FastQCFastQC Report
Thu 26 May 2016
SRR936523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645253
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76141.1800022626783602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.8046456196251703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47260.7324258856603534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27660.428669064692454No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26980.4181305627405064No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24780.38403540936655856No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22250.34482598298651845No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21890.3392467760707815No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20040.310575851642689No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16870.26144783519022774No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16050.24873964165993803No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15880.24610501617195116No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15650.2425405228646748No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13780.21355964249681908No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.19868175738818727No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12480.1934125064122135No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10980.1701658109299763No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8980.13917021695366003No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC8490.13157629642946256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.129561582821002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.12770184718242303No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7690.11917805883893604No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6710.10399021779054107No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.10182052621219893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC600.00395155631.6635938
GTAAGCG1051.8189894E-1229.40304894-95
CGGTGTA650.005842756529.2301984
GTCCGAG700.00837701327.150741
TACCTGG5650.026.90632
GTACCTG6550.024.6636521
CTGGTCG1201.710809E-423.7476949
GAGTACT46900.023.04238112-13
GTACATG43800.022.563631
TACATGG44250.022.2233492
ACATGGG43250.021.6354163
AGTACTT47950.021.49759512-13
GTACTTT50350.021.3219914-15
ATGGGGC3252.1827873E-1120.4611385
TTAGTCG704.8540917E-420.35595538-39
CACCGTC1200.004763950619.7897457
GTATATG3850.019.7459961
CATGGGA29200.019.3574984
ACTTTTT57350.019.25637816-17
CATGGGG12100.019.235164
Produced by FastQC (version 0.11.5)