##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936522_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 723914 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543311774603062 31.0 31.0 34.0 30.0 34.0 2 31.764241332533974 31.0 31.0 34.0 30.0 34.0 3 32.04230198614753 33.0 31.0 34.0 30.0 34.0 4 35.83292766820368 37.0 35.0 37.0 35.0 37.0 5 35.69652610669223 37.0 35.0 37.0 33.0 37.0 6 35.82771710451794 37.0 35.0 37.0 35.0 37.0 7 35.77412372187857 37.0 35.0 37.0 33.0 37.0 8 35.760728484322726 37.0 35.0 37.0 33.0 37.0 9 37.39285467610794 39.0 37.0 39.0 34.0 39.0 10-11 37.28560574874916 39.0 37.0 39.0 34.0 39.0 12-13 37.17028058581544 39.0 37.0 39.0 33.0 39.0 14-15 38.216007702572405 40.0 37.5 41.0 33.0 41.0 16-17 38.16830866649906 40.0 37.5 41.0 33.0 41.0 18-19 38.306797492519834 40.0 38.0 41.0 33.5 41.0 20-21 38.40938702663576 40.0 38.0 41.0 34.0 41.0 22-23 38.3281025094141 40.0 38.0 41.0 34.0 41.0 24-25 38.21033575811491 40.0 37.5 41.0 33.5 41.0 26-27 37.9702782374702 40.0 37.0 41.0 33.0 41.0 28-29 37.87907058020704 40.0 37.0 41.0 33.0 41.0 30-31 37.83135220481991 40.0 37.0 41.0 33.0 41.0 32-33 37.582684545401804 40.0 36.0 41.0 33.0 41.0 34-35 37.36472564420636 39.0 36.0 41.0 33.0 41.0 36-37 37.090374685390806 39.0 35.5 41.0 31.5 41.0 38-39 36.901432352461754 39.0 35.0 41.0 31.0 41.0 40-41 36.65771555737284 39.0 35.0 41.0 31.0 41.0 42-43 36.36657738350135 39.0 35.0 40.5 30.0 41.0 44-45 35.9961328279326 39.0 35.0 40.0 30.0 41.0 46-47 35.43892920982327 39.0 35.0 40.0 27.5 41.0 48-49 34.83926060277878 38.0 34.5 40.0 24.0 41.0 50-51 34.61489071906331 38.0 34.0 40.0 24.0 41.0 52-53 34.35460496688833 38.0 34.0 40.0 23.0 41.0 54-55 34.10069980688314 38.0 34.0 40.0 23.0 41.0 56-57 33.80941382539915 38.0 33.0 40.0 22.0 41.0 58-59 33.45429636669549 37.5 33.0 40.0 20.0 41.0 60-61 33.03826490439472 37.0 32.0 40.0 18.0 41.0 62-63 32.78396259776714 36.5 32.0 39.0 18.0 40.5 64-65 32.34999046848107 36.0 31.5 39.0 14.0 40.0 66-67 31.92581093886843 35.5 31.0 39.0 12.5 40.0 68-69 31.47973170846261 35.0 31.0 38.0 8.0 40.0 70-71 30.989718391963684 35.0 30.0 37.5 7.5 39.5 72-73 30.56172211063745 34.0 30.0 37.0 6.5 39.0 74-75 30.19601292418713 34.0 29.5 36.5 2.0 39.0 76-77 28.79231441856353 32.5 28.0 35.0 2.0 37.5 78-79 29.55712971430308 34.0 29.0 36.0 2.0 37.5 80-81 29.36272955074774 34.0 29.0 35.0 2.0 37.0 82-83 29.003072188132844 34.0 29.0 35.0 2.0 37.0 84-85 28.55528211914675 34.0 29.0 35.0 2.0 36.0 86-87 28.193904248294686 34.0 28.0 35.0 2.0 36.0 88-89 27.741938959600173 33.0 27.0 35.0 2.0 35.5 90-91 27.251099578126684 33.0 26.0 35.0 2.0 35.0 92-93 26.829797462129477 33.0 25.0 35.0 2.0 35.0 94-95 26.300240774456633 32.5 25.0 34.0 2.0 35.0 96-97 25.58638457054291 32.0 23.0 34.0 2.0 35.0 98-99 24.760056443168665 32.0 17.0 34.0 2.0 35.0 100-101 22.00349972510547 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2666708650605294 1101 2 0.3157872662175656 1101 3 0.13456798328239472 1101 4 0.020990223902124683 1101 5 0.14125445001088366 1101 6 0.08752464509099411 1101 7 0.18950551692145723 1101 8 0.02362753626105274 1101 9 0.11784670171363132 1101 10-11 0.19565837345820825 1101 12-13 0.2094873432554749 1101 14-15 0.13631501931153167 1101 16-17 0.16233741774067312 1101 18-19 0.35213225464570996 1101 20-21 0.09764215783674501 1101 22-23 0.01296190262190322 1101 24-25 0.07033708262070348 1101 26-27 0.09975992563828129 1101 28-29 0.06033332923259849 1101 30-31 0.005770962237996002 1101 32-33 0.07649605571302942 1101 34-35 0.15445990968194678 1101 36-37 -0.021758535719690997 1101 38-39 0.03820376973332884 1101 40-41 0.2615668120888941 1101 42-43 0.42349806190489403 1101 44-45 0.13525144651556786 1101 46-47 0.27712833405173853 1101 48-49 0.23523643843882525 1101 50-51 0.13459602917979652 1101 52-53 0.29641650588656177 1101 54-55 0.13471642794900163 1101 56-57 -0.02227336590773632 1101 58-59 -0.0017328075057250203 1101 60-61 0.04086940531834671 1101 62-63 -0.07824334625635743 1101 64-65 0.025412870105157026 1101 66-67 -0.02132914656145246 1101 68-69 -0.25768499463920236 1101 70-71 -0.24727611573343466 1101 72-73 -0.07493625449588137 1101 74-75 0.04909034306687943 1101 76-77 0.3843264724124502 1101 78-79 0.12019072858032942 1101 80-81 -0.015406870330249234 1101 82-83 -0.1828293863295265 1101 84-85 0.010722049260468935 1101 86-87 -6.379991763090231E-4 1101 88-89 -0.03724102337201529 1101 90-91 -0.05506097916068953 1101 92-93 0.017055959093639217 1101 94-95 -0.2454269821551307 1101 96-97 -0.272786941130164 1101 98-99 -0.31312232078388647 1101 100-101 -0.7488564718484518 1102 1 0.3853462448824061 1102 2 0.45295742090364755 1102 3 0.21646750626472056 1102 4 0.05131393416213825 1102 5 0.08458447455110019 1102 6 0.05523633891733937 1102 7 -0.05097480954612621 1102 8 -0.14858825219677385 1102 9 0.019155053658927557 1102 10-11 -0.004303023955415597 1102 12-13 0.06252867559616959 1102 14-15 0.1347467892401184 1102 16-17 0.3001727777097898 1102 18-19 0.1871785777493642 1102 20-21 0.23291664979913662 1102 22-23 0.10930637555873801 1102 24-25 0.10931880153872697 1102 26-27 -0.2846632414281913 1102 28-29 -0.05940017505503903 1102 30-31 -0.007160904197057505 1102 32-33 0.21436243561763035 1102 34-35 0.12725887292675964 1102 36-37 0.15772281095910756 1102 38-39 0.17437094650560425 1102 40-41 0.3010310633365876 1102 42-43 0.1808980398462765 1102 44-45 0.02265942070697946 1102 46-47 -0.05191046154756407 1102 48-49 -0.33199065506215675 1102 50-51 -0.16425347587989592 1102 52-53 -0.23357287840217822 1102 54-55 -0.09452089523736618 1102 56-57 0.06527867865067094 1102 58-59 -0.21068998620813062 1102 60-61 0.015784617494709607 1102 62-63 -0.29115384340251893 1102 64-65 -0.1273775454241104 1102 66-67 -0.11218653757808283 1102 68-69 -0.3689879697956826 1102 70-71 -0.479243193241917 1102 72-73 -0.48854228752866646 1102 74-75 -0.9635657906083601 1102 76-77 -0.8130292504209784 1102 78-79 -0.6410798479768722 1102 80-81 -0.5188638698063563 1102 82-83 -0.2694784060995623 1102 84-85 -0.17990579423361552 1102 86-87 -0.23531070439041812 1102 88-89 -0.1503741747217262 1102 90-91 -0.42386388538398023 1102 92-93 -0.10729262226131198 1102 94-95 -0.27262801891030364 1102 96-97 -0.031748255823963234 1102 98-99 -0.4474351947464399 1102 100-101 -0.6282647493458988 1103 1 0.30770896987671037 1103 2 0.3840351151392376 1103 3 0.1822199555697992 1103 4 0.09997251242598537 1103 5 -0.06883885650380961 1103 6 0.02825126259016031 1103 7 -0.057211993414888695 1103 8 0.08626023265171057 1103 9 0.04403258840488178 1103 10-11 0.01443700794251157 1103 12-13 0.07938900824597539 1103 14-15 0.02365229163245175 1103 16-17 -0.02797714254539585 1103 18-19 0.09771486164090959 1103 20-21 0.07588344177908368 1103 22-23 -0.09207776661487799 1103 24-25 -0.10506659376771665 1103 26-27 -0.053944398867251664 1103 28-29 -0.004055848329137746 1103 30-31 -0.0038215900024809457 1103 32-33 -0.15762577354163199 1103 34-35 -0.2646441801605022 1103 36-37 -0.19346766523137404 1103 38-39 -0.1013823572082373 1103 40-41 0.06979957552615446 1103 42-43 -0.02636016247771522 1103 44-45 -0.07351140408226797 1103 46-47 0.10528650814940477 1103 48-49 0.36696554224930367 1103 50-51 0.11387793218572284 1103 52-53 0.0761858935276436 1103 54-55 0.2838112988842951 1103 56-57 0.07585910699425824 1103 58-59 0.10722838927603817 1103 60-61 0.3005946539494815 1103 62-63 0.3401972616327953 1103 64-65 0.11024776785910717 1103 66-67 0.32744236422187 1103 68-69 0.1249101277208382 1103 70-71 0.10883210864917459 1103 72-73 0.035495078613962505 1103 74-75 -0.176776373884735 1103 76-77 -0.01124496014984544 1103 78-79 0.06990927255172252 1103 80-81 0.09370448289357469 1103 82-83 -0.0023902311166494883 1103 84-85 -0.13573286190279177 1103 86-87 -0.10834556426738118 1103 88-89 0.1542154242757654 1103 90-91 -0.23465506788568646 1103 92-93 -0.022962478720639723 1103 94-95 -0.10970651059405867 1103 96-97 -0.2597757108251031 1103 98-99 -0.33085824099843464 1103 100-101 0.09274943383933376 1104 1 0.3048862206085339 1104 2 0.37313741117444366 1104 3 0.11648151042174248 1104 4 0.05492676098722882 1104 5 0.08064488406213144 1104 6 0.17127429263974392 1104 7 0.046395848550979224 1104 8 -0.08078479194155364 1104 9 0.04616956393110172 1104 10-11 -0.05926811183764613 1104 12-13 0.0029557286792964987 1104 14-15 0.002162432620572474 1104 16-17 0.030287488953895547 1104 18-19 0.13823712003946298 1104 20-21 0.007726119557517563 1104 22-23 0.04214968623973192 1104 24-25 8.873162627480724E-4 1104 26-27 0.1266468351769987 1104 28-29 -0.0061100580589084075 1104 30-31 0.08955254126780687 1104 32-33 0.017011923851683264 1104 34-35 -0.04412498347411997 1104 36-37 0.008149814904832908 1104 38-39 -0.21344040030120937 1104 40-41 -0.07145985853004078 1104 42-43 -0.15629810679935474 1104 44-45 -0.09372679428877007 1104 46-47 -0.02914879264514525 1104 48-49 -0.15205627805059407 1104 50-51 -0.2003676171302473 1104 52-53 -0.3422088980767981 1104 54-55 -0.02975781117802967 1104 56-57 0.052880085945616884 1104 58-59 -0.0327683996599788 1104 60-61 -0.27322310198974975 1104 62-63 -0.19414618779667592 1104 64-65 -0.22199405263993555 1104 66-67 -0.30737316139694215 1104 68-69 -0.25314349455847207 1104 70-71 -0.429776579643633 1104 72-73 -0.3867025835323261 1104 74-75 -0.6776942652362692 1104 76-77 -0.35282562506412063 1104 78-79 -0.36973579286437186 1104 80-81 -0.30906624215969103 1104 82-83 -0.2959511766131975 1104 84-85 -0.29196730442225416 1104 86-87 -0.06291523190981252 1104 88-89 -0.2127762070443069 1104 90-91 -0.5272016656513827 1104 92-93 -0.4111054382761665 1104 94-95 -0.34469291957906734 1104 96-97 -0.48845698409909133 1104 98-99 -0.36980732696533636 1104 100-101 -0.15713419620626468 1105 1 0.321260541556196 1105 2 0.3952145308822743 1105 3 0.25407230652774615 1105 4 0.033545837280122726 1105 5 -0.032093151620195215 1105 6 0.09507413824881183 1105 7 0.10563651162215848 1105 8 0.08146544937591216 1105 9 0.07150038764982725 1105 10-11 0.058604532623029115 1105 12-13 0.011772469776673233 1105 14-15 0.04829684677751089 1105 16-17 -0.054880923862896225 1105 18-19 0.016628136629300627 1105 20-21 0.07514088126387009 1105 22-23 -0.0072959040182283275 1105 24-25 -0.11225498332618855 1105 26-27 0.06318458585000997 1105 28-29 0.13843943259468716 1105 30-31 -0.03218967289671326 1105 32-33 0.031831885998478526 1105 34-35 0.0013615634851475988 1105 36-37 0.2617970068054447 1105 38-39 0.08098911736427539 1105 40-41 0.2934964039031911 1105 42-43 0.12383326917391457 1105 44-45 -0.02767895399848186 1105 46-47 0.10737223887437608 1105 48-49 0.34346416438000915 1105 50-51 -0.02289641521903718 1105 52-53 -0.033582467968734875 1105 54-55 0.2387302418112398 1105 56-57 0.15759880578092833 1105 58-59 0.036364646922457666 1105 60-61 0.029168912524568213 1105 62-63 -0.15852328830361273 1105 64-65 0.0887986834103458 1105 66-67 0.12139475454751292 1105 68-69 0.1459649043344058 1105 70-71 -0.029410325687528882 1105 72-73 -0.1092064335092715 1105 74-75 0.019063394552468083 1105 76-77 0.06173101041522955 1105 78-79 0.22885823082196666 1105 80-81 -0.15868659981896016 1105 82-83 -0.1604313350271731 1105 84-85 0.044558559977573964 1105 86-87 -0.26905912896162576 1105 88-89 -0.22711810589603942 1105 90-91 -0.4790792396499626 1105 92-93 -0.45050320914278075 1105 94-95 -0.39162084043477563 1105 96-97 -0.21393632092341974 1105 98-99 -0.06687324614651757 1105 100-101 0.1986983719210187 1106 1 0.2282465237257476 1106 2 0.42385218450303697 1106 3 0.18523628197299757 1106 4 0.07271631588480432 1106 5 -0.13335624427929815 1106 6 -0.12017306780879267 1106 7 0.1247118999484087 1106 8 0.16158644854731818 1106 9 -0.006175783949494473 1106 10-11 -0.03352765460353879 1106 12-13 0.028177795159884056 1106 14-15 0.20811840576119778 1106 16-17 0.15934726277394873 1106 18-19 0.03476026652322162 1106 20-21 0.0196053466301791 1106 22-23 0.03712870151273506 1106 24-25 0.04584727899247554 1106 26-27 0.04740104659694566 1106 28-29 0.17244997361004977 1106 30-31 0.18433591452396314 1106 32-33 0.26527060218415954 1106 34-35 -0.10026804855033333 1106 36-37 -0.0103419238316107 1106 38-39 0.20754136446249305 1106 40-41 0.2631176126117438 1106 42-43 0.18014893722885006 1106 44-45 0.1302779291262013 1106 46-47 0.26624439664287536 1106 48-49 0.1973976612478694 1106 50-51 -0.06410288507094464 1106 52-53 0.294748592534944 1106 54-55 -0.06722197825873621 1106 56-57 -0.07809840689722591 1106 58-59 -0.12589696191331967 1106 60-61 0.024877837442012662 1106 62-63 -0.2867381819583841 1106 64-65 0.012683461184941791 1106 66-67 -0.06490834127853518 1106 68-69 -0.2409491936252337 1106 70-71 -0.3221357692305773 1106 72-73 -0.14397048922021582 1106 74-75 0.0801479096889679 1106 76-77 0.002995768451015124 1106 78-79 0.0059830535344289615 1106 80-81 -0.10030422660258509 1106 82-83 0.1619507815573833 1106 84-85 0.03620164518342861 1106 86-87 0.21246201517945806 1106 88-89 0.10043077359211594 1106 90-91 -0.02926782308328768 1106 92-93 0.09067459438918135 1106 94-95 0.25310721856870444 1106 96-97 0.2431781819138621 1106 98-99 0.271304695256795 1106 100-101 0.7275339596556556 1107 1 0.6575305577446855 1107 2 0.5693012465695055 1107 3 0.42493107659269214 1107 4 0.2730359974096288 1107 5 0.15012486636268108 1107 6 0.4148592019300352 1107 7 0.21909636221995754 1107 8 0.35123198406413536 1107 9 0.4674454638137462 1107 10-11 0.5025279981298425 1107 12-13 0.4390529987306664 1107 14-15 0.7389455565744711 1107 16-17 0.6908379035444057 1107 18-19 0.6499803240256838 1107 20-21 0.5842483689274616 1107 22-23 0.6714220847083467 1107 24-25 0.41673313861408445 1107 26-27 0.5523148934313014 1107 28-29 0.6570298638326264 1107 30-31 0.575350652043312 1107 32-33 0.6912572921735105 1107 34-35 0.5415519245079565 1107 36-37 0.7264475890059146 1107 38-39 0.6485711812990544 1107 40-41 0.7932682047262674 1107 42-43 0.585911246978128 1107 44-45 0.5952014267235626 1107 46-47 0.5313458301833052 1107 48-49 0.4292592532216517 1107 50-51 0.35623408740428175 1107 52-53 0.6442519803548805 1107 54-55 0.9253961933237846 1107 56-57 0.739979683860156 1107 58-59 0.37228748829476643 1107 60-61 0.6282726943895582 1107 62-63 0.572913586342878 1107 64-65 0.49756192188824144 1107 66-67 0.5977683380547667 1107 68-69 0.7659430596268706 1107 70-71 0.6049999647300552 1107 72-73 0.7620190155750102 1107 74-75 0.7425571824637665 1107 76-77 1.0362516946226776 1107 78-79 1.1659786302680466 1107 80-81 1.143031720479069 1107 82-83 1.1213683177980087 1107 84-85 0.9844202751771967 1107 86-87 1.0841239569933983 1107 88-89 1.4842908777399373 1107 90-91 1.489910052542502 1107 92-93 1.8344639262748998 1107 94-95 1.8908175750437621 1107 96-97 2.002293126522506 1107 98-99 1.7938462639078985 1107 100-101 1.6524821873332414 1108 1 0.3139594995826194 1108 2 0.42897536431289396 1108 3 0.014933532991612708 1108 4 0.2026983790248451 1108 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-0.9490586750011616 2316 82-83 -0.9719976196319919 2316 84-85 -1.0425552593059884 2316 86-87 -1.0827156856059688 2316 88-89 -1.299734359324205 2316 90-91 -1.5311569227430937 2316 92-93 -1.5314349263595588 2316 94-95 -1.3305069974941297 2316 96-97 -1.6612798842542666 2316 98-99 -1.9251250892865563 2316 100-101 -2.8979931853738954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 8.0 5 23.0 6 55.0 7 131.0 8 297.0 9 575.0 10 1028.0 11 1747.0 12 2722.0 13 3775.0 14 4640.0 15 6598.0 16 8722.0 17 11665.0 18 9684.0 19 5425.0 20 4641.0 21 4586.0 22 4867.0 23 5341.0 24 6077.0 25 7156.0 26 8661.0 27 10728.0 28 13348.0 29 16749.0 30 20346.0 31 25135.0 32 31934.0 33 41174.0 34 54550.0 35 74613.0 36 104543.0 37 136937.0 38 89414.0 39 6014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54193518618207 19.618365546353612 14.887714681614739 19.951984585849576 2 15.43775204711649 22.684136867714898 39.23763129936462 22.640479785803986 3 21.24483678008482 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22-23 21.025566996637664 25.96359728250509 31.93001262601844 21.080823094838806 24-25 21.70409744803941 26.065941534491664 32.27558798420807 19.954373033260858 26-27 21.379418949915976 25.380725735878666 33.35405404098694 19.88580127321842 28-29 20.14003044560542 27.111162375641307 32.886364954953216 19.862442223800063 30-31 20.508458538209332 26.251686147585314 32.43909666002913 20.800758654176224 32-33 20.533320808228442 26.54737425220634 32.31774072894729 20.60156421061793 34-35 21.689007135550597 25.77942991571472 32.232092226870584 20.2994707218641 36-37 21.6883930489391 25.432058345921583 32.7323438176683 20.147204787471015 38-39 21.151007682547707 26.138620152822128 32.83216848421807 19.878203680412092 40-41 20.96382263309563 26.606002668958027 32.38688010963173 20.043294588314605 42-43 20.39014955272338 27.222394915898178 31.225019859772736 21.162435671605706 44-45 21.232568259208865 26.379654507905155 30.86877240483772 21.519004828048267 46-47 22.09367540447352 27.282484970382814 30.122847780921227 20.500991844222437 48-49 22.255110273597886 27.235105742184697 29.680618261595164 20.82916572262225 50-51 21.11341265724514 27.85037356188861 30.528056338534032 20.508157442332227 52-53 21.705742261319305 28.450219368528074 29.199498270469793 20.644540099682825 54-55 20.848671399391154 27.691895353230855 29.590212005517902 21.86922124186009 56-57 21.98819979941593 27.687301591687092 28.97334663192823 21.351151976968747 58-59 23.021396394840774 27.11577592149268 28.69795429523406 21.164873388432493 60-61 21.62978353638741 27.457129318669416 30.403059694997538 20.51002744994564 62-63 21.684107282234056 27.298465916547514 30.17157421413622 20.845852587082202 64-65 21.241960554039586 28.59120583054126 29.441953645815346 20.724879969603812 66-67 21.53265639213503 27.698549579461616 28.90673835308647 21.86205567531688 68-69 21.185956609456795 28.138375648471815 29.09788255088488 21.577785191186514 70-71 22.107152033551316 28.096396493467385 28.547007484397145 21.24944398858415 72-73 21.874974099133322 27.31823117110596 29.44058272115196 21.366212008608755 74-75 21.203071083029197 29.17763712264164 28.672190343051803 20.947101451277362 76-77 21.77371340162038 27.746320304459836 28.75985108543248 21.7201152084873 78-79 21.710874882655215 27.93610641194703 28.87085822124992 21.482160484147833 80-81 22.10237560194834 28.07312286412391 28.559240835291185 21.265260698636563 82-83 21.797075219496694 27.951893785014125 29.028895505066902 21.222135490422275 84-85 21.34982572332075 28.193449186225305 28.871076702461423 21.585648387992524 86-87 21.41479059698286 28.385266460495295 28.45710638456602 21.742836557955822 88-89 21.503736022302135 28.340570473160405 27.895218651439308 22.260474853098152 90-91 21.508351520351844 27.593351392541837 28.480767357462483 22.41752972964384 92-93 21.80709285499674 28.350146256894103 28.41410558815302 21.42865529995614 94-95 21.444503158469153 27.97260472682437 28.517056424177746 22.065835690528736 96-97 20.726906495890983 27.911490817306994 29.165092296650684 22.19651039015134 98-99 21.116512902129493 28.40526971653722 27.8559725019825 22.622244879350788 100-101 20.618290358701802 28.287201833056212 28.184068243627376 22.91043956461461 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 540.0 1 404.5 2 428.5 3 564.5 4 450.5 5 367.5 6 567.0 7 1105.0 8 1685.5 9 1930.0 10 1720.0 11 1492.0 12 1465.0 13 1524.0 14 1641.0 15 1750.0 16 1850.0 17 1925.0 18 2075.0 19 2171.5 20 2289.0 21 2569.0 22 2876.0 23 3258.5 24 3649.5 25 4212.0 26 5181.0 27 6291.0 28 7370.5 29 8617.5 30 10130.0 31 11762.0 32 13781.0 33 15477.5 34 16720.5 35 17646.5 36 19252.0 37 20730.5 38 20835.0 39 20946.5 40 21095.0 41 21500.5 42 21842.5 43 22315.0 44 23247.0 45 23887.5 46 24221.0 47 29147.0 48 43815.0 49 42240.0 50 26915.0 51 21178.5 52 18764.5 53 17996.0 54 17450.5 55 16132.0 56 14374.5 57 13123.5 58 12328.0 59 11154.5 60 9318.0 61 7660.0 62 6375.0 63 4881.0 64 3451.5 65 2527.0 66 1867.0 67 1447.5 68 1059.0 69 726.0 70 565.5 71 459.0 72 403.0 73 395.5 74 303.0 75 236.5 76 187.5 77 103.0 78 65.5 79 51.0 80 41.5 81 30.5 82 18.0 83 13.5 84 8.5 85 5.0 86 2.5 87 1.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.20552993863912 2 0.012570553960829602 3 0.006078070046994532 4 0.014642623295032284 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0041441386684053635 10-11 2.0720693342026814E-4 12-13 2.762759112270242E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0016576554673621451 24-25 0.0 26-27 6.906897780675605E-5 28-29 0.0 30-31 6.906897780675605E-5 32-33 0.004765759468666168 34-35 4.834828446472924E-4 36-37 0.0033843799125310467 38-39 6.906897780675605E-5 40-41 0.004972966402086435 42-43 0.012294278049602576 44-45 0.00262462115665673 46-47 0.001381379556135121 48-49 0.010774760537853943 50-51 6.216208002608044E-4 52-53 0.0024864832010432175 54-55 0.01222520907179582 56-57 0.0038678627571783392 58-59 9.669656892945848E-4 60-61 0.007045035736289117 62-63 0.004006000712791851 64-65 0.019201175830278184 66-67 0.0028318280900769977 68-69 6.906897780675605E-5 70-71 0.0011051036449080968 72-73 0.0 74-75 0.0 76-77 0.0012432416005216087 78-79 0.011810795204955286 80-81 0.016369347740201184 82-83 0.013675657605737699 84-85 0.008978967114878287 86-87 0.011396381338114749 88-89 0.029906867390325373 90-91 0.02534831485507947 92-93 0.0022792762676229496 94-95 0.009186174048298554 96-97 0.010981967471274213 98-99 0.011051036449080969 100-101 0.013192174761090405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 723914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.22184765125249 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5541113949184 48.06452896619466 2 10.470720920785679 12.192494364975321 3 2.9643864578533337 5.177761701857183 4 1.2085629637997426 2.8145907502117917 5 0.6488576556023802 1.888884578591532 6 0.43629137639209176 1.5241014028713373 7 0.2716468483021436 1.107104699175321 8 0.2097711039441648 0.977060900437777 9 0.16398426891778456 0.8592720409919945 >10 0.9524960521985206 10.292529828032851 >50 0.07188478480431851 2.8925582915360515 >100 0.03892750562894489 4.123931655839046 >500 0.00429874295274827 1.7309695263685758 >1k 0.0033434667410264323 3.789250665322229 >5k 7.164571587913784E-4 2.5649606275942745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7726 1.0672538450699944 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5638 0.7788217937489812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5083 0.7021552283834821 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.4336150426708145 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3089 0.4267081448901389 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2464 0.3403719226316938 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2336 0.3226902643131643 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2291 0.31647405631055625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2219 0.30652812350638337 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1703 0.23524893840981112 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1611 0.222540246493368 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1586 0.2190867976030302 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1518 0.20969341662131136 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1458 0.20140513928450063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 0.1950507933262791 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1351 0.18662437803385484 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1075 0.1484983022845255 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 983 0.1357896103680824 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.13288871330019864 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 930 0.12846829872056625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 879 0.12142326298427715 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 803 0.11092477835765022 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 763 0.10539926013310973 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 738 0.10194581124277192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.381379556135121E-4 0.0 0.0 0.0 0.0 3 1.381379556135121E-4 0.0 0.0 0.0 0.0 4 1.381379556135121E-4 0.0 0.0 0.0 0.0 5 4.144138668405363E-4 0.0 0.0 0.0 0.0 6 6.906897780675605E-4 0.0 0.0 0.0 0.0 7 6.906897780675605E-4 0.0 0.0 0.0 0.0 8 6.906897780675605E-4 0.0 0.0 0.0 0.0 9 6.906897780675605E-4 0.0 0.0 0.0 0.0 10-11 6.906897780675605E-4 0.0 0.0 0.0 0.0 12-13 6.906897780675605E-4 0.0 0.0 0.0 0.0 14-15 6.906897780675605E-4 0.0 0.0 0.0 6.906897780675605E-5 16-17 6.906897780675605E-4 0.0 0.0 0.0 1.381379556135121E-4 18-19 6.906897780675605E-4 0.0 0.0 0.0 1.381379556135121E-4 20-21 6.906897780675605E-4 0.0 0.0 2.0720693342026814E-4 1.381379556135121E-4 22-23 6.906897780675605E-4 0.0 0.0 4.8348284464729235E-4 1.381379556135121E-4 24-25 9.669656892945847E-4 0.0 0.0 0.0015195175117486332 1.381379556135121E-4 26-27 0.0010360346671013407 0.0 0.0 0.0017957934229756573 1.381379556135121E-4 28-29 0.001243241600521609 0.0 0.0 0.0030390350234972665 1.381379556135121E-4 30-31 0.001243241600521609 0.0 0.0 0.006492483913835069 1.381379556135121E-4 32-33 0.001243241600521609 0.0 0.0 0.012363347027409332 1.381379556135121E-4 34-35 0.001312310578328365 0.0 0.0 0.018924899919051157 1.381379556135121E-4 36-37 0.001381379556135121 0.0 0.0 0.02479576303262542 1.381379556135121E-4 38-39 0.0016576554673621451 0.0 0.0 0.031633591835494274 1.381379556135121E-4 40-41 0.0016576554673621451 0.0 0.0 0.0410269728172131 1.381379556135121E-4 42-43 0.0016576554673621451 0.0 0.0 0.053942871667076474 2.0720693342026814E-4 44-45 0.0016576554673621451 0.0 0.0 0.06692783949474662 2.762759112270242E-4 46-47 0.0016576554673621451 0.0 0.0 0.0820539456344262 3.453448890337802E-4 48-49 0.0016576554673621451 0.0 0.0 0.09496984448428958 5.525518224540484E-4 50-51 0.0016576554673621451 0.0 0.0 0.11285870973623939 5.525518224540484E-4 52-53 0.001726724445168901 0.0 0.0 0.15146826833021604 5.525518224540484E-4 54-55 0.0017957934229756573 0.0 0.0 0.20485858817483846 5.525518224540484E-4 56-57 0.0017957934229756573 0.0 0.0 0.2523089759280799 5.525518224540484E-4 58-59 0.0017957934229756573 0.0 0.0 0.32414071284710616 5.525518224540484E-4 60-61 0.0017957934229756573 0.0 0.0 0.40660907234837285 5.525518224540484E-4 62-63 0.0017957934229756573 0.0 0.0 0.45412852907942103 5.525518224540484E-4 64-65 0.0019339313785891694 0.0 0.0 0.4930834325624315 5.525518224540484E-4 66-67 0.0020720693342026813 0.0 0.0 0.6226568349279058 5.525518224540484E-4 68-69 0.0022102072898161937 0.0 0.0 0.745185201557091 5.525518224540484E-4 70-71 0.0022102072898161937 0.0 0.0 0.7990590042463608 6.216208002608045E-4 72-73 0.0022102072898161937 0.0 0.0 0.900037849799838 6.906897780675605E-4 74-75 0.0022102072898161937 0.0 0.0 1.0513679801744407 6.906897780675605E-4 76-77 0.0022792762676229496 0.0 0.0 1.2057371455725403 6.906897780675605E-4 78-79 0.0023483452454297056 0.0 0.0 1.3344817202043338 6.906897780675605E-4 80-81 0.0023483452454297056 0.0 0.0 1.5050130264092143 6.906897780675605E-4 82-83 0.0023483452454297056 0.0 0.0 1.6711929870122693 6.906897780675605E-4 84-85 0.002417414223236462 0.0 0.0 1.8607182621140081 6.906897780675605E-4 86-87 0.002486483201043218 0.0 0.0 2.206753840925856 6.906897780675605E-4 88-89 0.002486483201043218 0.0 0.0 2.6324950201267 6.906897780675605E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCGAG 50 0.0016173148 37.990936 7 TACCTGG 635 0.0 37.39782 2 GAGTCGA 65 1.3295635E-4 36.529747 6 TGTACGC 55 0.002580266 34.54199 2 TCGCGCT 35 2.0548057E-4 33.925167 60-61 GTACCTG 850 0.0 29.40118 1 ACGACGG 65 0.005849476 29.223797 6 CCGCGAT 45 8.959335E-4 26.383509 22-23 ACAACGC 95 0.0012273277 24.995764 3 GAGTACT 5170 0.0 23.790264 12-13 CTCGCGC 50 0.0016548188 23.747616 60-61 GTACTTT 5535 0.0 22.092741 14-15 AGTACTT 5265 0.0 21.782553 12-13 ACCTGGG 1070 0.0 21.74865 3 CGCGGAA 55 0.0028776673 21.59098 82-83 ATTCGCG 55 0.0028782443 21.590235 80-81 CGCGATC 55 0.00288113 21.586508 22-23 CGATCGT 55 0.0028817074 21.585762 24-25 GTGTAGC 360 0.0 21.359833 1 ACGATAC 135 3.7984143E-4 21.107536 3 >>END_MODULE