 35.0 40.0 26.5 41.0 46-47 34.98034801852916 38.0 34.5 40.0 25.0 41.0 48-49 34.73944638769599 38.0 34.0 40.0 24.0 41.0 50-51 33.76173764399391 37.5 33.0 39.5 22.5 40.5 52-53 33.88511250625724 38.0 33.5 39.5 23.0 40.5 54-55 34.310105493504096 38.0 34.0 40.0 23.0 41.0 56-57 34.164488193003365 38.0 34.0 40.0 22.5 41.0 58-59 33.92858227702932 38.0 34.0 40.0 21.5 41.0 60-61 33.5933812008623 38.0 33.0 40.0 19.5 41.0 62-63 33.247218532885555 37.0 33.0 40.0 18.0 41.0 64-65 32.88025704646084 36.5 32.0 39.5 16.5 41.0 66-67 32.38007649712593 36.0 32.0 39.0 12.5 41.0 68-69 31.98256575327817 35.5 31.0 39.0 8.0 40.5 70-71 31.53856859247458 35.0 31.0 38.5 7.5 40.0 72-73 31.127722769208358 35.0 31.0 37.5 4.5 40.0 74-75 30.69110178488128 35.0 30.0 37.0 2.0 39.0 76-77 30.203051361249 34.5 30.0 36.5 2.0 39.0 78-79 29.66636807577803 34.0 29.0 36.0 2.0 39.0 80-81 29.141993140675055 34.0 29.0 35.5 2.0 37.5 82-83 28.695060697121903 34.0 28.5 35.0 2.0 37.0 84-85 28.288840191366795 34.0 27.5 35.0 2.0 36.5 86-87 27.935617501972096 34.0 27.0 35.0 2.0 36.0 88-89 27.466701433391243 34.0 26.0 35.0 2.0 36.0 90-91 26.99966292291551 33.0 25.5 35.0 2.0 35.5 92-93 26.5038612761196 33.0 25.0 35.0 2.0 35.0 94-95 25.992821420435085 33.0 23.5 35.0 2.0 35.0 96-97 25.379065265872455 32.5 19.5 35.0 2.0 35.0 98-99 24.608499301824246 32.0 9.0 35.0 2.0 35.0 100-101 21.752828735395262 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47334875369376306 1101 2 0.5451977573244449 1101 3 0.4581572512430654 1101 4 0.43089022537532173 1101 5 0.3741301107196833 1101 6 0.5187380496189178 1101 7 0.42224297094292496 1101 8 0.4051710259063981 1101 9 0.4942483299882383 1101 10-11 0.6462240934284083 1101 12-13 0.717963711251052 1101 14-15 0.9269181610586372 1101 16-17 0.9592733817484174 1101 18-19 0.7413587457495225 1101 20-21 0.7823212636383943 1101 22-23 0.797877888833689 1101 24-25 0.7776860897777951 1101 26-27 0.700143810121979 1101 28-29 0.5895588455726539 1101 30-31 0.7871792028237508 1101 32-33 0.6732060467161816 1101 34-35 0.7503710725793482 1101 36-37 0.4513559192591501 1101 38-39 0.3188476403195821 1101 40-41 0.5997502379927369 1101 42-43 0.6409552997014529 1101 44-45 0.777363431106302 1101 46-47 0.9548804940653355 1101 48-49 1.1348727998122925 1101 50-51 0.8759923919350356 1101 52-53 1.1274049526702257 1101 54-55 1.0607817688707328 1101 56-57 0.9412391012697938 1101 58-59 0.8271720099106403 1101 60-61 0.7930511517384957 1101 62-63 0.9653502049473417 1101 64-65 1.161448031920692 1101 66-67 1.060038549529068 1101 68-69 0.8135812235811812 1101 70-71 1.0871830022503808 1101 72-73 1.034110725733754 1101 74-75 0.9516636835311125 1101 76-77 0.9879632035117574 1101 78-79 0.7226128345267604 1101 80-81 1.160166267666174 1101 82-83 1.2168650672317938 1101 84-85 0.9012656867672604 1101 86-87 1.1055902078901347 1101 88-89 1.0078155299531026 1101 90-91 0.9147924989568139 1101 92-93 0.8719643104408199 1101 94-95 1.0171429979055162 1101 96-97 1.09896021712078 1101 98-99 1.0654511159990605 1101 100-101 1.0810259706035303 1102 1 0.22453867752820855 1102 2 0.2831799175901111 1102 3 0.1291413560502015 1102 4 0.22040012793971897 1102 5 0.2603848612918398 1102 6 0.2530970025902093 1102 7 0.27426492914310785 1102 8 0.42090200146147794 1102 9 0.45392646406254755 1102 10-11 0.381679131771854 1102 12-13 0.4951273394630604 1102 14-15 0.10507569498384584 1102 16-17 0.1837245570751591 1102 18-19 0.2073566395973785 1102 20-21 0.11668725449435868 1102 22-23 0.06610601013553463 1102 24-25 0.09920012123453859 1102 26-27 -0.0843079386620289 1102 28-29 -0.18957930028359726 1102 30-31 0.04292159539174634 1102 32-33 -0.02052867195089192 1102 34-35 0.02541812214170136 1102 36-37 0.014509204387316288 1102 38-39 -0.2388252179478414 1102 40-41 0.05573739254989363 1102 42-43 -0.2077028102876497 1102 44-45 -0.020193119600349974 1102 46-47 0.10553423535269957 1102 48-49 -0.023149867389598455 1102 50-51 0.20670910925709762 1102 52-53 0.24461049539539914 1102 54-55 0.12919652444760032 1102 56-57 0.13114599266219784 1102 58-59 0.28098524009117654 1102 60-61 0.4068902396286376 1102 62-63 0.2136037668989701 1102 64-65 0.023487245971438142 1102 66-67 0.15689417905482372 1102 68-69 0.2631612964623855 1102 70-71 0.26371139620509254 1102 72-73 0.1420849097301229 1102 74-75 0.13580602605192738 1102 76-77 0.23016548795698455 1102 78-79 0.173146931774685 1102 80-81 0.31166976836214744 1102 82-83 0.44232891596419677 1102 84-85 0.37217967339006464 1102 86-87 0.33665178156114806 1102 88-89 0.28444610356676847 1102 90-91 0.16298610855753282 1102 92-93 0.18085967534210212 1102 94-95 -0.0839145094321303 1102 96-97 -0.03793576294376422 1102 98-99 0.2825763747064869 1102 100-101 0.17975967268726833 1103 1 0.10106237955981356 1103 2 0.1512220912398412 1103 3 0.04738724054064747 1103 4 0.4488618358073566 1103 5 0.2766789757002499 1103 6 0.39230308088124133 1103 7 0.5948644996986303 1103 8 0.2752239747037848 1103 9 0.1543311328947894 1103 10-11 0.31773595057931203 1103 12-13 -0.015272924810460609 1103 14-15 0.3058822797991354 1103 16-17 0.45977752189374854 1103 18-19 0.386894711501796 1103 20-21 0.4996127073380521 1103 22-23 0.4053836625057343 1103 24-25 0.3238064637990874 1103 26-27 0.36112646094155565 1103 28-29 0.5603243499944384 1103 30-31 0.25620133992734395 1103 32-33 0.41636776586224045 1103 34-35 0.7081260998986778 1103 36-37 0.4752659626320934 1103 38-39 0.45723270199929544 1103 40-41 0.49083484341154104 1103 42-43 0.7966205393792549 1103 44-45 0.44073018602096425 1103 46-47 0.6684753794368206 1103 48-49 0.37837108179279966 1103 50-51 0.5432063839363863 1103 52-53 0.5385129344197921 1103 54-55 0.7903359287310394 1103 56-57 0.6364314631241257 1103 58-59 0.43414688739613183 1103 60-61 0.7635617696622248 1103 62-63 0.9203000725753441 1103 64-65 0.8528339948565318 1103 66-67 1.008669766788124 1103 68-69 0.8885150375844475 1103 70-71 0.8665371959077923 1103 72-73 0.538708540187649 1103 74-75 0.671364579154389 1103 76-77 0.5748414776062738 1103 78-79 0.39712700114648314 1103 80-81 0.5949420802049019 1103 82-83 0.3597916148575493 1103 84-85 0.4060479770843912 1103 86-87 0.6129593849306367 1103 88-89 0.3607206727458703 1103 90-91 0.29635477012224953 1103 92-93 0.44932165849467154 1103 94-95 0.5549295691892411 1103 96-97 0.5283707392860428 1103 98-99 0.18653910112833216 1103 100-101 0.02809970462032041 1104 1 0.1655868011536441 1104 2 0.19359151879015712 1104 3 0.10498959805522645 1104 4 0.11851824059763061 1104 5 0.08341607520492289 1104 6 0.2199913371982518 1104 7 0.23442497370954385 1104 8 0.1656779216597073 1104 9 0.2101747594544463 1104 10-11 0.1863295928139408 1104 12-13 0.2628291505547651 1104 14-15 -0.08481619659436745 1104 16-17 -0.037642379040484286 1104 18-19 -0.09353352851325525 1104 20-21 -0.03656354195657485 1104 22-23 -0.0063129955919478675 1104 24-25 0.0400378889668076 1104 26-27 -0.250747324248735 1104 28-29 -0.045669756219119506 1104 30-31 0.08861089538536504 1104 32-33 -0.18404918817876137 1104 34-35 0.12383711626333849 1104 36-37 -0.31959108169670714 1104 38-39 -0.25707540509205984 1104 40-41 -0.17135557605049456 1104 42-43 -0.11815938412691906 1104 44-45 -0.29745089116255485 1104 46-47 -0.4732795895267472 1104 48-49 0.04070257593036786 1104 50-51 -0.20802566822988666 1104 52-53 -0.2695283220853213 1104 54-55 -0.47586101151599536 1104 56-57 -0.4037904943653672 1104 58-59 -0.050907034479841684 1104 60-61 -0.13418573801850187 1104 62-63 0.026157806602554956 1104 64-65 0.23500372336616238 1104 66-67 0.05222108662008651 1104 68-69 -0.32053568659888754 1104 70-71 -0.3321978345016454 1104 72-73 -0.46013934996220485 1104 74-75 -0.7368459669613507 1104 76-77 -0.667308500448776 1104 78-79 -0.36705570623328754 1104 80-81 -0.48523379289058965 1104 82-83 -0.6652021151568732 1104 84-85 -0.533067327950409 1104 86-87 -0.5885877340109893 1104 88-89 -0.629866511403609 1104 90-91 -0.5895236548573699 1104 92-93 -0.9153077853576086 1104 94-95 -0.76966601674091 1104 96-97 -0.6010715454966977 1104 98-99 -0.8425708744187226 1104 100-101 -0.9074103587065352 1105 1 0.13695314678998116 1105 2 -0.17597667814707307 1105 3 0.17137468067512884 1105 4 0.36794743953726083 1105 5 0.22994898109210737 1105 6 0.24108674036933309 1105 7 0.20231636940588515 1105 8 0.31109095430986144 1105 9 0.525739994577684 1105 10-11 0.30292260413635574 1105 12-13 0.1441342875971614 1105 14-15 0.2566181103043803 1105 16-17 0.29166721075161917 1105 18-19 0.3181928814405808 1105 20-21 0.11641866375912713 1105 22-23 -0.1169180280014146 1105 24-25 -0.15739576272252975 1105 26-27 0.023801299978636337 1105 28-29 -0.28737935135987414 1105 30-31 -0.5350908038133468 1105 32-33 -0.11814966253700732 1105 34-35 -0.34888728460447993 1105 36-37 -0.15726044163064756 1105 38-39 -0.02269847247499257 1105 40-41 -0.19917356154897448 1105 42-43 -0.03798648907073954 1105 44-45 0.028443085256583345 1105 46-47 -0.2505150737967128 1105 48-49 0.07533340219624307 1105 50-51 0.06240616826193701 1105 52-53 0.09506563193635742 1105 54-55 0.09483892597167198 1105 56-57 -0.1085969234406221 1105 58-59 -0.08678225720090182 1105 60-61 -0.0828629504367342 1105 62-63 -0.13867499799460603 1105 64-65 -0.18064391436989524 1105 66-67 0.3232721962974665 1105 68-69 -0.08133494179961076 1105 70-71 0.12884729137545037 1105 72-73 0.017367076773012968 1105 74-75 0.12626958724218085 1105 76-77 0.035209710027221064 1105 78-79 0.18543459098441062 1105 80-81 0.1027531430359474 1105 82-83 -0.03887707111409355 1105 84-85 0.19991394175183785 1105 86-87 -0.2245069159352333 1105 88-89 -0.31404303685481594 1105 90-91 -0.3873372277210052 1105 92-93 -0.46685789049197624 1105 94-95 -0.564158413303069 1105 96-97 -0.1872157049399057 1105 98-99 0.06885300261585314 1105 100-101 0.11494197681417262 1106 1 0.35447311976300355 1106 2 0.2679961363387804 1106 3 0.39211448752284284 1106 4 0.2909394314744631 1106 5 0.2104695523578286 1106 6 0.5549035269147282 1106 7 0.7463512779916712 1106 8 0.45727820671199737 1106 9 0.5481202935730423 1106 10-11 0.6934642949454854 1106 12-13 0.6910511928574223 1106 14-15 0.676771514816302 1106 16-17 0.6358421613500127 1106 18-19 0.6464224823963391 1106 20-21 0.3402716687172145 1106 22-23 0.4363706000964527 1106 24-25 0.5896981767800824 1106 26-27 0.4084015857247394 1106 28-29 0.589413875287363 1106 30-31 0.6831491964886425 1106 32-33 0.5622384161507554 1106 34-35 0.3844836700781542 1106 36-37 0.438523954065424 1106 38-39 0.250986463194387 1106 40-41 0.44243401506363256 1106 42-43 0.47265066433943304 1106 44-45 0.4821829808101583 1106 46-47 0.35275269898795614 1106 48-49 0.35584019574373826 1106 50-51 0.16233803461428664 1106 52-53 0.16551035025751304 1106 54-55 0.4602419431032274 1106 56-57 0.5982000213031782 1106 58-59 0.4887904146006363 1106 60-61 0.5404768817015935 1106 62-63 0.3357333622493144 1106 64-65 0.38258637547177443 1106 66-67 0.2917806733688906 1106 68-69 0.38106698632599034 1106 70-71 0.37517024486527006 1106 72-73 0.31489973279623484 1106 74-75 0.5970504441407591 1106 76-77 0.20780016640941668 1106 78-79 0.326701499358812 1106 80-81 0.4851603180186288 1106 82-83 0.3958061640451298 1106 84-85 0.1737494886423221 1106 86-87 0.23515928581208456 1106 88-89 0.2481812908208454 1106 90-91 0.264667807751902 1106 92-93 0.5529810800671129 1106 94-95 0.6211009380872561 1106 96-97 0.4764494323371302 1106 98-99 0.39509427122285246 1106 100-101 0.20246162893622355 1107 1 0.2421338081312392 1107 2 0.2309622189982612 1107 3 0.18572928467537508 1107 4 0.3046908137399953 1107 5 0.392358418208957 1107 6 0.35351823767566515 1107 7 0.4754466715917758 1107 8 0.5246722906155199 1107 9 0.5306087170992058 1107 10-11 0.4319418158522481 1107 12-13 0.34884220294795654 1107 14-15 0.12867191394547461 1107 16-17 0.36585330586587617 1107 18-19 0.3628928241614915 1107 20-21 0.36244299189089446 1107 22-23 0.306127710124791 1107 24-25 0.16138268331128103 1107 26-27 0.0964015770855795 1107 28-29 0.3094532698550623 1107 30-31 0.47519814596682863 1107 32-33 0.11129372405039817 1107 34-35 0.29105655866754887 1107 36-37 0.307677402817923 1107 38-39 0.6115590666915196 1107 40-41 0.7504276134463836 1107 42-43 0.6845379319460392 1107 44-45 0.5514118062300213 1107 46-47 0.636579328333454 1107 48-49 0.8572364999112736 1107 50-51 0.5206417788260538 1107 52-53 0.6248188287287348 1107 54-55 0.7719125837832976 1107 56-57 0.6932512947152958 1107 58-59 0.6699373926226144 1107 60-61 0.7295751262243613 1107 62-63 0.9504829994046133 1107 64-65 0.9052911667496772 1107 66-67 0.6089274613293583 1107 68-69 0.9528951188852659 1107 70-71 0.7509920449205616 1107 72-73 0.7643908862968232 1107 74-75 0.9822349333936451 1107 76-77 0.8639529758641125 1107 78-79 0.7357376283241841 1107 80-81 0.7564439675452057 1107 82-83 0.6747052327437295 1107 84-85 0.6152850560623229 1107 86-87 0.9334333977064766 1107 88-89 0.6461808934195048 1107 90-91 0.7999479756971617 1107 92-93 0.8323682612821237 1107 94-95 0.8578549466573051 1107 96-97 1.1627421097854658 1107 98-99 1.4363322195952328 1107 100-101 1.0686194491034762 1108 1 0.18365623621510352 1108 2 0.13234670624025568 1108 3 0.35084183140055103 1108 4 0.10481372126478306 1108 5 0.1862394518510868 1108 6 0.19793864235631276 1108 7 0.3053822721118493 1108 8 0.47321365255278636 1108 9 0.34604427698868534 1108 10-11 0.38840106337378444 1108 12-13 0.17711448882476333 1108 14-15 0.4416518627509376 1108 16-17 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-4.125318986814605 2316 94-95 -3.84238482793592 2316 96-97 -4.21702072795151 2316 98-99 -4.969896788465672 2316 100-101 -5.848205823325241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1538.0 3 514.0 4 550.0 5 598.0 6 658.0 7 770.0 8 955.0 9 1222.0 10 1622.0 11 2151.0 12 2929.0 13 3696.0 14 4141.0 15 5238.0 16 6585.0 17 8715.0 18 6740.0 19 4755.0 20 4705.0 21 4824.0 22 5472.0 23 6010.0 24 6790.0 25 7652.0 26 8981.0 27 10165.0 28 12245.0 29 14595.0 30 17585.0 31 21575.0 32 27134.0 33 34480.0 34 46037.0 35 63110.0 36 86557.0 37 111289.0 38 89086.0 39 13573.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.875022281467245 18.91157938789903 14.19488340011315 21.018514930520574 2 14.93847386669932 21.650782038306005 40.01050880926511 23.400235285729565 3 20.679879220763116 26.50989546519847 30.29633694183004 22.513888372208374 4 11.83715923059395 16.533177496934684 39.21372889736964 32.41593437510172 5 12.220153447610308 39.29905230830288 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28.04324359509848 30.006579064625942 21.094387492183 52-53 21.32160522479951 28.505162108734783 28.799940486384322 21.373292180081382 54-55 20.479271429313158 27.64780687173216 29.44149061892396 22.43143108003072 56-57 21.550800274313346 27.949352767736414 28.538351562772423 21.961495395177817 58-59 22.36427716781461 27.420134777406496 28.481026391325642 21.734561663453253 60-61 21.35042690005006 27.551132691601328 29.973513443980703 21.12492696436791 62-63 21.260502703988607 27.738296986984125 29.739380287190787 21.26182002183648 64-65 20.83468293733756 28.752299492755533 29.11479408788208 21.298223482024824 66-67 20.966301887962302 28.091577022041726 28.537371733267197 22.40474935672877 68-69 20.59993410725014 28.330781604294657 29.089478478265086 21.97980581019012 70-71 21.38683822469077 28.207990845276804 28.424376621342724 21.9807943086897 72-73 21.196430965627933 27.497033683866263 29.319060915849622 21.987474434656182 74-75 20.58460357877847 29.218009386832854 28.66138802655598 21.535999007832697 76-77 21.166019592812404 27.893830798648544 28.69225456859461 22.24789503994444 78-79 21.153656794934317 27.928703954249457 29.02408710963213 21.8935521411841 80-81 21.53349939819583 28.18901924532516 28.412834559690285 21.864646796788723 82-83 21.12944890484918 28.041396483981305 29.08902306282503 21.740131548344483 84-85 20.906163414775918 28.25141927108562 28.694658115518013 22.14775919862045 86-87 21.010688800205234 28.561983957886543 28.572137194250015 21.85519004765821 88-89 21.097507194436975 28.183281624326767 27.982620193406927 22.73659098782933 90-91 20.79500035658583 27.566642790919776 28.696787316707134 22.941569535787266 92-93 21.214021353692328 28.240122464006824 28.76082701959037 21.785029162710483 94-95 20.74556179083009 27.847316459600613 28.82032411888751 22.586797630681787 96-97 19.97712099675571 27.851543790195198 29.42881545355764 22.742519759491454 98-99 20.421794308211282 28.408704140025414 28.133007397138986 23.036494154624318 100-101 20.448359556137994 27.820159895780826 28.575128529222464 23.156352018858705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 185.0 1 159.5 2 218.0 3 302.5 4 266.0 5 262.0 6 410.5 7 760.0 8 1223.0 9 1462.5 10 1304.0 11 1084.5 12 1096.0 13 1185.5 14 1262.0 15 1325.0 16 1342.5 17 1385.0 18 1493.0 19 1577.0 20 1690.5 21 1924.5 22 2222.5 23 2609.0 24 3008.5 25 3606.5 26 4537.0 27 5563.5 28 6437.0 29 7562.0 30 9022.0 31 10482.0 32 12483.5 33 13955.5 34 15128.0 35 16243.5 36 17605.0 37 19035.0 38 19186.0 39 19066.0 40 19197.0 41 19703.5 42 19960.5 43 20200.5 44 21098.0 45 21878.0 46 22393.5 47 26414.5 48 37975.5 49 37548.0 50 25557.5 51 19992.0 52 17317.5 53 16132.0 54 15403.5 55 14259.0 56 12768.5 57 11465.0 58 10636.5 59 9477.5 60 7919.5 61 6669.5 62 5554.0 63 4281.0 64 3014.0 65 2204.5 66 1681.5 67 1241.5 68 865.0 69 608.5 70 475.0 71 372.0 72 298.0 73 292.5 74 236.0 75 169.0 76 133.0 77 83.0 78 62.0 79 44.5 80 26.0 81 19.5 82 13.0 83 8.5 84 4.5 85 3.5 86 2.0 87 2.0 88 2.0 89 1.0 90 1.0 91 1.0 92 2.0 93 3.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01518784104839497 2 0.012398237590526507 3 0.016892598717092363 4 0.020457092024368736 5 0.03285532961489524 6 0.048198148633171796 7 0.005579206915736928 8 0.02340167345211878 9 0.03440510931371105 10-11 0.04269643070237566 12-13 0.0340176643890071 14-15 0.027431100669039896 16-17 0.03192546179560576 18-19 0.02433154127140827 20-21 0.05284748772961923 22-23 0.013328105409815993 24-25 0.0029445814277500454 26-27 0.0014722907138750227 28-29 0.0 30-31 0.003099559397631627 32-33 0.002092202593401348 34-35 7.748898494079066E-5 36-37 1.5497796988158131E-4 38-39 0.0 40-41 0.0020147136084605576 42-43 0.004261894171743486 44-45 0.00232466954822372 46-47 0.0012398237590526505 48-49 5.424228945855346E-4 50-51 0.0037969602620987427 52-53 0.00348700432233558 54-55 0.0068965196597303696 56-57 8.523788343486973E-4 58-59 0.006354096765144834 60-61 0.003099559397631627 62-63 3.8744492470395335E-4 64-65 3.0995593976316263E-4 66-67 0.01751251059661869 68-69 0.04114665100355984 70-71 0.052692509759737646 72-73 0.014335462214046274 74-75 0.03107308296125706 76-77 0.026268765894928034 78-79 0.0564894700218364 80-81 0.0813634341878302 82-83 0.04943797239222445 84-85 0.017899955521322643 86-87 0.021464448828599013 88-89 0.021154492888835853 90-91 0.03827955856075059 92-93 0.02564885401540171 94-95 0.013715550334519948 96-97 0.01859735638578976 98-99 0.05292497671456003 100-101 0.07097991020576425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 645253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.16452107513608 #Duplication Level Percentage of deduplicated Percentage of total 1 84.4631050814504 52.50608475907257 2 9.585720986047045 11.917835085149914 3 2.4857982135642964 4.6358536630696 4 1.0447214131613374 2.597784252244556 5 0.5648726353826821 1.75575184235072 6 0.3509208561697601 1.3088896175441909 7 0.24257164017579394 1.055554488655693 8 0.17783856895882047 0.8844199574410109 9 0.13961426473977873 0.7811148512525042 >10 0.8484126122311444 9.756984290658684 >50 0.0631137418150726 2.7219604300981963 >100 0.025796455558658404 2.974956623732595 >500 0.003506314347757803 1.5064726968358806 >1k 0.0037567653725976455 4.747817785242888 >5k 2.504510248398431E-4 0.848519656651049 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5450 0.8446299358546183 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3900 0.6044140825381672 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3786 0.586746593971667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 0.49128016452461276 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2484 0.384965277185848 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2350 0.36419822922171613 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2000 0.30995593976316266 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1951 0.30236201923896516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1839 0.28500448661222805 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1512 0.23432669046095098 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1461 0.22642281399699032 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1333 0.2065856338521479 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1306 0.2024012286653452 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1200 0.18597356385789762 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1189 0.1842688061892002 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1014 0.15714766145992348 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 966 0.14970871890560758 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 820 0.12708193530289671 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 809 0.12537717763419928 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 719 0.11142916034485699 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 714 0.11065427049544907 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 706 0.10941444673639641 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 699 0.10832960094722535 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 694 0.10755471109781745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5497796988158134E-4 0.0 0.0 0.0 0.0 7 1.5497796988158134E-4 0.0 0.0 0.0 0.0 8 1.5497796988158134E-4 0.0 0.0 0.0 0.0 9 1.5497796988158134E-4 0.0 0.0 0.0 0.0 10-11 1.5497796988158134E-4 0.0 0.0 7.748898494079067E-5 0.0 12-13 1.5497796988158134E-4 0.0 0.0 2.32466954822372E-4 0.0 14-15 1.5497796988158134E-4 0.0 0.0 3.099559397631627E-4 0.0 16-17 1.5497796988158134E-4 0.0 0.0 3.099559397631627E-4 0.0 18-19 2.32466954822372E-4 0.0 0.0 3.099559397631627E-4 0.0 20-21 3.099559397631627E-4 0.0 0.0 4.64933909644744E-4 0.0 22-23 3.099559397631627E-4 0.0 0.0 6.974008644671161E-4 1.5497796988158134E-4 24-25 4.64933909644744E-4 0.0 0.0 0.0010848457891710693 1.5497796988158134E-4 26-27 4.64933909644744E-4 0.0 0.0 0.0016272686837566041 1.5497796988158134E-4 28-29 8.523788343486973E-4 0.0 0.0 0.004106916201861905 1.5497796988158134E-4 30-31 9.29867819289488E-4 0.0 0.0 0.008523788343486974 1.5497796988158134E-4 32-33 9.29867819289488E-4 0.0 0.0 0.01511035206345418 1.5497796988158134E-4 34-35 9.29867819289488E-4 0.0 0.0 0.021851893753302966 1.5497796988158134E-4 36-37 9.29867819289488E-4 0.0 0.0 0.028515946458210966 1.5497796988158134E-4 38-39 9.29867819289488E-4 0.0 0.0 0.03595488901252687 1.5497796988158134E-4 40-41 9.29867819289488E-4 0.0 0.0 0.04672585791929677 1.5497796988158134E-4 42-43 9.29867819289488E-4 0.0 0.0 0.06168123201286937 1.5497796988158134E-4 44-45 9.29867819289488E-4 0.0 0.0 0.0779539188504354 1.5497796988158134E-4 46-47 0.0010848457891710693 0.0 0.0 0.09562140741693569 1.5497796988158134E-4 48-49 0.0010848457891710693 0.0 0.0 0.11096422643521224 1.5497796988158134E-4 50-51 0.0010848457891710693 0.0 0.0 0.1301814947005283 1.5497796988158134E-4 52-53 0.0010848457891710693 0.0 0.0 0.17287792540290398 1.5497796988158134E-4 54-55 0.0012398237590526507 0.0 0.0 0.23417171249106938 1.5497796988158134E-4 56-57 0.0012398237590526507 0.0 0.0 0.2839971298079978 1.5497796988158134E-4 58-59 0.0012398237590526507 0.0 0.0 0.3583865553511568 1.5497796988158134E-4 60-61 0.0012398237590526507 0.0 0.0 0.4543954076927965 1.5497796988158134E-4 62-63 0.001394801728934232 0.0 0.0 0.509722542940521 1.5497796988158134E-4 64-65 0.001394801728934232 0.0 0.0 0.5576882246188704 1.5497796988158134E-4 66-67 0.001394801728934232 0.0 0.0 0.6985631992412279 1.5497796988158134E-4 68-69 0.001394801728934232 0.0 0.0 0.8513714775444671 1.5497796988158134E-4 70-71 0.001394801728934232 0.0 0.0 0.9166172028646128 1.5497796988158134E-4 72-73 0.001394801728934232 0.0 0.0 1.0469536755350226 1.5497796988158134E-4 74-75 0.001394801728934232 0.0 0.0 1.2307575478145782 1.5497796988158134E-4 76-77 0.001394801728934232 0.0 0.0 1.4161111997929494 1.5497796988158134E-4 78-79 0.001394801728934232 0.0 0.0 1.5721740154637018 1.5497796988158134E-4 80-81 0.0015497796988158134 0.0 0.0 1.753110795300448 1.5497796988158134E-4 82-83 0.0015497796988158134 0.0 0.0 1.9386194252487008 1.5497796988158134E-4 84-85 0.0017047576686973947 0.0 0.0 2.1624851027426453 1.5497796988158134E-4 86-87 0.0019372246235197666 0.0 0.0 2.5745715246577703 1.5497796988158134E-4 88-89 0.002014713608460557 0.0 0.0 3.0552356982454945 1.5497796988158134E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 605 0.0 38.4669 2 TATAACG 65 1.3283864E-4 36.534496 2 GTACCTG 740 0.0 31.451729 1 ATAACGC 80 4.4922938E-4 29.686579 3 GTATTAG 135 4.2214015E-7 28.147274 1 TAACGCA 85 6.405269E-4 27.94031 4 TACATGG 4460 0.0 27.368107 2 GTACATG 4540 0.0 27.097178 1 ACATGGG 4515 0.0 25.879597 3 GTAAGCG 65 9.997622E-6 25.585056 94-95 ACCTGGG 875 0.0 24.970654 3 GAGTACT 3255 0.0 24.884039 12-13 TATACAG 310 0.0 22.984941 5 GTACACG 125 2.2548858E-4 22.799294 1 CATGGGA 3045 0.0 22.462358 4 ATGGGAT 1070 0.0 22.197294 5 GTACTTT 3655 0.0 22.16075 14-15 CATGGGG 1265 0.0 22.153463 4 GTATAAT 360 0.0 21.110456 1 ATGGGGA 680 0.0 20.258295 5 >>END_MODULE