##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645253 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05870100565205 31.0 31.0 34.0 31.0 34.0 2 32.155275527583754 33.0 31.0 34.0 30.0 34.0 3 32.267130877345785 34.0 31.0 34.0 30.0 34.0 4 35.96847903070579 37.0 35.0 37.0 35.0 37.0 5 35.87156200746064 37.0 35.0 37.0 35.0 37.0 6 35.96010867055248 37.0 35.0 37.0 35.0 37.0 7 35.900792402360004 37.0 35.0 37.0 35.0 37.0 8 35.88866847577617 37.0 35.0 37.0 35.0 37.0 9 37.50131498807445 39.0 37.0 39.0 35.0 39.0 10-11 37.39999426581511 39.0 37.0 39.0 34.0 39.0 12-13 37.29453563175994 39.0 37.0 39.0 34.0 39.0 14-15 38.32811625827389 40.0 38.0 41.0 33.5 41.0 16-17 38.340338595868594 40.0 38.0 41.0 33.5 41.0 18-19 38.572983000466486 40.0 38.0 41.0 34.0 41.0 20-21 38.6067968688251 40.0 38.0 41.0 34.0 41.0 22-23 38.51448966529408 40.0 38.0 41.0 34.0 41.0 24-25 38.38872271806563 40.0 38.0 41.0 34.0 41.0 26-27 38.15787605791837 40.0 37.5 41.0 33.5 41.0 28-29 38.1594041407014 40.0 38.0 41.0 34.0 41.0 30-31 38.00201703827801 40.0 37.0 41.0 33.5 41.0 32-33 37.74044057137277 40.0 36.5 41.0 33.0 41.0 34-35 37.488191453584875 40.0 36.0 41.0 33.0 41.0 36-37 37.27062950501586 39.5 36.0 41.0 32.5 41.0 38-39 37.03778595372668 39.0 35.5 41.0 32.0 41.0 40-41 36.81104233533203 39.0 35.0 41.0 31.0 41.0 42-43 36.49943433040993 39.0 35.0 41.0 30.5 41.0 44-45 36.10113242402593 39.0 35.0 40.0 30.0 41.0 46-47 35.54333106548904 39.0 35.0 40.0 28.0 41.0 48-49 34.99632779700366 38.0 35.0 40.0 25.5 41.0 50-51 34.65420230514232 38.0 34.0 40.0 24.0 41.0 52-53 34.40470017187057 38.0 34.0 40.0 23.5 41.0 54-55 34.09725642499919 38.0 34.0 40.0 23.0 41.0 56-57 33.74551997433565 38.0 33.0 40.0 21.5 41.0 58-59 33.39324110077752 37.0 33.0 40.0 20.0 41.0 60-61 32.98173507135961 37.0 32.0 39.5 18.5 41.0 62-63 32.69177593905026 36.0 32.0 39.0 18.0 40.0 64-65 32.38355497765993 36.0 31.5 39.0 14.5 40.0 66-67 31.9182072768356 35.5 31.0 39.0 11.0 40.0 68-69 31.45880453093593 35.0 31.0 38.0 8.0 40.0 70-71 30.944755003076313 35.0 30.0 37.5 7.0 39.5 72-73 30.361359807703334 34.0 29.5 37.0 3.5 39.0 74-75 29.843483098877496 34.0 29.0 36.0 2.0 39.0 76-77 28.49257965480207 32.5 27.0 35.0 2.0 37.0 78-79 29.234019834080584 34.0 29.0 35.0 2.0 37.0 80-81 29.033614721667313 34.0 29.0 35.0 2.0 37.0 82-83 28.60311614204041 34.0 28.5 35.0 2.0 36.5 84-85 28.159353772861188 33.0 27.5 35.0 2.0 36.0 86-87 27.77095805831201 33.0 27.0 35.0 2.0 36.0 88-89 27.243635442221887 33.0 26.0 35.0 2.0 35.0 90-91 26.644581272772076 32.0 25.0 34.5 2.0 35.0 92-93 26.094077826836916 32.0 24.5 34.0 2.0 35.0 94-95 25.36920556742859 32.0 21.5 34.0 2.0 35.0 96-97 24.543962600716306 31.0 17.0 34.0 2.0 35.0 98-99 23.44913080605592 31.0 2.0 34.0 2.0 35.0 100-101 19.745994981813336 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2401920091259484 1101 2 0.342093320049095 1101 3 0.2948933499732007 1101 4 0.2791370716383881 1101 5 0.31171442478490974 1101 6 0.11426032996651969 1101 7 0.20776635170950186 1101 8 0.2241462062346713 1101 9 0.18658574910496384 1101 10-11 0.1674662709020467 1101 12-13 0.3545303558055295 1101 14-15 0.3437104576520724 1101 16-17 0.3439007545448547 1101 18-19 0.33365577366212307 1101 20-21 0.220207667323443 1101 22-23 0.2630615715498479 1101 24-25 0.3548551733357641 1101 26-27 0.2204024669090927 1101 28-29 0.2985659851617015 1101 30-31 0.5997110802714829 1101 32-33 0.45498263012434137 1101 34-35 0.4770680986821674 1101 36-37 0.449414321603534 1101 38-39 0.5657653230726964 1101 40-41 0.6478567372418098 1101 42-43 0.6320657425349339 1101 44-45 0.6647750132142107 1101 46-47 0.6556274195365077 1101 48-49 0.8025984635168015 1101 50-51 0.7305720243011109 1101 52-53 0.5912578520294005 1101 54-55 0.48272020651221936 1101 56-57 0.6874699380134857 1101 58-59 0.9298979834355023 1101 60-61 1.0341162553254506 1101 62-63 0.7968865280297663 1101 64-65 0.5798854535839553 1101 66-67 0.7498949264466965 1101 68-69 0.652904677456533 1101 70-71 0.6206035477298073 1101 72-73 0.7723121798277823 1101 74-75 0.8747991636251982 1101 76-77 0.8970403740632875 1101 78-79 0.9743241942004204 1101 80-81 0.9611382831260933 1101 82-83 0.8395347959267951 1101 84-85 0.7273719854542051 1101 86-87 0.6767924751882184 1101 88-89 0.5532652737999726 1101 90-91 0.6923623413747002 1101 92-93 1.043329382795779 1101 94-95 0.9259182464196485 1101 96-97 0.9451175206738185 1101 98-99 0.8037686619270445 1101 100-101 0.9871618577359769 1102 1 0.20107440346644267 1102 2 0.33733623133525725 1102 3 0.16056359119018282 1102 4 0.17688442721838982 1102 5 0.08569145521463639 1102 6 0.15322414481280333 1102 7 0.1885425000576859 1102 8 0.0027604206764095807 1102 9 0.06862819536940634 1102 10-11 0.17665983358353543 1102 12-13 0.29252528852857296 1102 14-15 0.33468762883949665 1102 16-17 0.353843721612769 1102 18-19 0.3818913499084786 1102 20-21 0.1918554185889576 1102 22-23 0.2675892773558388 1102 24-25 0.20785070558230956 1102 26-27 0.22552317966434288 1102 28-29 0.10863433084173835 1102 30-31 0.27164529950940874 1102 32-33 0.32085488469765977 1102 34-35 0.26670702913222755 1102 36-37 0.3528402074646806 1102 38-39 0.19938086689265333 1102 40-41 0.49446734411600346 1102 42-43 0.584811560206802 1102 44-45 0.6963738120818945 1102 46-47 0.6350533366771387 1102 48-49 0.6395189458464969 1102 50-51 0.3686240420887117 1102 52-53 0.6554016542596273 1102 54-55 0.4459941783293928 1102 56-57 0.5707674737112001 1102 58-59 0.6509383913200253 1102 60-61 0.3198087571109838 1102 62-63 0.4432458563548991 1102 64-65 0.42479132839302025 1102 66-67 0.5262439846734601 1102 68-69 0.5333740261654754 1102 70-71 0.1920745698913251 1102 72-73 0.18668849291459821 1102 74-75 0.36710049978064063 1102 76-77 0.2994053639756551 1102 78-79 0.3379964581575905 1102 80-81 0.44727104369725623 1102 82-83 0.5498476429336066 1102 84-85 0.3030266390599472 1102 86-87 0.3663592021547828 1102 88-89 0.5303973618621569 1102 90-91 0.5077859959657509 1102 92-93 0.5998879093233889 1102 94-95 0.6463734672267663 1102 96-97 0.5662994682389737 1102 98-99 0.7008226555356032 1102 100-101 0.7965055619571437 1103 1 0.09020890767830281 1103 2 0.13379331441624487 1103 3 0.2038411334464172 1103 4 0.08593028968604699 1103 5 0.07572614111454357 1103 6 0.12816220570582715 1103 7 0.23053996743642102 1103 8 0.04968273527201461 1103 9 -0.060885100892221544 1103 10-11 0.16315573183822352 1103 12-13 0.07355953397260606 1103 14-15 0.3409503607670459 1103 16-17 0.3886424883363233 1103 18-19 0.2759796062538058 1103 20-21 0.2971298776541005 1103 22-23 0.2514438168041693 1103 24-25 0.3065816421016052 1103 26-27 0.36981101757658763 1103 28-29 0.4071546743973258 1103 30-31 0.4121286998287488 1103 32-33 0.3076357028444434 1103 34-35 0.2355117420100541 1103 36-37 0.42666887013054833 1103 38-39 0.4318851338089118 1103 40-41 0.48685296323178306 1103 42-43 0.6271200995450101 1103 44-45 0.554845705491573 1103 46-47 0.47590286593992914 1103 48-49 0.6098831477955216 1103 50-51 0.6505447049747914 1103 52-53 0.6541697542431137 1103 54-55 0.48014740191580785 1103 56-57 0.6285263791783606 1103 58-59 0.30613439418298327 1103 60-61 0.19395262101011923 1103 62-63 0.5208486752724824 1103 64-65 0.6049474149425933 1103 66-67 0.14581674247778764 1103 68-69 0.4272090203844918 1103 70-71 0.42091617094841993 1103 72-73 0.6663779715249589 1103 74-75 0.7087483553111156 1103 76-77 0.7199801588883901 1103 78-79 0.5002493899419846 1103 80-81 0.9907220154530201 1103 82-83 0.8301744061335192 1103 84-85 0.8800926523837376 1103 86-87 1.1410618944413038 1103 88-89 0.7877777505644623 1103 90-91 0.7107521909775869 1103 92-93 0.8830184494324023 1103 94-95 1.3250113574431381 1103 96-97 1.1793314563466666 1103 98-99 1.0280166763471428 1103 100-101 0.822185515709279 1104 1 -0.041432574546284684 1104 2 0.052305446838339265 1104 3 -0.05668617234078255 1104 4 -0.011066387221596585 1104 5 -0.11716996522441292 1104 6 0.009508998608204422 1104 7 -0.09050837415239243 1104 8 -0.16730579065437468 1104 9 -0.12775136209982207 1104 10-11 0.003275802062070454 1104 12-13 0.09710176232547241 1104 14-15 0.04740531092065936 1104 16-17 -0.011705495863303383 1104 18-19 0.03925786821379518 1104 20-21 -0.02731366112576694 1104 22-23 -0.08918882474075218 1104 24-25 -0.19028336510887556 1104 26-27 -0.08276281150360632 1104 28-29 -0.0363574580247672 1104 30-31 -0.15076239052174856 1104 32-33 0.0361954871569381 1104 34-35 -0.2574157142568012 1104 36-37 -0.046235387189661026 1104 38-39 -0.11275246102461978 1104 40-41 -0.16297461216478837 1104 42-43 -0.25107602560390063 1104 44-45 -0.10667069052071554 1104 46-47 -0.1505345282540631 1104 48-49 -0.1415542463984778 1104 50-51 0.02288309609649275 1104 52-53 -0.058688418683097154 1104 54-55 -0.06088808620256714 1104 56-57 0.06954305733999888 1104 58-59 0.12128111184554058 1104 60-61 0.047299225812928114 1104 62-63 -0.055770548503303985 1104 64-65 0.11024398526048884 1104 66-67 0.2455001082060413 1104 68-69 0.32742690238336536 1104 70-71 -0.056674900592113886 1104 72-73 -0.19877344862677404 1104 74-75 0.14885118307460843 1104 76-77 -0.1037047116364107 1104 78-79 -0.28652407547904346 1104 80-81 -0.4925120431086434 1104 82-83 -0.7108297320569648 1104 84-85 -0.6308954973435199 1104 86-87 -0.40162259988436233 1104 88-89 -0.2818911941969624 1104 90-91 0.03124720633905298 1104 92-93 -0.2093541131107024 1104 94-95 -0.4242393792801664 1104 96-97 -0.4275247728840057 1104 98-99 -0.15044122069969035 1104 100-101 -0.2083979064488517 1105 1 0.10422616163497622 1105 2 0.2220565006891988 1105 3 0.22002781872028976 1105 4 0.10136052851378707 1105 5 0.07347424603381825 1105 6 0.06506473988071804 1105 7 0.02162436592669792 1105 8 0.04393405944976081 1105 9 0.11704791315833063 1105 10-11 0.07402588312751845 1105 12-13 -0.04492741455097615 1105 14-15 0.1812878278608281 1105 16-17 0.029920359502561666 1105 18-19 0.13174017588810472 1105 20-21 0.04424206008931719 1105 22-23 -0.0305743252238031 1105 24-25 0.10195257045327821 1105 26-27 0.2357090796781378 1105 28-29 0.030237391895497012 1105 30-31 0.25661155688098347 1105 32-33 0.31803248041430265 1105 34-35 0.09354085806194234 1105 36-37 -0.0535588567241021 1105 38-39 0.1747908713838484 1105 40-41 0.16889647872545765 1105 42-43 0.13919311169256332 1105 44-45 -0.039591955074889995 1105 46-47 0.025339892169746747 1105 48-49 -0.23811155549352492 1105 50-51 -0.09202007665481915 1105 52-53 -0.02061774298166341 1105 54-55 -0.03608289400223441 1105 56-57 0.13325298008545872 1105 58-59 0.1684444896506463 1105 60-61 -0.23054466720073208 1105 62-63 0.1640898183471151 1105 64-65 -0.11198065128214552 1105 66-67 -0.12838795795673263 1105 68-69 -0.20215362987399743 1105 70-71 -0.1421638506190277 1105 72-73 0.083246568689475 1105 74-75 0.4134947792382917 1105 76-77 -0.07874323861867083 1105 78-79 -0.019058231612774534 1105 80-81 0.21060371166920433 1105 82-83 0.3093848155978236 1105 84-85 0.16099277607938234 1105 86-87 0.09182864885787012 1105 88-89 0.15166020798461588 1105 90-91 0.4399800446995563 1105 92-93 0.17767574906131856 1105 94-95 0.29286476224506686 1105 96-97 0.3671188901377782 1105 98-99 0.1322072627913684 1105 100-101 0.2871217648767086 1106 1 0.02259412485413037 1106 2 0.2166227951058275 1106 3 0.17079040598703443 1106 4 0.05805319669160269 1106 5 0.034237946842175404 1106 6 -0.06704470427328602 1106 7 0.09308731424516026 1106 8 0.09353384504581186 1106 9 0.07129833922954987 1106 10-11 -0.0254766091295906 1106 12-13 0.04910645598754115 1106 14-15 0.015806107873139297 1106 16-17 0.11430888360695945 1106 18-19 0.1315948492712522 1106 20-21 0.26108258394458517 1106 22-23 0.1631309561719405 1106 24-25 0.2343932460521927 1106 26-27 0.13930374298279702 1106 28-29 0.23437599196608971 1106 30-31 0.22209365434040507 1106 32-33 0.1371801114960789 1106 34-35 0.3705182909144398 1106 36-37 0.4638878867328984 1106 38-39 0.29044915377421887 1106 40-41 0.2426195390397794 1106 42-43 0.4425879037150722 1106 44-45 0.452664212341368 1106 46-47 0.571771746254278 1106 48-49 0.5031032617818525 1106 50-51 0.1524447722338138 1106 52-53 0.22716791346999088 1106 54-55 0.1811200027158364 1106 56-57 0.5211429319766907 1106 58-59 0.34364988145686937 1106 60-61 0.2873550423347737 1106 62-63 0.231112755416909 1106 64-65 0.23751759441797304 1106 66-67 0.21755733506513408 1106 68-69 0.20671714456587864 1106 70-71 0.5547669267273818 1106 72-73 0.5104237784561789 1106 74-75 0.6847280360077761 1106 76-77 0.4944872210852793 1106 78-79 0.40644960941877883 1106 80-81 0.24413452563037552 1106 82-83 0.1609657615797282 1106 84-85 0.1828644319157533 1106 86-87 0.3405636467157187 1106 88-89 0.47664638308242857 1106 90-91 0.38692922379290806 1106 92-93 0.33631768126361905 1106 94-95 0.530981480279344 1106 96-97 0.7536084219029675 1106 98-99 0.8232315512099717 1106 100-101 0.6661968081444058 1107 1 -0.01593220730427447 1107 2 -0.062098090926376415 1107 3 0.011942178742586407 1107 4 0.0389282571657219 1107 5 -0.18890074622576236 1107 6 -0.06232124377152104 1107 7 -0.16765980027554406 1107 8 -0.13473218923437003 1107 9 0.011066118619972087 1107 10-11 -0.04118152135573183 1107 12-13 -0.07235364837350033 1107 14-15 -0.20734014445944382 1107 16-17 0.0367551480343522 1107 18-19 0.16245608709003534 1107 20-21 -0.1416005230427686 1107 22-23 0.0032222720887205014 1107 24-25 -0.052085605285157044 1107 26-27 -0.028047577080791086 1107 28-29 0.038611555930430086 1107 30-31 0.12324223060691253 1107 32-33 -0.02437649227750427 1107 34-35 0.04423343190552487 1107 36-37 0.07356201763344217 1107 38-39 0.13969581905862327 1107 40-41 0.15657566938742207 1107 42-43 -0.07855643936323276 1107 44-45 -0.1385231969474532 1107 46-47 -0.03244135589514485 1107 48-49 0.14320559262710475 1107 50-51 0.1321395075299634 1107 52-53 0.1380366241618063 1107 54-55 0.04534049134669971 1107 56-57 -0.0430147067682114 1107 58-59 0.03463686224338147 1107 60-61 0.1798774177580853 1107 62-63 0.05992024902393922 1107 64-65 0.18094189810171457 1107 66-67 0.029211777320991672 1107 68-69 -0.10763055568983759 1107 70-71 -0.3007301705149956 1107 72-73 -0.5391207218548288 1107 74-75 -0.28440640659364647 1107 76-77 -0.5586965251998777 1107 78-79 -0.26425046487799264 1107 80-81 -0.03934113785708249 1107 82-83 -0.02039179595707452 1107 84-85 -0.04434114552567081 1107 86-87 0.3833782417119167 1107 88-89 0.27027629876423376 1107 90-91 0.1660766683295023 1107 92-93 0.015241155588036293 1107 94-95 0.07824524246056086 1107 96-97 0.4161563692386494 1107 98-99 0.7050165311671215 1107 100-101 0.9221891452098561 1108 1 -0.12292269974698655 1108 2 0.18510855623760847 1108 3 -0.03236547605995099 1108 4 0.01521523099962252 1108 5 0.11988663391400678 1108 6 -0.07739011261598705 1108 7 -0.03719498636363028 1108 8 0.04679003569656004 1108 9 -0.15787586090220884 1108 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-1.115319467083296 2316 50-51 -1.0112470986799167 2316 52-53 -1.0215604239050649 2316 54-55 -0.6836629046825564 2316 56-57 -0.7082329296372976 2316 58-59 -0.7903160225155332 2316 60-61 -0.8328250378322934 2316 62-63 -0.9313074528718701 2316 64-65 -0.7351416591456328 2316 66-67 -0.8666192235746628 2316 68-69 -1.0397169678409064 2316 70-71 -0.9047662206717355 2316 72-73 -0.6335914450971032 2316 74-75 -0.9026936509584793 2316 76-77 -0.8209021715650096 2316 78-79 -0.9088032899265066 2316 80-81 -0.6500456273331245 2316 82-83 -0.602540250950355 2316 84-85 -0.6949513112220451 2316 86-87 -0.9559657835157296 2316 88-89 -0.9356452940108078 2316 90-91 -0.9653258730945886 2316 92-93 -0.9204068888962134 2316 94-95 -1.1386364749599522 2316 96-97 -1.0760206347918029 2316 98-99 -0.21410619237370554 2316 100-101 -0.7905734872726029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 7.0 5 20.0 6 35.0 7 93.0 8 231.0 9 497.0 10 878.0 11 1410.0 12 2216.0 13 3120.0 14 4093.0 15 5478.0 16 7702.0 17 10789.0 18 9177.0 19 5181.0 20 4383.0 21 4402.0 22 4532.0 23 5095.0 24 5642.0 25 6725.0 26 8079.0 27 9667.0 28 12119.0 29 14979.0 30 18327.0 31 23072.0 32 29250.0 33 37913.0 34 50936.0 35 69890.0 36 98309.0 37 122498.0 38 65729.0 39 2772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72565382701736 19.61550801890375 14.865700605893394 19.793137548185495 2 15.530359979850427 22.69725268338048 39.094354244972294 22.6780330917968 3 21.352981655641866 26.624427320012643 30.643881390922672 21.37870963342281 4 12.393335916311507 17.48857032158078 38.79674544750097 31.32134831460674 5 12.027854108449999 39.70992794694514 33.955757657477534 14.306460287127317 6 28.98397992725334 37.93519751167372 17.536687159920216 15.544135401152726 7 26.98961026079733 35.186407791064575 20.781803078487165 17.042178869650925 8 26.38629727668913 33.025646112168424 19.666358772805324 20.921697838337124 9 26.768724825766014 17.720181076260012 21.26793676278917 34.243157335184804 10-11 24.85900879190023 27.046445347793814 26.72773315273234 21.36681270757362 12-13 27.861474491401044 24.943394296500752 27.270311025287757 19.924820186810443 14-15 23.610342673913483 26.01104379859558 28.298610303929177 22.080003223561757 16-17 20.35778078274717 29.29106400455653 28.888570310471888 21.46258490222441 18-19 19.88353216038931 28.158328070454132 32.17185786739094 19.786281901765616 20-21 21.751525380124573 25.783147281127132 31.875136575182605 20.590190763565694 22-23 20.886552163687856 26.108538905312262 31.8781407278546 21.12676820314528 24-25 21.68753588733643 26.090955655069326 32.345987631121965 19.875520826472282 26-27 21.431842573256205 25.404318464477083 33.388867148906826 19.774971813359887 28-29 20.088492489374314 27.24096380874154 32.953533694173984 19.717010007710158 30-31 20.536071290197597 26.2664858581945 32.44223169314219 20.755211158465713 32-33 20.519703332204543 26.697776851325916 32.31566556913118 20.466854247338368 34-35 21.682007045364024 25.86172426993074 32.2581120098195 20.19815667488574 36-37 21.735656882580898 25.41842406452708 32.785120771299745 20.060798281592284 38-39 21.06892139973854 26.19003890917911 32.9605150487314 19.78052464235095 40-41 20.861507036774725 26.75415689659715 32.40070174024762 19.983634326380507 42-43 20.329408482791486 27.323158942886256 31.256765814668015 21.09066675965424 44-45 21.169732306700393 26.40193361602936 30.939018871199725 21.489315206070525 46-47 22.105747667969 27.411418466864934 30.131669283211394 20.351164581954677 48-49 22.118686884341425 27.395062952929432 29.679857542263083 20.806392620466056 50-51 21.02663965867017 27.91499610223157 30.60282902047452 20.455535218623737 52-53 21.72394928485194 28.62831673255834 29.115878937033806 20.531855045555915 54-55 20.707784936904545 27.80733123854615 29.650718129724414 21.834165694824893 56-57 21.912434271454533 27.84844363329187 29.049362767321917 21.189759327931675 58-59 23.079134549889847 27.143303269421367 28.666000760961293 21.111561419727494 60-61 21.49201453733911 27.6238889706851 30.443869287933857 20.44022720404194 62-63 21.595792658073655 27.442026615916546 30.20342408326656 20.758756642743233 64-65 21.164724735522306 28.787308170369197 29.451175852481427 20.59679124162707 66-67 21.451525039694193 27.77555209606266 28.981139736735862 21.791783127507284 68-69 21.060931288600276 28.256061744945637 29.11210644183902 21.570900524615062 70-71 21.948407104813906 28.282636508688064 28.637241766237953 21.13171462026008 72-73 21.89929839298362 27.16468314546838 29.60883897754765 21.327179484000354 74-75 21.076679386118542 29.326525171805596 28.751747503897978 20.845047938177885 76-77 21.709034130314336 27.872638018744745 28.784613656000218 21.6337141949407 78-79 21.670365743653967 27.954095281197013 28.808551067411024 21.566987907737996 80-81 22.065845573022006 28.14585958337789 28.604683240421735 21.183611603178367 82-83 21.733449882366067 28.031049778215873 29.05046635111636 21.1850339883017 84-85 21.214175898430963 28.381320943034797 28.835573340607155 21.568929817927085 86-87 21.284512865973344 28.62331334361878 28.38425815790351 21.707915632504378 88-89 21.365056216083016 28.47976740203353 27.88145686794006 22.2737195139434 90-91 21.44496728136169 27.63949981246067 28.367725876397632 22.547807029780007 92-93 21.694687508717767 28.432398168726387 28.420309531115844 21.452604791440006 94-95 21.43185366855354 28.16655624392191 28.4087888755865 21.992801211938055 96-97 20.51189263117927 28.101479653314016 29.18578385980682 22.2008438556999 98-99 21.02267575980417 28.755820431973106 27.674144147564856 22.54735966065787 100-101 20.480862443460655 28.66855796285548 27.880009887847322 22.970569705836542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 475.0 1 371.5 2 375.5 3 483.0 4 387.0 5 318.0 6 501.0 7 918.5 8 1417.5 9 1683.0 10 1532.0 11 1309.0 12 1334.5 13 1425.0 14 1482.0 15 1569.5 16 1673.5 17 1777.0 18 1854.5 19 1964.5 20 2139.5 21 2330.5 22 2586.0 23 2927.0 24 3280.5 25 3873.5 26 4732.5 27 5754.5 28 6657.5 29 7754.5 30 9217.0 31 10569.5 32 12301.5 33 13833.5 34 14895.5 35 15811.5 36 17246.5 37 18572.0 38 18692.5 39 18699.5 40 19011.0 41 19415.5 42 19554.0 43 19718.0 44 20505.0 45 21180.5 46 21556.0 47 26196.5 48 39592.5 49 38201.0 50 24336.0 51 19078.5 52 16661.0 53 15655.0 54 15168.0 55 14214.0 56 12573.0 57 11379.0 58 10762.0 59 9690.0 60 8021.0 61 6590.0 62 5498.5 63 4258.5 64 3023.0 65 2207.0 66 1616.0 67 1206.0 68 895.0 69 629.0 70 468.0 71 379.5 72 342.0 73 323.5 74 230.0 75 181.0 76 148.5 77 85.0 78 51.5 79 45.5 80 40.0 81 26.0 82 17.5 83 17.0 84 8.5 85 3.0 86 3.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01394801728934232 2 0.012088281650763344 3 0.006354096765144834 4 4.64933909644744E-4 5 0.005269250975973765 6 0.0 7 1.5497796988158131E-4 8 0.001394801728934232 9 0.0 10-11 0.0 12-13 0.0 14-15 6.199118795263253E-4 16-17 0.003951938231980324 18-19 0.001627268683756604 20-21 6.199118795263253E-4 22-23 7.748898494079066E-5 24-25 6.97400864467116E-4 26-27 7.748898494079066E-5 28-29 7.748898494079066E-5 30-31 4.64933909644744E-4 32-33 0.003022070412690836 34-35 9.29867819289488E-4 36-37 6.97400864467116E-4 38-39 0.005036784021151393 40-41 0.0 42-43 8.523788343486973E-4 44-45 0.00573418488561851 46-47 0.0 48-49 0.001394801728934232 50-51 0.001859735638578976 52-53 3.0995593976316263E-4 54-55 7.748898494079066E-5 56-57 0.012475726575467298 58-59 0.002557136503046092 60-61 0.002789603457868464 62-63 0.0 64-65 0.0029445814277500454 66-67 0.00116233477411186 68-69 0.002789603457868464 70-71 0.004416872141625068 72-73 0.003099559397631627 74-75 0.0072839645844343225 76-77 8.523788343486973E-4 78-79 0.0072839645844343225 80-81 0.002479647518105301 82-83 0.004804317066329022 84-85 0.0026346254879868825 86-87 6.97400864467116E-4 88-89 0.003022070412690836 90-91 0.008523788343486972 92-93 0.0029445814277500454 94-95 8.523788343486973E-4 96-97 7.748898494079066E-5 98-99 6.97400864467116E-4 100-101 0.002557136503046092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 645253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.17354044555078 #Duplication Level Percentage of deduplicated Percentage of total 1 82.66026544418996 48.086402950575476 2 10.489106207977745 12.20376888454942 3 2.872523111790379 5.013145182735509 4 1.2346333361297148 2.8729196925906897 5 0.6415707059140009 1.8661219704584346 6 0.389346766471344 1.3589807919999082 7 0.2931579557863032 1.1937825338508647 8 0.19408426849311303 0.9032455234423398 9 0.14721586644104998 0.7707661344571699 >10 0.9594344937039299 10.285180568725245 >50 0.07071497324925927 2.8509464030794742 >100 0.03937536239345712 4.28732550816482 >500 0.0040178941217774945 1.5535594721371084 >1k 0.0040178941217774945 4.7583796991625755 >5k 5.357192162369992E-4 1.9954746840709943 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7614 1.1800022626783602 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5192 0.8046456196251703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4726 0.7324258856603534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2766 0.428669064692454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2698 0.4181305627405064 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2478 0.38403540936655856 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2225 0.34482598298651845 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2189 0.3392467760707815 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2004 0.310575851642689 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1687 0.26144783519022774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1605 0.24873964165993803 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1588 0.24610501617195116 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1565 0.2425405228646748 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1378 0.21355964249681908 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.19868175738818727 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1248 0.1934125064122135 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1098 0.1701658109299763 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 898 0.13917021695366003 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 849 0.13157629642946256 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 836 0.129561582821002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 824 0.12770184718242303 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 769 0.11917805883893604 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 671 0.10399021779054107 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.10182052621219893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.748898494079067E-5 0.0 0.0 0.0 0.0 14-15 1.5497796988158134E-4 0.0 0.0 7.748898494079067E-5 0.0 16-17 2.32466954822372E-4 0.0 0.0 1.5497796988158134E-4 1.5497796988158134E-4 18-19 3.8744492470395335E-4 0.0 0.0 1.5497796988158134E-4 1.5497796988158134E-4 20-21 4.64933909644744E-4 0.0 0.0 4.64933909644744E-4 1.5497796988158134E-4 22-23 6.199118795263254E-4 0.0 0.0 6.974008644671161E-4 1.5497796988158134E-4 24-25 6.199118795263254E-4 0.0 0.0 0.0010848457891710693 1.5497796988158134E-4 26-27 6.199118795263254E-4 0.0 0.0 0.0016272686837566041 1.5497796988158134E-4 28-29 6.199118795263254E-4 0.0 0.0 0.004106916201861905 1.5497796988158134E-4 30-31 6.199118795263254E-4 0.0 0.0 0.008756255298309345 1.5497796988158134E-4 32-33 6.199118795263254E-4 0.0 0.0 0.015730263942980504 1.5497796988158134E-4 34-35 6.199118795263254E-4 0.0 0.0 0.022704272587651664 1.5497796988158134E-4 36-37 6.199118795263254E-4 0.0 0.0 0.029445814277500454 1.5497796988158134E-4 38-39 6.199118795263254E-4 0.0 0.0 0.03711722378663873 1.5497796988158134E-4 40-41 6.199118795263254E-4 0.0 0.0 0.04812065964823101 1.5497796988158134E-4 42-43 6.974008644671161E-4 0.0 0.0 0.06315352272674439 1.5497796988158134E-4 44-45 9.29867819289488E-4 0.0 0.0 0.08012361042877755 1.5497796988158134E-4 46-47 0.0010848457891710693 0.0 0.0 0.09771361001033703 1.5497796988158134E-4 48-49 0.0010848457891710693 0.0 0.0 0.11313391801355438 1.5497796988158134E-4 50-51 0.0010848457891710693 0.0 0.0 0.1329710981583968 1.5497796988158134E-4 52-53 0.0010848457891710693 0.0 0.0 0.1765973966800619 4.64933909644744E-4 54-55 0.0010848457891710693 0.0 0.0 0.2382011397079905 4.64933909644744E-4 56-57 0.0012398237590526507 0.0 0.0 0.2883365129646821 4.64933909644744E-4 58-59 0.0012398237590526507 0.0 0.0 0.36373329531207144 4.64933909644744E-4 60-61 0.0012398237590526507 0.0 0.0 0.4609044824278229 4.64933909644744E-4 62-63 0.0012398237590526507 0.0 0.0 0.5162316176755475 4.64933909644744E-4 64-65 0.0012398237590526507 0.0 0.0 0.5647397222484823 4.64933909644744E-4 66-67 0.0012398237590526507 0.0 0.0 0.7056146968708399 4.64933909644744E-4 68-69 0.0012398237590526507 0.0 0.0 0.8591978650234868 4.64933909644744E-4 70-71 0.0012398237590526507 0.0 0.0 0.9249085242532774 4.64933909644744E-4 72-73 0.0013173127439934412 0.0 0.0 1.0556324418483913 4.64933909644744E-4 74-75 0.001394801728934232 0.0 0.0 1.238971380218302 4.64933909644744E-4 76-77 0.001394801728934232 0.0 0.0 1.4256423449406666 6.199118795263254E-4 78-79 0.001394801728934232 0.0 0.0 1.5817051606114192 6.199118795263254E-4 80-81 0.0015497796988158134 0.0 0.0 1.7634168302975732 6.974008644671161E-4 82-83 0.0015497796988158134 0.0 0.0 1.9503202619747602 7.748898494079067E-4 84-85 0.0016272686837566041 0.0 0.0 2.1727136487548293 7.748898494079067E-4 86-87 0.0017047576686973947 0.0 0.0 2.583715224880783 9.29867819289488E-4 88-89 0.0017047576686973947 0.0 0.0 3.0600400153118237 0.0010848457891710693 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 60 0.003951556 31.663593 8 GTAAGCG 105 1.8189894E-12 29.403048 94-95 CGGTGTA 65 0.0058427565 29.230198 4 GTCCGAG 70 0.008377013 27.15074 1 TACCTGG 565 0.0 26.9063 2 GTACCTG 655 0.0 24.663652 1 CTGGTCG 120 1.710809E-4 23.747694 9 GAGTACT 4690 0.0 23.042381 12-13 GTACATG 4380 0.0 22.56363 1 TACATGG 4425 0.0 22.223349 2 ACATGGG 4325 0.0 21.635416 3 AGTACTT 4795 0.0 21.497595 12-13 GTACTTT 5035 0.0 21.32199 14-15 ATGGGGC 325 2.1827873E-11 20.461138 5 TTAGTCG 70 4.8540917E-4 20.355955 38-39 CACCGTC 120 0.0047639506 19.789745 7 GTATATG 385 0.0 19.745996 1 CATGGGA 2920 0.0 19.357498 4 ACTTTTT 5735 0.0 19.256378 16-17 CATGGGG 1210 0.0 19.23516 4 >>END_MODULE